The Fleischer cartoons that pioneered rotoscoping can fall into this at times. Just look at Bamboo Isle, in which Betty Boop's massive, misshapen cranium is put on an actual hula-dancer's body. There it was probably unintentional, but everything about Minnie the Moocher seems precisely calculated to terrify, from the use of rotoscoping to make a walrus-man ghost dance like Cab Calloway to the dark lyrics of the song he sings to, well, everything else in the cartoon. Take a look here.
This trope is brilliantly alluded to, and parodied, in the episode "Frankendoodle." The drawings of the characters that come to life are described in-universe as being "kinda creepy when they move." And Patrick is obviously put off by Doodlebob because he resembles, but never entirely imitates, Spongebob. In universe, Doodlebob is seen as disturbing, unsettling, and creepy.
In a Transformers example, though it's not obvious to the viewer due to the animation style, this is implied to be why Transformers Animated's Sari is despised by other children.
One of the many complaints leveled against Beast Machines are the designs of the Maximals, all of whom have very humanlike features despite being alien robots. Nightscream is the worst offender: unsightly, scrawny body, a hunchback, Creepy Long Fingers, an incredibly humanlike face with gigantic, widely-spaced eyes, nostril-like holes on his forehead, and a clump of actual hair dangling in front of his face, which looks like a piece of flesh due to the CGI's limitations. Blackarachnia is a close second: her extra eyes deliberately invoked this, but when closed, the give her a giant forehead. Her hair also looks like a clump of tissue.
Skyland, a motion-captured 3D-modeled cartoon, attempts to make things look more stylish by cel-shading it afterward. This backfires, though, making the characters look inhumanly polished and sending the entire thing plummeting into the Uncanny Valley.
Letter TV, a low-budget CGI educational program for children, seems to have fallen squarely into the valley's nadir.
Math Girl: A series of semi-animated CGI shorts with writing that seems aimed at children, subject matter (calculus) aimed at high school and college students, and extremely frenetic, jerky pacing that makes actually learning anything unlikely. The characters are right in Uncanny Valley, helped by the creepy Exorcist-style theme music. The temptation to shut it off or vocally make fun of it is nearly unbearable, but unfortunately, this is rather hard to do in a classroom setting. Here's episode 1.
Courage the Cowardly Dog was a very frightening cartoon for many, often skipping the "comedy" part of its "horror-comedy". By far, the most frightening episode was the Grand Finale: "Perfect". Courage had a series of nightmares where he was declared "not perfect" by what appears to be a blue half-human, half-octustingray thingy◊.
In the episode "The Mask". Kitty before removing the mask qualifies, and the atmosphere and music certainly doesn't help. "Doc Gerbil's World" is equally mindrending.
Punsy McKale's realistic design looks a bit...off to say the least.
Also parodied in an episode of Chowder with a pink, badly CGI'd dancing pig-baby (probably a parody of the infamous CGI Dancing Baby), whose mere existence instills great horror (and grief) on those who watch it.
Also invoked for many of the villains in The Real Ghostbusters. One of the most traumatic characters for anybody growing up in the 1980s might have been their Boogey Man.
Sid the Science Kid is somewhat creepy. The reason is Henson Studios using a technique that combines CGI Motion-Capture with actual puppetry. The actors are "filmed" in real time as the CGI characters, with as little post-production as possible being used.
In-Universe example: Homer briefly imagines his children as "mutants" with pinkish skin, five-fingered hands and eyes with irises. The camera briefly cuts to a more realistic rendering of Bart, Lisa and Maggie. Homer screams and runs away.
The original show has nothing on the depths into the Uncanny Valley the fans will boldly go. Michael Swaim gives us The 7 Creepiest Simpson Fan Tributesnote Or "The 7 Most Extreme Forms of Simpsons Fandom", if you want to go by the in-video title.. The title is most certainly accurate.
In the episode were Gabbo was introduced, Krusty tries to compete with his own ventriloquism number, using a puppet that looks rather creepy. And then the puppet loses its lower jaw. From this point on, things go downhill rather quickly.
This Butterfinger commercial shows Homer from an angle that doesn't make him look like Homer anymore.
A throwaway gag in the season 5 episode "Rosebud" has George H.W. Bush rejected from Mr. Burns' birthday celebration for only serving one term as president. His caricature is mildly offputting, especially when he says "Get away from me, loser" to Jimmy Carter.
