You're hearing it in your head right now, aren't you?
Kent Brockman: Alright, let's go live to Bob headquarters now, for Mayor Terwilliger's victory speech. Sideshow Bob:(approaches podium)Ahem. Heh, heh, heh. Hah, hah hah, hah hah! HA HA HA HA HA!! Kent Brockman: And just look how happy he is!
In any case, it has since devolved to the occasional evil chuckle (which may or may not be a bit scarier) or so every once in a while, except in certain cases, and the trope of laughing while your victim is helpless is definitely dead, as modern villains usually laugh while they work (to much better effect). A common subversion is to have a villain have an Un Evil Laugh, with snorting, chuckling, and squeaking especially if they are a villain in a comedy series.
In anime, haughty female villains (as well as some male ones) will overlap this with Noblewoman's Laugh. Other villains, for reasons known only to themselves, go for the Giggling Villain approach. Often goes well with Drunk on the Dark Side.
It's even more jarring (and awesome) when the villain in question is an emotionlesscharacter.
Just remember, you Genre Savvy villains, the Evil Overlord List:
20. Despite its proven stress-relieving effect, I will not indulge in maniacal laughter. When so occupied, it's too easy to miss unexpected developments that a more attentive individual could adjust to accordingly.
Chapter 458 gives us an awesome one, so much the two speech balloons that contain it occupy together a good half of the page. Who provides it, you ask? Ginjo, and this time, it isn't an act.
During his fight with Rukia Kuchiki, Espada #9 Aaroniero Arruruerie repeatedly uses a vicious laugh.
Kageroza Inaba in the anime during the Gotei 13 Invading Army arc, particularly at the end of episode 333 and the beginning of episode 334.
Torpedo Girl from Bobobo-bo Bo-bobo laughs using the word "Torpedo" over and over again. It's really creepy.
For the protagonist of the series, Lelouch from Code Geass gets an inordinate number of evil laughs, usually when he's just pulled another utter pwnage on his enemies with some unexpected genius tactic. His most maniacal ones were in episodes 17 and 23 (quoted below) of season 1, and episodes 2, 7, and 9 of R2.
Lelouch: These hands of mine have been dirty for a long time now, Suzaku. Your coming to face me now doesn't matter at all. Hell, I welcome it even. I mean of course, you and I are friends.(starts laughing maniacally as the Tokyo Settlement collapses around him). 
His first is a sad laughter, too, after he learns that his best friend Suzaku is working for the Evil Empire that he hates to the bones.
The most maniacal ones are notable in that they start out as a low chuckle, then rise in pitch to truly deranged sounding levels.
Emperor Charles' dub laughter somehow manages to exceed Norio Wakamoto's laughter. Michael McConnohie takes it to more than just 11, he takes it to 13. AHAHAHAHAHAHA!AHAHAHAHAHA! Check it out at 8:18-8:22.
Light of Death Note indulges in an Evil Laugh when at his most sinister. In fact, in the final story, he struggles to hold in his imminent victorious laughter and ultimately fails in a scene that cranks the full-blown psycho version of the evil laugh Up to Eleven. Sanity's gone at this point. He cracks one during the infamous potato chip scene.
Lampshaded in an episode of Digimon Adventure 02 (dub only) when the Digimon Emperor burst into a fit of evil laughter after expounding on one of his evil plans, then stopping and muttering "Eh, it's not that funny..."
Myotismon has a pretty good evil laugh too, particularly in the first season. Used so much in the dub that the other characters comment that they're "sick of that laugh". His laugh is so awesome that the dub's editors rewound a clip of him giving a skyward cackle twice.
In Digimon Xros Wars, it's particularly hard not to find DarkKnightmon laughing whenever he talks.
Katsuhiko Jinnai from El-Hazard: The Magnificent World (particularly the OVAs). In the original Japanese, he simply sounds like a megalomaniacal Evil Overlord [tm] when he laughs. In the dub, he sounds completely unhinged. It's both hysterically funny and extremely creepy at the same time.
Hokuto No Ken is full of this; almost every major villain does this.
Even though he's an Anti-Villain, Greed of Fullmetal Alchemist has a distinctive evil laugh/chuckle, which is generally translated as something like "gwa-ha-ha-ha-ha". On a couple of occasions in which he's Laughing Mad, it can be kind of disturbing. The psychopathic Envy provides a straighter Evil Laugh on ocassion. Also, while generally a collected Affably Evil, early on, when he sees explosions and chaos going on, Mad Bomber Kimblee starts laughing insanely. Finally, toward the end of the series, Father does a really disturbing Evil Laugh while carrying out his master plan, which is particularly scary because he has Voice of the Legion at the time.
Frieza, of course. Though, he often laughs like a lady.
Full Metal Panic!!: A teenage terrorist in his enormous Humongous Mecha unleashes a hysterical yet pretty normal laughter while devastating a city. However, megaphones in his mecha repeats his laugh all over the city, so it sounded sufficiently evil.
Fushigi Yuugi'sTomo, surprisingly, does this more often than Nakago, who can't even match up to the former's signature laugh ("Kakakakakakaka!"). Mayo Sakaki, for a bratty 16-year-old, has an evil laugh creepier than Nakago's.
Penchinon from GaoGaiGar has his "BREEEEEEEEEEEE!!!"
Heero Yuy from Mobile Suit Gundam Wing does this in the first episode but drops it after that. Chief Engineer Tubarov does it too, just before his death.
Char Aznable does this every once in a while. In the original series, he does this when setting up the misfortunate death of his own best friend, Garma Zabi, for revenge for his father. In Char's Counterattack, he does a grand one after losing to Amuro Ray in their final duel but successfully dropping a half of Axis onto Earth.
Many, many protagonists gone Ax-Crazy in Higurashi no Naku Koro ni, including the one pictured above. Yes, it's creepy. Shion hands down, though, seems to erupt into evil laughter a lot, specifically the 5-8 episode arc. In fact, someone even made a ten-minute long loop of her laughing when Rika dies in a later arc. Have fun sleeping tonight, by the way.
Rena is prominent for crazy laughing, too.
Yuno Gasai from Mirai Nikki erupts into this big timeafter she manipulates Yuki into killing Hinata. Just another reason why she's the prime example of a Yandere.
Katekyo Hitman Reborn! features two characters that fit the trope: Belphegor's "Ushishishi!" and Mukuro's "Ku fu fu~" — the latter character actually managed to score an Image Song titled "Kufufu no Fu". Both character's image songs feature solos of them doing their laughs.
Xanxus also had one, although it's less unique and well known compared to the other two previously mentioned. He tends to have it whenever he goes off ranting about how everyone else is trash.
Even Byakuran got one during his fight against Tsuna. He sounds like a clown.
Keroro himself likes to think he has one, but it's just "devious" (KEEEEro-kero-kero-kero).
Supervillains (Heel wrestlers) in Kinnikuman tend to have very distinctive laughs. There are many examples, but some of the best examples are those of Ditto Fighter Stecase King ("Kekekekekeke!") and Joke Character villain Kinkotsuman ("Muhyohyohyooo!")
Ashuraman's laugh ("Kakakakakaka!") is so distinctive, it's actually used as a lyric in his theme song.
Friday Monday from Madlax is of the third category, perhaps even the epitome of the third category, as he sometimes struggles to contain his evil laughter in times of victory and will cackle for quite some time.
Despite being called the "The Uchihahaha", Itachi's Motive Rant did not actually involve laughing (though it sort of looked like he did), just overacting. An event which truly lives up to that name comes nearly a hundred chapters later, but uttered by Sasuke. Not simply because he was feeling evil, but to mock Kakashi when he tells Sasuke to stop being so obsessed with revenge.
One Piece not only has several characters performing the Evil Laugh, but usually, every character who performs the laugh has a unique and distinct version of it that only that character says. For example, Marshall D. Teach aka Blackbeard's signature laugh has a "Ze" sound to it, and Sir Crocodile's laugh is dry and monotone.
Rosario + Vampire: Inner Moka, while not evil, has a couple instances of this in Season II: in chapter 1, after dishing Tsukune punishment for touching her and telling him he has a slim chance of seducing her ("Want to know your odds?"), and again at the end of chapter 6, right before curb-stomping the doppelganger that dared mimic her body and abilities.
Rozen Maiden: While definitely far more mischievous than evil, Suiseiseki manages to pull this one off pretty well when playing pranks on the other characters, and once when playing the evil queen from Snow White in a rehearsal for a school play, she does it in an extremely convincing manner.
