Played by: Timothy Moxon (on-set actor), Robert Rietty (voice)
He is the first British spy to appear on screen in the franchise. He lives in Jamaica, has a membership in the Queen's Club and investigates Dr. No's activities around Crab Key island. He is shot down (along with his secretary) by the Three Blind Mice at the beginning of the movie and the novel, and all of his files about Dr. No are stolen. His disappearance prompts the MI-6 to send James Bond to Jamaica.
- Card Games: He played cards with fellow members of the Queen's Club just before his assassination.
- Eyepatch of Power: He's got one eye in the book.
- He Knows Too Much: He and his secretary are killed by the Three Blind Mice due to the informations they gathered about Dr. No's activities on the isle of Crab Key.
- Hero of Another Story: Strangways was investigating Dr. No's activities in Crab Key island prior to his assassination.
- Plot-Triggering Death: His murder kicks the plot (not to mention the series) into motion.
- We Hardly Knew Ye: In the film, he's killed after one minute of screen time, give or take. He holds the dubious distinction of being the first person to die on screen in the James Bond film franchise. Ironically, in the book, he was a supporting character in Live and Let Die, so he's Back for the Dead in this one instead.
Played by: Dolores Keator
The secretary to John Strangways, operating the British Secret Service station in Jamaica. Unaware that Strangways has been murdered, she proceeds to make contact with London for their scheduled transmission. Hearing a noise, she proceeds to investigate, only to find Strangways' killers, the Three Blind Mice, who kill her. Afterwards, they carefully remove all the Service's files relating to Crab Key and Dr. No.
- All There in the Manual: Named Mary Trueblood in the novel, she goes unnamed in the film, and the only reference to her name is that she's credited in the film as "Mary" (only "Mary").
- He Knows Too Much: She and Strangways are killed by the Three Blind Mice due to their information about Dr. No's activities in Crab Key island.
- We Hardly Knew Ye: She probably has less screen time that even Strangways. She also holds the dubious distinction of being the first woman to die on screen in the franchise.
Honeychile "Honey" Ryder
Played by: Ursula Andress, Nikki Van der Zyl (voice), Diana Coupland (singing voice)
The first main Bond Girl in the film series. Her iconic first appearance has her coming out of the ocean for beachcombing in Crab Key. She then accompanies Bond and Quarrel during their exploration on the island and Dr. No's facility.
- Adaptational Attractiveness: In the book she's also pretty, but her nose is broken (she even sells her shells to pay for the operation).
- Adaptational Modesty: In the book, she's completely naked when Bond meets her. This would never have met with the censor's approval in 1962, so she wears a white bikini when coming out of the water in the film.
- Always Save the Girl: As Crab Key is about to explode, Bond makes sure to ask where Honey is kept to bring her along.
- Bare Your Midriff: From her introduction until being brough to Dr. No's lair, she's in a bikini.
- Beauty Is Never Tarnished: Even hiding in swamps or kept captive she remains tidy.
- Distressed Damsel: By the movie's end. And since Bond goes by Always Save the Girl, why would a Collapsing Lair stop him?
- Girl of the Week: The main Bond Girl for this film, and the first of many Bond Girls with whom Bond ends up with by the end of a film.
- High-Class Call Girl: In the book, she tells Bond she plans to become one of these, so she can get the money for her operation. Bond, however, points out why it's a bad idea.
- Innocent Fanservice Girl: Played with in the book. She casually mentions her ambition to work in New York as an escort girl, and is unaware that society would find this idea objectionable.
- The Load: In the film, she just goes along with Bond instead of helping him do anything. Averted in the book, where she warns Bond of incoming threats as they drive the Dragon to safety, is a skilled sailor, worked out how to sell her seashells for profit very quickly and escapes on her own without Bond's help.
- Ms. Fanservice: Her intro is even wearing a bikini.
- Naked First Impression: How Bond meets her in the book.
- Rape as Backstory: And she replied by putting a female black widow spider in the guy's bed.
- Same Language Dub: Given the actress had a thick accent, she was dubbed over. It's a Non-Singing Voice as well, as the composer's wife sung for her.
- Troll: Has shades of this in the book, interestingly enough. She hits on Bond repeatedly in Dr. No's lair, after Bond has established that he's reluctant to seduce her.
Played by: John Kitzmiller
A Cayman Islander who was employed by John Strangways to secretly go to Crab Key to collect rock samples. He also worked with Felix Leiter before Bond's arrival, and goes to explore Crab Key with Bond.
- Black Best Friend: He quickly becomes Bond's friend and is liked by everyone in his district.
- Black Dude Dies First: The first and only ally of Bond to die in the movie.
- But Not Too Black: In the book, Fleming takes great pains to point out that you can tell he has at least one white ancestor. This isn't mentioned in the movie.
- Kidnapped by an Ally: Does this to Bond the first time they meet.
- Kill It with Fire: Dr. No's men roast him with the "Dragon tank".
- Only One Name: He only goes by one name.
- Sacrificial Lion: The first of the many allies of James Bond to die in the series.
