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Axis Powers Hetalia
  • Gankona, Unnachgiebig, Unità: After Germany pulled out a Browning Hi Power on Japan and Japan pulled out his katana on Germany, Italy screamed one word for them to stop.
    "NOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!"

The DCU

  • In Hellsister Trilogy, Linda Danvers introduces her new beau Dev-Em—who is secretly a 31st century Legionnaire—to her landlady Ida Berkowitz, who grows concerned with Linda dodging every question regarding her new boyfriend's mysterious personal life. So, when Ida asks if Linda could take her to his workplace...
    Ida: Seems to be hiding something. 'Security consultant'? So all right. Maybe he is. Maybe he's got to be Mr. Secret to do his job. You sure this is really what he does?
    Linda: Sure as I can be.
    Ida: Like how? You been to his place of business? Could you take me?
    Linda: NO! I mean, yes, I've been there, and no, I couldn't take you. He made an exception for me.
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  • This is Damian's reaction when he's told Bruce and Selina are expecting a baby, early in Batman: Grudge Match. At the start of the next chapter, Alfred is sweeping up glass from a window, implying Damian's scream shattered it.
  • With this Ring... (Green Lantern):
    • Exclaimed by Hector Hammond when Hal Jordan takes advantage of his low mental defenses to escape his death trap and retaliate.
      Then he felt a pressure in his own mind. A pressure which rivalled any to which he had subjected a foe. The mentalist's defenses went into operation, but it was a bit late. After all, when an enemy has transformed his body into an analogue of mental energy and sped up the telepathic pipe into your mind, such an action is closing the gate once the horse has bolted.
      <NO!>
    • Screamed by the Predator when he gets blasted into oblivion.
      A purple burst of light cleaved through the roof of the throneroom, smashed into the force-ball, and crushed it into the Predator's body.
      A light of terror went on in his eyes as he shouted, "NOOOOO!", before finally and totally combusting out of existence.
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Dragon Ball

  • In Dragon Ball Z Abridged:
    • From episode 10:
      Goku: Kaioken...
      Vegeta: Noooo!
      Goku: ... times...
      Vegeta: No no no no!
      Goku: ... FOUR!
      Vegeta: No no no no FUUUUUUUUUUUUUUUUUUUUUUUUUUUuuuuuuuuuuuuuuuuuuu...!!!!!
    • Additionally, Vegeta's response to Ghost Nappa.
    • Plus there's Mecha Freeza's reaction to learning that he blew up Planet Namek and the dragon balls from King Cold, in a scene that is very much like Vader's in Revenge of the Sith.

The Legend of Zelda

The Loud House

Marvel Universe

  • In A Prize For Three Empires, Carol Danvers lets one out, combined with remorseful tears, when she finds out Zenith, a Shi’ar guardsman she killed during a battle, was her friend Raza’s brother:
    Warbird looked up, her eyes closed and tearing, her fists clenched, and shouted one great word that filled the cabin.
    "NO!"
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  • In X-Men: The Early Years, Iceman gets one:
    "Are we there yet?" Bobby Drake whined for about the thousandth time. Scott Summers just sighed. Hank McCoy rolled his eyes. Warren chuckled humorlessly to himself and Jean was going to start losing her temper.
    "NO!" Was the reply that quickly came from all his teammates.
  • X-Men 1970: Uttered by the leader of an extremist group when Cyclops asks if fear of being conscripted is his chief motivation.
    Cyclops: So, you're doing this because you're afraid of the draft?
    Carter: NO!

Neon Genesis Evangelion

  • A Crown of Stars: Asuka combined this trope with Rapid-Fire "No!" in chapter 5. When Asuka insists that she does not need anyone and she has an agreement, Daniel points out that it is very fragile and she fears that it will not last... And then what? She will try to kill herself again?
    She hadn't cried, not even the day they buried her Mama. She would not cry now. She would not. 'Don't take him away. I don't want to be alone. I don't want to be alone.' "I don't... ...need anyone. My arrangement-"
    "Is a hollow shell, that you both fear and expect to collapse at some point. Then what? Water again? Or this time the barrel of a gun in your mouth?" Daniel said quietly, his words hitting Asuka harder than if he’d been shouting them. "Or a twist of hemp like your mother, feet in the air?"
    "NO! Nononononono! I...I...STOP IT!"
  • Advice and Trust: In chapter 4 Asuka shouts one when a classmate severely misunderstands Rei:
    Rei:"Though I believe this bond predates that session. Ikari is the first person Pilot Soryu ever let inside her Entry Plug."
    Tanaka:"Is... is she talking about-"
    Asuka:"NO!"
  • Evangelion 303: Asuka screamed it several times after Jessika told her that she could not be with her yet.
  • HERZ: Exclaimed by the SEELE members when the Children took control of their newest MP-EVAs.
  • The One I Love Is: When Shinji saw the MP-EVAs had chopped Unit-02 into pieces and possibly killed Asuka he screamed a very big and anguished "no":
    I opened my eyes and then saw a scene that will probably haunt me the rest of my life. I saw nine white EVAs, circling around what was left of Headquarters. And in their hands, or even worse, their mouths, they held the remains of what had once been Unit-02.
    "NOOOOOOOOOOOOOOO!!!!!!!!!!"

Sonic the Hedgehog

Yu-Gi-Oh!

Unsorted

  • In Tales for the Leet's Romeo and Juliet, Juliet has an especially Big No after finding Romeo dead, taking three lines of dialogue to complete and ending with multiple !s and 1s.
  • z3ll58 does a very good and long No in his "Let's Play Final Fantasy 6" when Leo dies.
  • The beginning of Christian Humber Reloaded, when the title hero sees his owners killed. He then follows this up with "WHOEVER DID THIS WILL PAY DEARLY, BECAUSE I LOVED THEM?"
  • Happens at least once per chapter in ''Light and Dark The Adventures of Dark Yagami, since people are constantly "sexing" and dying and coming back to life and dying again.
  • StarKitsProphcy has one of these about once every other chapter later in the story. Or, due to Rouge Angles of Satin, a big "MOOOOO" or "OOOOOO." Or possibly "NOOOOOO999999999 OOOOOOOO". Or, in one case, "IIIIIIIIIEEEEEEEEEEEEE !1!", apparently as Gratuitous Japanese.
  • In A Day in the Life of a Commissar, a Dawn of War Machinima, Commissar Steeve does this when he's told Galactic Starbucks is out of coffee. He stops to ask "Really? What kind?" once he's told that there is, in fact, some coffee left. And finishes when he's told it's decaf.
  • In Deserving, everyone has No Indoor Voice especially when uttering the word "no", which appears in ALLCAPS as often as not.
  • In Revenge Road, Kyosuke wakes up after realizing that the previous events, Hikaru killing him and Madoka was All Just a Dream. Madoka then gets up to answer the door, like she did before, and when Kyosuke realizes what time it is, he runs down the stairs yelling "Midnight? Oh no! Madoka! StOP! NOOOOooooooo!", only to find that he is too late as Madoka gets shot and the fic ends.
  • In The Rob Chronicles Rob does one when Chrome dies.
    • And again when Web dies.
  • In Modurn Warfare 3 The Dead Rise, Qwaritch gets an extremely long one as he sees Jak die, and Ian Hawk gets one when he's decapitated and eaten by a fellow prisoner.

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