For a good guy (he's even The Messiah), Touma manages to pull off an evil laugh. This is possibly the first time in the history of fiction that this trope has been weaponized. The guy running away in terror? He's a freaking Reality Warper.
Accelerator gets these pretty often. In the anime they sound freakishly high pitched and mixed in with a kind of coughing noise, to further illustrate how unhinged he is. Then there's that... sound he makes when fighting Touma: KHKHKHKHKHKHKHKHKUKUKUKHIKAKAKAKAKAKAKA!!!!
AKIRA: Tetsuo lets out some memorable maniacal laughs.
Niwe, The Caligula from Utawarerumono, is known by fans for his intensely annoying "GYAH KA KA KA KA KAT" laugh. He would occasionally use it more than once per episode. Apparently, he was known for it by characters too, since just when Hakuoro thought they were rid of Niwe, his laugh returns with a vengeance in his dreams.
The Vampire Princess MiyuOAV. Miyu is sometimes heard laughing in the end of each OAV and it's a soft, child-like, almost gentle laugh. However, due to the circumstances and Miyu's own nature, such giggles potentially can be more horrifying than a typical Evil Laugh.
Most villains in Yu-Gi-Oh! tend to have an evil laugh, but Yami Bakura's stands out the most. He starts constantly laughing at the start of the Battle City Finals, and those laughs get more nightmarish in the Millennium World Arc. Bonus points for being voiced by Rica Matsumoto.
Nearly all of the villains in the dub. Also, in the dub, almost every episode ends with an evil laugh, and some begin with them, too.
Professor Cobra in Yu-Gi-Oh! GX, as well as the appropriately named Brron, Mad King of Dark World.
Saiou/The Light, has the creepy high pitched kind that illustrates his insanity excellently.
Yusuke has some fun with this one in YuYu Hakusho. After dying a second time and reincarnating as a half-demon, he does this along with a little speech to toy with the elite squad of soldiers dispatched to destroy his corpse and prevent said reincarnation. They fall for it, if only because it's what they expected to happen anyway, and he has a good normal laugh at their expense immediately afterward.
Elder Toguro also has a creepier version of this trope played straight.
Prince Sharkin in Raideen had an evil laugh that was extraordinary in its legnth, volume, resonance, and the sheer amount of scorn it conveyed. It was so epic that his voice actor got typecast as villains for the next decade.
In Princess Tutu, Drosselmeyer is very fond of his evil laugh. He's an old, insane character, which makes him a pretty good example of the evil wizard type. Mytho also starts letting out evil cackles in the second season after their Face-Heel Turn.
Even though Aion from Chrono Crusade is often smooth and charismatic, in his more manic moments, he can let out an evil, crazed laugh of epic proportions.
Also parodied in one of the specials. Yukimura gets the idea that his team has to have more fun while playing. Sanada and the aforementioned Kirihara promptly try to please, revealing that those two just CAN'Tlaugh normally. Poor Yukimura has no choice but to give up.
He laughed the bony child to scorne That was the bonny Lord of Learne. He laughed that bonny boy to scorne; Lord! pitty it was to heare; I haue herd them say, and soe haue you too, That a man may buy gold to deere.
Batman's enemy, The Joker, is the archetypical example of the first type of exception (The Joker is also a bona-fide psycho, prone to impressive cackling). In fact, in the "Die Laughing" crossover with Judge Dredd, when The Joker manages to join the Dark Judges, his power is a laugh so mighty that it kills people.
See also the various Batman adaptations, below.
Appearances of superhero The Creeper in The DCU are accompanied by a high-pitched, manic 'Yamazaki is playing Stabby Needles' laugh with an unhealthy amount of girlish glee. Evil giggle works. In the later versions, his laughter actually caused damage.
Lampshaded in one Spider-Man comic when the Shocker tries it, only to be shot with a tranquilizer dart by the mercenary villain the Pro, who states that he hates evil laughs, calling them "unprofessional".
Storm is one of the good guys, but in one issue of Marvel Adventures, she gets to play the part of a weather witch. Maniacal laughter is part of the package.
Lina Eastwood/Cure Western from Pretty Cure Hollywood Stars is a rare heroic example of this trope, and BOY does she make the most of it! She tends to laugh evilly in her hammier moments, such as in episode 12.
In The Hunt for Harry Potter a non-villainous friend of Harry's called Henchgirl said that because she was a mad scientist, "the rules" stated that she had to cackle at least three times a day. When he later found himself cackling madly after completing some correspondence tests that he felt certain he'd pass, he muttered that "Maybe it's time for me to expand my circle of friends to include people that don't think the laws of physics are . . . how did she put it, 'silly little suggestions?'"
When Chrysalis is brought into the Ponies Of Olympus series, literally the first thing we see her doing is coaching her daughter Aurelia in how to do one of these properly.
Hago, Shocker, Death, and many other villains from The Lion King Adventures are quite proficient at the art of evil laughter.
Films — Animated
Villain Songs have a tendency to end with the villain laughing maniacally.
Few villains rock the Evil Laugh harder than Evil Chancellor Jafar on Aladdin, especially at the end of his song. In the Crossover with Hercules, Hades calls him on it. When Jafar actually convinces Hades to give it a shot, the big blue guy admits that "It's cleansing."
In the final battle scene of Beauty and the Beast, Gaston does an evil laugh before he taunts Beast.
A prize example (not from a villain, but still quite scary) is given by the castle's stove.
In Flushed Away, the Toad and his henchrats (including Spike) do evil laughs repeatedly.
Hexxus from Ferngully: The Last Rainforest has a laugh at the end of his extended song that ends with a dry cough. Can be seen as a satire, but actually justified, as Hexxus is the embodiment of pollution.
He indulges in some much more frightening laughter when he assumes his One-Winged Angel form.
Ratigan from The Great Mouse Detective, after his attack on Basil. Voiced by Vincent Price. Can't get much greater than that.
Ursula from The Little Mermaid lets out an evil laugh so friggin' scary while Ariel turns into a human that it shames the Wicked Witch, Jafar, and Maleficent put together.
She's also got this smug snarl/chuckle thing going on in other scenes, too. It's wonderfully malicious.
In Hoodwinked Too! Hood VS. Evil, Moss does an evil laugh at the bridge scene after an 18-year-old Red tells him, "Bring it!".
In The Lion King, a young Simba laughs evilly after he says, "I laugh in the face of danger!" in the Elephant Graveyard. Also, Scar lets out a particularly great evil laugh at the end of his Villain Song "Be Prepared".
Well, when your henchmen of choice are hyenas, you've got a lot to live up to.
Surprisingly, Ed, the Cloud Cuckoo Lander of the Terrible Trio who spent the movie laughing like a moron, actually had one of these at the end of the movie, shortly after Scar denounced the hyenas to Simba. When Shenzi and Banzai ask Ed if they heard Scar correctly, Ed lets loose a disturbingly low chuckle punctuated by him licking his lips hungrily, looking intense for the only time in the whole movie. Then, the hyenas close in on Scar, all giggling murderously, and his desperate begging (and later screams) is eventually drowned out by the sound of their laughter.
In the sequel, Zira has several. One was heard towards the end of her Villain Song, and another is when her lioness army leaves Simba to be attacked by her during the final battle.
The Coachman from Pinocchio has a rather creepy evil laugh after he tells Foulfellow and Gideon "They'll never come back...as BOYS!!!" And yes, both Foulfellow and Gideon were both extremely frightened by the Coachman as soon as they heard him say "BOYS!!!"
In Dirty Harry, the Scorpio Killer has a sort of gibbering, intensely self-satisfied laugh that is in no way appealing. His final moments are even characterized by one last split-second of this laugh when he decides that yes, he does feel lucky.
Subverted in Inglourious Basterds: Shoshanna does this after delivering her message to the Germans (that they are all going to die). It fits the trope in every respect except for the part that Shoshanna is a hero.
When Jim Carrey plays a villain, he sometimes does a laugh like this, most notably the Grinch and his performance as Olaf in Lemony Snicket's A Series of Unfortunate Events. On the Interactive Olaf DVD feature, he does do an Evil Laugh in one part.
As Olaf, he actually goes through several different Evil Laughs at one point, trying to find his favourite.
