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Beauty Mark: Next to his left eye. Considering his terrible luck, the Japanese connotations definitely fit.
Be Careful What You Wish For: The symbiote suit takes Peter's body out to fight the Sinister Six after he wishes aloud that he could just fall asleep and find them in jail in the morning.
Berserk Button: Try to hurt his loved ones and you'll be in for a world of pain. The best example, threatening Aunt May sets Spidey off so much that he's able to drive off Venom. To clarify, he can almost never match Venom in a fair fight, except when Venom tries to go after Aunt May.
Attacking Gwen or any of his other friends is a bad idea, as well. Also, don't make him work with the man who shot his uncle, no matter how remorseful that man is.
Betty and Veronica: Gwen fills the role of Betty, but curiously enough, the role of Veronica is zigzagged between his other love interests at different times. This would include Mary-Jane (VERY short-lived in this adaptation, due to its cancellation), Black Cat, Liz and, ironically, Betty Brant.
Blindfolded Vision: As a tactic against Mysterio's illusions, correctly predicting that his spider-sense would only kick in for actual danger.
I Just Want to Be Normal: He has a few bouts of this due to the responsibilities that come with his powers. Not to mention the fact that the inflated ego resulting from said powers directly resulted in Uncle Ben's death, which he still blames himself for. After the Lizard arc he keeps a vial of Doc Connors's gene cleanser just in case he decides to go normal forever until he realizes that his powers are necessary end of the first season and throws the solution away.
I Let Gwen Stacy Die: Another part of his history that we never got to see due to the show's cancellation...except that Word of God didn't intend to go for it in this adaptation anyway.
Lethal Chef: He decides to prepare Thanksgiving dinner so Aunt May can recover from her heart attack. His attempts result in a burned cream sauce and a bunch of exploded yams.
Lightning Bruiser: The guy has taken hits that would have killed most fragile speedsters. He's survived vibro-blasts that tear through concrete walls, multiple grenades to the face, has fast enough reflexes to dodge lasers and lightning from Electro, and been hit so many times by villains with super-strength that if a contest was made on drinking a bottle of beer for every time he gets hit, the person's liver would be wrecked by half the first season.
Green-Eyed Monster: Toward Peter, as usual. Though he is less aggressive about it than most other versions of the character. Peter still is aware of it and tries to avoid it so Harry will end up better, but this doesn't end well.
Shrinking Violet: A rare male example in the first few episodes, where despite (or perhaps because of) his status he is very shy, withdrawn and lacking in confidence, only opening up to his closest friends. Even when in his more jerkassy moods when under the influence of the Green he is still softspoken and awkward, and it tinges on Softspoken Sadist when he shows his Manipulative Bastard side in the finale.
Took a Level in Badass: When he's back from Europe he can fly a helicopter and he actually saves Spider-Man's life at one point by destroying the windows and dispersing the gas the Goblin planned to kill Spider-Man with.
"Well Done, Son!" Guy: In spades. If you've seen the first movie, well this is taken up multiple notches. It's basically his goal for anything he does in the series. Getting a girl friend, doing well in school, being part of the popular crowd, ect. all seem to be an attempt to make Norman proud.
With Great Power Comes Great Insanity: His addiction to the Green did a major job on him, though luckily not as bad as it did on his father. This is a known side-effect of Globulin Green, and though it makes him stronger it also makes him prone to sudden bouts of rage. He undergoes clear Sanity Slippage in "The Uncertainly Principle," which includes flipping out and throwing his father into a wall, all while mood-swinging constantly, and even though he ultimately wasn't the Goblin, the show makes it very believable that he could have been if given the opportunity.
Eugene "Flash" Thompson
Voiced by: Joshua LeBar
Amazingly Embarrassing Parents: His mom manages to be this, inadvertantly humiliating him at his party by inviting Peter and revealing they were once friends.
Character Development: The second season saw Flash abandon much of his Jerk Jock characteristics in a bid to impress a girl he had a crush on. As a result, he also became less antagonistic towards Peter.
Heroic Bystander: At several points, he is willing to take risks to help Spider-Man, even once going around in a Spider-Man costume. He actually ends up saving Spidey from Venom at one point.