- Would Hit a Girl: Suggests to break the photographer's arm.
Doctor Julius No
Played by: Joseph Wiseman
The first villain in the film series. He is a half-German/half-Chinese scientific genius working for the villainous SPECTRE organization (SMERSH in the novel). He is using his private nuclear laboratory to knock American rockets out of the sky in the film and sabotage American missile launches in the novel.
- Antagonist Title: His name gives the film (and the novel) its title.
- Artificial Limbs: Having lost his real hands to radiation damage, he's replaced them with metal prosthetics, which are very strong, but lack dexterity. Their lack of grip costs him his life.
- In the novel, the Tongs chopped his hands off after he stole them a million dollars worth of gold when he was their treasurer. In the film he still robbed them but managed to get away with it, and he implies that his hands were an accident during his research.
- Bald of Evil: His physical appearance in the book.
- Big Bad: The main villain of the film, with his backing organization being behind the scenes.
- Bond Villain Stupidity: Mostly averted. Bond is completely in Doctor No's power for most of the movie. Still, he's the first example in the film series of "Why Don't You Just Shoot Him?"
- Diabolical Mastermind: Plans to ruin the American spacecraft/missile launch, no matter what.
- Dirty Communist: In the novel only, where he expresses interest in working for Communist China. In the movie he expresses his disgust with both the East and the West, dismissing them as both equally as stupid as the other; although, given the number of Chinese running around his base and the mercenary nature of SPECTRE, it's possible that Communist China are the ultimate backers of his evil scheme.
- The Dreaded: One assassin he sends commits suicide rather than give any information to Bond, a photographer would rather have her arm broken than admit who she is working for, and Dent is clearly terrified of him. Basically, everyone who works for No is scared to death of crossing or failing him.
- Evil Cripple: His hands can break solid rock, but they aren't very dexterous, resulting in his death.
- Evil Genius: With a truly ambitious scheme. In the film, it's to disrupt the Project Mercury launch with an atomic-powered radio beam. In the book, it's to not only disrupt missile launches so he can sell the remains to Soviet Russia, but also to start a jamming war until he can hijack the missiles well enough that they drop on Miami and Kingston.
- Expy: He was modelled on Fu Manchu.
- Freudian Excuse: It's implied the reaction to his mixed heritage led to his FaceHeel Turn.
- Handicapped Badass: Besides being strong enough to crush a metal figurine, his mechanical hands also give him one hell of a punch, as seen in his short fight with Bond at the end of the film. Too bad for him their lack of manual dexterity puts him at a disadvantage while grappling and makes it impossible for him to climb out of a nuclear reactor.
- Heart in the Wrong Place: In the book, he tells Bond how he survived reprisal from the Tong after embezzling funds - after hours of prison torture, they chopped off his hands and shot him through the heart, or thought they did. As it turned out, he was a rare case with his heart on the right side of his body.
- Hoist by His Own Petard: His metal hands do not have enough grip to allow him to climb out of the superheated pool of water.
- Iconic Outfit: His white Nehru suit.
- Karmic Death: Falls into a bunch of boiling radioactive water in the movie and gets crushed by a crane load of guano in the book.
- Lean and Mean: He's tall, thin and the main villain of the film.
- Mad Scientist: He's a genius in nuclear physics working for an evil organization, who uses his knowledge to ruin the American spacecraft program and when he's not wearing Nehru suits he wears lab coats. Yes, he qualifies.
- Man in White: When it's not gray.
- Man of Wealth and Taste: Wears a Nehru suit, and his lair has respectable decoration and bar.
- Morally Ambiguous Doctorate: He's very evil and, as seen above, a mad scientist to boot.
- Not That Kind of Doctor: He's a scientist, not a medical doctor.
- Red Right Hand: His mechanical hands.
- Soft-Spoken Sadist: His voice is calm and polite when talking to Bond.
- Starter Villain: Of the film series. His defeat kicks off Bond's long standing rivalry with SPECTRE.
- The Stoic: To the point where the only time he loses his cool is when he falls into the boiling radioactive water.
- Supervillain Lair: In the book, it is highlighted this is very irregular. Becomes a Collapsing Lair.
- We Can Rule Together: Doctor No offers Bond the chance to join SPECTRE... or intended to until Bond spent their dinner mocking him.
- Why Don't You Just Shoot Him?: An Unbuilt Trope and the first classic example. Doctor No could have saved himself a lot of trouble this way, but he was Justified since he wanted to recruit Bond into SPECTRE and, finding out this was not possible, seemingly planned to torture him for information before killing him but had to deal with toppling the latest American missile first.
- Wicked Cultured: Many, many James Bond villains have taste and class, but Dr. No spent one million dollars on an underground fish tank, and Bond is amazed to see Goya's Portrait of the Duke of Wellington.note He's no slouch either in the book, with a vast knowledge of philosophy and stamp collecting.