Also, he has a pretty sadistic laugh as Lloyd in Dumb and Dumber after he tricks Harry into drinking hot chocolate with a laxative - complete with Wicked Witch of the West theme.
Gets one in Batman Forever watching Two-Face holding the circus hostage.
In Jet Li's Last Hero In China (one of many, many names for this movie), the main villain has a ridiculously good evil laugh. Actually, it's more like an utterly psychotic laugh, at entirely wrong moments. "Wong Fei Hong! Help me! He has broken loose! BWA-HA-HA-HA-HA-HA!" "Our evil master will not be pleased! BWA-HA-HA-HA-HA-HA-HA!"
In the movie finale, he got his knee broken (after trying to attack Jet Li's character from behind). Then Jet Li's character says: "Now you can laugh." He sobs like a little child.
Murder by Death. At the end of the movie The maid enjoys a weird evil laugh after fooling all of the detectives.
The Predator managed a rather decent version when he was all set to blow Arnie and half the countryside to oblivion. (It was a recording of Billy's laughter from earlier in the movie, albeit in a creepy, distorted manner.)
This is almost an inversion, since it's not really an evil laugh; it's a normal laugh that just sounds insanely evil because of the context and the way that it becomes distorted.
Raul Julia, in every villainous incarnation. His subtler chuckles only need the broad, manic grin to crank up the menace (or hamminess).
Jimmy, the #2 thug from Road House, lets out a sinister cackle as he rides away after blowing kindly ol' Emmett's house to smithereens (luckily, Emmett is rescued). Mike Nelson is quick to hang a lampshade this in the Rifftrax commentary:
Mike: It only now struck him as funny; now that he's got some distance behind him.
Emperor Palpatine of Star Wars is of the second sort.
Jabba the Hutt is a good example. Ho ho ho ho ho ho... (Han Solo doesn't need his eyesight to recognize Jabba. "I know that laugh..." says Han.)
The Joker in The Dark Knight seems to be mocking the trope the first time we hear him doing it (when he walks into the meeting of the gang-leaders). Later, he indulges in plenty of straight uses, up to the point of doing it while falling off a skyscraper.
He's certainly mocking it when he gets a kidnapped newsreader to read his threat to Gotham City from cue cards, including the words "Ah-ha-ha-ha".
Jack Nicholson's impressive cackling as the Joker from the 1989 Batman film. Nicholson's Joker keeps on laughing even after he's dead. Sure, it was prerecorded and looped, but still.
Scaramanga from The Man with the Golden Gun has some recorded ones set up in his funhouse for when someone shoots at a picture of him. Oddly, Scaramanga is played by Christopher Lee who, while known as the quintessential villain actor, doesn't usually have an evil laugh.
In The Matrix Revolutions, after Agent Smith absorbs the Oracle, he lets out an eerie, utterly out of nowhere evil laugh made even more creepy by the fact that Smith is usually stoic. (here)
In The Wizard of Oz, the Wicked Witch of the West has a very frightening cackle.
Oz: The Great and Powerful has Oz himself do this after faking his death and revealing himself in the form of a gigantic, ghostly head. The Witch of the West retains her own cackle in this film, but in Oz's case, he's simply returning the favor.
Pick a character, any character, played by Vincent Price. Even when the Phantom Manor at Disneyland Paris re-recorded a good chunk of his narration, they left in his laugh because it was that distinctive.
John Jarret worked on the evil laugh for six months when he was cast in Wolf Creek.
In Tower Of Terror, Emmiline gives out one in Abigail's flashback. It turns out to be a lie, though.
You cannot actually hear Lon Chaney laugh in the 1925 version, but he looks utterly terrifying as he does it, making this the scariest laugh you've never heard.
The Phantom laughs evilly to mock the policeman who tries to shoot him in the 1989 version.
King Ghidorah, Godzilla's Archenemy, has a high-pitched, Joker-esque cackle as his trademark. The laugh actually serves a purpose in his case, as it helps to demonstrate that, unlike most of the other monsters in the series, Ghidorah is fully aware of what he's doing and thoroughly enjoys it.
Parodied in The Master of Disguise. A Running Gag was that every time Brent Spiner's villain character started cackling, he would fart. (They actually switched it out at one point, allowing him to finish the laugh, then fart.) Truly, humour for the ages (specifically, the ages between six and nine).
Also, in the scene where Ralphie gets a C+ on his essay, he imagines Ms. Shields (dressed as the Wicked Witch of the West) and his mom (dressed as a jester) singing "You'll shoot your eye out!" and then laughing evilly.
Tex Richman from The Muppets is unable to laugh, so he says "Maniacal laugh!" instead. When Uncle Deadly does his Heel-Face Turn, he rubs his own glorious maniacal laugh in his face right after himself delivering the most spectacular Evil Laugh of the entire film.
In the film Hook, the titular character gets a particularly nice one that's sort of segmented: "Wah ha, wah ha, wah ha ha ha ha ha ha". He does this after coming to a decision, and it works beautifully.
In Evil Dead 2, a whole room, starting with a stuffed deer, mocks Ash. He ends up pulling out his own crazy laugh. See here.
Adele Hasn't Had Her Dinner Yet: Baron Rupert von Kratzmar (Miloš Kopecký) is a Mad Scientist archetype and he has one of the greatest examples of this trope in Czech movie history, and perhaps one of the greatest Evil Laughs ever. It's really devilish. When a policeman on the street hears it, he just crosses himself and quickly runs away.
At the point Lost Highway begins to go insane, a mysterious man tells the main character to call his house. When he does, that same man answers him. And then both of the mysterious man burst into laughter. Be ready for it.
In Airplane!, airport employee Johnny offers up one of these after his For the Evulz prank involving the runway lights.
In Little Shop of Horrors (the 1986 musical version), both Audrey II and Orin Scrivener have some spectacular evil laughs, though Orin only does so because he's under the affects of laughing gas.
In The Great Race, Professor Fate and the Crown Prince of Carpania have two different evil laughs, despite both being played by the same actor.
In Warlock, the Warlock gives an evil laugh when a young boy asks him what he needs to be able to fly, which is to sacrifice the child.
Used for Troubling Unchildlike Behavior in Lolita (1997). Humbert knows that Dolores is in league with the Mysterious Watcher who's been following their car. At one point he gets angry and throws Dolores onto a hotel bed, but having known for some time how to manipulate him sexually, Dolores starts moaning as if they're having sex. As they start having sex for real, Humbert breaks down and pleads for her to tell him the truth, but Dolores just laughs.
In Scanners II: The New Order, The Dragon Peter Drak is prone to doing this after causing destruction and killing people, like at the arcade hall and after blowing up Gelson's head.
Count Olaf in A Series of Unfortunate Events has a distinctively wheezy voice and a laugh to go with it. In The Grim Grotto, he's doing his darndest to make it the most elaborate, obnoxious evil laugh ever. (This is great fun in the audio books with Tim Curry doing the reading.)
In later books in the series, Olaf deliberately inverts this by shortening his laugh to a simple "Ha!"
In the movie, Olaf kind of experiments with it near the end. On the DVD feature Interact Olaf, he demonstrates an evil laugh at one point.
There are many villains with Evil Laughs in Discworld. One of them (in Maskerade) writes his evil laugh down. With five exclamation marks. Sure sign of insanity.
A relatively minor character in Making Money has one of the best evil laughs seen in the series. His co-worker Igor laments that it is so unfortunate that he is neither technically insane nor evil.
Granny Weatherwax does not do this, but her genteel chuckle when she is about to do the Right Thing has been described as far, far scarier.
Witches of the Discworld in general try to watch themselves and each other for excessive cackling, which is a sign of going mad and turning into a fairytale-style wicked witch.
Lampshaded with Lord Hong, who would only have laughed maniacally if he were the traditional type of Evil Grand Vizier.
Lampshaded by Harry Dresden in Grave Peril. At Bianca's party, the lights go dark, and spooky laughter comes out of nowhere. Harry notes that it's tough to beat vampires at the creepy laugh. They get a lot of practice.
Harry points out an Evil Laugh again in Dead Beat when he encounters the vampire Mavra. He also gives a fairly apt description of what makes an evil laugh.
Maybe it was the atmosphere, but something about it, the way that it simply lacked anything to do with the things that should motivate laughter... There was no warmth in it, no humanity, no kindness, no joy. It was like Mavra herself — it had the withered human shell, but underneath it all was something from a nightmare.
