Voiced by: Rina Sato (Japanese), Greg Ayres (English)
A ten-year-old mage apprentice who gets assigned as the teacher of the 3-A class of Mahora Academy.
- Adaptational Backstory Change: In this version, the darkest parts of his backstory from the original manga such as the destruction of his hometown never happened or at least are never mentioned.
- Baleful Polymorph: Midway through the anime, Negi gets turned into a Chupacabra and is stripped of his magic as punishment for exposing his secret to his students. He turns back to normal after forming provisional Pactios with all his students minus Eva and Chachamaru.
- Cannot Keep a Secret: He's so terrible at keeping his status as a mage a secret that all his students find out halfway through the anime.
- Hates Baths: Like in the manga, Negi hates baths and Asuna has to forcefully wash him.
- It Was a Gift:
Voiced by: Akemi Kanda (Japanese) and Luci Christian (English)
Student No. 08. Negi's feisty roommate and the first to make a Pactio with him.
- Adaptation Personality Change: Asuna goes from being a regular Tsundere, having a job and more or less just trying to survive in the craziness around her in the manga and first anime series, to being a complete Cloudcuckoolander in the Negima!? anime.
- Adaptational Backstory Change:
- Asuna in this anime really is a normal schoolgirl with nothing special in her backstory. This is quite the change from the original manga, where she was an amnesiac magic princess with a mysterious connection to Negi's Disappeared Dad.
- The Negima!? Neo manga does keep the amnesiac magic princess aspect, which is only revealed in the final chapters, but instead of being a human weapon that was rescued by Nagi, Asuna was sent away from the Magic World to protect her from a big war.
- Adaptational Comic Relief: Many of her more serious character traits from the original manga are discarded in favor of a loony girl who is obsessed with the Chupacabra.
- Attention Deficit... Ooh, Shiny!: It's pointed out Asuna has a bad habit to stop paying attention to everything around her whenever she gets some new strange obsession.
- BFS: Her Armor card gives her the same giant sword she uses in the original manga.
- Car Fu: She throws a boat at Evangeline to save Negi from the vampire.
- Cloudcuckoolander: She obsesses over weird things like the Chupacabra and is a total space case in general.
- Club President: She's the founder and president of the Chupacabra club, although she's the only member who's actually in it for the Chupacabra; everyone else is there to learn magic from Negi.
- Cool Big Sis: Despite her wacky personality in this continuity, Asuna acts like a spunky and protective big sister for Negi just like in the manga.
- Epic Fail: She burned down the kitchen when trying to cook.
- Evil Costume Switch: In Episode 23, she gets corrupted by the Darkness after being kissed by a possessed Eva and gets a dark outfit from it.
- Expressive Hair: Her Girlish Pigtails move according to her mood.
- Fashionable Asymmetry: In the outfit of her Armor card, her right leg has a greave while her left leg wears a blue thigh-high stocking.
- Fiery Redhead: Like in the manga, Asuna is a feisty and outspoken Tsundere redhead.
- Full-Boar Action: Her Suka card turns her into a boar, fitting her hotheadedness and aggressiveness.
- Fun T-Shirt: She orders several packages of Chupacabra T-shirts for everyone in her club to wear. She's constantly trying to get people to buy them, but almost everyone finds them of bad taste.
- Heavy Sleeper: In her very introduction, Konoka tries to wake up Asuna to go to school to no avail. Judging by what Setsuna says, it happens often.
- Lethal Chef: Her attempt at cooking ends with her burning down the kitchen.
- Paper Fan of Doom: The equipment of her Cosplay card is a paper fan.
- Peek-a-Bangs: The Darkness-controlled Asuna from Episode 23 has her left eye covered by her bangs.
- Really Royalty Reveal: In the Negima!? Neo manga, she's revealed to be a princess of the magic kingdom of Ostia who had her memory wiped and was sent to Mahora for her own protection during a war.
