Mother Gothel from Tangled is stated to be several centuries old.
Although WALLE is seven hundred years old, he is actually still working even after the day humanity left Earth. Somewhat justified in that he's seen replacing damaged parts using components from other robots of the same model, of which there are hundred or thousands lying around. It's likely he's replaced every part of himself many times over, save for his motherboard, RAM and memory.
Crush from Finding Nemo is a hundred fifty years old, and is still young.note Truth in Television for large turtles; even snapping turtles can live over seventy-five years, and tortoises and sea turtles have been known to live to 200.
Sheriff Woody from Toy Story just happens to be the leader and the oldest member of Andy's toys (he was created in the 1950s). Jessie, Bullseye, and, Stinky Pete, who are part of Woody's Roundup show, are on the same box (though since Stinky Pete appropriately looks like a grandpa, he can be considered an aversion).
Kida in Atlantis: The Lost Empire is said to be old enough to forget her original written language. Milo estimates her age as somewhere around 8,500 years. She replies "yes", as though this isn't anything to be surprised about. One Atlantean year is equivalent to 300 human years. This would mean that if Kida's age was compared to that of a normal human's, then she would approximately be 28 years old.
Jack Frost in is at least 300-years-old — but he barely looks past his late teens. He sacrificed himself to save his little sister as a human — because of this, he gained immortality and the power to control ice and snow.
All of the Guardians are even older than Jack, who is the youngest member of the group. While Santa, Easter Bunny, and Tooth Fairy all appeared in historical times, it seems that the Sandman has existed since humans first started to dream, as has his Evil Counterpart, Pitch, putting them closer to Time Abyss territory. Then there is the Man on the Moon, who is probably as old as the moon itself...
Minions hints that at least some of the titular creatures may be as old as the Cretaceous period, if not older.
An Angel for Christmas: It's strongly implied that Angela, a little girl, and Wilfred, a wolf, have been alive at least since the birth of Jesus Christ. Justified, being that Angela is an angel, and all.
The LEGO Ninjago Movie: Master Wu is 167 years old according to Cole, and he's considered the younger brother of Garmadon, who claims to be 174 years old.
SHAZAM!: The Wizard who transfers his powers to Billy Batson is old enough to remember Black Adam's rampage millennia ago
Darkseid (as Uxas) led the Apokoliptian forces in ancient times and rules Apokoplips thousands of years later.
Dorian Gray, in the film version of The League of Extraordinary Gentlemen. His exact age isn't given, but curmudgeonly old Allan Quartermain (played by Sean Connery) mentions that as a boy, he saw Gray giving a lecture at Eton College. In the original novel, Gray is never said to be immortal or invulnerable; he just retains his youthful appearance.
In 7 Faces of Dr. Lao, the eponymous doctor is technically an example of this trope, in that he looks rather old, but not nearly his true age of seven thousand, three hundred and twenty-two.
John from The Man from Earth looks like your regular guy, not like the 14,000-year-old caveman he actually is.
Elrond is featured in the opening prologue, which happens three thousand years before the story proper starts. When we meet him in the "present", he looks exactly the same.
Galadriel (as explained in the books) is even older; she's the only Elf remaining in Middle-earth who is mentioned before the first rising of the Sun, about seven thousand years ago. She's played by Cate Blanchett, who was in her early thirties at the time of filming.
Arwen was 2901 years old when she died. She didn't look any older than her mid-twenties. Likewise, Legolas was probably around her age when the Fellowship was active, and... well, there is a pattern.
The Wizards (and Sauron) are all Maiar, and so are literally older than the universe, having sang it into being.
More a case of Older Than They Look, Aragorn: in an Extended Edition scene from The Two Towers, Eowyn is surprised to learn that he is actually 87 years old. He doesn't look ageless, however: he looks like a very fit and active man in his late 40's, his hair flecked with a little gray. This is because he is a Dunedain, "blessed with long life" - but not eternal life. The books explain that the Dunedain live about three times longer than regular humans (Aragorn ultimately lived to be over 210 years old).