Hidden Depths: Turns out to hold sportsmanship higher than trophies.
Also, he is truly outraged when Spider-Man is framed for crime and refuses to believe it's the real one.
Jerkass Has a Point: Flash, of all people, calls Peter out for acting like a jerk to everyone when they were comforting about about his aunt being in the hospital. This made Peter realize the symbiote was controlling him.
Even Jerks Have Standards: She doesn't like Peter, but, after one dangerous incident, openly admits that she's glad he's alright and even hugs him out of relief, flat out telling him that while she might not like him, she doesn't want to see him hurt. Also, back toward the end of the first season, it's mentioned that "even Sally" is worried for Peter when she heard that Aunt May had a heart attack.
Makes a point of laying off Peter if Liz asks her to, showing that she at least genuinely respects Liz.
Jerkass: She has softer moments, but most of the time, she's this. She even acts like one to Flash, and that's prior his Character Development.
Ship Sinking: Very against Liz/Peter. She got her wish when Liz broke up with Peter (but it was actually Peter who broke her up).
Spared by the Adaptation: Although considering that her comics counterpart died as consequence for trying to get pictures of Spider-Man, and her animated self had no love even for him, the only thing similar with this Sally is her Alpha Bitch nature.
All Guys Want Cheerleaders: Is a cheerleader and Peter's first girlfriend on the show, though Peter later decides that he does not, in fact, want a cheerleader.
Defrosting Ice Queen: When first introduced, she is in a relationship with Flash, sees Peter as a geek and is extremely dismissive toward him, almost as much as Sally. She, however, quickly comes to appreciate him and ends up being the first girl to actually date him.
Promoted to Love Interest: Inverted; this is the first Spider-Man animated series where she is not Peter's main love interest, although according to Word of God she would have become the main girl if the show had had a chance to progress.
The Rival: Subverted; she appears to be this to Gwen at first, but it's quickly made clear that she only went to the prom with Peter to help and won't get in the way of Gwen. She's more of a rival to Liz, due to encouraging Peter and Gwen to being a couple.
Romantic False Lead: Intentional, her date with Peter really was just a one time thing. She does like him as a friend, but if anything, she would rather see him with Gwen.
Big Bad: Of Season 2 and arguably the whole show. Due to the fact that he was working with Tombstone to make supervillains and was responsible for Otto Octavius Jumping Off the Slippery Slope, he's got some connection to every major villain except for Venom.
Even Evil Has Loved Ones: For all his evilness, he does shows concern for Harry when he and Spider-Man discovers he is the Green Goblin.
Horribly twisted in the season 2 finale, where it turns out he had been framing his own son when he was the real Green Goblin; he did so to "protect" him, because he didn't want to go to jail and be unable to make his son a man.
Villain with Good Publicity: Most people just see as a perfectly respectable businessman. Behind the scene, he agrees to make a deal with the Big Man to create new supervillains just to keep Spider-Man busy.
Good Is Not Nice: Most notably, when Rhino asks for Peter, JJJ notices Peter and gestures for him to hide, then lies to Rhino - claiming that he's never met Peter, that everything is done through email - even though this could easily cost him his life.
Jerk with a Heart of Gold: When Aunt May has a heart attack, he feels it's his responsibility as Peter's boss to break the news to him. It backfires, but that's not his fault. We also see that he's extremely affectionate towards his son.
Not only that, but when Rhino grabs him and threatens him to tell where Peter is, he actually sees Peter behind the Rhino, secretly indicates that he should hide, and lies right to Rhino's face about not even knowing what he looked like. The guy may not be the nicest guy, but never call him a bad guy.
Genre Savvy: One of the few characters to never fall for the hero being framed trick. It happens twice in the show, and both time he is able to say this isn't the real Spider-Man through logical arguments.
Karma Houdini: He is responsible for mutating Kraven, stole Dr. Connors' research and caused him to leave through blackmail about him being the Lizard, amongst many other things. He gets away with it at the end thanks to the show's cancellation.