- Scarf of Asskicking: Her Armor card gives her a white scarf as well as a BFS to fight.
- Scholarship Student: Konoka mentions Asuna is a student because of a scholarship.
- Sent Into Hiding: In the Negima!? Neo, Asuna is a princess of a magic kingdom who had her memory erased and was sent to Mahora Academy for her protection because of a war in the Magic World.
- Tsundere: It gets downplayed compared to her manga counterpart due to being more of a Cloudcuckoolander here, but it's still there. Asuna is generally brash, hotheaded, violent and a Cool Big Sis who can be very nice to Negi on occasion.
- Zettai Ryouiki: Her Armor card gives her a Grade S; short skirt, legs covered up to the thighs, Girlish Pigtails and a Tsundere.
Voiced by: Mamiko Noto (Japanese) and Leah Clark (English)
Student No. 27. A Cute Bookworm who soon develops a crush on Negi.
Voiced by: Ai Nonaka (Japanese) and Monica Rial (English)
Student No. 13. The bubbly roommate of Negi and Asuna.
- Bifauxnen: In the Negima!? Neo manga, Konoka puts on a Shinsengumi uniform while Setsuna is wearing a luxurious kimono when they visit Kyoto's Cinema Village, which is the reverse of the same situation in the original manga.
- The Ditz: She'll try and do a form of fortune telling known as tortoiseshell readings... with eggs. When the first egg explodes, she thinks she just has to try again with more eggs.
- I Didn't Mean to Turn You On: Konoka's attempts to cheer Asuna up during the Beach Episode OVA ends up with her unwittingly waving her butt in Setsuna's face, which nearly gives her a heart attack.
- Improbable Weapon User: The weapon she gets from her Armor card is a set of expanding fortune-telling chopsticks, which she is able to use like a whip.
- Making a Splash: With her Cosplay card, she is able to spout water from the fans that come with her costume.
- Male Gaze: In the Beach Episode, the camera and Setsuna like to put a lot of focus on Konoka's butt and breasts when she's wearing a swimsuit.
- Schoolgirl Lesbians: Like in the manga, she has an obvious Bodyguard Crush relationship with Setsuna and they get a Ship Tease moment at least Once per Episode.
- Smells Sexy: Setsuna likes the scent of her hair.
- The Tease: She likes to tease Setsuna by doing things like inviting her to take a bath together just to get flustered reactions out of her.
Voiced by: Yuu Kobayashi (Japanese), Cherami Leigh (English)
Student No. 15. The personal bodyguard and childhood friend of Konoka.
- Adaptation Species Change: In the original manga, Setsuna is a half-crow demon girl who was born with white wings instead of black. In the Negima!? anime, she's completely human and only gets wings from her Armor card.
- Adaptational Backstory Change: In the original manga, Setsuna is a member of crow demon tribe who was exiled for being a half-breed albino. In the Negima!? anime, she's a regular human who is descended of a family of servants for the Konoe family.
- Affectionate Nickname: Secchan, from Konoka.
- Bodyguard Crush: She has an obvious crush on her charge, Konoka.
- Character Exaggeration: Setsuna becomes little more than a Covert Pervert Token Yuri Girl in the Negima!? continuity while in the manga she had a wider characterization even if her huge Bodyguard Crush on Konoka was a defining trait to her character.
- Childhood Friend Romance: She was playmates with Konoka for a short time when they were children. However, Setsuna thinks it's improper to let their past friendship get in the way of her job as a bodyguard.
- The Comically Serious: Most of the humor involving her is about Setsuna taking everything way too seriously and coming off as pretty silly.
- Covert Pervert: She's very serious and formal most of the time, always treating Konoka with utmost respect. When Konoka is not paying attention, Setsuna does things like smelling her hair and ogling at her butt while she's wearing a swimsuit.
