- Voiced by: June Foray
- Damsel in Distress
- Expy: To the extent that she was actually called "Jane" in the unaired pilot. Her name was changed to avoid trouble from the Edgar Rice Burroughs estate.
- Housewife: While George goes off on his adventures, Ursula typically remains behind in the tree house. Often, she is shown doing domestic chores such as laundry and knitting. If she does get involved in an adventure, it's probably because the villains kidnapped her.
- Played by: Brendan Fraser (first film); Chris Showerman (sequel)
The king of the jungle, who as a baby was lost in the African wilderness following a plane crash.
- Eloquent in My Native Tongue: In English, he uses Hulk Speak. In ape, he's positively Shakespearean.
- Friend to All Living Things: George will befriend every person and animal he meets, and help every person or animal in need, unless they're threatening the innocent. (Like the lion that tries to eat Ursula, Those Two Bad Guys that try to kidnap Ape, or Lyle trying to kidnap Ursula...)
- Good Samaritan: While climbing the San Francisco bridge, George catches sight of a skydiver whose parachute got caught in the bridge. George wastes no time swinging down to him, untangling him, and helping him climb down to safety.
- Hunk: Frequently commented on and gushed over by Ursula's female socialite friends.
- In Touch with His Feminine Side: Not afraid to share his emotions, wear tons of hibiscus flowers, or run around San Francisco in a dress.
- Idiot Hero: Played with. He's Book Dumb and uses Hulk Speak (in English), but he's not a complete idiot. He's emotionally intelligent, gifted at calibrating where and how best to swing, and quick to pick up on modern San Francisco technology. As the women admiring him frolicking with horses point out, George isn't dumb, he just has a different kind of intelligence.
- Nice Guy: Frequently described by the narrator as "Defender of the innocent, protector of the weak, and all-around good guy, George of the Jungle."
- Tarzan Boy: Ursula even calls him a "Tarzan-wannabe".
A San Francisco heiress who travels to Africa on a safari and falls in love with George there.
- Character Development: At the start of the movie she's very sweet but too timid to tell off her socialite peers, like Lyle or her parents. Thanks to spending time with George, she eventually musters the "inner juju" to tell her parents she doesn't want to marry Lyle anymore, and even try to tell Lyle off herself.
- Establishing Character Moment: The narrator ominously describes to the audience how intruders are trespassing in George's kingdom. Description Cut to Ursula sweetly greeting the camera as she documents her trek through Africa, gushing over the animals and how sweet her native tour guides are.
- Extreme Doormat: Before her Character Development, she's too timid to tell Lyle off for being a controlling jerk, or later to tell her parents she doesn't want to marry Lyle anymore.
- Friend to All Living Things: Like George, she's very nice to every person and animal she meets.
- Going Native: She abandons her high-class life to live in the jungle with George.
- Hair of Gold, Heart of Gold: Has perfectly permed blonde hair and a sweet disposition to match.
- Housewife: Settles into this role in the sequel, not unlike her role in the original cartoon.
- Nice Girl: Only matched by George in how nice she is.
- Spoiled Sweet: Is a rich heiress, but very kind to everyone.
- Wakeup Makeup: Lampshaded by the narrator, when she wakes up fresh-faced with a perfect perm.
- Voiced by: John Cleese
- Deadpan Snarker: The snarkiest in the movie, after the Lemony Narrator.
- Irony: Ape the gorilla has the intellectual personality of a refined human, while George (his adopted human brother) has the more primal personality of a gorilla.
- Act 3 is also all about saving Ape from being sent into Vegas show business against his will. The credits reveal that Ape went into Vegas show business anyway, but on his terms.
- Sarcastic Confession: Inverted. Ape tells his kidnappers truthfully that the trail they're on is a fake and taking them right back to the tree house. But due to his sarcastic delivery, they assume he's lying and push forward. Naturally, they end up right back at the tree house.Ape: Well, I tried, but you fellows are just too smart for me.
- Surrounded by Idiots: Often feels this way, both when George is and isn't around.
- Played by Thomas Haden Church
Ursula's snobby and useless Disposable Fiancé who serves as the movie's main villain.
- Chekhov's Gun: His novelty lighter that looks like a pistol. It gets swapped with a real pistol after Lyle asked one of the native tour guides to clean it, and accidentally shoots George as a result.
- Control Freak: Won't let Ursula have a moment to herself, even for her personal trip to Africa.
