Ever since they solved their first case on Halloween, Fred, Daphne, Velma, Shaggy, and Scooby have formed an independent mystery solving group.
- Amateur Sleuth: They started out as freelance young detectives solving mysteries, after finding some enjoyment busting Mr. Rigby. In the present, however, the gang finally decides to try going professional.
- Five-Man Band: The group makes up one if Scooby is included. However, their roles as stated in the movie aren't the traditional ones.
- The Big Guy: Fred is called the muscle.
- The Face: Daphne is described as being good with people.
- The Smart Guy: Velma, as usual, is the brains of the operation.
- The Heart: At the end of the film, they realize Shaggy and Scooby are the ones to bring them all together.
- Team Pet: Scooby, who never gets a role defined for himself outside of sharing being The Heart with Shaggy.
- Two Girls to a Team: Daphne and Velma.
- You Meddling Kids: As per tradition, they're on the receiving end twice in the film:
- Their first-ever case leads to the arrest of Mr. Rigby, who promptly calls them out as such.
- Dick Dastardly, the film's primary antagonist, finally utters this after being unmasked twice by the gang.
Voiced by: Frank Welker
Shaggy and Mystery Inc.'s trusted, yet fearful, Great Dane. He's known Shaggy since they were kids and has promised to always be his best friend.
- Adaptational Backstory Change: This version of Scooby used to be a nameless stray in Venice, California before being rescued by Shaggy when an officer tried to impound him. He's also a descendant of Alexander the Great's own dog, Peritas.
- The Heart: By the end of the film, Velma finally assesses that this is the role he shares with Shaggy within Mystery Inc.
- Team Pet: Serves as the team's sleuthing dog.
Voiced by: Will Forte; Iain Armitage (Young)
Scooby-Doo's inseparable best friend since childhood, and the easy-going member of Mystery Inc. He's known for his bottomless and unusual appetite, along with his quick-footed cowardly nature whenever danger arises.
- Green-Eyed Monster: Shaggy grows increasingly jealous of Scooby's newfound importance, though it's seemingly because it threatens to break down his tight friendship with him.
- The Heart: By the end of the film, Velma finally assesses that this is the role he shares with Scooby-Doo within Mystery Inc.
- Hidden Depths: When the idea that Scooby will outgrow him starts to sink in, Blue Falcon tries (and fails) to give him a pep talk. Shaggy gives one right back, psychoanalyzing him - mentioning his Inferiority Superiority Complex, living up to his father's massive legend and the Imposter Syndrome that comes with it - to the point where it visually shakes him.
Voiced by: Zac Efron; Pierce Gagnon (Young)
The de facto leader of Mystery Inc. Fred Jones believes he's the man with the plan, with the help of his trusty and cherished Mystery Machine.
- Adaptational Comic Relief: In the B-Plot with Fred, Daphne, and Velma trying to locate the missing Shaggy and Scooby, Fred generally serves this role with his oblivious nature.
- Adaptational Dumbass: Compared to the co-running Scooby-Doo and Guess Who?'s version, Fred in this universe leans towards the dimmer interpretation seen in Mystery Incorporated.
- The Big Guy: Is described by Simon Cowell as the muscle, which Fred is fine with as he usually has a gung-ho attitude in most situations.
- Drives Like Crazy: A fairly competent driver, but he has his moments of recklessness like making an impulsive and illegal U-turn to reach the Takamoto Bowl, or taking his eyes and hands off to mock Velma.
- Dumb Blonde: This version of Fred leans towards a Book Dumb jock type, getting lost in Velma's Techno Babble or wooed by Officer Jaffe on looks alone. Though he does have his moments of quick wit, such as smuggling away the instructional scripture from Dastardly's ship which details how to close the Underworld's gates.
- In-Universe Nickname: According to Scooby's own words on the SCOOB! website, he's known as "Mr. Responsible."
Voiced by: Amanda Seyfried; Mckenna Grace (Young)
Daphne serves as the team's charismatic ace communicator. She's claimed to have an efficient sense of social dynamics and connection within her own team, outsiders, and social media.
- All in the Manual: It's alluded to in the film by Simon Cowell, but Daphne's liaison skills are elaborated on a little more on the SCOOB! movie website.
- The Face: Daphine is described as being good with people.
- The Chick: Not as much as previous versions, but she is still this for the team.
- Crazy-Prepared: Daphne keeps a spare Scooby Snack in her boot for emergencies, and a spare-spare in her other boot, in case Scooby eats the first one.
Voiced by: Gina Rodriguez; Ariana Greenblatt (Young)
Velma serves as the leading brain of Mystery Inc's Evidence and Research branch. She's always the smartest person in the room, which can make her socially awkward around some folks, but this also enhances her brand of humor and charm.
- Adaptational Attractiveness: Similar to the live-action films, Velma's nerdy demeanor is still present but she's nowhere near as frumpy looking as some of her prior animated incarnations.
- Adaptational Nationality: Due to her Ambiguously Brown skin and use of Gratuitous Spanish, it's implied she may be Latina in this version.
