Giggling Villain

Cronus laughing like a baby. Well, you are what you eat.

"I HAVE CHORTLES!"

— Fawful, Mario & Luigi: Bowser's Inside Story

Not all villains manage to develop an Evil Laugh. Some, for reasons known only to themselves, prefer to giggle. This can become highly disturbing, making the villain more evil or creepier than if they'd just done a straight-out "Muhahaha!" It's especially creepy when the villain is male, but a giggle from that otherwise dark and grim lady or Cute and Psycho Enfant Terrible is also a very bad sign, since it's generally amusement at the prospect of hurting you. Badly. And Your Little Dog, Too!.

Oddly enough, it can work out for those bad guys who wouldn't be able to carry off a full-throated, maniacal laugh. Or even for those who can, as it provides some contrast between appearance and laugh. One suspects that these villains know exactly how unsettling it is... and derive much pleasure from creeping people out. See also Laughing Mad, The Hyena, and Creepy High-Pitched Voice.

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Examples:

Anime & Manga

• Kuroudo Akabane, the token Psycho for Hire from Get Backers sometimes pulls this off.
• Ditto Alan Gabriel from The Big O.
• Suigintou from Rozen Maiden.
• Nui Harime of Kill la Kill is this. Most of the time.
• Mad Scientist Jail Scaglietti of Magical Girl Lyrical Nanoha StrikerS. Even when Fate was bearing down on him with her BFS, he was still giggling his mad little giggle.
• Naruto: Orochimaru. "Fufufufu" indeed.
  • Kabuto's also been known to giggle on occasion.
• Mukuro Rokudo, from Reborn! (2004) has an unsettling giggle similar to Orochimaru above.
  • Recent chapters have shown that the First Mist Guardian Daemon Spade has a similar laugh to Mukuro.
  • Belphegor's "Ushishishi" sounds like a giggle.
  • Byakuran. He hardly even opens his mouth to laugh.
• Suzu giggles creepily in the Peacemaker Kurogane manga after going insane.
• Kogarashi from Kamen no Maid Guy, a Sociopathic Hero example. His chuckle, "kukuku", is normally used only by females, which only helps underline its wrongness.
• Inuyasha: Naraku "kukuku"s with the best of them.
• Death Note: Light Yagami by both Mamoru Miyano and Brad Swaile. Creepy indeed.
• Being the only markedly Ax-Crazy villain in Negima! Magister Negi Magi, Tsukuyomi does this often when fighting Setsuna, starting with an inhibited giggle as the fight beings, to a straight-out cackle as she gets carried away with the fight. In the manga, Fate also does this a few times.
• Yami Bakura from Yu-Gi-Oh!, at least in the Japanese version. Especially back when he had a female voice actor.
• Saiou/The Light from GX even more so.
• Higurashi: When They Cry's main villain Miyo Takano practices this in the beginning before going completely psychotic.
• Kururu from Sgt. Frog, as befitting of his status as a Jerkass and a Mad Scientist, has a creepy high-pitched "KU KU KU KU KUUUU!"
• Ashley in Betrayal Knows My Name.
• Hansel and Gretel of Black Lagoon, due to being Creepy Twins.
• Masane from the Witchblade anime, despite being the protagonist.
• For another protagonist, try Lelouch Lamperouge of Code Geass. Nevertheless, his laughter often starts as quiet chuckles until it evolves into full blown fits of laughter. In R2, Suzaku's laugh starts off as manic giggles when it finally clicks for him as to what he had done to the Tokyo Settlement.
• Izaya of Durarara!! (who incidentally has the same English voice actor as Lelouch) has a rather creepy, unhinged laugh/giggle that he demonstrates on a couple of occasions.
• Dragon Ball Z:
  • Frieza does this occasionally with a distinct "Oh hohohoho" laugh in the Japanese version.
  • The Cell Jr.'s are straight examples.
• DarkKnightmon from Digimon Fusion enjoys BOTH giggling and laughing.
  • In the Japanese version of Our War Games, the only sound Diablomon makes is a creepy childish giggle.
• In the 4Kids dub of Pokémon: The Series, Team Rocket's James had a tendency to let out a delightful "Heeheehoohoo!"
• Zeus in King of Thorn, and comes across as Faux Affably Evil.
• Russia from Hetalia: Axis Powers..."ufufufu~"
• Takasugi from Gintama, more often than not.
• Aboshi from The Hakkenden could outgiggle anybody.
• Diva of Blood+ is very prone to letting out a childish giggle, as shown in her first in-person appearance in episode 24.
• Eto from Tokyo Ghoul is a creepy little Ghoul girl prone to giggling at the expense of others. Made even more jarring by her Voice of the Legion, especially when that cute, girlish giggling is coming from her massive, barely humanoid Kakuja form.
• Clementine in Overlord (2012) giggles a great deal, sometimes barely able to speak straight from the effort of holding them back and other times breaking off mid-word for a few giggles. The fact that the giggles are usually triggered by somebody else's suffering doesn't help.
• Nao Sadatsuka of Food Wars! is more of a creepy Stalker with a Crush than a villain, but when she's alone she tends to go "Hihihihi!" out of nowhere, creeping everyone around her. It's sorta fitting considering that she has penchant for dressing and cooking as a Wicked Witch.
• Warlock, one of the two main antagonists in Prince Planet, is one of the more hammy examples of the trope. And BOY, is he a giggler.