Many of the celebrity guest appearances come across as this, basically looking like traced photos with minimal to no effort to make them fit in with the Simpsons art style. The result is often incredibly unsettling.
In the 1960s there were action animated shows (often based on Marvel Comics) that actually were not animated at all. Yes, apparently they reached a new low in how to be cheap on animation. But how did they handle the characters talking? Simple! When a character spoke, that character would be a still drawing with a human mouth on the face speaking. This is called Synchro-Vox and it gave off what is arguably the first and one of the creepier examples of Uncanny Valley in North American animation. Say what you will about the 1970s being a Dark Age for TV animation, but by that time that practice was no longer the least bit common. (Nowadays it's only very rarely used for comedic effect and for The Annoying Orange series, where two of the same human eye and a human mouth are used to make talking inanimate objects.)
Clutch Cargo used the same technique, and started airing in 1959. The technique is called "Synchro-Vox" and was invented in 1952.
Intentionally invoked in Batman: The Animated Series, part two of "Heart of Steel". The robotic replacements for Gotham's citizenslook perfectly normal, but move in increasingly impossible ways - such as their heads spinning around to look at things, or crouching and leaping in a terrifyingly inhuman manner.
The Night of the Headless Horseman 1999 CGI movie. Stiff 3D models attempting to be lifelike with motion capture, and cartoon-like with severely distorted expressions, combined with over the top voice acting, may cause the presentation to be scarier than the story.
In the episode "Knock It Off", the Big Bad creates an army of PPG imitations, but with shoddy worksmanship. The scariest ones are those who are only slightly different from the originals.
Arguably, the Powerpuffs themselves. As the characters are designed to look ridiculously cute, they have rather big eyes, and a lack of fingers, toes, noses, and ears. A number of jokes in the show revolve around their appearance because of this, and there's even an episode that showed what would happen if the Freak Lab Accident never occurred and girls turn out to be the Run of the Mill Girls instead, without their unique cuteness features.
Video Brinquedo defies the Uncanny Valley chart quite a bit. Despite the fact that the human similarity to characters is as big as in any cartoon characters, their familiarity index is much lower than it should be.
Timmy: No amount of therapy will ever make this moment okay.
Anyone who's ever taken a German class and had to sit through episodes of the series Hallo Aus Berlin will have been subjected to Rolli und Rita, the creepiest creations ever to come out of computer animation. See for yourself.
Scooby-Doo, Where Are You!: In-Universe, this is how the Funland operator◊ of "Foul Play in Funland" is seen by the rest of the gang, who, throughout the episode, make it verbally clear his eerie atmosphere and superhuman abilities are absolutely off-putting. Of course, the revelation that he's just a haywire animatronic certainly helps.
Parodied in an episode of The Tick where a department store security guard complains that the store's animatronic Santa Claus is re-appearing in his nightmares. This gets aggravated when Santa's head gets torn off and thrown into his lap.
Invader Zim had the Irkens looking better than the humans. Dib, Gaz, and some of the adults could pass, but a lot of the other adults were....creepy. That lady with the weird boil on her head who lived next door to Zim may have been more scary that the invading aliens.
Bionicle: The Legend Reborn used the actual LEGO toys as its character models with an absolutely minimal amount of tweaking, to make them seem more realistic while still keeping the intended "toy look". One added feature was the rotten-looking teeth and weird lips formed by a second row of mechanical teeth that slid over the real ones, but their true jaw-line still remained apparent. When they smiled, it looked horrifying.
The earlier movies also had shades of this, not with the designs (which did their best to avert this by redesigning the characters from their toy looks) but the animation itself. The movements in the first two were often very choppy, with the characters turning stiff a lot. Then, there are Tahu's cheeks, which flap like tissue as he performs a skydive inside a volcano, despite his mask being supposedly solid metal. Given that the mask has an odd, organic-looking design with many tubes running across it, this flapping makes it look like he has his facial muscles exposed.
The Delightful ChildrenFrom Down The Lane in Codename: Kids Next Door are pretty much in and out universe Uncanny Valley fuel, due to their Voice of the Legion, blank eyes, and sociopathic qualities, not to mention a slight Non-Standard Character Design. They're arguably the scariest aspect of the series. Lenny (the football helmet) averts this when he betrays them for the K.N.D. He's still a bit weird, but seems like a normal kid with a nasally lisp thanks to his headgear. Then he dives headfirst back into the creepy hole when he reveals himself as a triple agent. He slides back into his place in the group with a slurping sound.
