In scientific, medical, or high-security settings, X-Rays are used and may sometimes show funny and/or unusual things on the subject they are used on. In other scenarios, X-Ray Sparks can serve the same purpose.
- Exaggeratedly large or small brains in the head, representing how smart or dumb a character is.
- Sometimes a small object may be substituted for a brain to represent a character's stupidity.
- Unusual objects in the stomach, showing that a character is an Extreme Omnivore.
- Objects up the asshole... or in other funny places.
- Objects that probably shouldn't be shown on X-Ray, probably due to being an important element of a character or Critical Research Failure.
- Very dangerous objects, usually at Checkpoint Charlie and other high-security settings.
- Nothing at all, including lack of skeleton, to show something is unusual about a humanoid character.
Compare with Funny Terrain Cross Section. In medical settings, ultrasound may be used as an alternative.
- In Episode 29 of Bobobo-bo Bo-bobo, Rice goes to a hospital in the middle of the battle, with Bo-bobo being his doctor. Bo-bobo shows Rice his x-ray, but instead of a human skeleton, it's a strange, cartoonish imp-like creature with a giant head, pointy ears, and a tail. Bo-bobo claims he found nothing wrong, but Rice starts screaming...because the x-ray shows some kind of growth on his ear.
- In Osmosis Jones, Ozzy goes through an X-ray machine while entering the stomach to meet Drix. The X-ray shows his gun, and he gets a pass by showing his badge. The guy behind him, however, is a tough-looking sailor who is revealed to be wearing women's underwear.
- An episode of Married... with Children has Jefferson in the hospital telling the story of how he got there by wanting to get a gift for his wife, Marcy, culminating in his getting a tattoo that misspelled Marcy's name as Marty. Marcy's reaction is revealed at the episode's end when an X-ray of his rear end reveals the outline of a boot.
- In the episode "My ABC's", J.D. fantasizes about the Muppet cast of Sesame Street joining the staff at the hospital. During his fantasy, he must inform a patient named Ex Ray (also a Muppet) that Ex Ray's X-rays show there is a human hand inside his head. According to Ex Ray, "This explains so many things."
- Another episode has the X-ray of a man who had a light bulb up his ass.
Dr. Cox: Either this guy's got a lightbulb shoved up his ass or his colon has a really good idea.
- In one of Kermit's lectures on Sesame Street, Herry Monster stands behind an X-ray machine which displays high-resolution color animations. After Kermit sets the machine to show Herry's muscles, Herry steps away from the machine to watch the show. A puzzled Kermit gets behind the X-ray machine, and Herry turns the tables on him.
- Plants vs. Zombies 2: It's About Time: the X-Ray Sparks for the Gargantuar Pirate reveal a whole dinosaur skeleton inside its body.
- Trauma Team: as the diagnostics involve examining various scans, you can spot interesting anomalies to get achievements. One of the easier ones being the EKG machine being on the fritz.
- In Persona 4, Teddie has no skeleton in his human body, which dumbfounded many doctors.
- BlazBlue: The X-Ray Sparks of some characters show some funny tidbits:
- Taokaka apparently stuffs a fish under her long sleeves. Kokonoe stuffs sweets in the same manner.
- Hakumen doesn't show any skeleton (being an Animated Armor), instead he just shows some odd Tron Lines.
- Terumi briefly shows his ghostly features instead of his skeleton.
- Relius apparently has his arm and a leg replaced by prostheses.
- Skullgirls: Valentine's Steam Trading Card shows some X-Rays of Valentine's body, one of showing her cracked heart, Filia and Samson's head, and Peacock's hand.
- Batman: Arkham Series:
- Batman: Arkham Asylum: Using Detective Mode on Clayface reveals that he has no skeleton.
- Batman: Arkham City: Using Detective Mode during the Joker fight reveals that Joker has no skeleton. It later turns out Clayface was impersonating Joker, meaning Clayface's cameo in the previous game was foreshadowing.
- Phoenix Wright: Ace Attorney Spirit of Justice: After Pierce Nichody goes into his One-Winged Angel form by dressing more like a surgeon, the FIXR-UPR mech that he modified produces X-rays of Phoenix Wright and Miles Edgeworth, which contain their Anime Hair and Classy Cravat respectively.
