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- Adaptational Nice Guy: Not the arrogant jerk he's depicted in canon or most adaptations.
- Adorkable: He certainly has his moments, particularly around the Irregulars.
- Back from the Dead
- Badass Longcoat
- Catchphrase: "Eyes and brains!"
- Character Development: He goes from rejecting Watson to embracing him wholeheartedly in the span of two episodes. Plus, his relationship with Beth develops subtly throughout the series.
- The Chessmaster
- Deadpan Snarker/Gentleman Snarker
- Fat and Skinny: The skinny to Watson's chunky physique.
- A Father to His Irregulars
- Fish out of Temporal Water: Not for long, though.
- Genre Savvy: To the point of reading up on Dracula when dealing with a vampire.
- The Hero: Becomes an action hero with the episodes following him on his investigations even when he's working alone.
- Incredibly Lame Pun: Has an unfortunate propensity for these.
- Insufferable Genius: He's much nicer than he was in his youth, but he still has his moments.
- It's Personal: If it's Watson, absolutely. Lestrade quickly reaches this status, as well.
- Jumped at the Call: Threw himself right into his first case.
- The Leader
- Master of Disguise/Impersonation Gambit: So much so that he can fake different American accents. Of course, elastomasks also help, especially as many of his disguises are real people.
- Not So Stoic
- Older Than They Look
- The Spock
- Took a Level in Kindness: Holmes is noticeably softer in the show, when compared to his younger self in the Sherlockian Canon.
Inspector Beth Lestrade
- Action Girl: She's quite a formidable woman, not above kicking in doors or taking down suspects herself, and handy with an ionizer besides.
- Badass in Distress: In spite of her being possibly the more physically formidable and definitely the more aggressive of the two, she does occasionally end up needing to be saved by Holmes.
- Brainwashed and Crazy: Done once with a puzzle box laced with Nanomachines.
- Cowboy Cop: Naturally, as a foil to Grayson.
- Destructive Saviour: Grayson reads a laundry List of Transgressions inflicted in the process of catching Fenwick in the first episode, and it's implied it isn't the first time her enthusiasm for catching criminals has come at a price.
- Drives Like Crazy: Demonstrated a fair number of times over the course of the show, enough to be gently snarked on the topic by Holmes.
- Hot-Blooded: Lestrade is quite unrestrained in her emotions, as a contrast to the calm and genteel Holmes.
- Inspector Lestrade: Literally in name, and like her ancestor she starts off making some hot-headed presumptions in cases as to who is guilty of what. That tendency smooths out over the course of the show.
- The Knights Who Say "Squee!": She is both an officer of the law and a devoted admirer of Holmes' talent for outwitting criminals. Enough to suggest revivification to a highly skeptical Chief Grayson.
- Legacy Character: Of a sort. She is directly related to the original Inspector Lestrade and assumes a similar role to the first as Holmes' point of contact with Scotland Yard and actual figure of authority in a case. Her relationship is much less vitriolic than the original relationship between Holmes and Lestrade.
- The Not-Love Interest: She's the only female in the main cast and is working alongside and butting heads with Holmes, but the two share no romantic interest.
- Trigger Happy: Lestrade draws her ionizer at the earliest opportunity in many cases. Fortunately she tends to holds her fire if innocents are in the way. When she only has to worry about a suspect, she doesn't hesitate to fire.
- Turn in Your Badge: Threatens to do this to Grayson and take her story of a revivified Sherlock Holmes to the news to make New Scotland Yard look like they were shirking their duty to help the public by ignoring potential assistance.
- Screw the Rules, I'm Doing What's Right!: Her stance on New Scotland Yard's limitations, and Grayson's inflexibility especially.
- Adorkable: Something he picked up from studying the original Watson.
- Badass Moustache: As well as large muttonchops.
- Badass Longcoat: He is commonly seen wearing a large greatcoat that covers all but his feet and forearms.
- The Big Guy: Largest of the three protagonists and the strongest due to being a robot.
- Do-Anything Robot: He seems to always have just the right scanning function or tool for the job at hand.
- Everything Sensor
- Fat and Skinny: The fat to Holmes' skinny. Explicitly stated in-universe by Holmes as sharing a somewhat potbellied appearance with his namesake, the original John Watson.
- Nice Hat: A bowler hat.
- Ridiculously Human Robot: In at least one instance, he coughs due to a cloud of smoke. The robot below the elasto-mask has neither mouth nor lungs.
- Robot Buddy: Naturally.
- Robot Me: In an interesting variation, the robot consciously chose to model himself on Holmes's old friend.
- Side Kick
- Super Strength: Comes with being a robot.
- The Watson: Goes back and forth on this; occasionally he asks questions to give Holmes a chance to reveal what he has discovered, but will also bounce his own deductions and inferences off Holmes.
- What Measure Is a Non-Human?: "The Fall And Rise of Sherlock Holmes" and "The Crime Machine" let Holmes explore his relationship with Watson. "The Five Orange Pips" revolves around this trope.
Chief Inspector Grayson
- Arbitrary Skepticism: No matter how many times he sees Sherlock Holmes crack a case, pick up on facts no one else noticed, and solve crimes, he constantly refuses to believe that Holmes is any help at all.
- By-the-Book Cop/Lawful Stupid: Grayson straddles the line of this due to his unwillingness to deviate from police procedure for even a moment.