Anti-Villain: In his first appearance; he only wanted revenge on Norman Osborn for stealing his tech flight idea, which is, admittedly, a perfectly acceptable justification. Of course, after his crimes, he's forced to keep going that way.
Small Role, Big Impact: Word of God states that Norman Osborn, not wanting to ever feel helpless again, began using Globulin Green on himself in response to the Vulture's attacks. Without the Vulture to provoke him, Norman probably wouldn't have been able to claim the role as the show's Big Bad.
Starter Villain: The first actual super-villain Spider-Man fought. Also the first one, with Electro and Lizard, who motivated the Big Man into asking Norman Osborn to create new villains for him.
Taught by Experience: In his first episode, Spider-Man defeats him by destroying his harness through the use of Super Strength. He learns from it, and has his harness reinforced with a stronger steel when he comes back in the Sinister Six.
Smug Snake: Becomes one after switching from The Dragon to The Starscream - he's just not as good at scheming on his own as he is at carrying out someone else's schemes which leads to his becoming a pawn of the Green Goblin.
The Starscream: During the gang war arc. Which is surprising because he seemed to be extremely faithful toward his leader. However, he is understandably fired by said boss shortly after they (or at least the boss) were arrested.
Unwitting Pawn: His scheme to betray Tombstone turns to have been all a plan orchestrated by the Goblin using him.
Use Your Head: Though he doesn't really start to do it until season 2.
You Have Failed Me: Too many times with Tombstone, and is fed up with him. This results in Hammerhead becoming The Starscream.
Blessed with Suck: He got highly powerful electrical powers... so much that he is forced to wear a suit constantly to prevent them from destroying everything around him. To make things worse, he can't even drink anymore since the mere contact with his mouth will cause a shock.
Breath Weapon: In the Engineering arc he starts shooting lightning from his mouth.
Comes Great Insanity: When first introduced, he appears to be a fairly nice and sane guy. The accident that gave him power clearly caused him to snap completely.
The fact that everyone was misunderstanding to him probably factors in.
Adaptational Villainy: While still a villain, in the comics Connors' Lizard persona is reluctant to attack his own family. This version of the Lizard on the other hand is completely feral and has no hesitation trying to eat his son. At least, until Brand New Day, when comics!Lizard tried the same thing—and succeeded!
Anti-Villain: Type IV. He's a perfectly nice guy, it's just that there's this one time he turned into a mindless, vicious animal, and no one is ever gonna let him forget it.
Freak Lab Accident: The experiment done on him was a failed attempt to give him an armor. It was later done correctly on the O'Hirn.
Heroic Sacrifice: Somewhat subverted, in that while the act really was a heroic self-sacrifice that ended in his disintegration, the episode in question uses The End... Or Is It?, as after things seem to have ended, he's shown reforming and then blowing away on the wind, so he's really Not Quite Dead.
Story-Breaker Power: Getting there by "First Steps", once he's started to get a proper hang of his abilities. It's probably no coincidence that his last appearance in the show is in the episode where he absorbs Rockaway Beach. Yes, all of it.
That Man Is Dead: He doesn't take it as far as Electro, but on a few occasions he and Spidey seem to refer to "Flint Marko" like he was a totally separate person to emphasize this. He mostly seems to do it to be dramatic, though, and unlike Electro he doesn't seem to mind being called Marko or cast of his old identity that much.
Demoted to Extra: Happens to him after the Green Goblin arc of season 1 and up until the episode "Gangland". In the first episode of the series he sends Enforcers to destroy Spider-Man and is set up as New York's most powerful crime lord. He becomes the Big Bad during episodes 4-6, ordering Norman Osborn to create supervillains to distract Spider-Man and is set up as one of Spidey's arch-enemies. He also plays a big role in the Green Goblin arc (7-9), fighting against Green Goblin and teaming up with Spider-Man to stop him. However after that, he becomes a secondary character and in the Symbiote arc he only appears briefly in beginning of episodes 12 and 13 and only accepting job offers and nothing more. Thus, Symbiote/Venom replaces him as Big Bad of Season 1. In the first half of season 2 (Master Planner and Venom arcs) he doesn't even appear and is only mentioned in "First Steps". In the Gang War arc, while he is set up as one of the crime lords fighting for control over New York, he doesn't appear in Accomplices and appears in the beginning of "Probable Cause". However in "Gangland" he returns as one of the big bads and fights against Doc Ock, Silvermane, and Spidey. In the final episodes he doesn't get mentioned at all.