- All in the Manual: While her general role is alluded to by Simon Cowell, the film's website specifically cites that Velma is responsible for handling Evidence and Research in the team.
- Ambiguously Brown: In this incarnation, Velma has received a bit of a tan on her complexion.
- Blind Without 'Em: A scene in the credits shows her struggling to find her fallen glasses.
- Dark-Skinned Redhead: Due to her aforementioned new trait, she falls under this trope.
- Deadpan Snarker: Her recent trend towards this trope is alive and well in this incarnation.
- Gratuitous Spanish: Says that Dick's Rottens are increíble while examining one of them.
Falcon Fury (later known as the Falcon Force)
Voiced by: Mark Wahlberg
A rather dimwitted superhero who is seeking to stop Dick Dastardly.
- Canon Foreigner: He doesn't originate from Dynomutt, Dog Wonder.
- Legacy Character: He's taken over the mantle of Blue Falcon from his father, the original Blue Falcon.
- Hidden Depths: Turns out he knows he's not as awesome as his dad used to be.
- Manchild: Despite being an adult he's considerably immature, having a massive Totally Radical attitude and having designed the Falcon Fury to rain balloons whenever he says his name in his intro. Dynomutt even calls him a "large adult son."
Voiced by: Ken Jeong
Blue Falcon's faithful sidekick, the Dog Wonder.
- Adaptational Intelligence: Unlike in Dynomutt, Dog Wonder, he's far more intelligent.
- Covert Pervert: Implied when he thinks Velma will hack into his search history.
- Cute Machines: Maybe not to the extent of Dick's minions, but his expressiveness and happy facial expressions means he can shift from cool badass to adorable canine companion on a dime.
- Did You Think I Can't Feel?: He is legitimately upset when Brian dresses Scooby up in his own costume.
- Deadpan Snarker: He has quite a dry wit in this film.
- Divergent Character Evolution: To set him apart from Scooby, whom he had numerous similarities to in other incarnations, this version is smarter and snarkier and has a more complex design that plays up his Robot Dog look.
- Emerald Power: His color scheme is partially green, and he's shown to be a physically powerful and determined cyborg dog though his powerset.
- Foil: He is much more heroic and stoic, in comparison to the Lovable Coward Scooby, and is originally much more distant from his master in contrast to Scooby and Shaggy's closeness.
- Hypercompetent Sidekick: In a role reversal of the usual Blue Falcon and Dynomutt relationship, this Dog Wonder tries to keep Blue Falcon in line from his own goofy antics. He eventually learns to accept this role towards his original partner's son.
- Jerk with a Heart of Gold: He may seem bitter and snarky, but he's actually really sweet once you get past his grumpy exterior or when he's off duty. He just takes time to warm up to a new partner.
- Made of Iron: Easily shrugs off both being blown up in the Mystery Machine's crash landing and thrown around by Cerberus like a ragdoll without so much as a scratch.
- Not So Above It All: Despite his mature professional status, he does get a few funny moments, like getting hacked into and revealing his history.
- Robot Dog
Voiced by: Kiersey Clemons
Blue Falcon's human sidekick.
- Adaptation Deviation: She isn't normally associated with the Dynomutt, Dog Wonder cast, as she's typically the team leader of the Teen Angels. Though it's implied in the epilogue she's working with Captain Caveman, who's one of the new recruits of the Falcon Force.
- Black and Nerdy: She's a hair-bun wearing African American pilot with great navigation skills.
- Catsuit: She wears a skin-tight suit throughout the whole movie.
- Deadpan Snarker: She mirrors Velma to an extent on this trait, sarcastically pointing out the tropes of Dastardly's plan and commenting on her shock of Brian not figuring out a thinly veiled trap.
- Genius Bruiser: She's shown to be an excellent pilot and navigator, quickly outmaneuvering and outsmarting Dastardly's Mean Machine with enough time. Plus, in a last-minute combat scenario against Captain Caveman, she was the only one who could hold her own.
- Hartman Hips: Her catsuit does show off her curvaceous body.
- Hypercompetent Sidekick: To Blue Falcon, usually the one to do the research with Dynomutt instead of Brian.
- The Pilot: She drives the Falcon's airship on a motorcycle-like interface.
- Prim and Proper Bun: Her preferred hairstyle, which conveniently goes well with her straight-laced and strategic personality.
Voiced by: Jason Isaacs; Christina Hendricks (disguise)
- Adaptational Attractiveness: He has a huge, muscular build in this film. Isaacs specifically states Dastardly has "a giant simian jaw and a huge kind of Mr. Incredible body." Downplayed, however, since other characters still point out how ugly he is with his comically large nose and protruding chin, much to his chagrin.
- Adaptational Badass: In addition to his aforementioned physique, he's described by Isaacs as "slightly more of a force."
- A Dick in Name: But of course! Affably Evil as he may be, Dick is still the same old foul, dastardly cad he's always been.
- Badass Baritone: Jason Isaacs gives him an especially deep, authoritarian voice.
- Badass Longcoat: Dick comes with his signature purple trench coat as always. Only this time, he actually does have the badass credence to go with it.
