Anti-Hero: In the Cardinal Route, she is a mild example. While she is responsible for many deaths, those were in self-defense, and ultimately she created a better world for everyone by destroying the twelve Zodiac Crowns and therefore hierarchy. She is also willing to pay for the damage that she caused during her quest.
God Save Us from the Queen!: Virgo is the only Zodiac who has the title of "Queen," and as evidenced by the fact she is often referred to as "Dreadful Queen" by "heretics," she is not exactly well-liked. Nor is she as pure as she believes she is. The denizens of the Holy Asylum see her as The High Queen, though.
Muscles Are Meaningless: Zig-zagged. She's apparently jacked in-universe (even if her character art presents her as very thin) and is a competent fighter, but the other members of her party are strong too despite their lack of muscles, and so are the rest of the Zodiacs (some of which are technically stronger than her).
Virgo: Will you, really? Someone like you... Could just drop dead and nobody would notice. Don't you think so? Cancer: What you mean... someone like me? Virgo: What do I mean by that...? I'll tell you what I mean. Although you breathe and you are living, well... I wonder what hope there could possibly be. You're someone... Who is so spineless that people can treat them whatever they want! Being used by Capricorn, having all of your profits go to her corporation... And still crawling here like this, licking her boot to gain some approval from that filthy, greedy Zodiac. You call this revenge, really? You are merely a cursed child... Cursed to live forever, without your own children for your own realm. No organic denizens. Cancer: (Stunned Silence) Virgo: Hah... And you're so alone you even spy on the other Zodiacs... For what, the company? Your existence isn't even useful! You just keep living your useless life, since you were thrown out in the world without any kind of blessings. If you were more courageous, I expect you would have disappeared a long time ago. You're pitiful. Did you know that? I pity you so much that I feel the need to do what you can't do yourself. I'll make you disappear.
Red Baron: Virgo, the Holy Queen. Queen of Purity and Dreadful Queen also qualify.
Sanity Slippage: Virgo goes off the deep end in the Fixed Route, becoming a violent, easily angered, self-important mess who is willing to sacrifice Ginger and kill her own companions if they don't follow her orders. She even refuses to believe Pisces is dead, going around the galaxy in a vain attempt to find her.
Tsundere: Virgo is this towards her girlfriend Pisces, and even her friends Algol and Spica. This is acknowledged in-universe, with Spica saying Virgo is a Tsundere when he is properly introduced, and Algol teasing Virgo about it.
Hopeless Suitor: Seems to be into Virgo, but she doesn't even like him to begin with and is already dating Pisces. It doesn't matter too much, since later on, he goes for Algol instead. And Algol apparently likes him back.
Spica: I followed your cause because I believed in you. But now... You don't seem like the Holy Queen I once knew. The Queen who created your Realm and me. Your voice has changed, My Queen, and I don't find myself compelled to follow it any longer.
Algol (after Virgo kills Spica): By the damned rulers, you really killed him... You think you deserve the crown, Virgo? You think you deserve the Golden Age? May my spells curse you for eternity... That's what you REALLY deserve!
Everyone Has Standards: In the Fixed Route, Algol is disgusted by Virgo's willingness to sacrifice Ginger to get the Golden Crown, rightfully considering her to be insane. When Virgo does nothing to help her own case, she turns against her, along with Spica.
Freudian Excuse: Became the way she is today after Perseus betrayed her trust and killed Medusa, her mother. She admits she used to be innocent back then, but hasn't felt anything since.
The Gadfly: Yup. Particularly notable is when she teases Virgo about her Tsundere tendencies.
Hero Antagonist: She turns against Virgo, along with Spica, in the Fixed Route.
Lack of Empathy: She outright admits that she doesn't care about the stars that she's killed.
Algol: Awwwwwwwwwww man, that's adorable! I think I'm feeling feelings! I'm not sure if I like them.
That being said, Algol is not nearly as bad as she seems to be at surface level. She actually values Virgo and Spica as her friends, and she'll fight anyone who is willing to sell out a friend, as shown in the Fixed Route.
Meaningful Name: Algol is the name of a star that's colloquially known as the Demon Star, and it is associated with violence across a wide variety of cultures. Although Algol is not located in the constellation of Taurus, it is right next to it, hence why she is a Tauresean in the game. Its actual location in the sky, however? The constellation of Perseus.
Pay Evil unto Evil: What Algol wants to do, ultimately. She wants to bring back Perseus, the murderer of her mother, just so that she can kill him.
