Aperture Hand Lab: Thanks to the possibilities of the Valve Index controllers, the player can flip off the cores. The game actually acknowledges when this happens, with the cores reacting in annoyed/offended way.
Friendly Frank: That is NOT a wave; that is a finger! The unfriendliest finger on the entire hand! Angry Allen: Well, that is disrespectful. But it's not a shake, so I'll allow that. Business Bill: You're fired. Now you're rehired, probationally.
Anthony Williams from Bad Day L.A. does this to some rude drivers on the highway he's trying to collect food on.
In Battlefield 3 you have to perform a quicktime event to kill a rat. If you fail, the rat makes enough noise to interest a guard, who then comes over and shoots you. As your character dies, he manages to flip off the rat.
Battletoads (2020): The Dark Queen gives the finger to the Battle Toads using her left hand after they take off in their ship without her.
Bayonetta has a blink and you'll miss it moment where she flips off some angel that had just destroyed the road and then uses the finger as an ignition key for a motorbike.
The Binding of Isaac: One of the random daydreams Isaac will have when transitioning floors, added in Repentance, is him watching the clouds outside. He then notices that one of the clouds has changed to resemble a hand flipping him off. It sums up his life in the game's story pretty well.
Choking Hands in Blood II: The Chosen flip you off when they see you. Every time. Even if you just hide around a corner and pop out again before the animation cycle from when they flipped you off previously is done. Considering these hands are autonomous zombified body parts enchanted by what is essentially a Satanic cult, it's pretty funny.
BloodRayne does this after killing Wulf and Beliar before giving them the double bird in the second to last cutscene, then gives the finger to Severin when he tells her to try and keep it quiet while on their mission.
Bloodwings: Pumpkinhead's Revenge: It's possible to defeat Pumpkinhead (who is a One-Hit Kill boss) through brute force in the final stage and ignore the puzzle completely. If you manage this, you get a cinematic where he gives you the finger instead.
Done by a Headbanger to a group of Hairbangers after he and his friends join Eddie. IF you have the language censor on, it's covered by a big PMRC sticker.
Later done by Eddie to Doviculus when he attacks his living castle, twice, both times coupled with an And This Is for...:
Eddie:[after attacking one of the towers] That one was for Ophelia![flips Doviculus off with one hand] Eddie:[after attacking the other tower] And that one was for Lars! [flips Doviculus off with both hands]
Card City Nights: One random Flavor Text of the "Bird (Beta)" card, and a Pun referencing cards being flippable, although not in this game:
"Flip" this card? We can't write that!
In The Darkness II, if you shoot the car coming at you during the scene in the parking garage it explodes, and Jackie does this to the driver as he passes overhead before crashing into the wall and dying. You even get the achievement "The Bird is the Word" for pulling this (easy) stunt off.
Near the end of Mission 11 in Devil May Cry 4, Nero sticks out the middle finger of his Devil Bringer to Dante after the latter joked about Nero being "so melodramatic" as he gets absorbed into The Savior. And he does it again in Devil May Cry 5before activating his Devil Trigger in the final battle against his father Vergil.
In DmC: Devil May Cry, Dante gives the middle finger to demon news pundit Bob Barbas before their fight.
In Disco Elysium, when Garte, the hostel manager, confronts the Player Character with the payment he owes him for trashing his room, amongst other things, the player chose to can make a Savoir Faire check to attempt to run out on the bill. Failing the check causes the Player Character to, instead of just running away, get about half way across the room before spinning around to leap backwards towards the exit while flipping Garte off with both hands. He then crashes into another guest in a wheelchair, because he focused too much on the rude gesture and failed to see where he was going.
Disgaea 3: Absence of Justice has an interesting example: at two points in the story, the party battles the disembodied hand of Mao's father (said hand is large enough to stand in and hold several people at once, forming the stage). The middle digit is appropriately named "The Bird", described as "Cruel", and if it's the last unit to be fought, its "One-Man Army" Evility kicks in, doubling its stats.
The "final trailer" for Duke Nukem Forever showed Duke flipping off a big alien spaceship.
While in the game itself you do this after defeating a couple of bosses. Most notably would be when he finishes off the 'Queen Bitch' and just before losing consciousness, flips her off.
