Usually Spiders Are Scary, especially if they're giant or so many. But sometimes, spiders can be actually nice and harmless. Sometimes can be used as a pet or an ally, or sometimes, if is a spider person, can be part of the hero team.
Positive portrayals of spiders in fiction tend to emphasize different aspects of them than negative ones do. Often, the fact that they eat insect pests will be mentioned, and their web-building habits are often used as an example of perseverance and industriousness. Negative or evil portrayals of spiders often play up their use of venom (because Poison Is Evil, after all). Heroic spiders, therefore, are rarely described as being venomous.
It is also worth noting that not all spiders are seen in an equally favorable light. Tarantulas and black widow spiders, which are almost universally considered dangerous (though this is quite exaggerated in fiction compared to reality, especially in the case of the former), rarely get this treatment. Jumping spiders and orb-weaver spiders, on the other hand, are more likely to be portrayed positively since they are considered harmless and look conventionally "cute" to many people.
Contrast Spiders Are Scary, the inversion of this trope. Some Spider People can be seen as this. A Friend to All Living Things usually has one as friend. Sub-Trope of Good Animals, Evil Animals, in which the spiders are usually Funny and/or Talking Animals, as well Dark Is Not Evil, in which spiders are usually being of color black or another dark colour.
- The arachne Rachnera Arachnera from Daily Life with Monster Girl is one of the many monster girls in the Unwanted Harem of Kimihito. She hated humans before until she met Kimihito and eventually fell in love with him and made friends with the other monster girls. Rachnera is aware of her own strength, so she's very careful with Kimihiko, also saving him in a couple of times. She is, however, aware that humans think Spiders Are Scary and is very, very willing to take advantage of that fact.
- Invoked with Spider-Man, a superhero with a spider-theme, who also has the Red Baron of being "Your Friendly Neighborhood Spider-Man", usually being nice to people and being the savior of New York countless times. Also, this is extended to various of his spider-allies as well as his Alternate Continuities alter-ego (as well as his allies like Spider-Gwen).
- Also invoked with Black Widow, who also has a spider inspiration as Spider-Man but with a different scenario and origins (a Badass Normal Femme Fatale Spy instead of a superpowered being), being part of The Avengers as well S.H.I.E.L.D., even when she's not so nice as Spidey.
- In Madagascar, Alex finds a big hairy spider on his shoulders. The only thing the spider does is give him a big friendly smile and a cheery "Well, howdy-do", but Alex freaks out anyway.
- In A Bug's Life, Rosie, the black widow spider (and a relatively rare instance of that species filling this trope), is the gentle Team Mom of the circus bugs. She provides the current trope image.
- In Corpse Bride, there are some friendly, talking black widow spiders in the world of the dead. One of them is a close friend of Emily, the titular corpse bride.
- In Mune: Guardian of the Moon, there is a race of cute, fluffy spider-like creatures that assist the current Guardian of the Moon. Although they occasionally display Silent Snarker personalities, they are still shown to be benevolent and helpful.
- Buzz McCallister, the Big Brother Bully of Kevin in Home Alone, has a tarantula (Grammostola rosea) as a pet, which was released after Kevin went home alone. This was eventually used as one of Kevin's Improvised Weapons against the Wet Bandits. In fact it could be argued that the spider, like the cellar was part of Kevin's childhood fears which he had to overcome in order to rely on himself more and protect his home. And what better overcoming is there other than using the once source of mortal terror into a weapon of defense against the real enemies? In the end, the tarantula does not receive damage and it was found by Buzz when he came back home with the rest of his family (or at least is implied).
- Charlotte's Web (as well their adaptations) is about a friendly spider that helps prevent the pig Wilbur from being slaughtered, even giving her own life to save Wilbur's as well being a kind of godmother for the rest of the farm.
- Being Friend to All Living Things, Rubeus Hagrid from Harry Potter has a Giant Spider called Aragog, which was adopted by and is loyal to Hagrid, being defended by him from the accusations of Aragog being Slytherin's Monster, which was the basilisk instead. Unfortunately, subverted when Harry and Ron discover Aragog isn't nice at all, especially because his species has a taste for human flesh and he doesn't extend the exception that he makes for Hagrid to Hagrid's friends.
- The Four Horsemen Universe: Double Subverted with Tortantulas, a race of car-sized arachnids that are first introduced as Blood Knights in Cartwright's Cavaliers, known for loving to fight and eating their kills. In the next two books, though, there are Tortantula mercs in the supporting cast that often act as Comically Serious comic relief: as a race they are fairly Literal-Minded and so social interactions between them and mammals often take strange turns.
- In James and the Giant Peach as well as its animated film adaption, there is a friendly spider appropriately named "Miss Spider". In the book, she is more of a grandmother-like figure, while in the film she is darker and more gothic, but still all around good.
- Arachne from Alex Verus, who is also of the Giant Spider variety. Close friend of Alex's who likes to make clothing. Her tendency towards No Sense of Personal Space (she's extremely nearsighted) tends to unnerve people who don't know her well.
- The Addams Family: The title family have a pet spider named Homer. Despite the neighbours being scared of him, he's actually quite a nice pet.
- In African mythology, Anansi the Spider is a Trickster who is generally the protagonist in his stories, and is usually regarded as benign or beneficial.
- A more benign interpretation of Arachne from Classical Mythology is that Athena turned her into a spider that's generally harmless and would only weave webs. Of course, considering that it's the Greek myth and their Gods, so it's often a common practice to think that Arachne got bitter at this and turns the other way around.
