In Magi - Labyrinth of Magic, Ka Koubun's attempt to make King Sinbad marry Princess Kougyoku, by making it look like Sinbad had violated her, fails, because his henchmen can't stand seeing their princess crying and so sad.
In Gakuen Babysitters, Chairman Morinomiya, who usually demands for Ryuuichi to work hard and to keep a punctual schedule, states this about herself when posed with the option of waking him up while he's sick.
In Naruto, anyone who has met Uchiha Madara and has spoken to him pretty much hates his guts, barring his brother Izuna, his former best friend Hashirama, and his protege Tobi. Not even Kurama can stand him, and considering the shit he's probably had to put up with over the last few centuries, that says a lot. The people that can stand him? Izuna's dead, and Hashirama became his mortal enemy the moment Madara decided to attack Konoha, the village they built together. Even his long-time accomplice Tobi, aka Obito, reveals that he doesn't care for him all that much either.
There's also Sasuke himself during the early episodes. Even before he and Naruto actually become friends, he is absolutely furious at Sakura when she confides in him that she believes Naruto is such an undisciplined prankster because he had no parents to teach him how to act and promptly chews her out for it. After all, he knows from personal experience that not having parents bites.
In Bakuman。, Eiji Niizuma follows his rivals' work so regularly that his refusal to read Tanto is treated as an OOC Is Serious Business moment that indicates that he thinks they're not living up to their potential.
A Show Within a Show case becomes a plot point in and out of that universe. Mashiro and Takagi's Perfect Crime Party, a group of elementary school students who do harmless pranks called "perfect crimes", don't do anything that could cause trouble for others; they suggest that if they broke into a bank vault and left behind a note without stealing anything, the security company's reputation would suffer. In response to someone imitating PCP by breaking into a bank vault and leaving a note behind, Takagi writes a story in which Akechi, The Rival to PCP, tells some copycats that he knew it wasn't PCP because they wouldn't do anything like that, and ends with PCP themselves commenting about how not everyone can do things the way they do.
In Death Note, L and Near are not paragons of justice, and only take on cases that they're interested in. However, they both disapprove of what Kira is doing and the mindset behind it, with Near having an especially strong hatred of Kira's followers.
In One Piece, the Straw Hats, for the most part, care about their members and friends more than anyone else, even the entire rest of the world, in Robin's case. However, they're typically shown to be quite upset whenever their enemies callously harm their own minions, from Sanji and Franky's shock at Rob Lucci pulling a You Have Failed Me on Nero, to Chopper saving an Enforcer from Gedatsu's attack.
As greedy as Nami is, she's willing to give up treasure for the sake of her friends, such as giving some of Thriller Bark's treasure to her friend Lola as a gift, or being willing to spend all the treasure to buy Caimie and set her free (unfortunately, she gets preemptively outbid).
Nodoka, in Saki, has repeatedly expressed that she doesn't believe in the existence of superstitions, even when her opponents make improbable draws right in front of her. In a flashback in Saki Achiga Hen, she hears her old friend Kuro telling her that because her Missing Mom told her to value the dora more, she has chosen to draw dora over completing her hand, and believes that as a result, she is more likely to draw dora tiles. Ako asks Nodoka if she's going to say her usual thing about superstitions, and Nodoka says she can't believe what Kuro is saying, but doesn't want to tell her off, either, possibly because she realizes the personal significance of it to Kuro.
In Nana when porn actress Yuri Kousaka reveals that her real name is Asami Matsumoto, she comments, "I may be a shameless woman, but even I don't make porn flicks under my real name."
In Is This a Zombie?, Orito is a Casanova Wannabe who's always shamelessly hitting on girls, trying to peep on them, etc. When Ayumu gets possessed by Belphegor, he makes offensive Straw Misogynist remarks. Orito is horrified and quickly declares that he does not believe in that line of thinking.
True, InuYasha is short-tempered, violent, and overall a bit of a prick, but there are plenty of lines that even he refuses to cross:
In the "The Tragic Love Song of Destiny" anime special, he refuses to attack Kikyo for the Shikon Jewel when she's badly injured and can't fight back, explaining to her that he doesn't "play dirty"; he even personally saves Kaede from Mistress Centipede while refusing to take her hostage for the jewel as Centipede intended to do.