Comic Books

• Batman: The Joker uses giggles when it suits him, full-on maniacal laughter when it doesn't.
  • However, it's Frank Gorshin's take on the Riddler with his maniacal giggle in Batman (1966) that is the real classic.
  • Mark Hamill states that he uses these quite often to change up his version of Mr. J to keep it from getting stale. In an interview, he even admitted to practicing his giggles while driving on the freeway.
    Hamill: One of the things that informs the Joker is his laugh. I remember reading the comic books, it was "Hahahahaha", but also a lot of "ho-ho, hee-hee, ho-ho-hee". His laugh should be like a musical instrument, it should sort of illustrate his mood. It could be ominous and intimidating, it could be gleeful and wild abandon, but I didn't want to just have one, rote laugh.
• Griffin in The League of Extraordinary Gentlemen. An already creepy characteristic is made worse by the fact that it seems to be coming from nowhere.
• As seen here, Linus' blanket qualifies in a series of Peanuts strips where it acted like a living creature. (Don't think "villain" is appropriate? Consider the next two strips...)

Fan Fiction

• Dungeon Keeper Ami:
  • Featured in A Fitting Punishment and played for both humor and Fridge Horror. Marda, a rebellious troll leader had insulted and defied Mercury on multiple occasions, had to be disciplined. Mercury sent her on what ammounted to a suicide mission, teleporting her off alone with no time at all to prepare, and with no backup (there was little on the island in question that could actually pose a threat to the powerful Marda, but most of her other minions wouldn't realize that). Mercury then scrys on her to discover that she is already plastered with mud and drenched in a torrential downpour. When Mercury comes back to herself, one of her advisors informs her that 'the giggle was a nice touch'. Except, Mercury dosen't remember giggling, and the nature of being a Keeper makes this worrisome.
  • And of course, there's always Mukrezar.

Film — Animation

• While Jafar from Aladdin has a full Evil Laugh once he becomes a sorcerer, his laughter shortly after discovering Aladdin has the lamp qualifies.
• Ratigan from The Great Mouse Detective. Vincent Price even went on record saying Ratigan was his favorite role of all time, mainly because he got to play one of these.
• Alameda Slim from Disney's Home on the Range has a very annoying, yet somewhat still creepy-sounding giggle.

• From The Road to El Dorado, Tzekel-Kan, the high priest. As he is already both comically hammy and scarily eager to perform human sacrifices and see the townspeople horribly killed, his giggling walks a fine line between creepy and hilarious.
• Steven Universe: The Movie: The villain Spinel often gives a high-pitched, manic laugh, as a remnant of her original silly, goofy personality before her Sanity Slippage. In the trailer, this is her only dialogue.

