Adventures of the Galaxy Rangers: Piss off Captain Foxx and you'll get one of these. Even Gooseman backs off when the stare comes out. You will not get a second warning before the Arm Cannon warms up.
Vi has a pretty humorous example in Arcane. After a job gone wrong, Mylo is verbally raking Powder over the coals for fleeing the scene and losing their payload in the river. Vi retorts that Mylo is just as bad, saying that he never knows when to shut up.
Vi: I'm gonna help you with that. [Pointing to her face] This will always mean "shut up!"
Mylo: But-
Vi: [Gets even angrier]
Mylo: I-
Vi:[Her expression goes beyond death glare territory and into sheer disbelief.]
The Adventures of Paddington: Paddington often gives a "hard stare" to everyone whom he thought is already too much or gone too far. Notably, to Mr Curry and Mr Brown.
Talia al Ghul gives one to Terry for misspelling Ra's al Ghul's name, yet strangely does not give one for him calling Ra's al Ghul a psychopath, instead settling for a horrified look of shock. That's because it's Ra's al Ghul in Talia's body and would have every reason to be horrified by the notion of him being a psychopath as well as every reason to be annoyed at having his name mispronounced.
Poison Ivy maybe a sensuoussiren but rile her up and you'll find she's quite the vicious bitch. Batman discovers this firsthand in "Pretty Poison" when he kicks her to the ground and cuts himself free from her mutant flytrap's tendril grip. After that Ivy switches from flirting to fighting, shooting her crossbow arrows at him as she screams "murderer!" all while maintaining a ferocious, venomous stare.
The Beatles: At the end of "Penny Lane," John, George and Ringo give Paul a quick death glare before chasing him down the street after they find that the crime in Penny Lane that Paul thought was going to occur turned out to be a pending crime on heiress Penelope Lane, which secret agent James Blond had already solved.
in one episode of "Ben and Holly's Little Kingdom", there's a Running Gag where Holly gives a death glare to Strawberry whenever she says this because it was Strawberry's fault that Holly's wand broke.
Strawberry: She bashed it on a rock.
Holly"': gives a Death Glare''
Bluey: Chilli will shoot an intense glare at her husband or daughters to let them know they're taking a game too far and she's being completely serious.
A case of a near-literal Death Glare in Captain Planet and the Planeteers, during a trip back to World War II, a very badly rendered Hitler — sporting a bowl cut and handlebar mustache — once nearly took the titular superhero down with a cold, dispassionate stare fueled by the "pollution" of hate and bigotry in his heart. By contrast, remember that Superman looked Hitler in the eye and kicked his ass at least a dozen separate times in WWII-era comics. Hatred is considered Heart-flavored pollution, so Hitler is a walking block of kryptonite to Captain Planet.
Mr. Sunshine from CatDog gives everyone he meets this because of being The Cynic.
This was essentially Stewie's default facial expression in earlier seasons of Family Guy.
In "Franklin and Beaver's Show-and-Tell" from Franklin and Friends, Beaver has a magnificent one for Franklin after he asks why she didn't tell him a bee was chasing her, even though she had been trying unsuccessfully to communicate this through charades because she lost her voice.
Franklin: Oh, a bee was chasing you?! ... Why didn't you say so?
Beaver: gives Death Glare
Franklin: That was just a joke.
Gargoyles: Goliath is, by all appearances, a master at this (perhaps partly due to his thickly-muscled brows); but when he's really angry — complete with Glowing Eyes of Doom — well, you'd best start backing away PDQ.
Demona's no slouch in the glare department, either.
Max and P.J. each give a nasty one in the Goof Troop episode "Waste Makes Haste", when Pete reveals his real objective during their recycling gig with Goofy: To scavenge for a pair of missing golden baby shoes that Pete intended to return to the owner... in exchange for a hefty reward he would keep for himself. (P.J.'s stare actually borders on something downright Kubrickian.) Goofy, however, appears unfazed by this revelation.
Mr. Cat has managed to give out some pretty terrifying ones to people who bother him, causing the other person to quickly back off.