In Edgar Rice Burroughs's The Gods of Mars, when John Carter and Tars Tarkus enter a chamber, the door closed behind them and "And then, from unseen lips, a cruel and mocking peal of laughter rang through the desolate place."
In the Harry Potter series, Lord Voldemort and Bellatrix Lestrange. Subverted with Sirius Black, who had one, but turned out to be a good guy. Voldemort's "high, cold, cruel laugh" is one of Harry's earliest memories (immediately after the flash of green light that killed Lily).
Quirrell does one after being revealed as a villain at the end of the first book.
Doctor Impossible in Soon I Will Be Invincible declares in the narration that he will have the last laugh, "and I have a very good laugh." Damsel says to the other heroes "He's out there somewhere, probably half a kilometer underground. Laughing his freaky laugh. Talking to his robots." When he captures them, he lets it rip.
Saint Dane, of The Pendragon Adventure fame, has a trademark laugh that Bobby hates having to listen to — probably in part because half the time, it means he's about to hear how he's been outsmarted this week.
While it is normally left to the imagination, there is a spectacularly chilling example from the audio-book of The Rivers of Zadaa. Who knew William Dufris had it in him?
In "The Scarlet Citadel", Pelias' laughter at a dead man is not pleasant. Even his comment that the dead man tortured young men to death doesn't keep Pelias from creepiness — especially since he then proceeds to raise the dead man as a zombie to open the door that he and Conan need to get out. Even when they're on your side, sorcerers in the Conan universe are creepy.
“a hearty and insane laugh, about as far removed from polite after dinner laughter as you can get. That was the kind of insane laughter even the truly insane have to practice for years to get right.”
In Galaxy of Fear: Spore, Tash's little brother gives one because The Virus is using his body to tell Tash to give up and be assimilated. She's surprised at how sinister he sounds. Lampshaded later.
"So I have an evil laugh, huh?" Zak asked. He gave a wicked-sounding chuckle.
Live Action TV
Smallville: In one episode, Lana, Chloe and Lois get particularly chilling ones due to being possessed by evil witches.
Buffy the Vampire Slayer: The nerd trio in season 6 : "We're like... supervillains. Mwahahahaha!" (The script notes "It needs some work"). The most complete counter-example is the Buffy season 3 ubervillain, the Mayor. His chuckles and general good humour are completely sincere and natural.
Angel: Lampshaded in one episode when Lorne describes a scene where the Big Bad "mwa-ha-ha'd at us." Played extremely straight (and sinister) when the episode "Awakening" ends with Angelus opening his eyes and...smiling. Then chuckling softly but continuously until the credits.
Ashes to Ashes: Jim Keats has a seriously creepy giggly one. And in the finale, when he's implied to be none other than Satan himself, he combines it with a weird hissing noise that's pure Narm Charm.
Batman: Frank Gorshin's evil, high-pitched chuckle as The Riddler in the Adam West series.
This cop/writer TV show had an awesome evil laugh moment in the Halloween episode. The main character, Castle, has an impressive go at it with a running pumpkin drill in his hand and a mild threat to go after any boys who hurt his daughter. Impressive, that is, until he has a coughing fit.
He has a more successful one when he suggests building a weather machine out of liquid nitrogen so that Alexis won't have to choose between staying at home to study and going with her friends out of the city.
Cheers: In the episode "The Executive's Executioner", Norm gets saddled with the soul-crushing job of "office hatchetman"; in the end, he uses his new boogeyman-status to get revenge on the executive who inflicted it on him: "This is NORM PETERSON! MUHAHAHAHA!"
Chuck: Parodied in "Chuck Versus The Subway" by Daniel Shaw, after he has captured Sarah and Casey. And then, in Chuck Versus the Ring, by Chuck, after he reveals to Daniel Shaw how he beat him and took down the Ring.
Some, no, any incarnations of the Master worth their salt have a penchant for dramatic mad laughter.
Jonathan Pryce's Master from The Curse of Fatal Death deserves a special mention. His Laugh is so Evil that it causes thunder and lightning to strike inside his TARDIS.
Davros combines this with a touch of Robo Speak at high volume for a memorably batshit insane Evil Laugh. If you're really lucky, it's probably the finishing touch on a spectacular Motive Rant.
The Black Guardian's version is good, if rather traditional; it goes something like "nyaaaaahahaha". Captain Wrack, meanwhile, seemed unable to function if she didn't cackle evilly every two minutes or so. The episode Enlightenment, where they both appear, makes for some interesting watching.
The Rani plays this trope straight in Time and the Rani, but in the mine shaft scene with the Master and Peri in Mark of the Rani, she starts choking and knocks Peri out with her poison gas capsules.
The End of Time has John Simm's Master, having just turned the entire of humanity into copies of himself, go into an Evil Laugh that lasts 45 seconds, is echoed by all his copies, and gives us the sight of John Simm in drag.
"Flesh and Stone" has the Angels making a very alien sound that is an approximation of laughter.
Joey's stalker (played by Brooke Shields) has a pretty scary laugh.
Good Eats: Alton Brown of does it occasionally, usually when turning a food trope into a Subverted Trope. Most recently used when getting his veggiephobic niece to eat vegetables (specifically the parsnip).
When Tommy was evil in the first season, he tended to do this quite a bit during that particular arc, and it got hilarious very quickly.
"I'll be back! You haven't seen the last of Rita Repuls-ah-AH-HA-HA-HA-HA-HA!" Hear it yourself here, starting at 1:36.
Almost all of Lokar's lines are just evil laughs.
Monty Python's Flying Circus: John Cleese does a surprisingly evil cackle of the psycho variety at the end of the sketch "How Not to Be Seen" (immediately following the explosion of a man, his wife, his neighbour, his house, and the building in which he was born).
Jerry does an epic one of these while smoking assholish cigars in one episode when Elaine is paying him money, having repeatedly lost her bet with him about not sleeping with David Puddy.
Parodied in the episode, "The Susie." Jerry suggests that Elaine should "get rid of Susie," an Invented Individual at her office. He then bursts into laughter at the sight of an offscreen bumper sticker. Unbeknownst to both of them, Mike, who's trapped in the trunk of Jerry's car, thinks they're planning to commit murder, and from his point of view, it sounds like Jerry is laughing evilly.
The blooper reel has several outtakes of Jerry doing his evil laughing while Julia Louis-Dreyfus keeps cracking up at him and ruining the take. Jerry eventually starts choking on all the cigar smoke on set in the middle of one of his evil laugh takes. It's hilarious.
The Mighty Boosh: One of the most underated antagonists in history, The Hitcher, provides an interesting subversion. Whereas most villains contain their evil laughter until the final moment, releasing it in one guffaw, The Hitcher laughs evilly AS HE TALKS, spreading it out through his dialogue!!
Senor Chang gets a couple of good ones in the episode "Modern Warfare". He also shares a less cackling one with Dean Pelton at an earlier point.
One of the German students does an evil laugh when he figures out a plan to get revenge on Jeff and the study group. He's in class when he does it, however, and tries to cover it up by claiming to remember a joke from the German version of The Nanny.
Blackadder: In the episode "Potato", we see, in turn, Captain Rum, Blackadder himself, and Baldrick try out what are definitely evil cackles of the pirate variety. They then stop as the line gets to a disconsolate Percy, asking him if he is not going to join in the "Ha Has".
Ultraman: Baltan's trademark laugh is widely known in the series.
Max: Whoever captured us has a terrific sense of humor.
Dollhouse: When Echo is in the Attic, she comes across a projection of Boyd Langton. When she asks where her friends are, he lets out a reverbating evil laugh before telling her she has no friends (he's lying of course).
The last track on their album Damnation and a Day features an evil laugh at the end.
"And the Earth...was without form...and void. And Darkness...was upon the face of the deep. Mmm hmm hmm heh heh ha ha. AH HA HA HA HA HAA!"
David Draiman's laugh at the beginning and end of "Inside the Fire". He does this perfectly live. He also does one in the beginning of "Asylum". From the same album comes "The Infection", which has a more amused, mocking laughter laying parts of the track ("And a thousand voices share their laughter at my demise").
Iron Maiden's "Fear of the Dark" usually gets this in live shows.
And it has absolutely nothing on the laugh at the end of "Moonchild". Now that's textbook evil laughter.
At the end of Metallica's Master of Puppets, a fairly sinister laugh could be heard.
Vincent Price ends his section of "Thriller" with a wry evil laugh, arguably doing more to relieve the tension than to amplify it. Man was creepy.
