Entirely unintentional example in Luminosity between an old vampire named Addy and five-year-old Elspeth. The author delayed the next update slightly to edit that particular ending away, but the original ending line of Radiance, chapter 14, was:
Sokka and Zuko in Embers. On the beach, they have very similar reactions; they really seem to understand each other, much better than most people; they trust each other, and neither Sokka nor Zuko is naturally trusting; they know that they both have good intentions and respect that, even when they're in conflict; unlike with Toph, Zuko and Sokka are of similar ages.
The Harry PotterFan FicThe Girl Who Lived. Rose swears up and down that she's not a lesbian or bisexual, but spends much of her time describing girl's bodies primarily in the order of breasts, nipples, pubic hair, rest of their body.
Total Drama Comeback Series: Due to Author Appeal, there are strong Bridgette/Gwen undertones in the series, despite the two already having boyfriends and receiving plot-relevant Ship Tease with male characters. In two separate challenges, Gwen kisses Bridgette on the lips very passionately and without hesitation in order to complete a challenge, and Bridgette accepts and indulges in it longly and deeply after the initial shock at the sudden nature of both kisses wears off.
In the Backyard Sports fanfiction ''The Secret Life of the Backyard Kids", Jorge and Tony are "as close as male friends can be in a children's story."
In the Alexandra Quick series, there is so much Ho Yay between Alex and Anna. One: They're dorm-mates. Two: They cannot stand when one of them is harmed by an evil entity. Three: At the end of book four, Anna declares platonic love for Alex. Except it might could possibly not be platonic....
Special mention goes to Ry. The boy considers all males his friends just because they're the same gender, for Christ's sake!
A lampshaded version occurs in Clothes, please?, when Ren mocks Pyrrha's inability to confess her love to Jaune.
Still, just saying, if I were Pyrrha, I would have taken Jaune by his scraggly blond head, dragged him back to the room and madedamnsure he had no one else on his mind...No homo.
In Angel of the Bat, Cassandra Cain and Stephanie Brown have some Les Yay moments (ironically while reading the Bible together) but it's not meant to be much more notable than a lot of their time hanging out in the comics. Ultimately subverted and even ends up being a plot point- Cassie does have romantic feelings for Steph, but they're unrequited.
Kitsune no Ken: Fist of the Fox, a NarutoHigh School A.U. story, gives us Hinata and Yugito; the one is a sheltered Lonely Rich Kid, and the other is a former Fair Cop and a member of the infamous Nine Terrors. Although both of them are confirmed as straight, the Les Yay vibe can be seen here and there between the two of them, with the very first instance of it being shown when Yugito, a self-admitted Shameless Fanservice Girl, takes a bath in an artificial waterfall on the balcony of her hotel suite with the express intention of shocking Hinata's genteelness when the younger woman comes to visit her, and Hinata, after some initial surprise at the sight, spends the next few minutes checking out Yugito's ample body and comparing it to her own. Much later, in the story's Gaiden chapters, Hinata reveals that she's got a bit of a playful side of her own, as shown in the first Gaiden where she gently tickles Yugito's bare feet while the woman is sleeping, and the vibe continues in the seventh Gaiden when Hinata eyes Yugito's body (particularly her boobs) up and down while Yugito's getting ready for her wedding. In the latter instance, Yugito actually catches Hinata looking and decides to tease her a bit more.
Yugito: See something you like, dear?
Career Assassin Miss Alice Band and others, in the Discworld writings of A.A. Pessimal. Alice has a special soft spot for boyish Thieves such as Steffi Gibbet, but a high-wire artiste and a complicated relationship with a Seamstress also come into it.
Thiscrossoverfanvid is about Belle becoming close to a werewolf woman named Esmeralda. It's supposed to be platonic, however it's hard to remove Esmeralda's flirty nature even with editing. It doesn't help that it uses many Paranormal Romance tropes.
Miya and Madame Boss have some unintentionally romantic-laden elements in Team Rocket Roots. Madame Boss is so close to her star agent Miya that she undergoes a Sanity Slippage when Miya disappears on a mission. When Miya dies for real, she thinks about how she'll be Together in Death with Madame Boss.
This occurs at the start of this fanvid by TheNight130 that crosses over Cinderella and Sinbad: Legend of the Seven Seas. Marina is supposed to be jealous of her friend Cinderella, but it instead looks like she's jealous of Cinderella's husband. It isn't clear until a minute in that she's in love with the prince.