A prize winning Hereford cow who lost her farm to Alameda Slim after he stole all the cattle, except for her. Her owner moves her to "Patch Of Heaven".
- Big Beautiful Woman: Though not anthropomorphic, she is certainly treated like this. She even has a family friendly equivalent of Gag Boobs.
- Boisterous Bruiser: She's brash, and if her interactions with Mrs. Calloway are any indication, she's not one for respecting personal boundaries.
- Jerk with a Heart of Gold: Headstrong, aggressive, and a bit vain. You'd be hard pressed to find a more kind and loyal cow however.
- The Hecate Sisters: The Mother. Maggie is eccentric, plump, and in a rough way, motherly.
- Male Gaze: Shows off every inch of herself, and she has plenty of inches to show off.
- Sarcasm-Blind: In the scene where Maggie proposes her plan to save the farm to Mrs. Calloway, this happens:Maggie: Why don't we go nab that Alameda Slim and use the reward money to save the farm?Mrs. Calloway: Oh, that is a ''sensible'' idea.Maggie: I knew you'd love it!Mrs. Calloway: Don't they have sarcasm where you come from?
- Ironically, Maggie can completely understand Calloway's sarcasm in almost every other scene.
- Tomboy and Girly Girl: She's the tombull to Mrs. Calloway's girly cow. Maggie is boisterous and has a fighting spirit, while Mrs. Calloway is essentially a prim and proper old lady in cow form.
A sophisticated Holstein-Friesian cow living on "Patch of Heaven". She is best friends with Grace, and the unofficial leader of the animals.
- Berserk Button: Having her hat ruined turns her into a wild animal.
- Deadpan Snarker: She's prone to sarcastic remarks, such as when she says to Maggie "Oh, that is a sensible idea!" while meaning the exact opposite.
- The Hecate Sisters: The Matron. Mrs. Calloway is the most bitter and sarcastic of the cows, but also wise and kind.
- Interspecies Romance: With Junior the Buffalo at the end.
- Married Animals: Her name implies this, although it's entirely possible Pearl just named her that because.
- Nice Hat: Mrs. Calloway (even though she's a cow) wears a hat; and at a certain point in the movie when a buffalo is trying to work against the cows, he shouts to her "Nice Hat!".
- Tomboy and Girly Girl: She's the girly cow to Maggie's tombull. Mrs. Calloway is essentially a nice old lady in cow form, while Maggie is boisterous and has a fighting spirit.
- Younger Than They Look: Everything about her from her voice to her name suggests an older lady, but apparently, she's Grace's age.
A slightly air-headed but well-meaning Jersey cow living on "Patch of Heaven". She is best friends with Mrs. Calloway.
- Dreadful Musician: Grace sings waaaay off-key, much to Maggie's displeasure. Even the herder thinks she sounds terrible. Of course, she's tone-deaf, so it's justified.
- Dumb Blonde: She has such fair colouring, especially compared to the reddish Maggie and dark Mrs. Calloway, that she's the cow equivalent of a blonde. She's also somewhat the Ditz, with things going over her head, her being naive, etc.
- Hollywood Tone-Deaf: Pity anyone nearby when she tries to sing. It turns out to actually help when Alameda Slim comes along with his hypnotic yodel.
- The Hecate Sisters: The Maiden. Grace is ditzy, energetic, and incredibly optimistic.
- Immune to Mind Control: Because she's tone-deaf, she is the only cow not affected by Alameda Slim's Mind-Control Music, and she manages to snap her friends out so they can stop him.
- The Kirk: She's the one who has to tone down disputes between Maggie and Mrs. Calloway. And even among the other farm animals.
The sheriff's horse, who wants adventure and to prove himself by capturing bad guys, especially Alameda Slim. Rico is his idol.
- Breakout Character: Disney seemed to be making an assumption for this, as he was the one narrating the trailers and the star of the Gameboy Advance video game.