Film — Live-Action

• Peter Lorre belongs at the top of this list. Even his characters that were not villains were creepy, due in no small part to his distinctive voice and laugh.
• Next is Richard Widmark's Psycho for Hire Tommy Udo from Kiss of Death, who likes sending old women in wheelchairs on short but exciting trips down the stairs. So much so that he inspired Frank Gorshin's portrayal of the Riddler in Batman (1966).
• James Cagney's Cody Jarrett becomes this at the climax of White Heat.
• From Kill Bill, we have Gogo Yubari, normally a blankfaced psychopath in schoolgirl attire. Upon being asked to walk away from battle with the Bride, she gives a giggle that would be cute, if we hadn't already seen her utter insanity...and commences trying to bludgeon the Bride to death with a meteor hammer.
• Dr. Giggles... as should be apparent. He giggles whenever he "operates" on his victims or is hurt.
• Instead of having a Signature Roar like most of the Kaiju in the series, King Ghidorah from Godzilla often made some bizarre chirping sounds while slaughtering civilizations. Most people thought it just sounded stupid until they realized that he was really putting this trope into play.
• German agent Major Arnold Ernst Toht in Raiders of the Lost Ark. The moment he says "We... h-huh... we are... heh-heh... not thirsty..." in that stuttering, breathy sex offender's voice, you know nothing's beneath him. He lets more giggles rip when Indy and Marion are sealed in a tomb.
• Indiana Jones and the Temple of Doom: Lao Che. "To the poison you just drank!" Cue chortles.
• Repo! The Genetic Opera has Pavi Largo. His giggle is so cute and effeminate, it's hard to believe he's actually something of a monster.
• The aptly-named "Giggler" from the Charles Bronson film Death Wish 3.
• Asami from Audition giggles like a schoolgirl as she's cutting off her lover's feet with Razor Floss. It is very, very creepy.
• Renfield in Dracula (1931) has one of the goofiest laughs ever, and man is it ever creepy.
• In Live and Let Die, this is, unsurprisingly, the case with Kananga's henchman Tee Hee.
  Bond: Funny how the least thing amuses him.
• In King of New York, Laurence Fishburne plays The Dragon as a giggling villain. It seems to be something between a nervous tic and an occasional psychotic break.
• In the 2010 Clash of the Titans, Medusa laughs frequently as she battles Perseus' squad.
• Robert Englund's mercenary character in The Adventures of Ford Fairlane giggles maniacally every time he makes an appearance.
• The Toon Patrol in Who Framed Roger Rabbit fit this trope, particularly Psycho, who has a very distinct giggle compared to the other weasels.
• Esteban Rojo from A Fistful of Dollars whenever someone is being tortured.
• Romero from Escape from New York, added with a heavy dollop of Ambiguously Gay.
• Calder from Prince of Darkness. A Scary Black Man with a deep voice who makes high pitched giggles after being Touched by Vorlons makes for serious creepiness.
• What's Up, Tiger Lily? features a giggling black thug in the Japanese film's first fight scene. His creepy laughter is carried over into Woody Allen's Gag Dub.
• The Polite Leader from The Purge.
• Floyd, one of Valance's henchmen in The Man Who Shot Liberty Valance.
• Norman Bates of Psycho can rarely get through a sentence without a cute little nervous giggle. The tic seems to disappear as soon as he goes into full-blown psycho mode, though.
• Harmon James, Minister Edwidge Owens' right-hand man in The Purge: Election Year, does this after stabbing a man nine times.
• In Wonder Woman, General Ludendorf kills an uncooperative German High Command by locking them in a room with a canister of Dr. Maru's latest poison gas. Before the door gets barred, he tosses in a single gas mask for them to fight over. Maru wonders why he did that, noting that the mask is useless against her new gas. Ludendorff retorts "They don't know that!", then they both giggle like a pair of school kids after playing a prank.
• In Black Panther, Ulysses Klaue has a serious case of the giggles, even while captured and being interrogated.
• Maleficent is the dark and grim lady who giggles. She can do a full-blown Evil Laugh, but mostly it's this.