- I'm Taking Her Home with Me!: In the final episode, Setsuna is carrying Konoka in her super-cute Suka form. As Suka Konoka repeatedly comments on how comfortable she is in her arms, Setsuna comments that she wishes she could "take her home like this." Setsuna is so horrified at her "improper" thoughts that she proceeds to beg Suka Konoka to not let her hold her again.
- Legacy of Service: The Sakurazaki family have been servants and protectors of the Konoe family for generations.
- Power Gives You Wings: Her Armor card gives her feathered wings.
- Schoolgirl Lesbians: Her blatant Bodyguard Crush on Konoka gets teased at even more often than in the manga and their relationship is the subject of several eyecatchers.
Voiced by: Natsuko Kuwatani (Japanese) and Brina Palencia (English)
Student No. 04. Nodoka's best friend.
- Demoted to Extra: She's nowhere near as significant a character in this anime as she is in the manga.
- Dull Surprise: In Negima!?, in one of the early omake segments with the Baka Rangers, while the Bakas make ready to fight their enemies, with Yue wanting to call their "giant robot", Makie is complaining that she never got to do her introduction properly. The Baka Rangers agree to let her go first (she had been last every time). She tries to do so, and the giant robot foot comes down on Makie, interrupting her.
- Elegant Gothic Lolita: Her Cosplay costume is a Victorian-style mini dress full of lace and frills.
- Matchmaker Crush: Similar to the original manga, Yue has a crush on Negi even though she's supposed to be supporting her friend Nodoka's feelings for him. Yue decides to suppress her feelings for Nodoka's sake, and never directly confesses her feelings to him.
- The Owl-Knowing One: To reflect her intellectual character, Yue transforms into an owl as her Suka form.
Voiced by: Yuri Shiratori (Japanese) and Kate Bristol (English)
Student No. 01. The resident ghost of the class.
- Angelic Beauty: Her Armor Card depicts her with feathered wings and a halo, making her look like a cute angel.
- Cute Ghost Girl: Such an adorable ghost that she's the trope picture.
- Friendly Ghost: While she's a ghost, she is very nice and never tries to scare anyone, at least not intentionally.
- Ghost Amnesia: She has no memories of when she was alive and doesn't know how she died.
- Invisible to Normals: At first, no one in the class is able to see her. Negi learns a spell so she becomes visible to him and Eva casts a spell so the other girls can see Sayo through Kazumi's camera. After she makes a Pactio with Negi, this somehow makes her visible to everyone.
Voiced by: Madoka Kimura (Japanese), Amber Cotton (English)
Student No. 02.
Voiced by: Ayana Sasagawa (Japanese) and Monica Rial (English)
Student No. 03.
Voiced by: Kotomi Yamakawa (Japanese) and Avery Rice-Williams (English)
Student No. 05.
- The Medic: She acts as the personal nurse of the class, healing others and coming up with strange medicines and needles to help them out.
Voiced by: Azumi Asakura (Japanese) and Cherami Leigh (English)
Student No. 06.
- Dull Surprise: In the Negima!? Neo manga, Negi accidentally lets slip that he's a mage in front of Akira. Akira swears that she's shocked by Negi's secret, but the manga itself points out that she isn't the type whose emotions show in her face.
- Pet Baby Wild Animal: Her Day in the Limelight chapter in the Negima!? Neo manga has Akira finding a sick baby dolphin she names Ruka. She takes care of it until it fully recovers and she releases it back into the ocean.
Voiced by: Ai Bandou (Japanese) and Wendy Powell (English)
Student No. 09.
Voiced by: Akeno Watanabe (Japanese) and Caitlin Glass (English)
Student No. 10. The robot servant of Evangeline created by Hakase.
- Character Exaggeration: Her Emotionless Girl behavior is taken to the extreme here.
- The Comically Serious: She can be almost as wacky as her classmates while keeping her expressionless face.
- Curtains Match the Window: Her hair and eyes are light green.
- Emotionless Girl: She never expresses emotions and speaks in a monotone voice.