Franklin in the Franklin And Friends series. It's an All-CGI Cartoon but the animation is actually pretty top quality. Everyone looks fine... Except for the turtles◊. The series is going for a realistic look but the turtles are just too cartoony.
Rugrats: The original pilot. The animation is much more detailed and makes the characters look ugly or downright monstrous, especially the necks. It's also present in the first opening, used for six years and long after the animation and art style had become more pleasing to the eye.
This poster for a live-action Marsupilami movie in France.
Allen Gregory: The characters just look off. Allen himself suffers the most from this. He looks like a ventriloquist dummy with huge Vocal Dissonance.
My Life Me: The characters move like marionettes and the character designs just look weird.
Some video series for little kids involves insects called Hermie the Caterpillar, an Animated Adaptation of a series of books by Max Lucado. Some of the bugs are just a little bit creepy, thanks to No Flow in CGI, but special mention goes to two ladybugs named Hailey and Bailey whose eyes are just too big for their vaguely human-looking faces.
From My Little Pony generation 3.5, the Newborn "Cuties". Besides looking like they had a permanent "duck face," the size of their eyes compared to the rest of their bodies looks a little bit off.
Season 2 is riddled with shots which slip into the valley, usually head-on shots of the characters' faces. One suggestion as to the cause of this is the animators for Season 2 making the mouths substantially wider than was done in Season 1. For some of the worst offenses, take a look at this series of screenshots◊ from "May The Best Pet Win".
Ms. Harshwhinny is a unique case, being a character who was drawn to match the standard mare proportions and designs but doesn't reuse any flash assets and instead has a complete set of uniquely drawn expressions, body parts, eyes, etc. Put her beside a mare who does use the standard assets, and she kind of sticks out.
Some of the human characters in My Little Pony: Equestria Girls and its sequels wear lipstick, most prominently the human counterparts of Celestia and Luna. This wouldn't be so bad if their lips weren't then outlined, which has the unfortunate side effect of making it look like something is very wrong with their mouths.
The season 5 premiere does another in-universe example with the ponies who have supposedly found happiness in being fully equal. Pinkie Pie's first reaction is to say that there's something wrong about their smiles.
Family Guy suffered from the uncanny valley for the first few seasons until it got popular enough to have a bigger budget. Most of the characters moved quite stiffly and facial expressions were also just as stiff. Peter also suffered from glitches in the animations, such as his eyes suddenly growing bigger or how his tongue stuck out in odd places when he talked. As the series progressed, the characters' movements improved and their facial expressions are a lot smoother. This eventually gets lampshaded by Brian and Stewie when they go back in time to the pilot episode and they witness the oddities themselves.
Any FuturamaFan Art that portrays remotely non-human characters in a more realistic drawing style. It's a given that if Leela's eye wasn't a simple oval and black dot, Fry would be unable to look at her, much less have sex with her. Actually, that's probably how many people in-universe see her. From "The Cyber House Rules"...
Leela: No one's stared at me, or avoided staring at me, or tried to burn me!
Adventure Time makes use of this trope a lot when creating a character who is supposed to be off-putting:
Lemongrab. In a show where almost all the characters have black dots for eyes, his eyes are jarring. Lemongrab, with his Non-Standard Character Design, has realistic eyes- white ovals with dark pupils, Dull Eyes of Unhappiness that just look wrong when they're not exaggerated into cartoonish outrage or fear.
The scene of Lemongrab's creation, and coming to life, deliberately invokes this trope. The reaction Princess Bubblegum has to her failed experiment is the same reaction the audience has to him, too.
Goliad has the voice of a little girl and the head of a baby, and uses People Puppets as a means of ruling the kingdom. These generic cute characteristics paired together with the body of a sphinx make her incredibly unsettling. It doesn't help that Goliad is also HUGE, and runs/leaps like a lion would. That eye that comes out of her forehead notably makes her face even more disturbing, to the point where Jake actually gets disgusted.
Marceline's Dad moves like a marionette on mismatched strings- his walk is just creepy.