Literature

• Anita Blake: In the first novel, Guilty Pleasures, the character Nikolaos is both a cruel and considerably powerful one-thousand-year-old master vampire. Her character was noted several times to giggle as she tortured and tormented, not laugh.
• Stephen King's Randall Flagg is fond of this, especially in his The Dark Tower incarnation. Coupled with his Slasher Smile and deathly pallor, he manages to creep the hell out of the poor bit-part villains in Wizard and Glass.
• Discworld:

• Also used in Equal Rites, where the Things from the Dungeon Dimensions indulged in evil snickering during one of Esk's nightmares.
• Duke Felmet in Wyrd Sisters alternates between blank stares and manic giggling.

• The title characters in Duumvirate can do the Evil Laugh, but far more often they just do some version of this.
• This trope is at least half the reason many fans of Harry Potter hate Dolores Umbridge more than the series Big Bad. It's even worse in the movie version, thanks to Imelda Staunton's magnificent performance. There's tales of viewers with the powerful urge to somehow find a way to leap through the screen and punch her, if not worse.
• The men with no pain from the Inheritance Cycle madly giggle in battle.
• James Bond: In Live and Let Die, we see how Mr. Big's henchman Tee-Hee got his name, as he giggles while breaking James Bond's finger.
• In the second book in the Losing Christina series, the title character finds herself chased by one of these. She describes his laughter as "insane".
• Shadowthrone from the Malazan Book of the Fallen is dramatic, partially insane (albeit having Success Through Insanity) and prone to erupting into fits of the giggles at least once in most of his appearances. Though by the last book, while no one could ever really call him "good", he does prove rather less than villainous after it's revealed that he masterminded most of the plot's resolution in order to cement his place among the gods.
• Zabulon, leader of the Day Watch's Dark Others in the Night Watch (Series), breaks into a giggle every so often. It serves as a warning sign that you've just screwed up in trying to counter his tactics, or that events have progressed into the category of utterly weird.
• At the end of The Pilo Family Circus, Kurt Pilo, undergoing a Villainous Breakdown and mutating due to badly-restrained anger starts chuckling and giggling to himself: "Oh hoo hoo hoo hoo hoo... Well now, well now, isn't this something? Someone's having a laugh... oh ho ho ha ha... There's, ha ha, traitors, and I'm..."
• In A Series of Unfortunate Events, Count Olaf and his girlfriend, Esme, become an extreme and ridiculous version of this in The Grim Grotto. Working on perfecting their evil laughs, they ended up with things like "Ha ha heepa heepa ho!" "Tee hee terrycloth!" "Giggle giggle glandular problems!"
• Wilson Kemp from the Sherlock Holmes story "The Adventure of the Greek Interpreter". He terrifies the poor Greek interpreter far more than the threats of physical violence.
• Varys from A Song of Ice and Fire, who may very well not be a villain at all.
• Robin McKinley's Sunshine: One of the vampires in Bo's gang giggles; it's how the titular character recognizes him in a later encounter.
• The Word Is Murder: Robert Cornwallis does this during his Motive Rant at the end, when telling Anthony why and how he committed the murders.
  He giggled to himself. It was the most convincing portrayal of a lunatic I'd ever seen.