Thor: Asgardians can live hundreds, if not thousands of years and still be physically capable of feats of strength. The same thing applies to Frost Giants. If Loki was born about the same time as the battle in the prologue, that makes him over 1040 years old. In the sequel, he comments that Asgardians can live for approximately 5000 years. In Infinity War Thor states he's 1500 years old, Loki is likely about the same age given they grew up together.
Thor: Ragnarok: The Grandmaster says he would be considered millions of years old on any other planet. On Sakaar — surrounded as it is by time-warping wormholes — he appears to be merely a Silver Fox (he's played by Jeff Goldblum, who was in his mid-60s at the time).
Avengers: Infinity War: Red Skull has been living in Vormir for more than seventy (Earth) years and still looks the same as he did in Captain America: The First Avenger. Compare this with Steve and Bucky who are merely Older Than They Look since it's still possible for them to live today. Red Skull, on the other hand, was already a middle-aged man back then; he would be something like 120 years old today.
Speaking of Infinity WarBig Bad Thanos was confirmed to be well over 1,000 years old, way older than Thor and Loki. Although age doesnt seem to slow him down in the slightest, if anything just makes Thanos more deadly as he puts his millennial of experience to good use when thrashing the heroes.
Transformers: Revenge of the Fallen: Alice, who at first glance looks like a human college student who's in her early 20's at most. In reality, she's actually at least several millennia old, being a Decepticon Pretender.
Monk: She's 3,000 years old. Kull: She said she was 19!
In the film version of The Green Mile, Mr. Jingles the mouse is still alive, albeit very elderly, after an unprecedented sixty years. Mice normally only live for one to three years, making the little guy older than Methuselah when adjusted for species. Paul Edgecomb is 108 years old at the end of the movie, which is nothing too impressive yet, but the implication is that he may live to see his thousandth birthday, a thought that keeps him up at night.
The 2000 Dungeons & Dragons film has this interaction between Snails and Norda the elf, when Snails is chatting her up:
Norda: How old are you? Snails: Twenty-three. Yeah, I know I'm a little young for you, but what if I get my hands on an aging potion, huh? I'll sacrifice a couple of years for you. Norda: I'm two hundred and thirty-four.
In The Santa Clause, all the elves are played by children, one of whom informs Scott that she has "pointy shoes that are older than you". Judy, one of the head elves, looks to be about ten years old. When she mentions that it took her 1,200 years to perfect her cocoa recipe, Scott Calvin/Santa Claus tells her, "You know I must say, you look pretty good for your age," to which she hilariously responds, "Thanks... but I'm seeing someone in wrapping," eliciting a stare, a look of shock and a silently-mouthed "ooookay..." from Scott/Santa.
The Tin Drum: The main character, Oskar, willingly stops aging, resulting in an adult male in the body of a toddler. Towards the end of the film, he meets a dwarf who appears to be an adult, but reveals that he's really close to one hundred years old. Like Oscar, he decided to stop aging, and says there are others like him. They mention that they could essentially become immortal if they wanted, invoking this trope.
Wolverine in the X-Men Film Series, because of his Super Healing Factor. He was born in 1823, but as of today, he still physically appears to be a buff as hell mid-to-late 30s. His powers constantly rejuvenate his cellular integrity rendering him seemingly ageless. He's not by any means immortal, he just ages at an incredibly slow rate, if at all.
Pai Mei from Kill Bill, maybe. If the story about him causing the Shaolin massacre in the year 1003 AD was completely true and not a case of In-Universe case of Memetic Mutation, yes.
Andre Linoge, from Storm Of The Century. It's never made clear how long he's been around, but it's implied to have been at least since Biblical times. Toyed with in that, though he is old and still has at least another century to live (stating to the town that he will still be alive when the youngest among them has grown old and died), even others of his kind consider him to be nearing death. Which makes sense; if you've been around for at least 1000 years, another 100 is going to seem like just a few months.
Vampires and Lycans in the Underworld series can be centuries old without looking it. The main character, Selene, is explicitly stated to be roughly 600. None of the others have their ages stated even in ballpark figures, though it's obvious that most, if not all, of the other named vampires and Lycans are at least older than Selene.