- HeelFace Turn: Subverted as he wasn't really evil himself but rather a selfish jerkass who only was unknowingly working for the Big Bad, but once Buck learns that Rico, the guy he idolizes and had been working with is a bad guy, he helps the cows escape after they are captured.
- Hot-Blooded: Grace refers to him as having anger management issues.
- It's All About Me: Wants to capture Slim for all the glory.
- Jerkass: Very egotistical and very obnoxious to the cows.
- Jerk with a Heart of Gold: Buck is initially annoying and selfish towards the cows, but after it's revealed that Rico's actually a mercenary who was working for Slim all along, he joins their team and helps them escape.
- Punny Name: His name is Buck, as in when a horse "bucks" someone.
- "Well Done, Son!" Guy: Even after Rico abandons him, Buck continues to search for the stolen cattle in order to impress him.
A "lucky" jackrabbit who lost his home, Echo Mine, when Slim took it over as his headquarters.
- Butt-Monkey: During the opening song, at least.
- Chekhov's Gunman: First appears in the opening song as a particularly unlucky rabbit and later appears to help the cow trio and leads them to where Slim is hiding.
- Jack-of-All-Trades: Jackrabbit of all trades: shaman, cook, chef, guide.
- Lucky Rabbit's Foot: Jack says people use to come all over to rub his lucky rabbit foot. The foot in question is now a peg-leg.
- Nice Guy: He's a pretty friendly fellow.
- Righteous Rabbit: Again, he's a nice rabbit.
The kind-hearted owner of Patch of Heaven.
- Berserk Button: The animals are the only family that Pearl has, so don't you dare intimate that she should sell any of them, even if the money could help to keep her farm afloat.
- Cool Old Lady: She's managed to run Patch of Heaven completely on her own for who knows how many years, she's won more agricultural awards than you could shake a stick at, and she told off the sheriff for suggesting that she auction off any of the animals.
- Heroic BSoD: When she receives the foreclosure notice, Pearl is forced to admit that while she's been able to squeak by before, she's "plumb out of ideas" this time. The cows' sudden disappearance only makes it worse.
- Nice Girl: Pearl is friendly and good-natured, and she loves the animals of Patch of Heaven as her family, showing genuine concern when Maggie, Grace, and Mrs. Calloway suddenly leave home.
A very large, yodeling cattle rustler. His plan is to steal all cattle from farms, buy the farms as they go bankrupt, and take control of the area.
- Acrofatic: He's big and he's agile, as shown when dodging Maggie's attacks at the beginning of his Villain Song.
- Arch-Enemy: To Maggie. He rendered her homeless at the beginning of the film and this serves as half the motivation to capture him (the other half being to save Patch of Heaven).
- Badass Boast: At the beginning of his Villain Song.
- Badass Longcoat: He wears a long trench coat during the opening verses of his Villain Song, and subsequently ditches it when he starts yodeling.
- Berserk Button: Much like Professor Ratigan does not like being called a "rat", don't call his yodeling "singing". He takes offense to that. Also, don't say no one likes yodeling or make fun of it in general.
- Big Bad: His capture is the object of the cows so they can save the farm.
- Card-Carrying Villain: Boasts about being mean and nasty in his Villain Song.They call me mean, boys
Depraved and nasty, too
And they ain't seen, boys
The cruelest thing I do
- Clark Kenting: As Yancy O'Del. His nephews can't catch on to the two being one and the same even as Slim puts on the disguise right in front of them. This is taken Up to Eleven when one of them dons the hat and spectacles and the other two freak out just as they did with Slim.
- Compelling Voice: When he yodels. It only works on cows, which is perfect for a cattle rustler.
- Evil Redhead: He's the Big Bad and has long, mullet-style red hair.
- Fat Bastard: A cattle rustler with an aim to drive every ranch in the West out of business and XXXXL size pantaloons.
- Freudian Excuse: He used to work for other ranchers but felt he was looked down upon. He, therefore, turned to cattle rustling to get revenge on them.