Live-Action TV

• Batman (1966): Frank Gorshin's version of the Riddler had a hysterical, unhinged giggle.
• Buffy the Vampire Slayer:
  • Warren Mears even giggles right before he shoots Buffy and kills Tara.
  • The Mayor does this on occasion too.
• Doctor Who:
  • The Master, a master of chuckling, chortling and guffawing. Anthony Ainley's Master was quietly chuckling to himself all through "Logopolis" and "Castrovalva". Seeing as how that's when Tom Baker left the series and Peter Davison came on as the Fifth Doctor, it was especially disturbing to many a fan.
  • An unnerving giggle is the only sound that the baby Weeping Angels in "The Angels Take Manhattan" make.
• Sheriff Rosco P. Coltrane from The Dukes of Hazzard is a Harmless Villain variant.
• In the Firefly episode "Objects in Space", River Tam's maniacal giggling manages to freak out Jubal Early.
  Jubal Early: You're somewhere on this boat. Somewhere with a com, playing games!
  [River's deranged giggling filters over Serenity's com system]
  Jubal Early: That's somewhat unsettling.
• The Flash (2014): Eobard Thawne quite often has a slight chuckle in his voice when he talks, but when he genuinely bursts out laughing, it's with a rather spectacularly wheezy and high-pitched fit of the giggles.
• Game of Thrones: Ramsay Bolton. Boy, the guy loves to laugh.
• Gotham: Jeremiah Valeska is prone to giggling to himself (and talking to himself) after undergoing serious sanity slippage between the fourth and fifth seasons. The change in his character is especially noticeable, because this version of the Joker started out as more of an unnervingly calm Cold Ham than would be expected from a character who is so famously unstable and Laughably Evil. By his later appearances, he has devolved to the point of talking to himself in the voice of his dead twin brother, apparently having developed a split personality, and giggling about how Batman is going to love the tunnel he dug for him from Gotham to the mainland.
  Jeremiah: It's a nice gift for him. He'll like it! [this time in Jerome's voice] No, he won't. [as himself, again] Come on, he's gonna love it! He's gonna... [randomly breaks down in giggles]
• GACKT gives Mr. Brain's Takegami Teijirou a bizarre laugh that's a mix between Giggling Villain and Evil Laugh. The result is...creepy, to say the least.
• Kamen Rider Zero-One: Jin loves causing chaos by hacking HumaGear and sending them on a rampage, as evident by how the ensuing chaos makes him chuckle maniacally like a kid in a candy store. Well, more like a gun store, actually. What makes him even creepier is that he is an adult combatant of a terroristic organization, but with the mind of a caffeinated five year old. It's mostly because he is a HumaGear whose development was deliberately stunted, so while he is acting in a perfectly human manner unlike the stilted regular HumaGear, there is whole different sort of Uncanny Valley to him.
• Kamen Rider Revice: Kagero, Daiji's inner demon, favors sinister, quiet giggles to go with his laid back, vaguely ominous persona in comparison to Vice, Ikki's inner demon, a bombastic bruise who loves to ham his Evil Laugh up to cartoonish proportions.
• Rumpelstiltskin does this a lot in Once Upon a Time. His Storybrooke counterpart Mr. Gold doesn't, though, even though he still remembers his true identity.
• The Red Dwarf episode "Demons and Angels" has the crew meeting their "good" and "evil" selves; when playing Evil Lister, Craig Charles came up with an impressively scuzzy giggle.

• In the Supernatural episode "All Hell Breaks Loose, Part One", the Acheri demon never speaks, but has a child-like laugh.

• The Ultra Series has a few creatures whose roars sound like this:
  • Ultraseven has Alien Perolynga, who giggles in a child-like voice when fighting Seven.
  • Ultraman Gaia has Gan-Q, an Eldritch Abomination resembling a walking mass of muscle and eyes who constantly giggles in a high-pitched voice, which its debut episode even references with its title, "The Ridiculing Eye". It's always giggling because it finds everything from our dimension hilarious, especially when things blow up.
  • Ultraman X has Greeza, a hole in the fabric of reality taking the form of a Humanoid Abomination. Its roar is a series of psychotic, echoing giggles, reflecting how it takes delight in destroying and consuming everything with its mere existence.
  • Ultraman Trigger: New Generation Tiga has Metsu-Orga, a misshapen, dinosaur-like Animalistic Abomination that laughs constantly in a deep voice as it eats everything in its path.

Professional Wrestling

• Mistress Glenda Lee, retainer of Monster Pain, and later, Los Rabiosos in the World Wrestling League. Relatively deep voice, very high pitched giggle.
• Along with Evil Laugh and Laughing Mad, this is the whole idea behind "The Laughing Man" Hugh Morrus' gimmick. In the Dungeon of Doom vignette that introduced him on the November 18 (taped October 26), 1995 WCW Saturday Night, "The Taskmaster" Kevin Sullivan told The Master, "I'm giving you laughter, Fatha! I'm giving you something you can smile about. I am giving you the man from The Isle of Nowhere. So without further ado, let me bring out the greatest gift of all, laughter, Mr. Hugh Morrus!"