Revenge: Algol's motivation. Perseus used her to get closer to her mother Medusa, wanting the fame that would come with Medusa's head. Perseus did manage to kill Medusa, but Algol caught him in the act, immediately removing her mask and turning him to stone in retaliation. However, Algol soon came to believe that petrification was too merciful of a fate for Perseus, so she started gathering Star Cores to remove the curse she had put on him, all in the hopes of killing him with her own hands.
Unscrupulous Hero: Despite being a murderer who feels no guilt for her actions, Algol is really not that bad, helping Virgo even after her own quest is finished. In fact, she actually turns against Virgo in the Fixed Route. Maybe Pisces' decision to free Algol from the Astrological Prison wasn't so wrong, after all.
Used to Be a Sweet Kid: According to herself, she used to be much more innocent. Perseus changed this, though she's still not all that bad underneath.
Always Female: Eleven of the Zodiacs are female. The last one, Aries, is non-binary.
Animal Motifs: Goats for Capricorn, bulls and cows for Taurus, crabs for Cancer, rams for Aries, lions for Leo, scorpions for Scorpio, fishes for Pisces and horses/centaurs for Sagittarius.
Cast Full of Gay: Several of them are lesbian. Taurus, Scorpio, Virgo and Pisces are prominent examples.
Ambition Is Evil: Capricorn is a cardinal sign, and those are associated with ambition. However, she takes it too far, turning her realm into a rigid work environment and generally not caring about anything other than profit.
Animal Motifs: Goats. Almost all of the denizens in her realm are goats (or lambs) and the horns in her crown look like those of a goat's.
Bad Boss: Doesn't seem to care about the Manifesto's demands of better work conditions.
Capitalism Is Bad: The system within her realm is called "Capritalism" and is defined by overworking her employees and employing children, among other things, to the extent that a rebellion is taking place when Virgo arrives to take her crown. Capricorn herself is a cold-hearted woman motivated by greed, who Virgo calls out for only caring about her own daughter's life for logistical reasons.
Lady of War: Capricorn fights rather gracefully, and she stays in the proper sitting position during the entire battle. Sure enough, she is often referred to as "Lady Capricorn."
Leitmotif: "The Time Keeper," a pretty rocking theme that makes the fight against her all the more awesome. Additionally, "Gateways" is played when Virgo is in her presence, and it sounds pretty imposing, fitting her serious, no-nonsense demeanor.
No-Sell: In the later half of the battle, the saboteurs are unable to deal damage to her with their cherry bombs. In contrast, she kills both of them in one hit.
Not So Stoic: She is enraged by the idea of bowing down to the rulers once more. Also, after she is defeated, she expresses sadness.
Capricorn: I feel sadness, Virgo, Queen of Purity. But this lotus dries my tears.
Pet the Dog: In the Cardinal Route, Capricorn will apologize for misjudging Virgo after discovering that she wasn't responsible for Deneb Algedi's murder. Furthermore, she also tells Virgo and everyone else that somebody intends to set the Seamitery Lighthouse on fire so it is best to evacuate it, helps Virgo defeat the viruses and informs her of a method to cure Pisces. This is despite the fact that Virgo wrecked her company and took her crown.
Time-Limit Boss: If Virgo doesn't defeat her in ten minutes, her meeting with Capricorn will end. Furthermore, during the later half of the fight, Capricorn speeds up time, thus rigging the battle in her favor.
Time Master: Capricorn lives up to her title by being able to strike faster than time, as well as being able to speed it up. She is also a Time-Limit Boss, as stated above.
Western Zodiac: Like the sign she personifies, she is cold, ambitious and hard-working. However, she's a villainous takeon the concept. In addition, she is a cardinal sign with earth powers who used to be ruled over by Saturn, and is associated with goats.
Worf Had the Flu: While she's not an easy boss by any means, she's much stronger when she helps you fight against the corrupted Pisces and the viruses in the Cardinal Route. This is because the lotus in her eye had a virus in it that drained her energy, and by the time she fights by your side, Pisces has already removed this virus for her.
Ax-Crazy: Scorpio is introduced attacking one of the denizens of The Sacred Forest for sleeping instead of attending choir practice for the Food Festival, and once attempted to kill Virgo in her sleep.
Resurrective Immortality: As per her title, she revives every time her HP falls to zero. She can also revive others, which she does to Taurus' denizens who were sacrificed. She has her limits, however.