- Insistent Terminology: Is insistent that he yodels instead of singing.Alameda Slim: Songbirds sing. Saloon gals sing. Little bitty snot-nosed children sing. I yodel! And yodeling IS AN ART!
- Ironic Name: Alameda Slim... isn't.
- Large and in Charge: He is indeed very large, and in charge.
- Large Ham: Like many other Disney villains. Randy Quaid seems to be one of the few good things critics and fans can find in the film.
- Mind-Control Music: Well, mind-control yodeling, anyway. Beats traditional wrangling any day, as far as he's concerned.
- The Music Meister: Slim's hypnotic yodeling makes the cows dance along to his Villain Song/Disney Acid Sequence "Yodel-Adle-Eedle-Idle-Oo."
- Pragmatic Villainy: Alameda Slim's plan is this overall. He is out for revenge not by killing people, but instead by driving the people he worked for out of business. He uses his unnatural yodeling to steal a farm's main source of income, cattle, and sells the cattle on the black market. He then waits for the farms to go up for auction and uses the money from the cattle he sold to buy the foreclosed farmland.
- The Rustler: Said to be able to steal five thousand cattle in one night. Mass Hypnosis through yodeling definitely helps in that regard.
- Stout Strength: Shown to be able to smash a mine cart with his bare hands and get all three cows in a headlock.
- Surrounded by Idiots: How he views his three nephews who work for him.
- Wicked Cultured: Slim has an impressive repertoire of tunes to yodel, like the "William Tell Overture", "Yankee Doodle", "Ode to Joy", "Ride of the Valkyries", and the "1812 Overture".
The Willie Brothers
Slim's dim-witted nephews who assist him in his cattle rustling.
- Beware the Silly Ones: All three are dim-witted and bumbling, but prove to be effective enough while actually cattle-rustling; they subdue the men guarding the cattle before Slim hypnotizes the cattle and manage to count to 4,997 on their first try (the only reason they didn't count 5,000 cattle as they should have was because the three main cows were unaccounted for).
- Blinding Bangs: All of them (and their horses) have these, and there is one exactly frame where one of their eyes can be seen.
- Bumbling Sidekick: Of all the sidekicks in the West, Slim had to pick these idiots.
- Color-Coded for Your Convenience: Each has a differently colored bandana and hat band; Phil has red, Gill has green and Bill has blue.
- Dumb Blonde: Male examples. All three are blond, ditzy guys who are slow on the uptake.
- Even Evil Has Standards: When Slim tells them he plans on buying out "Patch of Heaven", his nephews mention its only a small dirt farm and question if it is worth it. To Slim, who wants to own everything, it is.
- Expy: They are essentially based on Ward Kimball's Pecos Bill from Melody Time.
- Nice Hat: All three of them wear bowlers.
- Siblings in Crime: A set of triplets that assist their Uncle Slim in cattle-rustling.
- This Is My Chair: Gil just happens to sit in the exact same spot in front of Slims map that blocks off Patch of Heaven from being seen by Slim."This is my comfy place." [Slim starts throttling him]
- What Happened to the Mouse?: They (along with Wesley and Rico) are left unconscious inside a train car.
A bounty hunter who is hired to capture Alameda Slim. He is Buck's idol. He is really partnered with Slim.
- The Ace: He is known to be one of the best bounty hunters in the area.
- Bitch in Sheep's Clothing: Rico appears to be on the side of the law but is working with Alameda Slim.
- Broken Pedestal: To Buck, once he learns his true colors.
- Dark Is Not Evil: He has a gravely voice and dresses in dark clothing but is after Slim as well. heavily averted when he is revealed to be working with Slim the entire time
- Evil Pays Better: Rico is a respected bounty hunter but is also partnered with Slim to earn more money.
- The Mole: Rico first appears hunting Alameda Slim but is revealed to have been working with him. Slim mentions Rico has helped thrown off his trail to keep him hidden.
A diminutive man who pays Slim for the cattle he steals. Slim calls him Mr. Weasley.