Enemy Mine: With Spider-Man against the Green Goblin.
Even Evil Has Standards: Stays behind to help Spider-Man search for a bomb in his high-rise, answering Spidey's questioning with "My party, my mess." Also when Tombstone gives a symbiote-influenced Spider-Man his "fight no ordinary crime for a week" employment test, he notes that Spider-Man can fight any supervillain crime that directly endangers innocent people.
Noble Demon: Only shows this when up against The Green Goblin, but he's the least evil of the three Big Bads (the two other being Green Goblin and Doc Ock); whereas Ock and Gobby are psychos with megalomaniac purposes, he's only interested in profit and doesn't seem too fond of gratuitous violence. He even admits to Spider-Man that he has nothing against his heroic acts and only sees him as a problem because he's causing problems to his own association.
We Can Rule Together: When first meeting Spider-Man in person, he offers him to pay him if he agrees to only chase criminals when allowed by him to do so. Of course, Spider-Man declines. He later does agree under the symbiote's influence, but goes back to declining it after being freed from it.
Cast as a Mask: Steve Blum doesn't voice any of his potential secret identities.
The Chessmaster: Most of the season 2 arcs are part of his plan to take over as the new Big Man and get rid of Spider-Man.
Crazy-Prepared: Seriously, he seems to have a back-up plan, trick or trap placed in advance for any situation. In fact, at several points where Spider-Man has been close to catch him, it turned out he had already put something (typically Bombs) to distract him long enough to escape should this happen.
Rhymes on a Dime: While his isn't usually an example, he does this in "Opening Night". Not only is it lampshaded, but it's also partially justified: several of his lines are quotes from Shakespeare's verse. It also acts as a clue to his identity: Harry Osborn, the prime suspect behind the mask, was supposed to be playing Puck in a school play, and all of the Shakespeare lines are Puck quotes. Turned out to be a Red Herring, but nice touch...
You Fight Like a Cow: Unlike most of Spidey's villains, Goblin doesn't get annoyed by the Web-Head's quipping and mocking. In fact, he quips right back, and he does it pretty dang well too. Spider-Man even lampshades this.
(While Green Goblin and Spider-Man are battling all over town)
Goblin: Well you know the old saying: If you can't join 'em, beat 'em!
Spidey: Took the words right out of my mouth. Is that a felony?
Goblin: Oh, I should hope so. This little goblin wouldn't be caught dead committing a misdemeanor!
Spidey: Oooh, nice banter! Your aim could use a little work though.
Goblin: Well, practice makes perfect!
Spidey: Keep telling yourself that!
Doctor Octopus/Master Planner (Otto Octavius)
"So, any last words, Arachnid?"
Voiced by: Peter MacNicol
Adorkable: As Otto Octavius, prior to his accident.
Benevolent Boss / Bad Boss: Has shades of both. On one hand, he generally treats his minions with respect and even friendliness. On the other, he doesn't go out of his way to ensure their safety when things are going seriously wrong, as demonstrated when he leaves Electro behind to die in his self-destructing Evil Lair.
Beware the Nice Ones: When first introduced in the show, Dr Octavius is a nice, shy, awkward guy who could be considered as Oscorp's Token Good Teammate, often expressing worrying about his coworkers' well-being. After go crazy and becoming Dr Octopus, though, he becomes a fearsome ChessmasterEvil Genius who 's responsible for most of the Villain Team-Up in the show and becomes one of the top criminal leaders in the town.
Extreme Doormat: Gets pushed around quite a bit before his transformation occurs.
Even Evil Has Standards: Doc Ock politely asks and waits for Aunt May and Anna Watson to excuse themselves from the upcoming brawl between the Sinister Six and Spidey, even halting Rhino as he tries to charge through them.
The Starscream: Sort of; he always had a repressed resentment toward Norman Obsorn, but was too shy and insecure to actually act upon it. This... doesn't turns out well when he gets crazy enough to do it.