Slapping the Bile Demon in Dungeon Keeper will cause him to do the British version of this (the V with the palm facing away from the person)
Subverted in The Elder Scrolls Online. You can obtain an emote called flip the bird, however this emote involves your character taking an actual bird and flipping it in the air.
In Escape from Monkey Island, if you have Guybrush examine the appropriate part of the hand-shaped table in the Voodoo Lady's home, he'll remark "Ah, the middle finger. The most communicative of fingers."
Your character can do this in Fable, and in Fable II you can have a sculptor make statues of you doing it.
Family Guy Video Game: Betram gives Stewie the finger in the cutscene after the final battle with him.
Rex can do this in Far Cry 3: Blood Dragon by pressing the melee button without any enemies in range. He can even alternate hands if you do it fast enough.
In zOMG!, the Taunt ring draws aggro from nearby Animated. You flip one bird with three Rage Ranks, and you flip 'em both when you use full Rage. In both cases, the relevant fingers are blurred.
In Gears of War 3 Cole asks politely if one of the stranded have a vessel they could borrow, the response; "Here are the keys to my luxury yacht" while giving him to finger.
Grand Theft Auto III: Claude flips the bird when pedestrians honk their car horns at him.
Grand Theft Auto V: You can do this in place of being armed while inside vehicles by pressing the Shoot button. It is also a selectable taunt when playing GTA Online.
Grezzo 2, another Game Mod of Doom, has the same flip off mechanic of Brutal Doom; just replace the original taunts with random Italian insults.
Grim Dawn has the epic scepter item Finger of Gar'Dalvur. The scepter's head is a skeletal hand with the middle finger extended.
"The fallen Gar'Dalvur had only one thing to say to his executioners."
Guilty Gear: Sol Badguy does this as the Respect variation of his taunt, surprisingly enough. International versions censor this to a thumbs-up, making the taunt more "respectful", in a sense. (Ironic, considering that the thumbs-up can definitely also mean "up yours" in certain cultures.)
Pedestrians can do this in Infamous to Cole if they hate him or thieves that they hang with a rope by their feet which he can free.
I Was a Teenage Exocolonist: In one event where Sol beats Vace in a sparring match, the latter gives them the finger if they rub in their victory on him.
Daxter gives this to Pecker in Jak 3: Wastelander after yet another argument between the two, right in front of Jak, the king of Spargus, and an audience (who all gasp in unison). It was censored by showing it off-screen.
In Jak X: Combat Racing Pecker gives one to G. T. Blitz, this time censored by Pixellation (justified since it happens on TV).
In Kingdom Come: Deliverance, there is a side quest in Sasau called "In God's Hands". You talk to Johanka, who is taking care of the survivors of the Skalitz massacre in the Sasau monastery, who asks your help in healing the multiple injured people there. One of the wounded is suffering from insomnia; you can go to the alchemy bench and make him a sleeping potion. This can be repeated twice more on successive days, but the second time Henry (your player character) berates him for not saving any for later, and the third time says "you're welcome" and gives him a two fisted middle digit salute.
In Kung Fu Chivalry, the ending portrait of the bruised and battered Rogues Gallery depicts the female boss giving the player the finger.
Ellie does this to Bill in The Last of Us when he scolds her for messing with his magazine pile.
The Legend of Heroes: Trails of Cold Steel III has Ash Carbide giving Rean the middle finger after Rean and Ash talk about Ash's circumstances, Rean encouraging him to move forward, and Rean wishing his student a good night's rest.
In the second game, in the level "Pipe Dreams" in the Factory of a Better Tomorrow, there's a moment where you have to pull levers to move fingers on a hand to form the hand that rockers use. However, you can also choose to put up just the middle finger, which will cause a sign that reads "Denied" to cover the offending finger until you put up another finger or take the middle one down.
In the "The Journey Home" level kit in the third game, one of the levels is based on the Factory of a Better Tomorrow, and the hand from the above paragraph makes a reappearance in a 2-player section. You have to put the pinky and index fingers in the same position that you had to in the second game, but you can also just put up the middle finger again, causing another "Denied" sign to come down and Clive to say "Nice try. We have policies against that."
Lollipop Chainsaw: At the end of the fight with the first boss, Zed, Juliet cuts off his hand, which lands in the appropriate position.