"These Hands" by The Damned tells the story of a demented circus clown, ending with the clown throttling someone and a rather fitting evil laugh. And then a slightly irritated "STOP LAUGHING!"
On Sesame Street, The Count would famously laugh in a somewhat sinister fashion after counting things out, accompanied by thunder and lightning. "I will now count these letters to myself! One! Two! Three! Four! Five! Five letters! Ah ha ha!"
It was originally a lot more maniacal, but was toned down to avoid frightening children away from learning to count.
Adventures in Odyssey: Dr. Regis Blackgaard has a memorable evil laugh that was quite effective in small doses. When he appears in the "Darkness Before Dawn" arc, you can hear bits of it trying to escape at certain points, but he holds it in until he finally takes over Whit's End.
A minute before the scene in question, he and his underling, Mr. Glossman, actually share a laugh together. The contrast makes him sound positively demented.
In a later episode, we learn that while his twin brother Edwin may be merely a hammy actor with an inflated ego, he has the laugh in his repertoire as well.
The Shadow is another example of a hero using the evil laugh — in his case, as psychological warfare. After all, maniac laughter from nowhere is unnerving, and criminals are a superstitious and cowardly lot...
The French summer radio show Les Persifleurs du Mal (Deadpan Snarkers from Hell) uses the Evil Laugh in its opening credits. It fits the show's general tone and what they do to some of the people they invite...
During Ross Noble's Randomist show an audience member admits he's looking at his watch because "he has to get up in the morning" and a lone woman laughs in a way that startles both Ross and the rest of the audience.
Ross: "Don't know why she's laughing like a mad woman. [as the woman] [maniac laughter] He won't be waking up to-morrow."
Dungeons & Dragons has "Tasha's Uncontrollable Hideous Laughter", a spell that can give any character the opportunity to let one of these fly. Also, a monster called the Gibbering Mouther has the evil laugh threaded through the cacophony of its constant utterances.
In the Ravenloft campaign, Esan the Mad, the Darklord of Vechor, not only has an Evil Laugh, but when he desires to seal the borders of his domain (a power that most darklords have) anyone who tries to leave Vechor against his wishes is struck by hideous, demonic laughter that only the victim that hear, which eventually drives the victim insane (in game terms, it causes Wisdom damage) unless he stops trying to leave Vechor.
Pathfinder has the Witch class, whose trademark ability is to use hexes; one of them is called Cackle, and it extends the duration of any other hex upon those within thirty feet by one round.
Chaos in Warhammer 40,000 specializes in this; most Traitor Marines having been turned batshit insane over ten thousand years of war and slaughter and the mind-warping effects of the Immaterium. Dawn of War has Chaos Marines randomly break down and cackle occasionally.
Damn Yankees has Applegate sing the Villain Song, "The Good Old Days", which, while sounding a lot like an inspirational song in the way it is sung, has Applegate laughing evilly in between stanzas.
The title character in Mozart and Da Ponte's Don Giovanni has a very unsettling laugh in the beginning of act two.
Older Than Radio: The aria "Vous qui faites l'endormie", from Faust by Charles Gounod, has a number of evil laughs written into the score. This is perhaps unsurprising, as it's sung by Mephistopheles.
The title role in Gilbert&Sullivan's "The Mikado" has some improvised Evil Laughs in between verses in his song about the CoolAndUnusualPunishments he's concocted.
In Act One of the The Phantom of the Opera, the Phantom breaks out in evil laughter when he ruins Carlotta's performance and later crashes the chandelier. Michael Crawford really makes the most of both occasions on the original cast album.
Though the Wicked Witch of the West is portrayed sympathetically in Wicked (and given the name Elphaba), she still gets her trademark cackle when Glinda slaps her.
Alberich in Richard Wagner's Ring cycle lets loose with an Evil Laugh occasionally, particularly in Siegfried, when Mime gets killed. Gustav Neidlinger pulled off a memorable one at the end of scene one of Das Rheingold on the classic Decca recording with Sir Georg Solti.
Iago traditionally lets out an evil laugh at the end of his Villain Song "Credo in un dio crudel" in Giuseppe Verdi's Otello, even though the laugh isn't written in the score or script.
In Baldur's Gate, Sarevok from the first game is available in the final expansion as an optional party member. His maniacal laughter is one of his battlecries and he'll sometime throw his evil laugh when you give him orders.
Gruntilda in Banjo-Kazooie. She gave out a rather impressive evil cackle whenever you hit the "Save and Quit" option in the menu.
In BioShock, Atlas/Frank Fontaine bursts into an Evil Laugh once you have killed Andrew Ryan and shut off the self-destruct. Arguably, this is also a moment of Nice Job Breaking It, Hero, although one built into the game's plot.
In what is probably the strangest example ever seen, the vending machines have a truly unsettling evil laugh.
Wait till you see the one in Hephestus that is broken and lobs grenades at you.
Any appearance of Big Bad Dr. Goldfire in the Blake Stone series is accompanied by his trademark "BWAHAHAHAHAHAHAA!"
Bagular's evil laugh in Bomberman Hero is...Well, it's weird, creepy, and strangely awesome at the same time. Seriously.
Then there's Rukifellth from Bomberman 64: The Second Attack, whose evil laugh can probably be considered a Verbal Tic.
Rukifellth: Once I regain my earthly body, I shall create a new cosmos. MY Cosmos. A cosmos of chaos where only the strong shall survive! Ha ha... Ha ha... Bwah ha ha ha ha ha! (5 second pause) It's not much fun making speeches about my plans without an audience...(pause) Ha ha...Ha ha...Bwah ha ha ha ha ha!
Castlevania's version of Dracula loves doing this, and since he's voiced by Norio Wakamoto, it makes it all the more enjoyable. It's also apparently a mandatory thing for the Lord of Darkness, because Soma Cruz does it, too.
Castlevania: Curse Of Darkness has Isaac, who has an amazing crazy laugh in both the Japanese and English voice tracks. The one in battle is bad enough (because he's about to kablam you across the arena with a certain attack), but in a particular cutscene (when Dracula brainjacks him and shanks Trevor Belmont), it's just - yeah. Don't play the game in a dark room when you approach that point. Isaac's English voice actor is, incidentally, Liam 'Also Lezard Valeth' O'Brien.
Dracula has a wicked snarly cackle in that game too.
Barlowe's laugh, especially when he frees Dracula's soul.
In Cauldron 2 for the Commodore 64, the witches have a good, hearty, and, by today's standards, painfully artificial sounding laugh each time the player dies. And it happens quite a lot, this being a Nintendo Hard game.
In Chrono Trigger, if you choose to sit down on the evil-looking throne in the Giant's Claw, really the Tyranno Lair long after Azala's defeat, the character in the front of the party does an Evil Laugh.
Wannabe mad scientist Vernon Von Grun frequently does this in City of Villains when you report back to him after a mission. It is made funnier by him asking you if you can tell that he's been working on the laugh, the laugh changing a few times, him asking you to join him in the laugh, and once breaking into a cough mid-laugh.
There is also an evil laugh emote for the players; though it lacks sound, it does go through physical motions that would make Doctor Frankenstein proud.
The evil AI CABAL in the Command & Conquer: Tiberian Sun expansion pack Firestorm will, occasionally, do the mechanized Evil Laugh in the middle of a mission to taunt you.
Kane himself indulges in this trope during the intro to the GDI campaign of Tiberian Sun. Being the Magnificent Bastard that he is, however, his laugh is a confident chuckle instead of a crazed cackle.
Tanya from the Red Alert series likes doing this a lot, and, in at least one promotion video of Red Alert 3, Emperor Yoshiro and Premier Anatoly Cherdenko do this together with Tanya.
Jedah of Darkstalkers is normally unflappable and very polite, but occasionally will indulge in a bit of mad laughter; most notably in one of his supers when he drags you underground and has hands of blood beat the crap out of you, or during one of his victory poses while gouging his eyes out!
It comes to the point that whenever Laharl shows up in the sequels, the first indication you have that he has appeared is his (very recognizable) Evil Laugh piercing the air.
Badly attempted by evil clones of Kurtis in the sequel, Disgaea 2. Given the fact that their speech is more stilted than William Shatner, it comes out as a robotic "HA hee HA HA."