Nice Girl: Sagittarius is nice, silly and generally likeable. She only fights with Virgo because she believes Virgo's objective is to bring back the rulers' strict government, and as she values freedom above all else, she cannot allow that.
The Philosopher: It's her title, and though she is far from a typical portrayal of one, she often talks about friendship, destiny and freedom.
Playing with Fire: Which is reflected in one of her nicknames, The Fire Moth. It makes sense, since Sagittarius is a fire sign.
Recurring Boss: Sagittarius is the only Zodiac that's fought twice in every route.
Red Baron: Sagittarius, the Philosopher. Also, The Fire Moth.
Western Zodiac: Like the sign she represents, Sagittarius is wise and freedom-loving. In addition, she is a mutable sign belonging to the element of fire and associated with centaurs, and she even fights with a bow.
We Used to Be Friends: Tells this to Virgo after she is defeated for the second time, as she and Virgo used to associate, with Sagittarius even providing useful advice regarding Pisces, her sister. Virgo herself has trouble remembering when her relationship with Sagittarius fell apart.
Animal Motifs: Bulls. Not only are those who live at The Sacred Forest usually bulls or cows, but the horns in her head ressemble those of a bull's and her attacks include charging at Virgo.
Berserk Button: Okay, "berserk" might be an exaggeration, but if you choose to be merciful after defeating her, she'll be offended by how you hold no respect for the duel, and will call you a coward before fighting you again.
Fatal Flaw: Her stubborness, which she acknowledges shortly before her death.
Hikikomori: Hasn't left the treehouse for a very long time.
Idle Rich: Despite being the ruler of The Sacred Forest, she prefers to sleep and eat over most other things, including doing anything for her realm. She makes no effort to change The Sacred Forest's harmful traditions of sacrifice and idolatry towards her, to the point that Virgo's intervention is necessary for any change at all to happen.
Killed Off for Real: If you choose to take mercy on her after winning the fight against her, it'll lead to another battle, this time to the death. Killing Taurus results in her realm being destroyed too, since it is linked to her.
Last Words: "I shall close my eyes for one last dream."
Serial Romeo: Possibly as a reference to Taurus being considered a sensual sign, she is defined by her relationship with Scorpio and is known to have a history with her priestess Aldebaran, the brightest star in the constellation of Taurus. The other Zodiacs tend to have a single love interest.
Sleepyhead: Hence her title. There are few things she likes to do more than sleeping.
Dumb Muscle: In Virgo's own words, Aries is too idiotic to win a fight, despite being the toughest Zodiac.
Final Boss: In the Cardinal Route. They are against Virgo's plans of destroying the twelve Zodiac crowns because they want to keep the Krios Volcano, the source of their power, "nice and hot," while being unaware that, if they kept the sacrifices going, eventually the Krios would engulf the Vampire Hotel.
Gender-Neutral Writing: Aries is referred to with they/them pronouns, signifying their non-binary gender identity.
Hard Work Hardly Works: Spent years training to beat Virgo, but was still unable to defeat her in combat. Justified, since they forgot to train their brain, and Virgo also trained a lot prior to the battle.
Hypocritical Humor: Tends to call Virgo "dumb" and "moron," despite being the dumbest of the Zodiacs.
The Rival: They're dedicated to defeating Virgo to the point of training for years to achieve that goal. Whenever they come across Virgo, they make odd threats and attempt to get in a fight with her. This is pretty one-sided, since Virgo considers them a mere insufferable child.
Happiness Is Mandatory: When you enter the Fortress of Dolls for the first time, you can answer a survey if you talk to a certain bear. The last question is "Are you happy living in the marvelous Fortress of Dolls?" with all of the answers being "Yes." Furthermore, a sign at the entrance says "Welcome to the Fortress of Dolls, where unhappiness doesn't exist." Might actually be Getting Smilies Painted on Your Soul, since none of the denizens seem to dislike living in Cancer's realm.
Yes-Man: While we don't see her interact with Capricorn, it's suggested she is this towards her, as all of her profits go to Capricorn and the reason she invited Virgo to the Fortress of Dolls in the first place was so that she could avenge the murder of Capricorn's daughter Deneb Algedi. Virgo even calls it bootlicking in the "The Reason You Suck" Speech she gives to Cancer.
Know When to Fold 'Em: Libra gives up her crown without a fight. She was never the strongest among the Zodiacs, so she knew she would die if she tried to fight Virgo, considering how strong she has become by the time the two meet in person.