- Morality Pet: Evangeline showing to care for Chachamaru is one of the first things that proves she's not really evil.
- Robot Buddy: She's a robot who serves as Evangeline's personal assistant and combat partner.
- Robot Girl: She looks like a normal girl except for the antennae she has in place of ears being proof that she's a robot.
- You Gotta Have Blue Hair: Pale green hair, in her case.
Voiced by: Hazuki Tanaka (Japanese) and Cynthia Cranz (English)
Student No. 12.
Voiced by: Sawa Ishige (Japanese) and Jamie Marchi (English)
Student No. 14.
Voiced by: Yui Horie (Japanese) and Kate Oxley (English)
Student No. 16.
- Butt-Monkey: She gets a Color Failure from being called a failure by Ayaka at least Once per Episode.
- The Ditz: She's one big airhead and Ayaka always berates how dumb she is.
- Everything's Better with Monkeys: Her Suka form is a monkey.
- Failure Is the Only Option: Ayaka calls her a failure for every mistake and silly thing she does. In the penultimate episode, Makie is being competent for once while helping prepare a party. Ayaka calls her a failure anyway.
- Running Gag: She calls Motsu the frog "dad" and he has no idea why, but eventually warms up to the idea of Makie being his daughter. In the final episode, Makie finally explains why she calls Motsu that; she once had a pet dog who looked like Motsu and that dog was called Dad.
Voiced by: Miho Sakuma (Japanese) and Stephanie Young (English)
Student No. 18.
- Gun Nut: Mana is almost never seen without a gun or rifle in her hands.
Voiced by: Megumi Takamoto (Japanese) and Trina Nishimura (English)
Student No. 19.
- Bare Your Midriff: Her Armor outfit comes with a somewhat short shirt that lets other see her navel.
- Combat Hand Fan: Her Armor card transforms her into a martial arts warrior with a metal tessen.
- Panda-ing to the Audience: Her Suka form is a cute panda, going along her Anime Chinese Girl gimmick.
- Unrelated in the Adaptation: She isn't Negi's descendant from the future in this continuity.
- Zettai Ryouiki: Her Armor outfit includes a miniskirt and thigh-high socks, leaving a bit of her leg skin bare.
Voiced by: Ryoko Shiraishi (Japanese), Clarine Harp (English)
Student No. 20.
- Heroic BSoD: Played for Laughs in the final episode. Kaede goes into a state of shock after the Narutaki twins take a look at how she'll look in the future and their reactions suggest it won't be good at all.
- Kappa: Her Suka form is a kappa.
- Tengu: The costume of her Cosplay Card looks like that of a traditional tengu, including the long-nosed red mask and leaf fan.
Voiced by: Misa Kobayashi (Japanese) and Amy Rosenthal (English)
Student No. 21.
Fuuka is voiced by: Kimiko Koyama (Japanese) and Alison Viktorin (English)
Fumika is voiced by: Mari Kanou (Japanese) and Alison Viktorin (English)
Students No. 22 and No. 23.
- Ascended Extra: The Narutaki twins never actually join Negi's group in the manga, but here they do.
- Identical Twin ID Tag: They look identical except that Fuuka has pink eyes and Girlish Pigtails while Fumika has light blue eyes and Odango Hair.
- Kid Detective: Their Cosplay costumes are both of detectives, except Fuuka's is Japanese style and Fumika's is Western style.
- Older Than They Look: While they look like children, they're fifteen years old like their classmates.
- Rose-Haired Sweetie: The two have pink hair and have very cheerful personalities.
- Single-Minded Twins: They almost always speak the same lines, sometimes in in unison.
- You Gotta Have Blue Hair: Both have pink hair.
Voiced by: Mai Kadowaki (Japanese) and Carrie Savage (English)
Student No. 24.
- Bag of Holding: The costume of her Cosplay Card comes with a magic bag where she can pull out the pieces to construct whatever she wants. In the final episode, she uses it to build a satellite.