When he fights Tōkajin, the latter demands that he eat some of the Ninmenka's fruit before their fight; given the source of the fruit, Inuyasha is quick to turn it down.
When he meets Shiori, he adamantly refuses to kill a fellow half-demon, let alone a little girl.
In Kage: While Jade is quite familiar with being a Combat Pragmatist and Guile Heroine, she is disgusted at the Guardians for framing Raythor and getting him banished to the Abyss of Shadows, feeling that there are some things a hero should not do, and that doing that to someone as honor-driven as Raythor is worse than killing him.
Ned Stark Lives: Tyrion Lannister has never gotten along well with Joffrey, who he considers an incredibly selfish and violent Royal Brat who would bring the Kingdoms down along with him; even so, he is disgusted and outraged when he hears that Joffrey didn't die from his fall into the Blackwater Bay at the Fall of King's Landing, but that he was slain in single combat by Stannis Baratheon, despite being crippled and half-conscious at the time. It's one of the few things he and Cersei can agree on by that point.
Molestia is very much the Anything That Moves type, as well doing quite a few that don't move, but she does have a few fetishes she doesn't engage in, such as vore or necrophilia, and won't violate those who are underage.
Raphael smacks Michelangelo on the head everytime the latter says or does something foolish, but even the former is disgusted by how poorly Neji treats Hinata
Similarly, Raph gets outraged when Itachi, while fighting Sasuke, tries to pull a Not So Different moment.
In Boys Und Sensha-do, Anchovy doesn't think much of Miho and is desperate to win against her to prove that her school's "not a joke", but she's horrified when Miho accidentally gets shot.
Colonel Yang from Halo The Art Of War is an eccentric Bunny-Ears Lawyer of an engineer and lenient with Spearhead's antics, but even he thinks Serena is too nuts to be allowed Spartan augmentation, and he also had Thom-293 taken off Noble for pushing his luck too many times.
For all the blatant cutesy-ness of Equestria and its inhabitants, they still consider that the pink heart-shaped Equestrian attorney badge is far too excesive in that regard.
Despite Trixie's Jerkass tendencies, and not being the most honest pony herself, she shows a dislike for liars that lie to save their own hide. This shows when she abandons her own witness after a very long string of lies, and later when she helps Phoenix to further expose another witness despite having already done so herself, which only serves to give an opening for Phoenix's case.
In The Dark Knight, Joker thinks he can prove this wrong, but it turns into a Double Subversion, when neither the boat of convicts, nor the boat of innocent people, go for his blackmail to have the people on one boat blow up the other, lest they both get blown up.
In The Distinguished Gentleman, a professional Con Man gets himself elected to Congress and discovers that he's small potatoes compared to the corruption that goes on in the Capitol. He then dedicates himself to exposing them.
In Felidae, Francis says this when Bluebeard comes to his house for the first time.
Francis: Did you come back here to take a leak? Forget it. You don't piss in here anymore. I live here now, and I have my standards.
In Predators, the benevolent doctor and one of the few good people on the team is thoroughly disgusted by Stans's "rapin' bitches" comment. He's later revealed to have probably been faking it — he's a serial killer himself.
Colonel Tavington in The Patriot inspires this among his countrymen. Cornwallis can be killed an honorable British General who respects both the laws of warfare and simply wants to reestablish British rule. He wants to maintain good if temporarily not peaceful relations with the colonials. However, he is still appalled at Tavington's brutal tactics and rightly faults him for the increasing resistance against the British from the American militia. Also, one of Tavington's men complains that there's no honor in burning down a church full of civilians, and is clearly reluctant to follow his orders to do so.
In The Little Mermaid, the Mermaid fails to win the prince's love, but can return to mermaid form if she kills him with a magic knife. She refuses (this helps her earn the chance to gain an immortal soul).
In Sourcery, Evil Vizier Abrim got rejected from Unseen University. They said he was mentally unstable. How do you manage to be too mentally unstable for Unseen University?