- In the original Super Smash Bros., when Samus gets hit by an electric attack, instead of a skeleton, the X-Ray Sparks reveal a silhouette of her body.
- Q and Twelve in Street Fighter III: 3rd Strike don't show their skeletons when shocked. Q instead shows his muscular system, with his head still looking mechanical, further adding to speculation as to whether or not he's human. Twelve doesn't show anything at all, as he's made of a malleable substance that allows him to shapeshift. Necro is also shown to have an unusual skeleton, with his bones being broken into small segments, explaining how his stretching powers work.
- Necalli in Street Fighter V is shown to have no skeleton at all when shocked. Just an empty, black void.
- Team Fortress 2: An X-ray showing a ribcage with a bomb inside is seen in "Meet the Medic", along with the Medic displaying it in his "Results Are In" taunt. Very likely it is an X-ray of the Heavy's body.
- The Homestar Runner Halloween toon "Halloween Fairstival" has the King of Town show Strong Bad and The Cheat an x-ray of his heart, which resembles a honey-glazed ham. Strong Bad says it isn't scary, but it is promising that this probably means the King of Town will die soon.
- In one part of Sparklecare, Dr. Memento takes an X-ray of an anthropomorphic banana. It has what looks like a normal banana inside it.
- Jones, a mysterious immortal denizen of the court in Gunnerkrigg Court, seems to be entirely impervious to any form of harm, including radiation. Her X-rays resemble silhouette portraits more than they do medical scans.
- Cheer Up Emo Kid combines this with Ambulance Cut in this strip.
- On the opening sequence for the Pinky and the Brain segments on Animaniacs, the two title characters walk by an X-ray machine. Brain has gears inside his head, while Pinky has a peanut.
- Jof the cat from the What A Cartoon! Show short "Help!" by Bruno Bozzetto sees a doctor about a sore finger. The peculiar doctor wants an X-ray and indicates a large container of disclosing fluid for Jof to drink. Jof downs all the fluid — which doubles his body mass — then stands behind the x-ray screen. At first, Jof's skeleton appears normal, but Jof develops hiccups that rearrange his bones into a spider-like pattern, then a Jolly Roger pattern, and lastly a hammer and sickle symbol. The last one makes the doctor forsake his oath to "above all, do no harm." Jof runs for his life.
- The Simpsons: an X-ray showed that Homer had a crayon stuck in his brain, dating back to when he shoved it up his nose back in school. It was lampshaded that it was never seen before because Dr. Hibbert always had the crayon blocked by his thumb on other X-rays.
- In "Deep Space Homer," the power plant security X-ray uncovers various unusual things: several employees packing heat, two kids in a Totem Pole Trench, and some sort of ape-like hominid. note
- During the lead-up to the Affectionate Parody of Willy Wonka & the Chocolate Factory, the Professor is using his "F-Ray" on Bender to figure out why he's not feeling well. While the F-Ray is showing the inside of Bender's head, a computer chip with the number 6502 on it can be seen briefly. note
- Once when Hermes got by an X-ray beam in "The-Duh-Vinci-Code", we see a disproportionately small skeleton inside his stout body, so he remarks:
I'm not big-boned, I'm just fat!
- My Little Pony: Friendship Is Magic:
- Family Guy: In an infamous pre-9/11 deleted scene where Stewie distracts airport security by singing, an X-Ray reveals a tiny AK-47, grenades, and other weapons inside Stewie's baby backpack. He is followed immediately by Osama bin Laden doing a similar routine.
- A similar scene appears in Season 3 of BoJack Horseman, as Todd accidentally falls asleep in BoJack's suitcase, but passes through airport security without comment, as does the suitcase full of firearms immediately after him. The one with a shampoo bottle, on the other hand...
- SpongeBob SquarePants: In "Pressure", as Sandy is holding her breath underwater, an X-ray view of her chest is shown with her lungs collapsing, and one breaking off.
- In the Barney Bear cartoon "Wee-Willie Wildcat", after Barney tries to kick a football with a rock inside it, a cross-section of his body shows his entire skeleton shattering to pieces.