- Da Chief: Of course.
- Fat Idiot: His portrayal, albeit probably not intentionally so by the writers. Comes across as this, as the result of his combination of husky build (with an obvious paunch) and his incompetence in the course of fighting crime, including once being Moriarty's Unwitting Pawn to provide an alibi for a crime. He was also introduced getting an elasto-mask of an old woman stuck on his head.
- Large and in Charge: Physically a large man, and commands New Scotland Yard.
- Obstructive Bureaucrat: His adherence to the rules and declarations of limits hinder investigations of cases more than helps them.
- Police are Useless: He may mean well in his lawfulness, but is chronically unhelpful.
- Turn in Your Badge: Does this at least once to Lestrade. It doesn't take.
- Bald, Black Leader Guy: Of the Irregulars.
- Hidden Depths/Smarter Than You Look: In spite of his somewhat thuggish mode of dress, he winds up demonstrating enough talent and astuteness at their first meeting to impress Holmes.
- I Coulda Been a Contender!: Once a talented football (soccer) player until an injury put an end to his sport ambitions. He has since taken up boxing.
- Photographic Memory: According to a poster of the characters.
- The Southpaw: Holmes pegs him as a lefty in their first meeting, which can be beneficial for an amateur boxer.
- Sleeves Are for Wimps: Always seen in a sleeveless duster.
- The Yardies: Black and an implied juvenile delinquent, albeit a Lighter and Softer version to the point of general harmlessness.
- Black Market: Implied to deal with this, as she's a con artist and a fence.
- Con Girl: Specifically, Little Miss Con Artist.
- Troubling Unchildlike Behavior: There is something quite amiss in a twelve year old girl who knows where to sell varieties stolen goods of high value.
- Younger Than They Look: According to the character poster, she's twelve!
- Child Prodigy: According to the character poster, a computer and mathematics genius.
- Cool Mask: Always seen with a blue mask over his nose and mouth.
- Genius Cripple: Math wizard, brilliant hacker, always seen in a hovering wheelchair analogue.
- Goggles Do Nothing
- Hollywood Cyborg: He's never seen out of his hoverchair, and we've never even seen him below the waist. The size of his chair versus Tennyson's upper body implies that he may not have legs.
- Intelligible Unintelligible: Speaks in beeps, warbles, and musical tones, but is understood by Holmes and the Irregulars.
- Playful Hacker: Can break into computers easily, but is never malicious about it.
- Super Wheelchair: It hovers and is loaded with computer tech for hacking purposes.
- Younger Than They Look: The youngest of the Irregulars, at eleven.
Professor James Moriarty
- Affably Evil: Can be a gentleman and quite casually so. It's almost a little creepy, as befits the original character.
- In his initial appearance, this leads to a I Kiss Your Hand moment with Beth, who is not amused.
- Badass Baritone/Evil Sounds Deep
- Badass Longcoat: Favors a cobalt-blue trenchcoat while doing his own dirty work.
- Big Bad/The Man Behind the Man: There are very few episodes that don't feature Moriarty as the mastermind behind the scenes.
- The Chessmaster
- Deadpan Snarker/Tall, Dark, and Snarky
- Diabolical Mastermind/Evil Genius
- Even Evil Has Standards: According to Moriarty in "Five Orange Pips," he doesn't want people getting hurt as he takes over the world. To be fair, he generally sticks to this rule, with the exception of "Baskerville" early on in the game.
- Evil Genius: Positively brilliant, considered Holmes' equal, and as dedicated to committing crimes as Holmes is to solving them.
- Evil Laugh
- Hidden Villain: For the first couple of episodes.
- Hoist by His Own Petard: The way he tends to get his due.
- Knight of Cerebus
- Large Ham: "But of course!"
- Muscles Are Meaningless: Moriartys impressive build never stops the sleeker Holmes from mopping the floor with him.
- Politically Incorrect Villain
- Skunk Stripe: Has a huge one in his hair.
- The Starscream: He accomplishes this offscreen. Originally cloned to be Fenwick's accomplice, he upstages Fenwick as the true criminal mastermind.
- Took a Level in Badass: This is a man who enjoys getting his hands dirty and enjoys sparring verbally and physically with Holmes, quite unlike the reclusive intellectual of the Canon.
- It does make sense, though, as this Moriarty is a clone of the original created by Fenwick, who was supposed to serve as his Dragon. Presumably, there was some manipulation involved to make him more suited to the task.
- We Will Meet Again
Dr. Martin Fenwick
- Demoted to Dragon: After his clone of Moriarty upstages him
- The Dragon: To Moriarty.
- French Jerk: Stated to be a native of Neo-Paris.
- Gonk: He has the most blatantly ugly mug in the series, almost to the point of seeming inhuman—and this is a series where both a massive cat-man and a snakeman have shown up in episodes!
- Our Ghouls Are Creepier: His visual effect due to his pale skin, mismatched eyes, upturned nose, bald head, and massive underbite.
- Just a Stupid Accent: Speaks grammatically correct English with a terrible French accent. Holmes once catches him because of his inability to hide his distinctive accent.
- Poirot Speak: Occasionally peppers his speech with random stereotypical French phrases.
- Sssssnake Talk: While he lacks the reptile motif, his accent and inability to enunciate leads to this.