A young scientist who brings his dog and best friend Sparky back to life.
- Badass Bookworm: Managed to bring the dead back to life.
- Brainy Brunette: Successfully able to resurrect his dead dog.
- Expy: Victor is a smaller Victor.
- Meaningful Name: His last name comes from a scientist who brought a dead body back to life.
- Nice Guy: A kind and friendly young man.
- Science Hero: Victor not only uses his vast knowledge of science to help revive Sparky, but also to stop the other kids' messed-up resurrections.
Victor's pet dog that he brings back to life.
- Action Pet: When he takes on the mutated Mr. Whiskers.
- Body Horror: Sparky's body has stitches, holes, and his tail comes off.
- Official Couple: Possibly with Persephone by the end.
- Papa Wolf: A near literal example. Sparky goes into the burning tower to save Victor.
- Secondary Character Title: He's "Frankenweenie", but the real protagonist is Victor.
- Undying Loyalty: No pun intended. To Victor to the extent he would rescue him from a burning windmill.
- Cute Bookworm: Susan is an avid reader.
- Good Parents: They are encouraging, supportive, and understanding to their son, Victor.
- Happily Married: They have a loving and playful relationship.
- Housewife: Susan appears to take on the role of stereotypical cookie-cutter '50s housewife.
- Mama Bear: Along with her husband, she would have charged straight into a burning windmill to save Victor had the other adults not held her and her husband back.
- Nice Guy: Edward is a friendly and social, and likes to dispense roundabout advice to his son. Susan is a friendly, loving and supportive mother who encourages her son's scientific pursuits, even when her kitchen appliances end up in his attic lab.
- Papa Wolf: Along with his wife, he would have charged straight into a burning windmill to save Victor had the other adults not held him and his wife back.
A kind next-door neighbor, and one of Victor's classmates.
- Broken Bird: Downplayed. Elsa is described as somber and she's forced to live with her unlikable uncle Mr. Burgermeister for awhile.
- Girlish Pigtails: Averted. She has four pigtails, but doesn't appear to be very girly.
- Nice Girl: Comforted Victor after the death of Sparky.
- Perky Goth: Downplayed, since her personality is a bit on the shy side.
- Puppy Love: With Victor.
- Shrinking Violet: She's rather lonely, quiet, shy and a bit withdrawn.
Elsa's pet poodle.
- Beehive Hairdo: After Sparky "sparks" her.
- Captain Ersatz: Of the Bride of Frankenstein.
- Official Couple: Possibly with Sparky in the end.
- Satellite Character: We only know that she's Elsa's pet poodle.
- Satellite Love Interest: Also serves as Sparky's love interest.
- Skunk Stripe: After Sparky accidentally electrocutes her.
Victor's rival-like former enemy and one of his classmates.
- Asian and Nerdy: Very intelligent for his age - he fully understands Victor's calculations, which could be why his experiment is so 'successful'.
- Asian Speekee Engrish: Slips into this.
- Brains and Brawn: The Brains to Bob's Brawn.
- Expy: Various major Japanese characters from Godzilla and other kaiju movies (general form) and The Night Porter from David Lynch's The Elephant Man (personality.)
- Token Minority: The only major non-white character in the story.
Victor's other main scientific rival.
- The Comically Serious: He is so darn melodramatic and exaggerated about everything, that he ends up being hilarious in a pompous sort of way.
- The Cynic: He takes a darker, more doomsday view of life than the other kids in New Holland. Nassor is instantly skeptical of the rumors surrounding Victors experiments with electricity.
- Jerkass: Indifferent, bleak, power hungry, and dark.
- Foil: To Victor.
- Large Ham: Especially after Colossus is reanimated.
- Perpetual Frowner: Nassor never seems to smile and keeps a gloomy attitude no matter what happens.
- What Happened to the Mouse?: Nassor is comedically turned into a living mummy by stumbling into some ribbons while trying to run from Shelly Toshaki's undead Kaiju turtle and than falling into a Russian Nesting Doll Case which closes on him and becomes his makeshift coffin. The audience doesn't see him after that and his fate is unknown. He could potentially be Buried Alive. Forever.