Video Games

• Viktoria from Thief: The Dark Project qualifies. Her giggles can be heard echoing through the estates. Very creepy. She seems to have lost her mirth in the sequel, though.
• Some Uruks in Middle-earth: Shadow of Mordor don't speak at all. Instead, they simply giggle at you in a very unnerving way.
• Final Fantasy VI: KEFKA! KEFKA! KEFKA! (signature laugh)
• Chrono Trigger shares the same library of sound effects as Final Fantasy VI, so you can also hear Kefka's laugh emanating from villains like Dalton. (Curiously, the NPC Norstein Bekkler, the guy who runs those mini-games at the fair from his tent, also has this laugh.)
• Even though whether or not he can actually be considered a villain is arguable, Joshua in The World Ends with You is the true antagonist of the game and spends a lot of his time giggling playfully.
  • Konishi is a better fit. Uzuki too.
• Resident Evil:
  • Ramon Salazar, of Resident Evil 4, a Depraved Dwarf nobleman, is frequently seen giggling like the Psychopathic Manchild he is.
  • Alfred Ashford from Resident Evil – Code: Veronica, as well as sister Alexia, though she tends to extend it into a full-blown Evil Noblewoman's Laugh, as does Alfred himself when dressed as her. In The Darkside Chronicles, she may even be a bigger one than Alfred.