The Unfettered: According to Word of God, the robot arms didn't drive him crazy. Their power and the events surrounding his gaining them simply drove him to lose his inhibitions.
Villainous Friendship: Seems to have one with Adrian Toomes/the Vulture, whom he knew before either of them became criminals. He also tends to be on friendly terms with Electro, but evidently not enough to go out of his way to save his life.
Venom (Eddie Brock)
"We're poison to Peter Parker and Spider-Man; WE'RE VENOM!"
Big Bad: Of Season 1 (along with Tombstone) and first half of season 2. In Season 1, aside from Peter's Character Development, Brock's increasing anger towards Peter and his eventual Face-Heel Turn was one of the main plots of the season and eventually he turned into Venom in the final episodes of first season. And during the first half of season 2, Peter was mainly concerned about him. Also, his actions made Peter realize that he loves Gwen most and thus sets the Season 2 love triangle plot in motion.
Composite Character: His Eddie Brock self shares characteristics with the Ultimate version of the character, being a scientist and Peter's close childhood friend, though he also has the musulcar build of his mainstream self and the symbiote retains the alien origin from the mainstream version.
Conspicuous CG: The symbiote's first appearance. It's not the usual 3D model example, but is instead a black blob whose shine doesn't at all conform to the curve of the space shuttle's hull. It's like an awkward piece of clipart.
Death Seeker: According to Word of God, his frequent reckless heroics in earlier episodes were a sign of this on a subconscious level.
Evil Counterpart: Comes with being Venom. This dynamic is also explicitly noted with their backstories; while Peter and Eddie lost their parents in the same accident, Peter was able to rely on the guidance of his aunt and uncle to eventually become Spider-Man. Whereas Eddie's increasing resentment made him the perfect host for Venom.
Hidden Depths: Eddie always resented Peter for having a better life than he did.
Hypocrite: Accused Peter of being self-centered and using everyone around him to get what he wants and not caring who he hurts, regardless of how close he is to them. He then started to do the very same things as Venom.
It's All About Me: Peter took photos of Dr. Connors as the Lizard? He's betraying Eddie's trust in him! The Connors have low funding and are forced to drop Eddie from their payroll? But how will Eddie pay for college?! Spider-Man's destroying the Symbiote because it's dangerous and evil? But that was Eddie's last chance at keeping his internship! And so forth. And all of this was from before he became Venom.
The Nth Doctor: The symbiote uses an evil version of the voice of whoever it's bonded to. When it's engaged with Peter in a Battle in the Center of the Mind, it's played by Josh Keaton, using an... eviler version of Spidey's voice. When bonded to Eddie, it's voiced by Ben Diskin in a high-pitched monstrous voice alongside Eddie's normal voice.
Rival Turned Evil: Though more like best friend turned evil, since he wasn't especially in rivalry with Peter at the beginning.
Took a Level in Jerkass: About the time Peter got the black costume and even more so after he started wearing it. In the case of the former, it's because Peter accidentally stealing the suit lead to Eddie's whole life falling apart.
Top-Heavy Guy: Eddie has this going on, but it's pretty exaggerated with Venom.
Voice of the Legion: Venom speaks in two voices simultaneously; Eddie Brock's normal voice, combined with a more twisted and inhuman voice by the same voice actor. And the two voices aren't always even at the same pace, making it both brilliant and terrifying.
Predecessor Villain: He was the main criminal leader twelve years ago until Frederick Foswell exposed his activities, causing him to end up in jail while his empire frailed and was taken over by Tombstone.
Chrome Champion: A villainous example. Also, unlike his comic book's version, Spider-Man's webbing can stick to his skin if Spidey had cooled him down previously.
Composite Character: His gambling problem and being a biological brother to Liz Allan (in the comics, his last name was Raxton and he and Liz were stepsiblings) comes from Bennett Brant (Betty Brant's brother), while his powers and first name comes the comics version of Molten Man.
Fatal Flaw: His gambling addiction is what causes him to become a meta-human against his will.