Voiced by: Yumi Shimura (Japanese) and Caitlin Glass (English)
Student No. 25. A grumpy girl with the secret hobby of being a net idol.
- Butt-Monkey: She often gets teased by her classmates about her not-so-secret identity as a net idol. The other girls also tend to steal her online popularity without even trying.
- Demoted to Extra: Chisame's prominence is greatly diminished in this adaptation.
- Embarrassing Nickname: Some of her classmates start calling her "Chiu-Chiu" after her net idol hobby gets exposed. Chisame is extremely annoyed by this.
- Grumpy Bear: She's the one to always be in a cranky mood in a class full of quirky and cheerful girls.
- Open Secret: After a while, about everyone in her class knows about her net idol hobby. Chisame still tries to deny it to no avail.
- Rose-Haired Sweetie: As Chiu, her hair turns light pink and she drops her grumpy attitude to become a Kawaiiko.
- You Gotta Have Blue Hair: Her hair is light pink when she's Chiu.
Voiced by: Yuki Matsuoka (Japanese) and Laura Bailey (English)
Student No. 26. A vampire sorceress who was trapped inside Mahora Academy 15 years before by Negi's father.
- Adaptation Expansion: The Negima!? Neo manga significantly expands on Evangeline's backstory with Negi's father, Nagi. Two whole chapters are dedicated to explaining in detail how Evangeline met Nagi and why she developed a crush on him. The flashback adds interactions between her and Nagi that weren't in the original manga, most notably the two working together to save a human village from a horde of evil demons.
- Aloof Ally: After she gives up on fighting Negi, she keeps showing up to help him and his students when the Darkness causes trouble. She still tries to act like she doesn't care, of course.
- Attack of the 50-Foot Whatever: In Episode 20, the Darkness turns Eva into a giant.
- Bunnies for Cuteness: Her Suka form is a cute black bunny.
- Butt-Monkey: Despite trying her damnedest to be threatening, she gets beaten in an undignified way by both Asuna and Negi. From then on, she's one of the most frequent victims of slapstick.
- Demonic Possession: After all the shards of the Star Crystal are gathered, Eva is possessed by Anya's Darkness and attempts to turn everyone in Negi's class, Takamichi and Nekane into stone.
- Elegant Gothic Lolita: Her Cosplay costume is an extremely cute Sweet Lolita style dress that makes her look even more like a doll. Funnily, this attire comes at odds with her grumpy personality.
- HeelFace Turn: Similar to the original manga, she's introduced as Negi's enemy. Once Negi beats her, she turns into an Aloof Ally.
- Insult Backfire: In Chapter 25 of the Negima!? Neo manga, Evangeline shows Negi a projection of her past with Nagi. The first thing she shows Negi is her annihilating a nation's army by herself. Negi calls this cruel and inhuman. In response, Evangeline is pleased that Negi is impressed by her true power. Negi reproaches that he wasn't complimenting her.
- Jerk with a Heart of Gold: While she acts rude and apathetic, she does care about Chachamaru and always goes out of her way to help Negi and her classmates at times of trouble.
- Our Vampires Are Different: She was turned into a vampire at ten years old, leaving her stuck at that age. She gains more power by drinking blood and is at her strongest on nights of full moon.
- Pet the Dog: She makes Cute Ghost Girl Sayo visible to her classmates for no real reason other than trying to be nice for a change.
- Sarcastic Clapping: Does this in response to Negi casting a spell to see spirits, but he doesn't realize she's being sarcastic.
- Sour Outside, Sad Inside: While she acts aloof and moody, she's distressed to the point of tears as she expresses her unbearable frustration with being an unaging child imprisoned against her will at a school.
- Starter Villain: Similar to the original manga, she initially antagonizes Negi so she can drink his blood and break free of the curse from his father. After being defeated, she becomes an Aloof Ally to the class.
Voiced by: Mai Aizawa (Japanese) and Majken Bullard (English)
Student No. 28.