• Seymour in Final Fantasy X giggles during the cutscene before you fight him on Gagazet. The effect has been described as fairly creepy, especially considering what he's laughing to himself about: his decimation of most of the Ronso race, to Kimahri's face.
• Valtome from Fire Emblem: Radiant Dawn, who lets out a 'Uwee hee hee' every chance he gets, just like Kefka.
• Achenar from the original Myst does a very psychotic-sounding version of this, and it's very unnerving when a big guy like him could easily pull off a full-blown maniacal laugh. Even more vaguely disturbing is the fact that it's his brother, Sirrus, who laughs maniacally.
• Vaati from The Legend of Zelda: Four Swords series does this almost every freakin' time he shows up.
  • The Legend of Zelda: Majora's Mask the second form of the eponymous villain seems to giggle (and dance, and, in general, act weird). Skull Kid also counts for most of the game when he's being possessed by the mask.
    • Depending on whether or not you suspect him to be a villain, the Happy Mask Salesman may fit this trope. He and Vaati use the same laughing voice clip.
  • Zant in Twilight Princess turns out to be this after he breaks down in the battle against him.
  • Ohhhh boy, Ghirahim. He took this to a whole new level by interlacing it with over-the-top mannerisms, a tongue that puts Gene Simmons to shame, and a tendancy to get way too close, managing to be the first villain in the series to visibly creep the hell out of poor Link◊.
• Akechi Mitsuhide, the resident Combat Sadomasochist of Sengoku Basara, though he's also prone to break into a flat out Evil Laugh.
• Grandia II has it's fair share of laughing evils but evil brother Melfice will be giggling away throughout his fights with the heroes.
• Long since driven completely insane by his own powers, Nicholas Wrightson of Psi-Ops: The Mindgate Conspiracy has a nasty habit of giggling madly- often through other people's lips.
• Hugh Bliss from the end of Sam & Max: Freelance Police Season One.
• Yggdra Union has Nessiah, whose soft chuckle only manifests as a sign that his mind can't take much more strain. It's both heart-wrenching and really, really freaky.
• Lumine from Mega Man X8 manages one as he gloats and destabilizes X, which mutates into a louder cackle before he's shot out of it and into Glowing Eyes of Doom territory instead.
• Marx from Kirby Super Star (Ultra) sounds like an evil Elmo when you're fighting him.
  • Queen Sectonia in Kirby: Triple Deluxe giggles before and/or during practically all of her attacks, though she's got a proper laugh she uses occasionally, as well.
  • Francisca from Kirby Star Allies giggles whenever she uses her water gun. It helps represent her as the most feminine of the Three Mage Sisters.
• Abyss, the final boss of Marvel vs. Capcom 2. The boss' second form (after the titanic armored fiend but before the blood-red demon dog abomination) is an androgynous, humanoid puddle of green goo that giggles at you constantly while repeatedly juggling you with its energy cannon.
• Mario & Luigi: Superstar Saga: Fawful, provider of the page quote above, has a very distinctive giggle. Whenever he declares he has chortles, you can expect things are going well for him.
• Tira from the Soul Series fighting games (one half of the time). It can be cute or annoying, depending on your bias.
• BlazBlue:
  • Arakune, though sort of difficult to call a flat-out "villain" due to being so insane he can barely function beyond basic survival instincts, is prone to giggling like the loony he is, and especially so after blocking your blows.
  • Hazama is also rather prone to giggling when his Terumi persona slips through.
• Albedo from Xenosaga. He can Maniacal Laugh with the best of them, but his giggles are even more chilling for how happy he sounds.
• Star Control:
  • Although you can't actually hear it (even in the voice-acted version), Admiral ZEX's "Hee! Hee! Hee!" still counts. His voice actor for the Japanese 3DO dub replaces it with a perverted cackle.
  • Done to great effect by the cunning Plexor in Star Control 3.
• Chortlebot in Wario Land: Shake It giggles before the Boss Battle, and before a good deal of his attacks.
• In the Diablo II expansion Lord of Destruction, Baal has a cutscene intro where he giggles just before casting a spell that blows Cessuron's guardian to bits. From there, it scales up into full-blown maniacal laughter.
• Ace Attorney Investigations: Miles Edgeworth has Calisto Yew, who spends most of the time laughing at Edgeworth's seriousness, even when he's accusing her of murder.
• King Watinga, the giant boss fly from the "Gnat Attack" game within Mario Paint, giggles if you die while he is on the screen.
• The Infected from Survival Crisis Z have several sound effects dedicated to them, but for whatever reason females only use a distinct giggle that they repeat over and over, and several female infected can layer the sound over one another. Playing the game for an extended period of time quickly takes it over to Hell Is That Noise territory.
• Team Fortress 2: This Pyro. (Oh God, it's contagious!)
  • The "Schadenfreude" taunt features some pretty villainous laughing/giggling.
• Space Channel 5: Purge has a pretty impressive laugh in the Japanese version. And it's done by Akira Ishida, no less.
• Evil from Dark Blue in Custom Robo fits this perfectly. That creepy, high-pitched laugh that he makes every time you see him...
• In Enchanted Arms, the man who always follows the leader of the London Resistance. It's even Lampshaded by the party in that 'the Giggling Man' is the only thing they refer to him as. Oh, and no surprise, he and the Resistance Leader are evil.
• Lady Melodia from Baten Kaitos: Eternal Wings and the Lost Ocean giggles girlishly whenever things are going well for her, which is often.
• Touhou Chireiden ~ Subterranean Animism has Parsee's little smile and music-note emote when she tells Reimu that it's Nothing Personal, she just wants to kill everybody who can travel freely across her bridge.
• Knuckles from Sonic 3 & Knuckles used to set up traps for Sonic and Tails in Angel Island, laughing at them when he succeeded.
• The aptly named Hyena from Unreal Championship 2 is a nasty, vicious piece of work with an unsettling habit of giggling.
• Hermes, the villain of Sheep Happens, does a little "hehehehehe!" at the start of the race and occasionally throughout.
• Danganronpa: Trigger Happy Havoc's mascot character Monokuma has his distinctive "upupu~" which he likes to say at inappropriate times. The game's official dub turns this into a slightly-less awkward sounding "puhuhuhu" while the anime's dub converts it to a more cartoonish "nyuk-nyuknyuk!"
• Creepy Child Maria in Umineko: When They Cry. "Kihihihihihihi..."
  • As well as Bernkastel. *giggle* *giggle*
  • Let's not forget Ronove: "Pu ku ku ku..."
  • And Beatrice too: "YAHAHAHAHA!"
• White Face from Imscared has a childlike one, to fit its Affably Evil status. It is very unsettling.
• Wally Wingert took cues from Frank Gorshin when voicing the Riddler in the Batman: Arkham Series.
• Micah Bell of Red Dead Redemption II is the Van Der Linde Gang's resident psychopath who is prone to creepy chuckling whenever he's particularly pleased with himself. Even at the finale of the game when he gets shot by Dutch he still continues chuckling, more amused by it than anything.
• Ayano Aishi of Yandere Simulator will start giggling uncontrollably when her sanity is low or she's near Senpai. She can also use giggles to attract students' attention and to regain sanity, though she can also graduate to full on Evil Laugh while doing this.
• In Minecraft Dungeons, the Arch-Illager giggles whenever he summons a wave of enemies for you to fight off.
• Unknown of Tekken giggles and smiles quite a bit, controlling a possessed and pissed off Jun, and fulfills the villainess part by her hit effects being screaming faces (hence victims she possessed) and the walls of goo she creates are adorned with skeletons, making her at the very least murderous if not evil.
• A Tiny Sticker Tale: Although more of a general troublemaker than an actual villain, Rocky the raccoon always does a distinctive giggle after pulling one of his various pranks.