This idea is consistently overlooked by Safehold's Grand Inquisitor Zahspahr Clyntahn. As he piles on atrocity after atrocity in his attempts to crush the schismatic Empire of Charis, it never seems to occur to him that he's alienating the various clergymen and world leaders who should by all rights be his allies. Various characters do things like defect to Charis rather than face his telling them You Have Failed Me, or Mercy Kill enemy soldiers rather than take them alive to face the tortures of Clyntahn's Inqusition.
A rather dark take on this trope features in the later volumes of Harry Turtledove's Timeline-191 series. The prison camp guards are perfectly happy to keep African-Americans in a horrific, inhumane concentration camp, but lining them up and machine-gunning them in cold blood is too much; some are Driven to Suicide, others driven to drink and a few end up in asylums. This prompts the Nazi Expys to think up gas vans as an alternative that's easier for the rank and file to cope with. This is in fact based directly on what led the real Nazis to implement the gas chambers.
Patrick McLanahan from Dale Brown books is a major Military Maverick, but even he finds the National Guard pilots he's scouting out in Battle Born too lax and defiant for anyone's good. At least at first.
Harry Potter: Everyone in Hogwarts, be it Snape, Harry, or Filch, wanted Gilderoy Lockhart gone. It's believed that the only reason he got the job as DADA teacher is because no one else applied (the position is cursed — no one lasts longer than a year) besides Snape. And the only reason why the far more qualified Snape didn't get the job is because Dumbledore still needed him — not only for his plans against Voldemort, but also as the Potions teacher for the school.
The MST3K crew watched but refused to riff on this show as it was worse than Manos and the subject matter disgusted even them.
Community: Invoked Trope. The last security guard at Greendale, who is willing to tolerate the campus's safety hazards and getting paid in vouchers, quits when Dean Pelton enables Chang's insanity to avoid legal troubles, saying that it isn't funny.
Dexter: Complete Jerkass Quinn shows up at a party thrown by his ex-girlfriend, Debra Morgan, and he has some anonymous slutty girl with him. He acts drunk and stupid enough to annoy everyone at the party, but the slutty girl doesn't mind, until she finds out that she's only there to make Deborah jealous. "You brought me to your ex-girlfriend's house? Have a nice life."
On Friends, most of the gang avoid Janice like the plague for her Annoying Laugh and general annoyingness. Ross, though, dates her for an episode because he's in a down period in his life and feels he can vent to her. He takes it too far even for her, though.
Ross: You're saying that I've become so whiny that I annoy you. Janice. Janice: Well, yeah. Ross: Oh.My.God!
How I Met Your Mother: Barney, despite being obsessed with sex, considers it valid when one of Robin's boyfriends breaks up with her due to a bedroom thing that made him feel uncomfortable.
Impractical Jokers: The guys will say a lot of rude and insulting stuff, but every last one of them has a hard time talking that way to women. Especially pretty women.
JAG: When Admiral Chegwidden was President of the Captains Promotion Board, Commander Lindsey's name came up. When asked about his views on the man, Chegwidden stated he would not willingly have the man on his staff as he breaks too many rules. The person who asked this just stares for a moment as he personally has dealt with Harm and his unorthodox methods.
Revolution: In episode 11, Jason Neville decides that slaughtering groups of people with air strikes is completely unacceptable. His father does not react well to his son taking a stand.
In another episode, one of Alan's patients is paying him extra because she gets sexual pleasure from his chiropractic skills. When arguing with Charlie about it, Charlie retorts that he may have done some things he wasn't proud of for the sake of sex, but he has never taken money for it (that's not to say he hasn't paid someone else money for it).
Will and Grace: Lionel thinks Karen is a hooker when he first meets her:
Lionel: You don't have sex for money?
Karen: No, I do not! For jewels, for furs, for mixed securi*ties, like a lady! But for money? How dare you!
In The 100, even the strictly controlled dystopia of the Ark is reluctant to execute children, despite Population Control being a basic necessity. It'll lock them up until eighteen and then execute them if their trial doesn't go well, and it'll use them as lab rats to determine if Earth is habitable again, but they are at least given something of a chance.