- Bare Your Midriff: The Pirate Girl outfit of her Cosplay card comes with a midriff-baring top.
- Butt-Monkey: About all her appearances have her as the victim of Chizuru's Trolling.
- Demoted to Extra: A minor variant. Since Kotaro was Adapted Out, Natsumi's role is simply to be teased by Chizuru.
- You Gotta Have Blue Hair: She has dark green hair.
Voiced by: Junko Minagawa (Japanese) and Laura Bailey (English)
Student No. 29. The class representative who harbors a disturbing obsession with Negi.
- Bare Your Midriff: Her Cosplay dress has an opening at the front that shows off her navel.
- Character Exaggeration: Her infatuation with Negi is taken to ridiculous degrees in Negima!?.
- Childhood Friends: Like in the manga, she has known Asuna since elementary school and despite their fights, they're obviously good friends.
- Comedic Shotacon: An even more blatant one than in the manga. Her obsession with Negi goes all the way to keeping all sort of merchandise with his face in it.
- Crazy-Prepared: In Negima!? Neo, during a kiss Negi competition, she and most of the other girls get caught on a bridge that collapses. Four pages later, she re-enters the story with the other girls caught up in a net, wearing a bridal dress and doing her trademark Noblewoman's Laugh."I thought this might happen. So, I had them prepare the Yukihiro Conglomerate's private helicopter!"
- Dressed Like a Dominatrix: The attire provided by her Armor card is a black Minidress of Power with a corset, high-heeled thigh-high boots, opera gloves, and a fluffy choker. The outfit comes equipped with a whip.
- Stalker with a Crush: Her obsession with Negi easily crosses over into creepy territory. Her entire room is filled with Negi-themed merchandise including posters, pillows, swimsuits, an alarm clock which plays Negi's voice and bread.
- Whip It Good: The weapon provided by her Armor card is a whip.
- Zettai Ryouiki: Her Armor card gives her a black Minidress of Power and thigh-high black boots.
Voiced by: Naomi Inoue (Japanese) and Monica Rial (English)
Student No. 30.
Voiced by: Yuka Inokuchi (Japanese) and Carrie Savage (English)
Student No. 31.
Misa is voiced by: Shizuka Ito (Japanese) and Gwendolyn Lau (English)
Sakurako is voiced by: Akane Omae (Japanese) and Colleen Clinkenbeard (English)
Madoka is voiced by: Mami Deguchi (Japanese) and Mary Morgan (English)
Students No. 07, No. 11 and No. 17. The cheerleader trio of the class.
Voiced by: Masashi Yabe (Japanese) and Chris Cason (English)
Negi's talking ermine companion.
Voiced by: Chiwa Saito (Japanese) and Brittney Karbowski (English)
Negi's childhood friend.
- Adaptational Nice Guy: While Anya in the Negima!? anime is still brash and competitive, she's much less irritable and violent compared to the Tsundere Bratty Half-Pint from the original manga. The worst she does to Negi when she's not controlled by the Star Crystal is tell him there's no way he can handle being a teacher in Japan.
- Adaptational Villainy: Anya is revealed to be the Big Bag in this anime adaptation.
- Ascended Extra: Anya has a much bigger role in the Negima!? anime than in the original manga, and an even bigger one in the Negima!? Neo manga, where she becomes the 32nd member of Class 3-A and Negi's third roommate come Chapter 15.
- Big Bad: She's the Master of Darkness who stole the Star Crustal and has been attacking Negi and his class. Although, she was possessed by the Star Crystal and Negi knows the real Anya would never try to make him suffer.
- Demonic Possession: When she tried to steal the Star Crystal, she got trapped inside it and the artifact used her negative emotions to control her and have her attack Negi's class.
- Driven by Envy: She stole the Star Crystal because she felt jealous that Negi's assignment was going so well in Japan while she was both unsuccessful as a Fortune Teller and lonely. Under the influence of the dangerous magic artifact, she targets Negi's class so he ends up alone like her.