- Big Bad: The central antagonist of the film, due to him risking the apocalypse just to bring back Muttley.
- Cool Car: His racing days may be over, but Dick still has his trusty old Mean Machine stored in his lair somewhere, awaiting the day he'll need its service again. And he does, following his imprisonment at the film's conclusion. Muttley busts him out by ramming the car through Dick's jail cell.
- Dastardly Whiplash: But of course; it's right there in his name. He's a Card-Carrying Villain, and even if he's more Affably Evil than he normally is, he's just as nasty as he was in Wacky Races. He's trying to open the gates to the Underworld, dresses all in dark colors, and he's got the handlebar mustache covered.
- Even Evil Has Loved Ones: As usual, for how despicable he is, he cares deeply for Muttley. In fact, part of the reason he needed to personally open the Underworld's gates was to find the trapped Muttley.
- Evil Brit: Comes with being voiced by Jason Isaacs.
- Evil Counterpart: To Shaggy, honestly, since like Shaggy, a number of actions are influenced by his love for his dog.
- Kick the Dog: Instead of Muttley, he punishes one of his robot minions to show how evil he is.
- Master of Disguise: He wears Latex Perfection masks, and is able to perfectly imitate the voice and body of whoever's face he wears. This includes Officer Jaffe, a police officer of the opposite sex and many years his junior.
- Moral Myopia: While he goes to great lengths to get his dog Muttley back, he has no problem taking Scooby away from Shaggy, or trying to kill the latter and the rest of Scooby's friends in order to do so.
- Non-Standard Character Design: In comparison with the semi-realistic humans in the film, Dastardly stands out with his big chin and pointy nose, giving him an overall cartoony look like his Wacky Races counterpart.
- Pet the Dog: Genuinely cares for his dog Muttley, even willing to risk his life in order to save Muttley from the Underworld. He even felt regretful upon summoning Cerberus after opening the Underworld's gates; even advising Scooby and the other people (or Scooby at least) to run away to avoid getting attacked by the hellhound.Dastardly: [intimidated by Cerberus' appearance] Uh oh! Little more than I bargained for. [to Scoob] If I were you, I'd RUUUUUUUN!!!!
- Purple Is Powerful: Dresses in his signature purple trench coat, and he's much more capable here.
- Rogues Gallery Transplant: He was the main antagonist of Wacky Races and occasionally went up against Yogi Bear, but goes up against Mystery Inc. and the Falcon Fury crew in this film.
- Unreliable Narrator: The way he recounts the whereabouts of his old friend Muttley. His narration tries to make him sound more sympathetic in accidentally trapping Muttley in the Underworld, claiming that Muttley's desire to enter the portal to there overwhelmed the dog's reasoning against the dangerous idea. The corresponding flashback contradicts this lie, as Dastardly was the one who forced him to go first despite Muttley's visible reluctance.
- Villainous Friendship: Has one with Muttley.
Voiced by: Billy West, Don Messick (laugh, archived audio)
- Sir Not-Appearing-in-This-Trailer: He remains absent from most of the marketing, even if he is usually seen with Dick Dastardly.
- Truer to the Text: Unlike his radically redesigned partner (and most of the movie's cast), Muttley looks extremely faithful to how he did in his original cartoons.
- Villainous Friendship: Has one with Dastardly.
Voiced by: Tony Cervone
Voiced by: Fred Tatasciore
Voiced by: Tracy Morgan
- Adaptational Backstory Change: Unlike in the cartoon where he was a frozen caveman, this one is contemporary, living in a Lost World with people like himself.
- Adaptational Intelligence: He comes off as more casually verbose compared to his Hulk Speak original self.
- Advertised Extra: Promotional posters and other tie-in ads feature Captain Caveman triumphantly with Mystery Inc and the Falcon Fury crew. Yet, despite this film's plan to connect various Hanna-Barbera characters into prominence, he's only in a minuscule role of being a Hero Antagonist.
- Barbarian Hero: This version of him appears to have been designed with this in mind given his stockier, muscular build along with the presence of tribal tattoos and hair dreadlocks.
- Boisterous Bruiser: Has a LOT of fun fighting Blue Falcon and Scooby, and puts on quite the performance.
- Catchphrase: His iconic one, "CAPTAIN CA-AVE-MA-AN!", is used as a Battle Cry during his match with Blue Falcon and Scooby.
- Dreadlock Warrior: His hair and beard form dreadlocks, and he's the champion of his island and protector of the Cerberus' Skull.
- Hammerspace: His hollowed-out club can open up to reveal a cartoonish amount of dangerous, oversized weapons.
- Hero Antagonist: He fights (and curb stomps) the protagonists because they want to recover the skull of Cerberus of which he is guardian. But in the epilogue, he's one of the new recruits to the Falcon Force.
- Heroic Build: He's a superhero who, if his arms are any indication, is absolutely jacked underneath that hair.
- Pintsized Powerhouse: Sure, he's very short for a caveman, but as Brian painfully finds out, much stronger than he looks.