- In the The Wubbulous World of Dr. Seuss episode, "A Bird's Guide to Health", Horton the Elephant meets Sid the Spider, a spider whose left legs were crushed by a fallen tree. Despite Horton's initial fear of spiders, he and Sid become friends. Horton nurses Sid back to health and protects him from Jane Kangaroo and the Wickershams, who all think that bugs are creepy and not friendly.
- Skid from TinkerQuarry is a living plastic toy spider who exhibits a Cheerful Child-like personality, and is one of the sweetest and kindest characters in the game.
- Kirby Star Allies: While the spider-like Como enemies have appeared in previous games, they were almost always portrayed as generic spider enemies. In this game, however, they can be transformed into Helpers.
- In Donkey Kong Country 2: Diddy's Kong Quest and Donkey Kong Country 3: Dixie Kong's Double Trouble!, Squitter is a spider who assists the Kongs in their quests. He creates two different types of webs; ones that can be used as ammunition against enemies, and ones that can be used as temporary platforms to get across dangerous places or reach higher places and/or items.
- Moshi Monsters has a minor character who's a spider named Scratch, who once helped the Super Moshis save Simon Growl from a fall by using a web.
- In Pokémon, Spinarak from Second Generation and Joltik from Fifth Generation are Pokémon depictions of spiders that actually are cute and nice, also seen in their appearances in the anime. However, their evolutions (Ariados and Galvantula, respectively) may look scary, but in the end all depends of their trainers.
- Questionable Content has Gordon the AnthroPC, a congenial AI who doesn't quite understand that his cartoonish mini-Spider Tank chassis is pure Nightmare Fuel to a lot of his clients.
- Lucas the Spider is a YouTube video series dedicated to making the titular young spider as tooth-erodingly cute as possible.
- French biologist and cartoonist P-L Meunier made this comic about this "sleep assistant".
- In Pocoyo there're a few secondary spider characters with one eye who are despised even though they're actually nice. A good example is the episode "Pato's Egg" in which Pato finds an egg and raises it, only to find that it hatches a spider instead of a bird. Everyone finds it scary, including Pato, but later it is seen that this baby spider is very nice and is embraced by Pato as his father, and later is accepted by his friends Pocoyo and Elly. Also is seen later in other Pocoyo shorts, like "The Big Race!" having his own car.
- Jeff from The Grim Adventures of Billy & Mandy, a Giant Spider who is friendly and kind and thinks Billy is his father. Billy, on the other hand, is dreadfully afraid of spiders, and always tries to swat him whenever he appears.
- The two main characters of the Hungarian animated series Vízipók-Csodapók are a water spider and an orb-weaving diamondback spider. The water spider is a wide-eyed, curious character who is friendly with all bugs he meets, whereas the diamondback is somewhat grouchy and snarky, but still a loyal friend.
- My Little Pony: Friendship is Magic: In "Maud Pie", the resident Friend to All Living Things Fluttershy introduces Maud Pie to a spider, describing this particular species as "actually very sweet and help keep other, more dangerous insects away!" And then the spider offers a flower to Maud. This would also count as a subversion of Spiders Are Scary, since the spider is introduced glaring menacingly from solid red eyes and with fangs dripping with saliva, presenting a traditionally scary image before offering its flower. The spider goes on to reappear in later episodes as part of Fluttershy's menagerie of friendly woodland critters. "Scare Master" reveals that his name is Fuzzy Legs.
- Charlie and Lola: In the episode "I'm Not All That Keen on Spiders", Lola meets two spiders named Sidney and Robert, who she's scared of at first, but then her Cool Big Bro Charlie tells her spiders are nice.
- In the Rugrats episode, "Spike Runs Away", after Spike runs away, Stu and Didi try to get Tommy a replacement pet. One of these is a tarantula named Terry, who isn't poisonous, and has even been defanged so he can't bite. However, Didi still finds Terry creepy. When Tommy opens the door to Terry's cage to try to teach him how to fetch his ball, Terry crawls into Lou's bedroom, where he frightens a sleeping Lou. Lou, having not been informed of Terry's presence, kills him, wraps him in a wad of tissues, and tosses him into a trash can.
- Miss Spider's Sunny Patch Friends stars the kindly Miss Spider, who adopts three insect children along with raising her own five spider kids. There is another spider character, Spiderus, who starts out as a bad guy, but becomes more of a Jerk with a Heart of Gold as the series goes on, as well as Miss Spider's husband Holley and Spiderus's sweet wife Spindella.
- The Looney Tunes Show: In the season 2 episode "Itsy Bitsy Gopher", Bugs and friends are afraid of a big spider that appears in their house. After failing attempts to kill the spider, later appears Gossamel looking for Webster, his pet spider (whose species is a mimicry of the spider characters are chasing for) which is harmless.
- Grammostola rosea, also called as "Chilean rose tarantula"note , is a tarantula species originated on Chile, which is basically a hand-size haired tarantula which is nice, friendly and harmless. This is probably the only spider that is considered as a pet and is usually treated as that in this country as well in various parts of the world, usually as "exotic pet". Truth in Television the spider used in various movies and series as pets is the Grammostola rosea, as in the Home Alone example.
- One of the benefits you'll often hear about having spiders around is that they'll get rid of all the other bugs you don't want around. This makes them friendly (or at least helpful) for humans, but not so much for insects...
- Spiders in general are not exactly friendly, but at the same time most won't attack you unless you give them a reason to. Most spiders, even infamously dangerous ones like the black widow, much prefer to skitter away from humans than bite them on sight. (Evolutionary logic: poison is costly, don't waste it on non-prey if possible.)
- Jumping Spiders are about the size of a dime and have a quartet of Puppy-Dog Eyes framed by four tiny eyes.