Like Laharl, Mao, the main character of Disgaea 3, will actually begin his respective turn with a humorously quick but evil "Mwuhu-haha!" What make it funnier is hearing it from the same voice actor that does Edward Elric in the English dub of Fullmetal Alchemist, Vic Mignogna. In the Japanese, it's loud but simple "HA HA HA!"
Appropriate for one seeking to be a final boss, Desco of Disgaea 4 uses a short, simple "Fuhahaha!". The evil factor of it is watered down a bit by her cutesy voice, though. There's also Valvatorez, who uses a calm "Hm hm hm..."
Few have a more evil and constantly used laugh than Overlord Zetta, he will even laugh like a maniac during his signature Zetta Beam attack.
Honestly, anyone with a good enough title will be subject to this trope, especially the Overlords in the Disgaea series and Makai Kingdom.
Although he's pretty completely Lawful Good, Alistair of Dragon Age: Originscan do an impressive Evil Laugh anyway. (Unless he spoils it by coughing in the middle, as he does once.)
Played much straighter, some of the darkspawn have extremely deep, growling, guttural laughs.
The Mother does this at the tail end of her first cutscene in Awakening. It's definitely Fan Disservice and intentional horror because her bare breasts jiggle while she does it. All eight of them.
In Dissidia: Final Fantasy, he gets to laugh at the heroes together with Exdeath from Final Fantasy V during the opening of Shade Impulse. Kefka's high-pitched howling and Exdeath's deep belly laughter mix together well.
From Halo, there is but one maniacal laugh, but considering who it comes from, it's creepy. Y'know how, villainous as he was, the Gravemind always seemed fairly calm and convincingly sane for a monster in any previous talks or encounters? Well, all of that is thrown away right after the final obstacle to his plan dies, and he lets rip a spectacular one. It's even worse when you remember (and hear) that even his voice is composite in nature. Listen to it here, and crank up the volume after the 'obstacle' dies.
He has a knack for doing this when his victory is (supposedly) assured. The Librarian mentions that, at the climactic final point in the war against the Flood, the Flood were... flooding every channel they could find with laughter. Imagine... trillions and trillions of assimilated aliens of countless species all laughing with the distorted, horrific voice of the Flood.
Leopold Charles Anthony Weasleby the Third from Henry Hatsworth In The Puzzling Adventure is unique in the fact that his Simlish Speak is mostly compromised of variation of his maniacal cackle and sneer. Cole actually has a similar one, which is not surprising since Weasleby is actually a robot that Cole created to antagonize Henry.
The Commodore 64 port of the 1984 computer game Impossible Mission is one of the earliest examples of digitized speech in a game. The villain's evil laugh signified that the clock had run out and the game was over. (Conversely, if the player successfully completed the game, he gave a kind of Big "NO!".)
The Commodore 64 version of Ghostbusters also has a decent evil laugh upon loading, though the voice doesn't belong to any character.
Killer7. Every single enemy in that game has a nightmare-inducing evil laugh. Creepy, twisted, truly completely insane. Even their boss, Kun Lan, has a bit of the more megalomaniacal variety.
Xemnas also has a good one (Kind of requisite for any villain voiced by Norio Wakamoto), though in Kingdom Hearts II, it's mostly drowned out by the sound of the Beam Spam he's unleashing when he does it. You can hear it much more clearly in Dream Drop Distance while he's chucking buildings at you, though he doesn't always decide to use it.
When the surviving Organization members first appear to Sora & Co., they have a chorus of evil laughter at his expense. Most are pretty good... and then there's Demyx's rather unintimidating giggle.
In the first game, Gengar has his well known, "Keh keh keh!"
Although he mostly avoids this in all his other appearances, Albert Wesker can't resist letting fly with this in Resident Evil: Code: Veronica. He's definitely outclassed by the Ashford twins, though; both seem to have a rather serious, nay, terminal case of the sillies.
Paper Mario: The Thousand-Year Door has five villains with their own distinct laughs. Emperor Grodus' is something like "Gaaack aaack aaack aaack aaack"; Lord Crump' is a "Buh! Buh huh huh huh huh!"; and Beldam's is "Mwee hee hee hee hee".
Also, Doopliss's "hyuk hyuk hyuk".
And the Shadow Queen's "Muh huh huh huh huh".
Being who he is, Wario has been known to do this. He even does this in his first appearance (Super Mario Land 2), or at least that's what it's supposed to sound like.
WAH HA HA HA! AHH HAH HAH HAH!! WAH HAH HAH HAH HAH!!!! One of his victory taunts in Super Smash Bros. Brawl. Very nice, Wario.
Iori with Flames also laughs maniacally in KOF XIII whenever he completes his Shiki Saika combo, his Neomax also has him briefly enter the Riot of Blood state whereupon he laughs evilly and then crushes his enemy with a massive ball of flame.
Ryuji Yamazaki has a really crazy laugh that's heard in his winpose (example). 2002 Rugal has a good one, too, but it's to be expected since it's Norio Wakamoto. His original voice actor, Toshimitsu Arai, pulled off a pretty great one in '98.
Ganondorf has his fair share of laughs. Sometimes, it is a simple, self-satisfied "Hm-hm-hmm...", other times, it is used in a mocking tone after he has succeeded in an attack. One of the most flamboyant is in The Legend of Zelda: The Wind Waker where, after the King of Hyrule uses the Triforce to wish for "hope" for Link and Zelda and the old Hyrule begins to flood and vanish under the water, Ganon starts to laugh madly, his sanity broken at seeing all his dreams smashed before his eyes. The laughter continues to grow until he is eventually practically shrieking. Some found it quite silly, others thought it was pretty awesome.
Then there's his laugh in Super Smash Bros. Brawl. Specifically, in Subspace Emmisary. He's laughing from across a very shaky holographic interface, so his laugh dissolves into the most malevolent sounding static you've ever heard in your life.
Lampshaded in The Curse of Monkey Island. During Guybrush's first encounter with Murray, the Demonic Talking Skull, Murray busts out a laugh and Guybrush asks him about it. Murray (whom Guybrush had just blown apart) says that there's a fish nibbling on his foot somewhere and it really tickles.
Above lampshading happens in the first few minutes of the game. The trope is played straight in the rest of the game. He really likes to let us know who he is.
Murray: I AM MURRAY, THE EVIL DEMONIC SKULL! BWAHAHAHAHAHAHA!
King Andre, the smuggler leader of Skull Island, has a fairly impressive evil cackle that he breaks into for no apparent reason. Guybrush eventually asks him to stop, as it's fairly unnerving.
Klogg, the villain from The Neverhood, has a pretty awesome laugh, which not only appears in the Bad Ending (which manages to top it by having the hero, Klaymen, do an even better one of his own, after his mutation into a Klogg-like being), but in the opening credits as well.
Evil Witch/Warlock Sims in The Sims 2 had idle sequences in which they would cackle and Clasp Your Hands If You Deceive. In Sims the Third, if your character has the Evil trait, they'll cackle and wring their hands in the most satisfying way, point ominously at other Sims and gloat about...their impending doom?
The Guardian from the final Ultima trilogy had a particularly memorable, bass-pitched, resonating laugh, courtesy of Bill Johnson and a filter that made his voice progressively deeper in each of the games.
Also, blood elves, to some degree; the females especially sound rather psychotic. The male gnome and (ironically) draenei /laugh emotes (respectively a high-pitched cackle and a raucous guffaw) also sound a bit maniacal. And female trolls sound like the Wicked Witch of the West.
Due to the introduction of death knights, every race and gender can now have one, via an optional sound filter.
Sellers, the resident Mad Scientist, does one when Jr. and the others find out that Dmitri, Jr.'s crazy father, is going after the Durandal and its crew. It's not nearly as maniacal as Albedo's is, but it's still worth mentioning.
Crispin Freeman gets a chance to let out an over-the-top evil laugh as Heughes in Baten Kaitos Origins. He's not the only one, actually; quite a few of the villains in the series let out some seriously mad Evil Laughs.
In Donkey Kong 64, this is done by both the Big Bad King K. Rool and the insanely creepy third boss Mad Jack (who's laughter is part of the background music).
Oddly enough, it's also done by Bleak in Donkey Kong Country 3, when he's hit by one of the Kong's attacks.
The Mother from Dragon Age: Origins – Awakening delivers an impressive evil laugh in her first scene. She is initially distraught over the loss of "her pets" at Kal'Hirol, but calms down at the urging of a follower. When asked what she will do next, she shrieks that first she will laugh.