- Yellow Peril: A low-key example given he's a half-Chinese man working for a white-run organization. His introduction theme does have a Asian-esque sound to it, however, and the majority of his base staff seem to be Asian (though his outside operatives are from a number of different races). The character was intended to be a tribute to Fu Manchu. Indeed, one of the actors considered to play him was Christopher Lee, who portrayed Fu Manchu in the most number of films of any actor.
Professor R. J. Dent
Played by: Anthony Dawson
A geologist with a practice in Kingston, he is also a member of the Queen's Club. He secretly works for Dr. No, has Strangways killed and tries to kill Bond on two occasions.
- Animal Assassin: Dr. No orders him to put a tarantula in Bond's bed.
- Bad Liar: Bond sees through his lies about Strangways' mineral samples from Crab Key pretty quickly.
- The Dragon: He is Dr. No's main agent in mainland Jamaica, and the most prominent named henchman Bond has to deal with before going to Crab Key.
- Genre Blindness: Bond has a particularly memorable Pre-Mortem One-Liner about this:Bond: That's a Smith & Wesson, and you've had your six.
- The Heavy: The most prominent threat before Bond meets No.
- The Mole: He is Dr. No's mole at the Queen's Club.
- There Is No Kill Like Overkill: He empties his entire magazine into the dummy in bed that he thinks is Bond. This quickly bites him in the ass, as he has no bullets left to defend himself when the real Bond gets the drop on him.
- Smug Snake: Denies anything when Bond tries to extract info from him. And then goes for murder attempts, the second of which gets him caught in a trap by Bond, who shoots him.
Played by: Zena Marshall
Dr. No's spy posing as a secretary at the Government House in Jamaica. She tries to lure Bond into a trap in her house.
- Bad Liar: She comes with flimsy excuses when trying to keep Bond at her home until assassins arrive to kill him.
- Honey Trap: When the above bad lies fail she resorts to this, with... better results. For a while, at least.
- The Mole: A secretary at the Government House in Jamaica who also is one of Dr. No's spies.
- Ms. Fanservice: Is even wearing only a towel at a certain point.
- Sexy Secretary: Making it even easier for Bond to consider getting info outta her.
- Spiteful Spit: To Bond when he has her arrested.
- The Vamp: Seduces Bond and the two sleep together in order to keep him at her home until assassins arrive to kill him. It doesn't work.
- You Have Outlived Your Usefulness: Downplayed. Taro survives and is merely arrested; however, when Dent shows up to her house to kill Bond, he just shoots whoever is lying in the bed, meaning that he was likely trying to kill both of them (in the case of Taro, likely just trying to tie up loose ends).
The Three Blind Mice
Played by: Eric Coverly, Charles Edghill and Henry Lopez
Three Jamaican hitmen working for Dr. No. They move around in a hearse and pretend to be blind beggars as a ruse to deflect attention when approaching their target. They were the very first characters to appear on screen in the Bond films, right after the opening.
- All There in the Manual: They're never referred to as "Three Blind Mice" in the film. See No Name Given below.
- Blind Black Guy: What they pretend to be.
- Disney Villain Death: Bond outsmarts them during the Car Chase, and they drive over a cliff to their deaths. Their hearse catches fire and explodes for no reason while falling.
- Every Car Is a Pinto: Their hearse catches fire for no apparent reason when they fall down after Bond outmaneuvers them.
- Hollywood Silencer: Complete with soft "fwip" noises when they shoot Strangways.
- In the Back: They attack Strangways in the back.
- Ironic Nursery Tune: A calypso version of "Three Blind Mice" accompanies their appearance.
- No Name Given: Their names are not mentioned in the film. They are nicknamed "The Three Blind Mice" because a calypso song of the same name plays when they appear on screen for the first time. Plus, they're a trio and pretend to be blind.
- Obfuscating Disability: They pretend to be blind for cover.
- Professional Killer: All three are professional killers on Dr. No's payroll.
- Sinister Shades: As part of the blind disguise.
- Soundtrack Dissonance: They have become known as "The Three Blind Mice" because of the calypso song that accompanies them during the film's opening sequence. Said song doesn't sound particularly evil.
- Terrible Trio: And they use their number to ensure their chances, see below.
- There Is No Kill Like Over Kill:
- They all shoot Strangways at the same time with several bullets, leaving him no chance.
- This also applies to their own deaths. When Bond is able to maneuver their hearse off the road, it explodes for no reason just to let the viewer know they made it to "their funeral".
Played by: Reggie Carter
A henchman of Dr. No. He is ordered to intercept Bond at the airport in Kingston and passes as a driver sent by the governor.
- Better to Die than Be Killed: Once discovered by Bond, he commits suicide by a Cyanide Pill cigarette rather face interrogation.
- Mooks: He is the first henchman that Bond encounters in the entire film franchise. Bond gets the jump on him first, but Jones tricks Bond into giving him his cyanide-filled cigarette, which he promptly bites down on, saying, "To hell with you" as his last words.
- Not My Driver: Bond gets suspicious of Jones pretty quickly actually, calls the British governor of Jamaica and finds out nobody has been sent to pick him up at the airport.