In an episode of Nip/Tuck, the show's Casanova Christian Troy hooks up with an attractive mother and daughter pair in a night club. When they go at it again the next morning, the daughter reveals that they started doing this when her mother caught her making out with her stepdad, disturbing even Christian. He eventually concludes "This is too screwed up, even for me", and throws them out of his apartment.
In Tides Of War, there is a double example. Anduin, a pacifist, realizes that Garrosh has gone too far and must be stopped in the wake of Theramore's destruction, but is glad that his more warlike father Varian isn't going as far as Jaina originally planned to.
In Beyond the Dark Portal, Turalyon, who has no love for the orcs (the epiphany he had in the previous book that cleared all his doubts was the realization that the orcs are not from Azeroth and not part of the Holy Light), is quite disturbed to see Alleria's obsession with killing the orcs to avenge her dead brother and all the people she lost in their invasion of her homeworld, which has resulted in a rift growing between them, and which causes him and the rest of their friends to become worried about her getting herself killed in her quest for revenge. Turalyon's also quite disturbed when he sees an orphaned boy playing with a sword and wanting to grow up and kill orcs.
In World of Warcraft, during the quest chain that leads you to the Temple of the White Tiger, Horde players can ask Sunwalker Dezco, a tauren paladin, whether it would be a good idea to capture Anduin Wrynn, prince of Stormwind, right then and there, as he's negotiating with Xuen, one of the August Celestials, for the right for the Alliance, Horde and Pandarian refugees to enter the Vale of Eternal Blossoms. Dezco does not approve, saying this would make you no better than Garrosh, and that he has no desire to fight against an unarmed child, do so in the temple of an August Celestial or bring harm to someone who has earned his chieftain's respect.
Garrosh Hellscream himself has a considerable list of tactics he considers unacceptable; blowing up civilian settlements, recklessly launching a surprise attack on the Alliance and undermining the efforts against a mutual enemy, using fel magic, raising the dead, using the Plague of Undeath, and various others such as Mind Control. However, it seems he may be abandoning at least some of those standards considering that he has enslaved molten giants, blew up Theramore and plans to weaponize the power of the Sha.
In No More Heroes 2: Desperate Struggle, Henry offers help with a particularly troublesome multi-form boss. When he sees the (incredibly ridiculous) final form, he just up and leaves.
Henry: I can't be associated with that travesty. I've got standards, for fuck's sake.
In Katawa Shoujo, Kenji is a misogynistic conspiracy theorist who apparently cannot find anyone else to join his cause, but he won't accept people who like futanari porn. It's also indicated that he had a girlfriend once, possibly Yuuko, and that he doesn't have a problem with women in general, but feminists.
Hikawa's backstory in Shin Megami Tensei: Nocturne has him get rejected by The Gaea Cult because they considered his beliefs too extreme. To elaborate, this is a cult of demon worshippers we're talking about!
In Mass Effect, Ashley Williams is somewhat distrusting of aliens, inasmuch as she wants humanity to not have to rely on them. Even so, she doesn't support the Terra Firma party, and she's particularly strongly against Cerberus.
In Quentyn Quinn, Space Ranger, while it should be noted Dweebly is an Only Sane Man compared to the other Fedorks we've seen so far, he takes one look at the shuttle schematics presented to him here and burst out laughing, thinking that it's some kind of joke! Even his species, which flies around the galaxy in structurally flawed (they have something else holding them together) ships fueled by antimatter (which is obsolete in the titular character's civilization), wouldn't rate the shuttle safe for flying above 1,000 feet, let alone space (this is due to the fact that there are no backups or spares of any kind, something that every good engineer includes), even saying that the Fedorks would consider this design suicidally insane! Cue epic Oh Crap when he realizes that it's not a prank, and that the shuttle actually exists!
In Sonic The Comic Online even Sonic is pretty disgusted when he gets a chance to see just how much of an ass he was in the past.
90s Kid of Atop the Fourth Wall loves extreme things, but killing pregnant women is not extreme to him. Thus a scene in a comic where that happened got him depressed, and it took a Rob Liefeld comic to get him better. Much later, his fondness for "Extreme" names wavers when even he admits the name "Death Blow" is kinda lame.