Kaeloo gives one to Stumpy in the tea party episode when he won't stop annoying her. The glare is so scary that Stumpy immediately covers his mouth with both hands and stops talking.
Hank Hill of King of the Hill is a master of this. His trademark glare is the one warning belligerents get before his boot meets their ass. 9 times out of 10, they wisely back off.
Peggy also tends to give Hank glares of disappointment to make him help his family with their problems.
In The Legend of Korra, Pema gives Tenzin one for reluctantly allowing Tarrlok to join them for dinner, especially after Tarrlok says that Air Nomads never turned away a hungry guest. Moments later, Ikki trumps her mother by giving Tarrlok a longer one, which isn't noticed by those at the table but noticed by the audience. Bonus points added for Ikki's glare looking like Pema's glare.
Bolin gets one from almost everyone for being Innocently Insensitive about Korra losing her bending.
Bolin also gets one from Suyin for pushing her out of the way to hug her daughter whom they’d all just rescued.
P'Li gives a rather scary one right at the start of "In Harm's Way".
Fire Lord Izumi gives a very motherly one (fitting as she is one) to Prince Wu in "Beyond the Wilds" when he won't stop interrupting their world leaders meeting,
Another example as a Lame Pun Reaction is in The Magic School Bus "Makes a Stink", when Carlos's pun about nasal mucus earns him one from Ms. Frizzle, of all characters.
On Mission Hill, death glares are often accompanied by daggers coming out of the person's eyes.
Moonbeam City: Pizzaz and the Miller sisters have a shared one. Whenever they use that particularly glare, an ominous music starts playing and Venitian blinds shadows appear over their face, even when there is no window around to cast that shadow.
It's actually a plot element in "Stare Master". Fluttershy is somewhat renowned for this ability of hers, but she cannot control it. It may sound funny, but the Stare seems to possess nigh-hypnotic properties, able to break the will of any creature meeting it. This ranges from rounding up some disobedient chickens to staring down a cockatrice, a creature with the power of turning living beings to stone with a glare, until it folds and gives life back to its victims. That's right, Fluttershy's Death Glare reduced into submission a monster with an actual Magical Eye.
This limit and responsibility are enforced in "Bats!", when her friends convince her to use her stare to drive an infestation of Fruit Bats away from Applejack's farm. Twilight helped to enhance Fluttershy's ability, but it goes From Bad to Worse.
In "Keep Calm and Flutter On", Discord is shown to be immune to Fluttershy's Stare.
In "Party of One", Pinkie Pie also displays some scary glares during her episode-long Sanity Slippage.
In "Swarm of the Century", the ponies have conveniently dealt with the problem at hand a solid ten minutes earlier than usual, only to learn Fluttershy couldn't help but keep one of the rapidly multiplying Parasprites for herself. Cue epic death glares from Twilight Sparkle, Applejack, and Rarity.
In "Family Appreciation Day", during a flashback to Granny Smith's youth, her mother angrily glared at her father after he kissed Princess Celestia's hoof in gratitude right in front of her.
In "The Mysterious Mare Do Well", Rainbow Dash rescues a baby, but she gets so caught up in the fame and congratulations she receives that she carelessly tosses the baby over her shoulder without noticing. The mother catches the baby and glares at her.
In "Three's a Crowd", Twilight gives one to Discord upon learning he only faked his Blue Flu to test her if she's trustworthy enough to accept her as a friend. Discord's response even sums it up.
Discord: Why the angry eyes?
In "Rarity Investigates!", Rainbow Dash gets a full round of them from the Wonderbolts when she is suspected of sending Spitfire a fake letter that her mother was sick which led to her disappearance.
In "No Second Prances", Applejack gives Starlight Glimmer three death glares in a row after Starlight uses her magic to turn Big Macintosh into a Motor Mouth.
In "The Times They Are a Changeling", everyone in the Crystal Castle gives a round of death glares to Spike as he is singing his song about Thorax and how he has reformed.