Web Animation

• RWBY:
  • Roman Torchwick lets out a number of maniacal cackles during his battle with Blake and Sun in Episode 16.
  • Volume 4 villain Tyrian fills this role better, though he sometimes strays into full Evil Laughter.
• Lifty and Shifty of Happy Tree Friends. A pair of kleptomaniac raccoons who almost always lets out giggling evil laughs.
• Cupcakes (Sergeant Sprinkles) has Pinkie Pie mutilate Rainbow Dash... all while keeping up her bubbly, giggly personality.

Webcomics

• General Protection Fault's Trudy, shown in one of her earliest appearances, while punting a puppy. This turns out to be some of her nicer behavior as events progress.
• Homestuck:
  • Terezi. Later subverted when its revealed she's not really a villain, she's just a very strange and funny girl.
  • Doc Scratch, Lil' Cal(sprite) and Caliborn all share the same distinctively staggered laugh, and are (respectively) an ultimately malevolent Magnificent Bastard, questionably sentient (yet unquestionably terrifying), and a young version of the main villain.
• A Modest Destiny has a generic female-personality undead skeleton who likes to giggle. She gets recruited by the good guys quickly. Also she thinks her giggle is manly.
• Tower of God: Near the end of the first season, Lo Po Bia Ren, an assassin posing as a magic teacher, tends to giggle a lot at least when showing his true nature. It also seems to occur in conjunction with overexcitement. The guy enjoys his job (of killing people) a bit too much.
  
  The new English translation changes most of the giggling to laughter sounds.

Web Videos

• The Spoony Experiment: Spoony's Dr. Insano character laughs like this. It'd be terrifying if it wasn't so damn funny.
• Dr. Horrible in Dr. Horrible's Sing-Along Blog actively practices his "evil laugh" (with a vocal coach, no less) to avert this and develop something manly-sounding that people will take seriously.
  Dr. Horrible: You think Bad Horse didn't work on his whinny? His terrible death whinny?

Western Animation

• While the Changeling Queen in My Little Pony: Friendship Is Magic can certainly pull off a full blown Evil Laugh, she does giggle creepily when she is amused.

• The Troublesome Trucks from Thomas & Friends giggle and laugh like chipmunks whenever they've caused an engine pulling them to fall into an accident and tease them for their misfortunes. More often than not, the engines respond by telling the trucks to be quiet.
• Chef Hatchet from Total Drama is normally a chuckling villain; however, he has pulled off impressive evil laughs from time to time.
• Transformers:
  • Transformers: Animated:
    • Professor Princess. All the time.
    • Decepticon Blitzwing has three distinct personalities. The Random personality has a fondness for spontaneously giggling that the other two lack.
  • Beast Wars:
    • Tarantulas is rarely heard without his creepy little chuckle.
    • Waspinator qualifies. just not nearly as often.
• In Wakfu, after Qilby the Traitor reveals himself as the Big Bad of season two he becomes very prone to fits of giggling, Slasher Smiles, and mad laughter.
• The villain Smilzo from Buzzatto's West and Soda giggles a lot.
• Robin in Young Justice (2010) plays with this trope. He's a hero, but he unnerves the villains by giggling from the shadows.

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