Princess Monster Wife. The Ice King assembled PMW from body parts stolen from his favorite princesses. Imagine Frankenstein's Monster, but as a big, bulky princess with grossly mismatched arms, legs, and parts of her face. Her voice consists of three simultaneous voices of the princesses who compose her mouth. The most unsettling scene with PMW is when she is trying to eat. She lifts a spoonful of mashed potatoes to her mouth, but she is unable to eat because her mouth is so misshapen. (The fact that Princess Monster Wife is a sweet, kind, and genuinely sad character softens the blow of the uncanny valley a little bit, though.)
Guardian Angel has cartoonish eyes mixed with a realistically drawn, skeleton mouth.
The Scrodinger's Cat/Demon Cat thing with the broken, floating legs.
The Lich is a skeleton half-covered in dead, tattered flesh that moves. Lich-posessed Princess Bubblegum and Lich-posessed Snail deserve special mention, too.
Some fans had this reaction to Susan Strong. She has the same simple face, eyes, and head as the other characters, but has a disproportionately large body, and it is a very muscular, realistically-drawn body. Unlike the other examples, she was intended to be appealing- not unsettling.
Some of the more non-cartoonish expressions, in a similar manner to the infamous "She's Here" scene of Flapjack... such as Finn's "Four Weeks" face, and that horrifying face he makes when a drop of water comes towards him.
Ricardio is a cartoonish heart with the realistically drawn face of George Takei.
Sometimes, Cinnamon Bun falls into this trope, because of the way his mouth is drawn. His green eyes (prior to his redesign) were creepy, too.
The 2010 reboot of Pound Puppies. Its art style is a odd mix of "cutesy" and "cartoonish" that often doesn't mesh well. The animation has improved significantly, thanks to production being moved to the same company that produces My Little Pony Friendship Is Magic, but the first seven episodes are quite... interesting in their animation style.
The designs of the main six from the original pilot from Recess certainly falls into this (unfortunately, there's no image available). And while the whole thing hasn't been shown to the public, from the clips that were shown, the series pilot could also fall into this for those who are really used to the character designs in the show, as the gang were going to be more realistic.
Almost all of Toon City's episodes fall straight into the valley.
Just about every short on KaBlam! except Sniz & Fondue and The Offbeats. The worst offenders would have to be Garbage Boy and The Shizzagee from season four, the latter especially for the CGI which not only hasn't aged well, but was outdated even from the beginning when it was made in 2000, making it look slightly better than Video Brinquedo.
For some viewers, the animation style of the Life With Loopy shorts tend to have this effect, though the fans will argue that after a while, it's just something you get used to. The pilot ("Goldfish Heaven"), however, falls much deeper into the valley due to it's even cheaper look and rougher animation. This one shot of Larry laughing◊ falls there the deepest.
While the characters on King of the Hill are mostly in line, there are a few occasions where they slip into this trope, like some of Hank's "bwaah" faces from a few early episodes.
Disney's Electric Holiday's catwalk sequence shows some Disney characters with skinny bodies. While it wasn't so bad with the human characters, the Funny Animal characters look a little off.
Teenage Mutant Ninja Turtles (2012) seems to intentionally do this to the Kraang, where, while stylized, the Kraang's disguises are always a simple, droll businessman, whose face and eyes almost never move. It's sort of creepy.
The infamous ending bumper to [adult swim] featured a pair of bleary eyes being greeted by the grinning sun from behind a hill, and the text "THE DAWN IS YOUR ENEMY". The creepy drone music didn't help matters either.
Thomas the Tank Engine & Friends is this to some fans after the show's format was converted from using model trains to CGI with the characters actually moving their mouths while they talk.
Even worse than CGI Thomas was the short-lived pre-schoolers' show: Jay Jay the Jet Plane. Imagine Thomas the Tank Engine, except the trains are planes instead and they have more human-like skin tones and facial features instead of Thomas' completely white faces and cartoony features.
This is pretty prevalent in Storm Hawks Every person and thing moves awkwardly and resembles really, really, really poorly built robots.
Most anything drawn by animator Peter Chung, which includes works like his well known MTV cartoon series Ĉon Flux, and for doing animated stories for The Animatrix and the Dark Fury special in the Riddick series (also Reign: The Conqueror, but that one's an anime example due to being a Japanese/Korean co-production). Most of his characters (like Aeon Flux herself) look downright anorexic, and as Pan Pizza from Rebel Taxi put it "is it possible to have TOO MUCH animation?", stating that the style really takes some getting used to.