A hunch-backed child and one of Victor's classmates. He's the first to know of Victor's success in bringing Sparky back to life.
- Creepy Long Fingers: His left hand is deformed, giving him extra long digits.
- The Igor: Defied; he's not friends with Victor at all, and blackmails Vic into showing him the resurrection experiment. About the only thing about him that fits is that he's disfigured and has a weird voice.
- I Just Want to Have Friends: Edgar only wants to make a couple of smart friends.
- No Celebrities Were Harmed: His design and voice patterns were inspired by Peter Lorre.
- Unwitting Instigator of Doom: If Edgar had just had kept his mouth shut about Victor reviving Sparky, then the town wouldn't have had the monster epidemic.
An eccentric girl who is one of Victor's classmates and obsessed with the psychic predictions of her cat, Mr. Whiskers - which do actually seem to be correct.
- Creepy Child: She delivers ominous pronouncements in a monotone voice with an unnerving stare.
- No Name Given: Only goes by "Weird Girl."
- Oracular Urchin: A superstitious parody who rambles all the time about her cat's prophetic powers. Needless to say, she doesn't fit in well with the other kids in New Holland, especially when she delivers her ominous pronouncements in a monotone voice with an unnerving stare, about them.
- Waif Prophet: Rail-thin kid who finds portents in her kitty's sandbox.
An obese boy who is one of Victor's classmates.
- Big Fun: One of the major sources of comic relief.
- Brains and Brawn: The Brawn to Toshiaki's Brains.
- Momma's Boy: Loves his mother very much.
- Recycled Character Design: He looks identical to a fat kid with black-and-white striped pajamas to whom Jack Skellington gives a monstrous toy in The Nightmare Before Christmas.
An eccentric but wise science teacher at Victor's school who speaks in a thick Eastern European accent. His teachings inspire Victor's effort to resurrect Sparky, and he acts as a mentor to Victor.
- Eccentric Mentor: Deconstructed. He encourages his students to be creative and eccentric when it comes to science. When one student (Bob) gets hurt while doing so, the parents demand he be fired. He is.
- Nice Guy: An eccentric teacher who genuinely wants to inspire his students to love science.
- No Celebrities Were Harmed: His design was deliberately based off of beloved horror icon Vincent Price.
- Soviet Superscience: Invoked, everyone in his homeland is a scientist.
- What the Hell Is That Accent?: Has an eastern European accent and You No Take Candle speech pattern.
Weird Girl's pet cat who can apparently tell the future of people according to his owner.
- Big Bad: There is a popular fan theory that maintains that Mr Whiskers was secretly manipulating the events of the film to ensure that his psychic visions of Victor and Sparky came to pass
- Bat Out of Hell: He accidentally fuses with a bat in the climax and as a result becomes extremely murderous.
- Body Horror: When Mr Whiskers supernaturally fuses with a dead Vampire Bat he becomes a Hideous, Blood Thirsty Mutant Hybrid.
- Cats Are Mean: Averted for most of the film. He really becomes this after his transformation in the climax.
- Impaled with Extreme Prejudice: By a flaming rafter in the climax. Overlaps with Family-Unfriendly Death:
- Final Boss: Is the final antagonist that Victor and Sparky have to face
The town's grumpy, disagreeable mayor.
- Adipose Rex: He has a stout, portly body frame as Mayor of New Holland.
- Captain Ersatz: Of Burgermeister Meisterburger from Santa Claus Is Comin' to Town.
- Grumpy Bear: A real grouch of a man.
- Literal Metaphor: A Dutch uncle is someone who issues frank, harsh, or severe comments and criticism in order to educate, encourage, or admonish someone; he insists that his niece Elsa is to be the star of the New Holland Dutch Day festival.
- Meaningful Name: "Burgomeister" is the Dutch word for "Mayor."
- Papa Wolf: Gets Mr. Rzykruski fired when Bob hurts himself trying out a jetpack he and Toshiaki built for the Science Fair.