In what may be the best possible encapsulation of her character, Mariska gives Juliet the finger, and then turns her hand around into a peace sign.
Loopmancer have the villain Wei Long's last, Defiant to the End moment, where he repeatedly cusses you, threatens that his underlings will make your wife suffer, taunts you over your daughter's death and finally finally flipping you off. You then rip out his medulla oblongata with your bare hands.
In the World Builder game LS Jumble, one of the player's default attacks is "Raise Middle Finger".
Jack Cayman of MadWorld has a variation of this, though he replaces "Middle Finger" with "Chainsaw from his arm".
Meat Boy: Dr. Fetus is quite fond of this gesture, to the point that his biometric hand scanner in Forever is programmed to scan his middle finger pressed against the screen. Meat Boy and Bandage Girl's daughter Nugget at one point even copies Dr. Fetus by flipping off butterflies.
The "Sweet Goodbye" achievement in Mirror's Edge. This is achieved by jumping forward, doing a 180 degree turn while in the air and then pressing the attack button in quick succession. This will make Faith flash a two-finger salute while falling backwards.
Need for Speed (2015) has Amy, the mechanic of Travis's crew, who occasionally gives the middle finger to her own mates.
Pizza Tower: During the boss fight with The Noise, one of the poses he can strike when vulnerable to attack has him flipping Peppino off. The explosive dummies The Noise drops in his second phase will also flip Peppino off.
Pokémon Black 2 and White 2: Not in the game itself, but early concept art for Roxie showed her using her middle finger to do a Eyelid Pull Taunt. Evidently, another member of the art team realized this and had it changed to something completely different.
Postal 2 has NPCs do this on occasion when annoyed, whether at you or another NPC. The Paradise Lost DLC allows you to do it, too, by pressing the button normally used to shout at people to get down without a weapon equipped.
The Japanese version of the TurboGrafx-16 shooter Psychosis has one from the creature seen in between stages.
In Rainbow Six Siege, Dokkaebi gives the reverse V sign to Thatcher. after that training.
Thatcher:Don't worry, maybe we'll get them right next time. Dokkaebi:Two more seconds and we would have had eyes in the room. Thatcher:They were already on cameras. That's two seconds too slow Thatcher slams the door in front of Dokkaebi then Dokkaebi gives him a V-Sign in reverse.
In Scott Pilgrim vs. The World: The Game, Todd does this a lot — even in the pre-fight closeup. Kim does this as well, but only if her hand is behind another character's head; if she's the only player, the expression becomes a thumbs-down.
South Park: The Fractured but Whole has a few aggro control moves, some of which involve this. Craig's Shining Hate Finger has him flip off a single target, while the New Kid's "Dragon Swagger" summons a spirit dragon that flips off everyone with both claws.
Implied in Spiritfarer when Gwen tells off the first raccoon shopkeeper Stella meets by telling him that he's "the reason everyone has middle fingers".
Of all games, Super Smash Bros. Brawl has Wolf flipping off the camera in his intro when you fight him in Adventure Mode.
In Surgeon Simulator 2013 you control a seemingly disembodied hand and are given the ability to control each individual finger. Obviously, flipping the bird is what most players do at one point or another. There's even an achievement for that.
During episode 5 of Tales from the Borderlands, Rhys gleefully flips the double-birds towards the monitors displaying Holo-Jack as he escapes through the hallways of Helios. Even better is that this was apparently an accident — the writing team wrote in the script “Rhys flips off the monitors as he runs by”, meaning that he physically switches the monitors off, but the animators misinterpreted this and instead made something that was deemed too funny to correct.
In Tales of Xillia, Agria gleefully does this to Leia as she falls to her doom after rejecting the latter's attempt to save her. Though only in the Japanese version of the game.
Paulie 'Wheels of Fury' Ryan salutes the Player Character this way when teams are chosen in Tony Hawk's Underground 2. It's blurred out, though, because the game's rated T.
The gesture is also a Mythology Gag for the Madness Combat fan game, "Madness Accelerant" which had Tricky doing the same gesture.
Turbo Overkill, an FPS, have you flipping off your enemies after a high kill-streak (seen from a First-person perspective). With your robotic left hand.
, the ending portrait of the bruised and battered Rogues Gallery depicts the female boss giving the player the finger.