Dr. Curien, the staple Mad Scientist villain from the House of the Dead series, gets a rather memorable one in the third instalment. As the mid-chapter cutscenes up until that point had depicted him as a benevolent scientist seeking a desperate cure for his son's illness, once he lets the laughter loose, you know he's gone bonkers.
From Rumble! @ the Campus, we have the Shirogane family: Hayato, Hinako and their father Principal Hideo. Only the principal's wife Dr. Hikari Shirogane does not do this.
Hotaru Katsuragi and her evil laugh is enough for you to urinate in your own pants. Fo Sho.
Don't forget Dark Kiyo as well.
Wheatley in Portal 2 gets one, which seals his Face-Heel Turn, and later comments on how exhausting that kind of laughter is.
The defective turret you designate as the new master turret template will emit one whenever one of its faulty brethren makes it past quality control or when one of the functional turrets gets launched into the incinerator.
In Fist Of The North Star Kens Rage, quite a few characters possess evil laughs, which is not unheard of for the series. However, what is notable is that Souther/Thouzer not only has a spectacular evil laugh which can be activated at will, but that it's also weaponized. Tapping the special move button makes him cross his arms in a Badass Arm-Fold and laugh evilly, while spontaneously making everyone within about ten feet of him explode into bloody chunks without actively touching them.
The Prince in the early parts of Rule of Rose has an Evil Laugh that varies from quick giggle to truly psychotic cackle as he has Jennifer in his power. He becomese much more subdued later on, however, as his role in the story becomes more ambigious.
The Prince: For I am the Prince, and the Prince rules! Mwahahahahaaa! This is your life, but you're playing by my rules! Hahahahahaaa!
Colonal Bahamut in Contra: Hard Corps and Master Contra in Neo Contra have this in their first appearances.
Wild Dog laughs in every appearance in the Time Crisis games.
General Morden, the Big Bad of the Metal Slug series, and his Dragon Allen O'Neal usually laugh upon confronting the player.
The first three Syphon Filter games ended with a laugh from Mara Aramov.
Streets of Rage has Mr. X laughing whenever the he struck the player down or if he or she lost a life while fighting him.
Dynasty Warriors and Samurai Warriors both feature some characters sporting some pretty evil laughs. While one could probably credit Oda Nobunaga with a pretty cold chuckle, it's Sima Yi of Wei who takes the cake, with a gleefully wicked laugh from practically every person who's voiced him. Probably achieves its height in Dynasty Warriors 5's Expansion Pack, where he cackles loudly and dismissively at an enemy general's corpse in a cutscene, and several of his moves are punctuated by evil laughter and constant use of the word 'fool.'
After defeating Neff in Altered Beast, every boss battle with the exception of the last ends with him stealing your power orbs and laughing maniacally.
Walter Sullivan from Silent Hill 4 laughs before, during, and after he attacks you, as well as when you are attacking him.
Angry Birds Star Wars for some reason gives the Darth Vader pig one, even though the actual characters isn't heard laughing. Does this most times he appears even if he's defeated.
In Star Wars: Empire at War, the ending cutscene for the Imperial campaign has this from Emperor Palpatine (naturally.) Lampshaded by the subtitles:
Emperor: *Evil cackling laughter*
Grandia 2's Melfice loves to laugh evilly. So much so that in battles with him, he never actually stops laughing.
During the ending of Marooned the villain Radimar had an evil laugh which lasted so long and had such an overabundance of reverberation at the end that it quickly descended into Narm territory.
XCOM: Enemy Unknown: Your psionic troopers give bloodcurdling ones when they use Mindfray. It either makes you chuckle along with them or makes you wonder if Dr. Shen was right...
Iggy's Reckin' Balls has both Narlie and Skully. Narlie's laugh is a very deep "Huh huh huh huh...", while Skully's is a high-pitched, fast cackle.
M in Shikkoku No Sharnoth only ever emotes when he has destroyed one of the Metacreatures. When he does, he launches into a full blown evil laugh. Mary is quite disturbed.
Dr. Shuu in Hatoful Boyfriend has a distinctive "Ho ho ho ho ho..." chuckle. Which is actually pretty decent onomatopoeia for the real call of a Chukar Partridge.
In the Halo 3 machinima, Deus Ex Machina, a villain starts laughing because he's supposed to be a robotic time-gasbomb; when it turns out to be a dud, he plays a looping laugh sound. Yes, it is creepy.
Another earlier villain chuckles to himself in a completely normal manner. Since he is laughing about the hero's imminent doom, it still feels very evil.
Damien from El Goonish Shive does one of these. Not because he's just done something evil, or because our heroes are falling into his diabolical trap, but because he thinks it's genuinely hilarious that Grace could ever be a threat to him.
Likewise, Raven has a pretty well-developed evil laugh.
Made creepier by him turning out to actually be good.
The more villainous and/or powerful "Sparks" (genius gadgeteers and mad scientists) in the gaslamp fantasy world of Girl Genius ("Adventure! Romance! Mad Science!") are remarkably prone to this. But then, frankly speaking, every Spark is prone to ranting and raving and overdramatic gestures (often involving blowing things up or, sometimes, coffee) when "in the Madness Place" (sparking at full throttle). "Forward, my minions! Mhuahahaha!"
Narbonic loves this trope. At one point, Helen gets a manic giggle: "Bwee-hee-hee!"
TAG of Schlock Mercenary has one of these built in as part of the general psychological warfare suite he is equipped with. It activates involuntarily, whenever he thinks he has complete power over someone (it doesn't really matter if they're in the room or not).
Another of the trolls, Terezi, is almost always seen giggling evilly, not helped by the fact that, the first time she's introduced, she's giggling after threatening to kill John. As it turns out, Terezi isn't really evil, just very passionate about justice, as well as being a very strange and funny girl in general. H3 H3 H3!
Do honks count as laughter? HONK honk HONK honk HONK hoooooooooooonk ;OD
Zoophobia has KayCee's "Hm hm hm hm ha ha ha HA HA HA HA HA HAHAHAHAAA!"
The Last Days of FOXHOUND: In a surprisingly low-key version of this trope, Jim Houseman can't help himself but let out a "Mwaha" after every time he says something that indicates that he is Obviously Evil.
Dr. Horrible opens Act I with his unbelievably artificial maniacal laughter. Hey, he's working on it (with a voice coach).
For his views on the evil laugh, see the quote page.
He gets it extremely right in Act III.
And then there's Bad Horse, the leader of The Evil League of Evil, with his "terrible death whinny".
From Gaia Online. Johnny. K. Gambino. Lampshaded in a later story update where Johnny, stuck in a rut, says he just doesn't have the passion for the Bwahaha stuff anymore. Another lampshade happens when the Sentinel uses the same laugh, and Johnny complains that she's stealing his routine.
Among the site's staff, Qixter, the main dev for zOMG!, makes himself out as an Evil Overlord, complete with Evil Laughter. His trademark quote is "Can I get a Bwuhahaha?" This quote makes it into the description of the item at the end of the game.
Parodied by Adam in Maddison Atkins episode 9, and also demonstrated by Maddison in episode 38.
Nostalgia Critic: Hey, look, I found a credit card. (Beat) MUAHAHAHAHAHAHAHAHAHAHA!
Mara Wilson pulls off an epic one in the review of A Simple Wish while antagonising the critic with embarssing home videos of himself after he criticised her acting. Heck she even gets red ,demonic eye glow
Dr. Insano: You can't just start laughing maniacally at the end of every sentence! You have to earn it! You have to say something dramatic, like "soon we will conquer Thailand, and tomorrow THE WORLD!"
Dr. Nye:(in movie) Today we conquer Thailand, and tomorrow THE ENTIRE WORLD! HAHAHAHAHAHA!
(Insano, Spoony, and the Spoony Bum start laughing maniacally)
Dr. Insano: SHUT UP!!!
Played straight and lampshaded in the Whateley Universe story "Test Tube Babies" when the woman playing the bad guy in the holographic simulation turns out to be a real retired supervillainess who terrorized three continents during the Cold War.
"But of course, Herr Bardue. In the last sim, I didn't even haff time to give zem my evil laugh.” She clenched her fists and looked at the ceiling. “BWA-HA-HA-HAH!"
The Quintessons during the "Five Faces of Darkness" arc were initially so overcome by their moment of triumph, they were at a loss as to how to go about celebrating it. Then one of them suggests a quiet chuckle. And so they do, and rather creepily.