- Dirty Coward: Ditches Ursula when they run into a lion (but trips on a root and gets knocked out instead). Later, he also declares he wants to fight the "white ape" who kidnapped his girlfriend, but then pauses and asks the guy with the binoculars if it seems like Lyle could take him before proceeding.
- Disposable Fiancé: Of the "Jerkass" variety.
- Moral Event Horizon: In-universe, the other characters all thought Lyle was just kind of a rich snobby jerk until he shot George. Said shooting landed him straight into "irredeemable criminal who belongs in prison" territory for them.
- No Hero to His Valet: In San Francisco, Lyle is beloved by Ursula's rich socialite socialite community. In Africa, Lyle is loathed by the native tour guides for the rich, snobby jerkass he is.
- Politically Incorrect Villain: Downplayed since it's a family film. Still, he's pretty condescending and controlling of Ursula compared to George being respectful of her wishes, and treats the native guides like dumb savages.
- Rich in Dollars, Poor in Sense: So. Very. Much. Let's see; he assumes the rope bridge they're on is safe just because the kitschy touristy one he was on in Maui was safe. He tries to impress the native tour guides with his "magic pictures" only for them to reveal they like another camera model better; and his lens is dirty. He also carries around a novelty lighter that resembles a pistol and waves it around recklessly (which causes him to accidentally shoot George.)
- Played by: Holland Taylor (first film); Christina Pickles (sequel)
Ursula's snobby, meddling mother who still wants Ursula to marry Lyle.
- Does This Remind You of Anything?: Her disapproval of Ursula's relationship with George is very reminiscent of parents disapproving of an interracial relationship. She even uses the old "stripes and spots" analogy.
- Lady Drunk: Is often seen with a drink in hand.
- My Beloved Smother: Very meddlesome in Ursula's life.
- Maligned Mixed Marriage: She thoroughly disapproves of her heiress daughter's relationship with an "ape man" from the jungle.
- Nice Job Fixing It, Villain!: After George leaves, Beatrice tries to assure Ursula the type of "love" she feels for George is fleeting. Unfortunately, using that word gives Ursula the Love Epiphany she needs to go after him.
- Reformed, but Not Tamed: By Ursula's wedding, Beatrice is more or less resigned to her relationship with George, though Beatrice still complains about it being filled with monkeys.
- Voiced by Lee Tockar
The king of the jungle, only scrawnier and younger, yet still strong.
- Catchphrase: His Tarzan yell.
- Voiced by Cory Doran
The king of the jungle
- Apparently Human Merfolk: George becomes one of these in "Wet Behind the Ears", where he gets some gills in order to save the lake.
- Battle of the Sexes: Him vs. Ursula
- The Rival: 2 of them: Steve of the Jungle and, more obviously, Ursula.
- Voiced by Rob Tinker
- Voiced by Linda Ballentyne
The Self-proclaimed science genius.
- Adaptational Name Change: Her name was Ursula in the 2007 cartoon.
- Damsel in Distress: Since Ursula has become a badass
- Villain Protagonist: In "Strong as He Can Tree", "Valley of the Magnolias", and "For Science"
- Voiced by Bridget Wareham
The wild girl who wanders in the Jungle
- Absentee Actor: She's absent for most episodes
- Adaptational Name Change: Her name was Magnolia in the 2007 cartoon.
- Adaptational Personality Change: From the valley girl-speaking jungle girl in the 2007 cartoon.
- Badass in Distress: On "Ursula Solution", she was running in fear after Tiger stole her feral rage and also gets chased by Feral Tiger.
- Berserk Button: Being touched
- Battle of the Sexes: Her vs George
- Courtroom Antics: On "Breaking Ape", when there seems to be no prosecutor for George's trial, Ursula comes to court and reveals that she's the prosecutor, told the judge that George is guilty, and forces the judge to send George (and also Ape) to prison.
- Freudian Excuse: According to Evil!George in "Heart of Gold" she wants to be an animal because she's terrible at being a person. Her crying in response seems to confirm this.
- Hulk Speak: She doesn't speak properly, and also kinda sounds like him.Ursula: "Ursula no like talking plant!"
- Jerkass: Mostly to the heroes
- Mysterious Past: There's nothing known about her past, therefore making her an Orphaned Hero like George.
- The Rival: The obvious one is George.
- She's Got Legs: Uses them for picking her nose and scratching her face.
- You Don't Look Like You: On this version, she doesn't wear make up.