The best one would probably be in the Season 6 finale, "To Where and Back Again", as after Queen Chrysalis erupts from the ruins of her destroyed Anti-Magic throne, ready to fight with her horn flaring and a murderous hiss in her throat, her anger quickly changes to fear when she sees what awaits her: All of her cocooned victims, including the Royal Family, the Elements of Harmony, the Master of Chaos, and Trixie, are free of their prisons, their magic fully accessible again, and joined by her reformed Swarm and their new King, Thorax, they all are giving her various looks of the utmost anger and fury over what she's put them through in the last few hours, if not days, just for her Revenge for the Royal Wedding defeat. Even Baby Flurry Heart looks outright angry at Chrysalis for what she's done. Chrysalis herself then gives one to Starlight when she turns down her chance at redemption and vows revenge on her for destroying her throne and ruining everything.
Rick and Morty: In "Meeseeks and Destroy" Rick Sanchez quickly and quietly realizes that Morty was nearly raped by King Jellybean. He doesn't say anything to upset or insult Morty, only giving a look that mixes horror and Tranquil Fury. In fact when Rick and Morty return to earth, Rick quickly grabs a gun and kills Jellybean through the portal. Rick didn't want to ruin Morty's adventure and made sure that it goes great for him without Rick ruining it but as soon as Morty's back is turned, Rick doesn't hesitate to murder his most deserving victim.
In episode "Lady Bouvier's Lover" (#1F21), Abe Simpson had his dance-party date (Marge's mom) usurped by a rival. Abe angrily declared "I'm gonna give him the frowning of a lifetime!" and proceeded to do so. Unfortunately, the rival was the pathologically ego-centric Mr. Burns, so Abe's efforts were completely ignored.
Mr. Burns is no slouch in the Glaring department either. (He calls it his "glower power.")
In the episode "Saddlesore Galactica", during the victory montage of Furious D. (A horse that Bart adopted, and turned into a racing horse), one section has the titular horse run first out of it's stable only to give a death glare towards the other horses, complete with a dramatic zoom. The other horses proceed to reverse into their start boxes and close themselves in.
Marge does this very frequently, almost always accompanied by her disapproving moan.
One of the Rewatch Bonuses in part one of the famous "Who Shot Mr. Burns" two-parter is everyone averting their eyes when Mr. Burns challenges them to hurt him — expect Maggie, who delivering one of these at him.
Sofia shoots a very hard one at Amber in the Pilot Movie when she finds out she tricked her and ruined her dance lesson. James also gives her one seconds later.
In "Day of the Sorcerers", Sofia gives one to Cedric as she faces off against him in the throne room and he struggles with the decision to freeze her with his Medusa Stone.
The Spectacular Spider-Man: Gwen Stacy doesn't quite have one of these, but she does have "the look" which has roughly the same effect on her (male) friends Peter and Harry. It also apparently works on her dad (though he may have been joking), which is pretty impressive considering he's voiced by Clancy Brown.
Star Trek: Lower Decks: In "Terminal Provocations", Badgey's eyes express his homicidal rage after the holodeck safety protocols are deactivated.
In the Star vs. the Forces of Evil episode "Monster Arm", the guy that sat next to Star on her right gave her one. Note he still gave her one when she's not sleeping on him.
"R2 Come Home": When it's revealed that the bounty hunters have taken hostages, Admiral Kilian, Commander Ponds and the clone navigation officer, being Bound and Gagged, spend their time shooting glares at their captors.
"Carnage of Krell": Captain Rex and General Krell at one point briefly get into what can only be described as a "glare-off"*Complete with Krell, a hulking Besalisk, bending over to get right into Rex's face, and if they hadn't been interrupted by a conveniently-timed transmission things might have gotten very interesting in that control room.
"The Disappeared, Part 1": While fighting the Frangawl cultists, Mace Windu gives one cultist a glare so terrifying that the cultist opts to run away rather then even try and fight the Jedi Master.
Ezra Bridger pulls out a very lethal glare when he gets angry enough. A prime example is in "Gathering Forces", when he has a look of pure death on his face as he taps into The Dark Side to summon a giant fyrnock against the Inquisitor.
Ezra's own ignorance has had him on the receiving end of these, such as the time he asked why "Mandalorians couldn't just make their armor out of something else," which earned him at least a half dozen epic ones from every Mando in the room, including his friend Sabine.