One of the most common complaints about the character designs in Code Lyoko is that the foreheads of most every character looks gigantic, and is often the butt of many a joke made by both fans and non-fans alike. Most people are usually able to look past this oddity once they really get into the show's character driven storylines (and later more complex plots).
Archer: The stiff animation and semi-photorealistic character designs can cause this reaction in some viewers.
Most of the human characters in George Pal's Puppetoons have cartoony and simplistic designs, so when a "realistic" one appears it tends to come across as unsettling.
The Monster High movies try too hard to recapture the dolls' looks in CGI, and the results just look wrong most of the time. Luckily its spinoff Ever After High avoids this by sticking mostly to stylized Adobe Flash animation.
Xavier: Renegade Angel has a graphical style reminiscent of early 3D games, down to all the imperfections in the models and movement. As a result, everything looks disturbingly alien.
Butt-Ugly Martians fell very deeply into this. One can argue that the titular martians is Stylistic Suck, but the humans are not. Keep in mind back in 2002, the CGI was considered poor.
Strange Frame: Love & Sax features has hand-drawn animation that tries way too hard to look realistic. Also not helping things is the fact that it takes place in a future where genetic and cybernetic alterations are commonplace, resulting in a lot of characters who are nominally human but have extra limbs, doubled eyebrows or fur.
Titanic: The Legend Goes On has the audacity to rip off numerous Disney and Don Bluth character designs, but does it so incompetently that when the designs aren't just plain Off-Model, they're downright unsettling. There's something rather unpleasant about the human faces in the movie—when they're not crudely stylized, they're instead vaguely realistic. The problem is that when they address the camera in a scene, the low quality of the art and animation gives them a sort of floppy, choppy feeling that is definitely inhuman. This is not helped by the artists doing such a lackluster job with the faces, which resulted in many of the cast having Fish Eyes.
The 2015 reboot of Popples. Overly detailed human eyes with pupils, irises, highlights, eyelashes, and unnaturally stretched-out sclera on brightly colored Cartoon Creatures just doesn't look right.
Zack and Quack: Although the characters are supposed to look like they're in a popup book, it's not hard to feel they just look weird.
Something about the animation is not quite right. The series costs a lot (over $11 mil. USD) and it shows. The characters are very expressive but the character designs combined with this expressiveness can make them look uncanny. Certain characters also have a plasticy look to them. The original animesque 2D trailer was far less in the valley.
Rose is really freaky looking compared to everyone else. She has huge eyes for some reason.
Some of the exploring outside interstitial segments in latter installments of Wild Animal Baby Explorers feature someone wearing a full-body Sammy the skunk suit. While it seems to entertain the real kids featured in these segments, it can definitely have this effect for adult viewers.
Also played intentionally in any episode involving Rose's Room. The room can accurately represent Steven's views of his friends and family even managing to fool him with a replica of Connie, but this trope comes into play whenever it attempts to replicate too much, such as the whole of Beach City, or a complete personality. The resultcanbecreepy, if well-intentioned by the eponymous room.
Done specifically when Bill Cipher possesses Dipper's body to make things all that much creepier. As such, Dippers facial features become slightly more detailed◊, to the point he enters this territory. What's worse, he didn't stop smiling.
Descendants: Wicked World is an animated series that showed the worst of computer animation ever shown on screen. The character designs tried way too hard to capture the classic Disney style onto the cast of the 2015 TV film it is based on, and it does not look right whatsoever, and what is even worse is that the result ended up being too horrific for children to look at.
Some of the characters in BoJack Horseman may come off as this considering that many of them are fully anthropomorphic animals. The human characters vary with appearance, but Vanessa Gecko's appearance seems more off than anyone else.
There are many college animation demo reels that fall under this category. For instance: Crusaders the 2006 CG animation reel had different versions of the same two characters (the rock guy and the 242 girl) from both the picture and the separate parts of the video. The 242 girl especially given the animator tried so hard to use her emotion to look stern or angry; plus her big boobs differ; and at one point, jiggle awkwardly; even a couple shots show her visible cleavage window. Her different appearance may be a subject to Wardrobe Malfunction. (video and profile picture◊ here.)
This may be the reason why the Bratz cartoon never got that popular - the character in CGI looked downright horrifying.
The CGI of Shadow Raiders does not hold up by today's standards - the animation resembles 3D video game cutscenes, while the main characters look like they have either been chipped out of solid stone or are related to nuts.