SF Debris was noting a particularly sexist scene in a Star Trek episode, and said he was offended, even though he thinks porn should be on the food pyramid.
Roboshi, the infamous fav'ing machine of the Ponibooru Crufavers, has quite a images that he will never fav.
"No fav for you, goo man chu!"
One of the tentacle monsters in Ghastly's Ghastly Comic thinks it's wrong to sexually assault human women, although this is at least in part due to a personal distaste for them.
Hilariously lampshaded by Mexican mock-newspaper (like The Onion) El Deforma: When Enrique Peña Nieto was featured on the cover of Time Magazine with the caption "saving Mexico", Mexicans were outraged at what they perceived was a propaganda stunt. El Deforma then issued a note that said that Time Magazine had taken it "too far on their joke" and that even they wouldn't publish a news that would upset the majority of Mexicans.
Although the cul-de-sac kids despise The Eds (usually Eddy), they are frightened and appalled at the beating that Eddy received from his brother.
While Glenn Quagmire of Family Guy does a lot of perverted things, there are some lines he won't cross, like sleeping with Meg when she's desperate for affection (and underage). Of course, once she's 18, she's fair game. Quagmire also doesn't like when a woman is attacked, especially when he learns his sister, Brenda, was being abused. He goes has far as killing Jeffery Fecalman, Brenda's abusive boyfriend, for this.
Archer is the biggest man whore you will ever meet, but he refuses the 16-year-old Anke's advances, even though she's legal where they are (in Switzerland.)
Pinkie Pie in My Little Pony: Friendship Is Magic is a prankster and loves pulling one over on her friends. However, she absolutely will not prank the sensitive Shrinking Violet Fluttershy, acknowledging that even her softest prank could potentially hurt the shy pony's feelings.
Lots of people in The Simpsons have this attitude towards the titular family, either towards individual members or the whole group. A great example would be in "The Cartridge Family", where Homer joins the NRA, a group notoriously in favor of the right to own guns. After seeing him fire guns in an extremely reckless way and endangering everybody, they kick him out and try taking away his gun.
In a Valentine's Day anthology episode of The Simpsons, Marge and Homer play Bonnie and Clyde, while Flanders is their unwitting getaway driver. Upon seeing that they're outlaws, he is shocked, but cuts them some slack. It's the Depression, and people are desperate. However, once Flanders learns they are an unmarried couple, he decides to turn them in.
Codename: Kids Next Door: The KND aren't fans of adults and fight adult tyranny, but what the bullies do to them in "Operation: M.A.T.A.D.O.R." (trapping them, giving them Klatchian Coffee until they fly into a rage, and then fighting them in an arena) is a line they will not cross. Numbuh Four is scolded by his teammates for participating in this demented pastime. Specifically, they acknowledge that for how anti adult they are, the ones they go after are clearly insane super villains. They fully refuse to go after adults that they know are just innocent bystanders.
Captain Stickybeard hates the KND and has battled them more than once. However, he's willing to aid them sometimes because he thinks that eating vegetables is too cruel.
In Celebrity Deathmatch murder and torture are totally fine while in the ring (and sometimes even outside), but in Fandemonium II when Ozzy Osbourne enslaves Rob Zombie and makes him give him a pedicure, even Johnny and Nick think he's crossed the line. The referee, Mills Lane, has no such reservations, however.
Even David Van Driessen has limits if anyone touches any student, Beavis and Butthead included. This was shown in "Manners Suck" when Beavis calls Mr. Manners/Candy a pervert upon being attacked, causing Van Driessen to take actions that goes against his 'hippie' ways.
Out of all their actions, Beavis and Butthead themselves have limits. Butthead can’t stand seeing another guy’s privates, especially Beavis. This was demonstrated in "Tainted Meat" when Beavis has a problem with his privates and being told by Butthead not to show him… to the point Butthead gives suggestion like telling him wash it which, rarely happens, cured Beavis's problem. Beavis often has to deal with Butthead’s taunts, but he has his limits. When Butthead pushes to far, like calling him Butt knocker one too many times, Beavis beats him up, such as kicking him in his privates as seen while watching some music video.