In the Wham Episode"Mirror Gem", Garnet gives one of the most terrifying death glares to Steven after he accidentally hit her face when she tried to take Lapis Lazuli's mirror.
Super Robot Monkey Team Hyperforce Go!: You do NOT want Nova glaring at you. EVER. Likewise, while this occurs less often, Antauri has quite the death glare when it chooses to come out.
In an episode of the 2012 Teenage Mutant Ninja Turtles Leo, Donnie, and Raph do this to Mikey on several occasions.
Teen Titans: Raven has a Death Glare that will make you shit bricks. Ask Doctor Light. Beast Boy is apparently immune to it (probably through repeated exposure), as when she gave him one in the episode "Fear Itself", he was unfazed. Occasionally he does back off, it's just that it's not a death glare to him.
The episode "Betrayal" contains a Played for Drama example - just after getting a Heel–Face Door-Slam from Beast Boy, the last expression seen on Terra's face as she and Slade slip into the shadows together is an absolutely chilling glare in BB's direction.
In the CGI era of Thomas & Friends, The Fat Controller has given these to the engines on occasions when they get out of line. One example includes in "Duck and the Slip Coaches" where he gave one to James after finding out that he stole Duck's idea on the Slip Coaches. Cue James' "Oh, Crap!" Smile.
In the Thunder Cats 2011 episode "Berbils" gruff Old Soldier Panthro delivers one when he's caught openly hugging his new friend Ro-Bear Bill:
Wilykat: Busted! Wilykit: Panthro's a big ol' softy! Panthro: Is that a problem? Wilykit:[stiffens nervously] Nope. Wilykat: It's cool.
In the original Thundercats 1985, during the second part of The Feliner, Slithe, Monkian, and Jackalman smart off at and outright challenge the authority of their former leader, Ratar-O. He gives his former henchmen a very nasty one... before whipping out his weapons, the Rat's Eye! Vultureman wanted no part of what the other three got. They got
Courtney has a fearsome death glare which she regularly gives to both her enemies and her beloved bad boy boyfriend, Duncan.
Owen earns two of these from Noah in "I See London", the first is even accompanied with a Scare Chord.
Gwen had many of these over the course of the series.
Dawn from Revenge of the Island gives a surprisingly effective one prior to her elimination after Scott sabotaged their team and framed her for stealing everyone's stuff.
Despite not having a face, Soundwave manages to pull this off. Right before giving Airachnid an epic beatdown.
In "One Shall Fall", Optimus Prime,of all bots, pulls off a shocking Death Glare worthy of the Dark Knight himself. It's both awesome and terrifying all at once. You know the shit just got real folks when a bot like Optimus acts out of character like that. What caused it? Megatron on Dark Energon blasting Bumblebee while he took Raf home from the Autobots' base. What resulted is that Raf nearly died from toxic exposure to Dark Energon and Megatron was stupid enough to flash Prime a Slasher Smile while gloating over the matter. His Roaring Rampage of Revenge against Megatron for nearly killing Raf is full to the brim with hulk rage. His voice is controlled, absolutely calm and his pace methodical as he cuts down any Decepticon between him and Megatron. Although to see him express the intent to murder Megatron and (if Megatron wasn't powered up by Dark Energon beforehand) actually succeed in following through should make it quite clear Optimus was in a state of mind and fit to kill Megatron on sight.
The Transformers: Back in Generation 1, the Decepticons successfully framed the Autobots and made themselves look like the good guys. While Soundwave and his cassettes attended a teen dance, some guy asked "Laserbeak wanna cracker?" No. Laserbeak did not want a cracker.
Whirl: Word of advice, when my eye does this, clear the area.
Wakfu: Ruel's grandmother will give you a Death Glare if you ever say the word "borrow". Or "discount". Or "refund". Or... well, any suggestion you could maybe pay less than her tariff. Even... especially to her grandson — he should know better.
On Wonder Pets!, Linny gives Ming-Ming one when, in "Save the Visitor!", Ming-Ming suggests of the Visitor, "Maybe he's a guinea pig. He sure eats like one." (Linny is a guinea pig.)