- Fortune Teller: Her job assignment was to be a fortune teller in London. She wasn't successful at all, which is one of the reasons she stole the Star Crystal. Negi is able to figure out Anya is behind everything when he learns the students who were possessed were connected to Anya's knowledge of Lunar Astrology.
- Hates Being Alone: The people of the stage she was staying in were usually out and Anya was left all alone. Feeling sad and lonely, she took it very badly when Nekane told her Negi was getting along with his students.
- Heart Beat-Down: She throws offensive spells that are shaped like hearts.
- Ninja: In the Negima!? Neo manga, she decides to take ninja lessons from Kaede.
- Sore Loser: She absolutely hates the idea of losing to Negi in any way.
- Unknown Rival: She's pretty competitive towards Negi and makes it her personal goal to win against him. Although, Negi has no idea of this and treats Anya as his friend instead of a rival.
Voiced by: Miyuki Sawashiro (Japanese) and Gwendolyn Lau (English)
Negi's older cousin and sister figure.
- Ascended Extra: Like Anya, Nekane plays a more prominent role in the Negima!? anime than in the original manga.
- Big Sister Instinct: She traveled all the way from England to Japan so she could look after Negi in disguise and attempt to save Anya from the control of the Star Crystal.
- Cloudcuckoolander: After arriving at Mahora, Nekane comes off as a bit of a wacko and does random things that weird Negi's students out. When some of the girls question why Nekane is acting so strange, Negi states that's just the way she always is.
- Cool Big Sis: She's a loving big sister figure who is adored by both Negi and Anya.
- The Ditz: She acts very spacey and clueless when she shows herself to Negi's class.
- Hair of Gold, Heart of Gold: A cheerful, kindhearted and motherly young blonde woman.
- Identical Stranger: She's basically a blonde and gentler Asuna.
- It's All My Fault: She blames herself for Anya getting possessed by the Star Crystal because it all started when she carelessly told Anya how well everything was going for Negi.
- Naked First Impression: With Asuna and Konoka, who first find her in the bath.
- Open Secret: After a while, everyone in the class figures out Nekane is the Black Rose Baron. Since Nekane still thinks it's a secret, they all play along.
- Parental Substitute: Since their parents are nowhere to be seen, Nekane is the closest thing to a mother Negi and Anya have.
- Sweet Polly Oliver: She disguises herself as the Black Rose Baron to covertly help Negi fight off the Star Crystal's Darkness.
- Unwitting Instigator of Doom: She indirectly caused the whole mess with the Star Crystal. She carelessly told Anya that Negi's job as a teacher was going well and he had made friends with his students, which didn't sit well for Anya when her job as a fortune teller went badly. Anya's jealousy and desperation pushed her into stealing the Star Crystal and she targeted Negi and his class.
Voiced by: Norihiro Inoue (Japanese), Mark Stoddard (English)
A teacher of Mahora Academy who knows Negi from before he arrived.
- Adaptational Superpower Change: Unlike his manga counterpart, Takahata does have magical abilities in this series and never uses Kanka.
- Cordon Bleugh Chef: Takahata attempts to make "World Delicacy Noodles" by combining foods from all around the world into one bowl of ramen. It's very effective at rendering other characters unconscious.
- Lethal Chef: His "World Delicacy Noodles" can give food poisoning to even an ancient vampire.
- Promoted to Love Interest: Inverted. At the start of the Negima!? anime, it seems Asuna has a crush on Takamichi like she does in the original manga, but she gets over it after she becomes obsessed with the Chupacabra and other weird things.
- Trademark Favorite Food: He's almost always sipping on ramen.