The Quintesson judge in the movie pulls off an exceptionally creepy five-faced laugh after the hapless robot he'd declared innocent is fed to the Sharkticons.
Because of his normally almost emotionless demeanor, Soundwave very rarely laughs in the original series. When he does, though? With his flanged, echoing voice? It is awesomely creepy.
Beast Wars has a number of the Predacons do this, including, but by no means limited to, Megatron when he's feeling smug, Rampage when he's inflicting pain, and Tarantulas almost constantly. They're always the third sort and always a pro at it. Inferno does it a lot too. Mostly because he's completely Axe Crazy.
Lampshaded near the end of "Other Visits Part 1", where both Megatron and Tarantulas begin cackling at the same time after coming together to work on the same Evil Plan. Hard to describe on paper, but plays out very nicely.
Megatron in Transformers Armada is the biggest offender, though: he laughs a lot, never passing up an excuse to do so. As the Big Bad, he has that right, but the Cybertron version, played by the same voice actor (who also played the Beast Wars Megs), doesn't laugh nearly as much.
Kim Possible: Seńor Senior Senior views the Evil Laugh as a vital part of villainy. When Seńor Senior Junior states this view to Shego (in "Two to Tutor"), she tells him that his father has "warped his mind."
Then there is Ron's evil laugh after he turned evil
Lisa Simpson has her own share of evil laughter in a few episodes. In "Girly Edition", after elaborating a scheme against Bart, Lisa has an evil laugh which was comically followed by Homer's monkey helper's own evil laugh, creeping out Lisa. Also, in "Last Exit to Springfield", after she has braces installed, leading to a spoof of the Joker's laugh from the 1980's Tim BurtonBatman film.
Marge Simpson has a more raspy one in "All's Fair in Oven War" in which she sabotages her opponents' food with Baby Ear Medicine. Also, she gives one in the third segment of TOH IV in which she reveals that she's the head vampire.
Even Homer has one in a few episodes, such as "Flaming Moe's", "When Flanders Failed", and "The Fat and the Furriest" when he makes a big cotton candy ball with caramel on it. castle thunder also helps up the Frankenstein parallels.
"Bart Sells his Soul": Milhouse has one in this episode when begged by Bart to give him his soul back, but with a price: fifty bucks.
"Whacking Day": Skinner has one after tricking Bart, Jimbo, Nelson, and Dolph to receive mountain bikes only for him to forget to turn off the microphone, causing everyone to hear.
Groundskeeper Willie has one in the second segment of TOH VI, "Nightmare on Evergreen Terrace".
Hank Scorpio definitely gives one in "You Only Move Twice".
Comic Book Guy has his own evil laugh in the classic episode "Three Men and a Comic Book."
The Smurfs: Gargamel and...his pet cat, Azrael. Usually, the two laugh – master first and cat next – after Gargamel consecrates his latest scheme to capture and/or kill the Smurfs.
Lampshaded in Tubbimura's first appearance, when he engages in a Showdown with Raimundo. Jack, who is watching, is anxious to see just how good his evil laugh is. (Tubbimura actually has a pretty good one, but he loses the Showdown.)
Every time the villain The Terror attempts one he ends up in a coughing fit due to his advanced age, his villainy dated back to the 1930's or even earlier.
In the Tom and Jerry short Solid Serenade, Tom traps Jerry in Spike's doghouse and gives the audience an Evil Laugh as he closes the door. Then Jerry emerges, being safely escorted out of the enclosure by Spike himself...who utters an Evil Laugh of his own as he shuts the door again. Hilarity Ensues, natch.
In Jerry and the Lion, Tom traps Jerry in a closet and again gives one of these to the audience as he closes the door...not realizing that Jerry is accompanied by the escaped circus lion who he's befriended. Hilarity once again ensues.
Dick Dastardly in Wacky Races (and everything else he's been in for that matter). In this case, though, it doesn't make him threatening at all.
Nox, the Big Bad from Wakfu, can sometimes get into a pretty demented Evil Laugh to conclude some of his monologues.
It's referred to as his "psychopath laugh" in this convention video with the voice actors (a demonstration is given 40 seconds in.)
Season 2 Big Bad, the Eliatrope Qilby, is no slouch in this department either. Since he actually has a visible face, unlike Nox, his Evil Laugh looks much more demented.
Thailog from Gargoyles has a good one; the comic continuation of the series shows that he gets disappointed when he goes for long periods of time without a chance to indulge in this, and finally having an excuse to do so cheers him up immensely. When he is initially introduced and people wonder if it is Goliath (who Thailog is cloned from), Elisa doubts that the heroic and straight-arrow Goliath even knows how to laugh maniacally. Hudson even directly asks Goliath: "Do ye even know HOW t' laugh maniacally?"
Demona loves this trope.
Jonny Quest episode "The Werewolf of the Timberlands". Pierre (the "werewolf") has one.
Invader Zim has Tak, Zim's far more competent and villainous rival, giving an evil laugh that lasts more than 10 seconds, followed by still more evil laughter as she goes over her Evil Plan. Zim doesn't quite get it, despite having his own evil laugh, and responds with "yes, yes, I'm a master of comedy".
Zim himself plays it straight; after a plan to get rid of Dib appears to be successful, he struggles to find a sufficiently evil way to end his monologue.
Zim: Now Dib, I leave you to your... eh... GIR whispers "say 'moosey fate'!" Zim follows the suggestion, looks like he can't believe he just said that, then nonetheless bursts into explosive evil laughter.
"Muhahahaha! Mhahahahhaa! MUHAHAHAHAHAAAA! HA,HA,HA,HA,HA- *ahem*... I LOVE EARTH!"
And when Dib thinks he's getting one over on Zim, he's not above an evil laugh or two himself.
Discord actually has two distinct ones — his normal, evil, and somewhat menacing laugh, and his sinister-as-all-get-out laugh. The latter happens more often when he's not onscreen. On-screen, he may switch from an evil laugh to rolling on the ground laughing, still sort of evilly because his amusement generally involves someone else getting hurt.
The Changeling Queen likes her evil laughs as well. They sometimes verge on being mere evil giggles when she's using Princess Cadance's voice.
Fluttershy in "The Best Night Ever" when she snaps and temporarily goes yandere. She just wanted to play with the cute animals...
"I'll get you yet, my pretties. (Twitchy Eye) Oh, yes. As soon as one of you little birds, or monkeys, or bears touches this net, you'll be mine! Mine!! Hahahaha! Hahhahahaha! Ahahaha — whoa!" (Gets caught in her own net, goes back to looking cute.)
Adventure Time: Princess Bubblegum pulls off a pretty insane evil laugh in "The Duke" when she thinks she finally has an excuse to lock up the Duke of Nuts. In "Mortal Folly", the Lich starts laughing when Finn is about to defeat him with a sweater.
When Anakin takes off on Dooku's speeder bike to save Ahsoka in the pilot movie, Dooku is said to be "(LAUGHING MALICIOUSLY)" according to the subtitles.
In the Mon Calamari arc, Riff Tamson seems determined to laugh evilly once every 5-10 lines of actual dialogue he has.
In the Umbara arc, General Krell spends the first three and a half episodes as a gruff, domineering officer. However, once he admits that he is revealed to be a traitor, he laughs deeply in nearly every following conversation.
Ed, Edd n Eddy: "You've met your match, Melon Head! Prepare for... PROFESSOR SCAM!!" MUAHHAHAHAHAHAHAHAHAHAHAH!!!
Edd:...He's lost it. Honestly?
Makuta Teridax from BIONICLE has a very deep and evil laugh which he performs quite frequently.
Ren Hoek in "The Ren and Stimpy show" is often prone to crazed gigging.
Stripperella. A Mad Doctor lets rip, then shuts up when he realises he's got a waiting room full of patients staring at him.
Dan Vs.: Dan has a wicked evil laugh as heard in some episodes. Dr. Pullum, his supervillain dentist, sports one, as well.
Super Friends. In the 1973/74 episode "The Menace of the White Dwarf", Raven has one, which he uses after he steals the Washington Monument and brings it to his cloud base.
Tom Cruise, in his infinite wisdom, suddenly went bonkers in an interview with him that veered into a discussion of his place in the Church of Scientology. Amongst "pew-pew"esque noises directed at "SP's", the man began laughing crazily. Naturally, the Church tried to get the video taken down, however...