There is some Truth in Television to this: roosters usually have a high testosterone level, which makes them highly aggressive and territorial, attacking other roosters and even humans, which is why they are often used in cockfighting. They also often peck at subordinate chickens to ensure their dominance.
- In Red Soul, at the height of the Red Scare and Cold War-era nuclear paranoia, the Blacksad universe's equivalent of Senator Joe McCarthy is a a beady-eyed cockerel with a huge comb named Senator Gallonote .
- In the Asterix comic book Asterix and the Class Act, Chanticleerix, the village rooster of the gauls gets A Day in the Limelight. He acts far more cocky than the other village chickens, though its mostly since he sees it as his duty to defend them from the Roman Imperial Eagle.
Chanticleerix: Shut your big beak or you'll feel mine in short pecking order!
- In Chicken Run, there are two main rooster characters. Fowler, the elderly resident rooster fits the stereotype better, being a Grumpy Old Man and a military veteran. Rocky, the rooster who lands on the farm from the outside, is a bit of a braggart but not particularly mean or arrogant. He does fit the other half of the stereotype as a shameless flirt always wanting to bask in the hens' attention.
- Gender Flipped in Robin Hood: Lady Kluck, a hen, is a boisterous, loud-mouthed character, whereas Allan-A-Dale, a rooster, is a friendly minstrel who narrates the story.
- Moana: During production, Hei-Hei the rooster was originally designed as cranky and obsessed with maintaining good behavior from Moana. However, test viewings found him to be unlikable that way, so his personality was changed in the final film from "bossy jerk" to "really stupid friend".
- Master Chicken in Kung Fu Panda 3 is a brave and powerful kung-fu warrior. Although his plumage is not particularly rooster-like (i.e. his tail feathers are relatively short), he's referred to by male pronouns.
- Buck Cluck, the title character's father in Chicken Little, is a bit of a Jerk Jock who is very disappointed that his son is an unpopular nerd.
- Ogden Nash wrote "The Rooster" as part of a collection of short poems connected to Camille Saint-Saens's "Carnival Of The Animals":
The rooster is a roistering hoodlum,
Its battlecry is Cockadoodlum.
Hands in his pockets, cap over eye,
He whistles at pullets passing by.
- According the Eastern Zodiac, those who are born in the year of the Rooster have traits such as being loyal, observant, honest to the point of being blunt, arrogant, miserly, and fanatical at their worst.
- The national animal of France is the "Gallic rooster", symbolizing their boisterousness and pompousness.
- The Sarimanok of Filipino mythology is essentially a rooster mixed with The Phoenix—a mystical bird associated with fire and known to be of all colors of the rainbow.
- Burn Rooster from Mega Man X8 is a rather aggressive and hot-tempered rooster maverick. In his defence, he's somewhat justifiably angry towards the Maverick Hunters for labeling reploids as Maverick (rightfully or not) and subsequently retiring them.
- Popla Cocapetri of Mega Man Zero 4 is an arrogant, high-tempered rooster Reploid who's proud of his hacking abilities and is a tricky opponent in battle due to his nonstandard tactics.
- Pokémon: The Torchic line, one of the starting Pokemon in Pokémon Ruby and Sapphire, is based on chickens, and is dual-typed Fire/Fighting, both associated with aggressiveness and strong attacks. The middle evolution Combusken has a permanent Death Glare.
- Hotline Miami 2: Wrong Number has Richard, a mysterious figure who wears a rooster mask. Richard is enigmatic and always appears to characters who will die in the next chapter of their story. It's never explained what Richard is and how he always appears.
- Panchito Pistoles, the Mexican rooster that debuted in The Three Caballeros and has appeared in various Disney media, is generally a Nice Guy, but has a sanguine, energetic personality, and at least in his early appearances he was quite the Gun Nut.
- Steelbeak from Darkwing Duck is a secret agent working for the villainous organization F.O.W.L.; he's an evil mastermind with a smug, arrogant attitude, who is usually cowardly, but can weaponize his steel beak when necessary.
- Scratch from Adventures of Sonic the Hedgehog is a robotic example. He is a robot rooster who is a member of the Super Special Sonic Search and Smash Squad, and always tries to ensure that he is Dr. Robotnik's favorite lackey, often getting into arguments with his non-identical twin brother, Grounder. He is also known for his signature laugh, "Bwahahaha!", and is the twelfth and semi-final opponent in the Dr. Robotnik's Mean Bean Machine video game, wherein the Sega Genesis version of the game, he makes a lot of chicken jokes to taunt the player.
- Foghorn Leghorn of Looney Tunes fame is quite a mean-spirited prankster, and also has a loud-mouthed, arrogant attitude.
- "Each Dawn I Crow," a Looney Tunes film from 1949, stars John Rooster, a cock-o'-the-walk who gets influenced by the cartoon's narrator that maybe his goose is cooked.
- Fowlmouth from Tiny Toon Adventures is Foghorn Leghorn's Junior Counterpart. In his debut appearance, "To Bleep or Not to Bleep" (part of "Test Stressed"), he has an obvious crush on Shirley and would love to ask her to a dance, but his swearing habit drives her away, and he enlists Buster's help to cure him of it. When Buster discovers that Fowlmouth refuses to swear in front of little children, he uses that to his advantage, and brings the three babies they passed by in the sandbox, so he can pull them out when it seems like Fowlmouth is about to swear. His plan works, as Fowlmouth doesn't even swear when Shirley tells him that she promised Plucky she'd go to the dance with him. In the Direct-to-Video movie, "How I Spent My Vacation", while Fowlmouth is a lot less profane, he's still very pushy and rude, particularly in movie theaters, proven when he and Shirley go see Skunknophobia, a movie that Fowlmouth has seen 100 times before, and will not shut up through. Eventually, it's not just the movie-goers who are sick of Fowlmouth's constant talking, but the actors of Skunknophobia as well when the movie-goers toss Fowlmouth into the movie itself.
- Ernie the Giant Chicken is a recurring antagonist in Family Guy. He's a human-sized yellow rooster who often gets into violent brawls with Peter Griffin for no apparent reason.
- The Silly Symphonies short "Cock O'The Walk" is about a fight between a burly black rooster and a scrawnier brown one over a pretty hen. The black rooster fits this trope to a T as a showy, bullying, macho, cigar-chomping boxing champion; the brown rooster is more of an underdog with a hopeless romantic personality, but he has a fierce temper, as he's the one initiating the fight, and becomes an invincible fighting machine after getting kissed by the hen.
- In the Terrytoons cartoon "The Orphan Egg", Dinky Duck attempts to smuggle the titular eggs under a hen, but the hen's husband, a rooster, angrily picks up the egg and throws it out from the chicken coop. When the egg hatches, the rooster also attacks the chick with a pitchfork; then, when a fox attacks the farm, he tries to fight it off unsuccessfully.
- In the Nickelodeon animated short, Jerk Chicken and Fish Out of Water in: Cop-A-Doodle-Doo, Jerk Chicken is an egotistical rooster who uses his position as a police officer to boss people around, including Fish. However, when it comes to dealing with actual criminals, Jerk Chicken pretends not to notice they're around.