Shichimi is voiced by: Miyuki Sawashiro (Japanese) and Brina Palencia (English)
Motsu is voiced by: Chiwa Saito (Japanese) and Trina Nishimura (English)
A pair of "envoys" from the Magic World. Shichimi is a cat and Motsu is a frog. They were originally sent to inform everyone about the Star Crystal disappearance. Haruna essentially "created" their forms. After they provide the news, they're mostly there to keep an eye on Negi and his magic, then once all the girls have a pactio, to merely just be annoying.
- Black Comedy Cannibalism: In the penultimate episode, Motsu drinks Eva's skin potion which includes frog as one of its ingredients, to his horror.
- Canon Foreigner: These guys only appear in the anime Negima!?.
- Catchphrase: Motsu's "...in a good way".
- Familiar: Their true identities are the servants of Nagi Springfield who were sent to keep an eye on his son. In the final episode, Nagi leaves them with Negi to be his new servants.
- Those Two Guys: They're always seen together and don't do much aside from making some jokes here and there.
- Verbal Tic: Shichimi adds "Mya" to the end of her sentences. This is carried over into the English dub.
Voiced by: Miyuki Sawashiro (Japanese) and J. Michael Tatum (English)
The famed Thousand Master and Negi's long-lost father.
- Big Damn Heroes: He shows up in the climax to save Negi and Anya from being absorbed by the Star Crystal.
- Disappeared Dad: Like in the original manga, Nagi went missing when Negi was a very young child and Negi hopes to find him one day.
- Red Herring: Negi suspects his father is the true identity of the Black Rose Baron, possibly being controlled by the Star Crystal. It turns out the Black Rose Baron is Negi's cousin Nekane playing Mysterious Protector.
- Vocal Dissonance: Unlike the other anime adaptation, Nagi is voiced by a woman in Negima!?. As a result, he sounds more like a young boy instead of an adult.
Voiced by: Miyuki Sawashiro (Japanese) and Gwendolyn Lau (English)
A mysterious man who occasionally shows up to help out Negi's group and give them advice. Negi has a feeling that he knows him, causing him to suspect he's his missing father.
- Large Ham: His theatrical costume and affinition for dramatic entrances say it all.
- Mysterious Protector: He randomly appears and disappears whenever Negi's group is in trouble. Negi wonders if he could be his father protecting him. The Baron is really Nekane who is trying to protect Negi from the Darkness while also trying to save Anya from the Star Crystal.
- Samus Is a Girl: The Baron's true identity is Nekane Springfield, Negi's female cousin who came to Japan to help him deal with the Star Crystal.
Shows up in the final chapters of the Negima!? Neo manga. Fate is a prince of the magic kingdom of Ostia who arrives at Mahora Academy to ask Asuna to marry him. Needless to say, he has nothing in common with the original manga's Fate aside from the name and character design.
- Adaptational Nice Guy: Unlike Manga!Fate, this Fate is not a stoic Jerkass and never attacks Negi or his students. Instead, he's a Prince Charming who truly wants to make his princess Asuna happy and when he realizes Asuna wants to stay with Negi, he peacefully backs down.
- Childhood Friend Romance: Prince Fate was friends with Asuna when they were children and he comes to Mahora to ask her to marry him even though she had her memories of him erased.
- Forgotten First Meeting: He was Childhood Friends with Asuna when they both lived in Ostia. She can't remember him because her memory was erased before being sent to the normal world to protect her from a war.
- I Want My Beloved to Be Happy: He gives up on making Asuna his wife after he understands what she really wants is to stay in Mahora with Negi.
- Literal Genie: He grants Asuna's wish of being the smartest person in Mahora by making everyone else into an idiot. When she asks for infinite treasures, he gives her a massive diamond ring impossible to wear and turns her classmates' uniforms and Setsuna's sword into gems.
- Prince Charming: A very stereotypical one, being a handsome prince of a magic land and appearing to make a princess his bride.
- Romantic False Lead: He shows up at the end of the Negima!? Neo manga with the intention of taking Asuna away from Mahora Academy so they can marry in the Magic World. Of course, it all ends with Asuna choosing to stay with Negi.