Miriam and John in The Hunger, since they're both vampires. She turned him, and the trouble in the movie starts when his years begin to catch up with him, as eventually and invariably happens to all her companions...
Yoda: When 900 years old you reach, look as good you will not, hm? [chuckles]
The vampiric Anzati, who appear in many Expanded Universe novels, are an entire race who qualify. They can live to be up to a millennia.
Special mention goes to Chewbacca, who by the time of A New Hope is 200 years old.
The giants from Jack the Giant Slayer are long-lived, with many being the original invaders during their first invasion of Earth.
Near the end of the first Blade film, the titular character discovers that his mother (Vanessa Brooks), who he thought died during childbirth, is still alive and is a vampire, not looking a day older from when she gave birth to him. Indeed, her actress is actually 9 years younger than Wesley Snipes, Blade's actor, and one year younger than the actress playing Dr. Karen Jenson, the film's female lead who reminded Blade of his mother.
Additionally, his nemesis Deacon Frost turns out to have been the one who attacked Blade's mother all those years ago, while he looks to be about Blade's age. Of course, since the films are about vampires, it's pretty much a guarantee that this trope will be in effect for a good number of them.
The titular character in Dracula 2000 is significantly older than Bram Stoker's version, being about 2000 years old, as he's actually Judas Iscariot.
All the Abrasax siblings in Jupiter Ascending, and potentially any monied character Jupiter encounters in space. Kalique in particular just turned 14,004 years old and her mother was in her 91st millennium when she died (and not of old age).
The Captain Ersatz for Vulcans in the german comedy Traumschiff Surprise can get up to 400 years old. It's how Mr Spuck is still alive after they jump from the 21st to the 24th century.
Mr Spuck: I am a Vulcanette Vulgaris and they can age to 400 because they belong to the same genus as the Galapagos Tortoise!
The main character of The Last Witch Hunter is eight hundred years old in the present day part of the plot, thanks to immortality he gains in the flashback part. He has surprisingly little trouble fitting in with modern society.
In Time: The aging gene has been disabled and Immortality Begins at Twenty. This would logically provide people with immortality, but the average lifespan hasn't actually gone up that much, though; due to lifespan replacing money it's only the rich that really live forever. Will and Sylvia are as old as their appearances suggest, while Will's mother is double that, making her Older Than They Look. However, Sylvia's father, Phillipe, states during his birthday party that he is "25 for the 85th time", which means that he is 109 years old.
We Are the Night: Louise is more than two hundred years old, but appears to be at most forty. Charlotte is over a hundred, but looks around thirty. Nora is thirty seven, but still appears to be about twenty.
Nanny McPhee: The titular Nanny McPhee perpetually looks like a middle-aged woman, despite the first and second films being more than sixty years apart.
In Shandra: The Jungle Girl, Shandra looks like she is in her mod-20s, but is at least 90 years old, and there are strong hints that she is actually far, far older.
Vamps: Goody was born in the early 1800s, but looks no older than her thirties. Stacy turned at 20 in the early '90s and still looks the same two decades later. Other vampires like Cisserus and Tepes do look old, but don't show the centuries they actually are. Others appear to be even older.
In Frankenstein Island, Dr. Van Helsing does not look like a young man, but Doc is astounded to learn that he is over 200 years old.
In The Man Who Could Cheat Death, Gerrard estimates Bonnet's age as being around 35. He is actually 104, having extended his youth by means of his elixir.
Bit: Duke turns out to be somewhere in her sixties, as she was twenty-something during the early 1970s. Vlad is centuries old, while it's possibly implied he is meant to be Dracula. If so, he'd be around 580 years old.
Abby from Let Me In looks like a 12 year old girl, but she is a vampire. She was with her caretaker Thomas since childhood and he is a middle-aged man during the film. A deleted scene shows her being turned into a vampire by her uncle. This would give her an age of several centuries.
With a Kiss I Die: Juliet was born before 1303 sometime, but looks no older than thirty or so in the present, centuries later. The Greek vampires who turned her are even older, but don't look it either.
In The Witch Files, Jules is really more than 300 years old, and has been returning to Brunswick every 17 years to renew her immortality.