Flaw Exploitation: He'll easily stab himself in the proverbial foot if you dangle a good enough prize ahead of him, constantly looks for easy ways out of his problems and doesn't have enough common sense to quit while he's behind. This makes him very gullible - something that's taken advantage of by Gaxton, The Goblin and Spider-Man - who humiliates him twice merely by goading him with a simple "I bet you can't-".
Ineffectual Sympathetic Villain: Not only is his heart really not in it, but as Goblin says he's basically an amateur forced to play supervillain. The first time he appears as Molten Man his every action just makes things worse for himself, and once Spider-Man is forced to fight seriously he gets humiliated in seconds. The second time he's coerced into fighting in the first place, gets pushed around by the other villains and is tricked once again. Even without his tragic backstory, it's not hard to feel sorry for the guy.
Magic Pants: When his powers activate, it burns off all his clothes...except for his underwear, apparently. At one point his pants seem to reappear after his powers are turned back off.
Adaptational Badass: In the comics, Rhino's a big dumb brute Spidey beats on a regular basis. Here, he's an unstoppable force of pure destruction that Spidey can only defeat through cleverly exploiting his one real weakness, and as seen in "Accomplices", can occasionally pull one over the web-slinger with some effort.
Anti-Villain: Type I. He's mean and reckless, but he has standards. Completely self-serving standards, but still enabling him to do some good every once in a while.
Dumb Muscle: Sometimes. Occasionally will show flashes of insight: he's no genius, but he does make some fairly intelligent deductions, including being the first one to realize that if Peter Parker takes Spider-Man's pictures, he can use Peter to find Spider-Man. "I ain't stupid" is practically his Catch Phrase.
Faux Affably Evil: He speaks in a polite, somewhat aphoristic way which is enhanced by his strong Texan accent, but he's definitely not a nice person by any means (even outside of the whole "killing people for money" thing).
Wisdom from the Gutter: In his first episode, he speaks to Spider-Man about a man needing to follow his commitments and Spider-Man being one of his (i.e. it goes against his "code" to fail to kill a target). Peter later repeats this verbatim to Aunt May when offering to chip in to pay the bills.
The Blank: A variation: He has visible eyes and a mouth, just really no other facial features.
Cast as a Mask: Every one of his disguises uses the voice actor of that character.
Chekhov's Gunman: He's first introduced merely as a villain who impersonates Spider-Man so he can take advantage on his accusation of being a thief to steal money, and is defeated at the end of his episode (though he escapes). Norman Osborn recruits his service in the finale to pose as him while he's the Goblin, such giving himself an alibi. Also, he's revealed in the finale to have actually shown up before his first onscreen appearance.
Critical Research Failure: invoked He seems to think that the terms are "web-shooter" (as opposed to web-slinger) and "insect early warning system".
Foreshadowing: In his first onscreen appearance he disguises himself as Norman Osborn among several others. It turns out he'd been already been masquerading as Osborn a bit longer than anyone knew.
Actually a Doombot: The first time Spidey caught him, the Mysterio that was captured turned out to be a robot double. The second time, Spidey is smart enough to pinch Beck to make sure he caught the real one... turns out that this Beck was also a robot double.
Sissy Villain: Besides the costume and the accent as Mysterio, Beck out of costume is a wimpy actor with a bit of a lisp, and in his first appearance (before taking on the Mysterio identity), he's shown providing Room Disservice in a way (unintentionally?) evocative of Wint and Kidd in Diamonds Are Forever.
Science Versus Magic: Mysterio is introduced as an Evil Sorceror who looks down on technology and demands to worship him. But it's all an act and Mysterio's powers are purely technological.
Why Do You Keep Changing Jobs?: Initially assisting Chameleon, then serving as the Master Planner's dragon, and later commissioning suits for the Enforcers (who work under the Big Man). He might just be a freelancer not really loyal to any particular faction and is a criminal Gadgeteer Genius"for the Art" like his former partner Mysterio.
Kraven the Hunter (Sergei Kravinoff)
Voiced by: Eric Visbit
All Your Powers Combined: Kraven specifically asks for his DNA getting combined with African big cats`, granting him the speed of a cheetah, the agility of a leopard and the strength of a lion.