Contrast Flying Dutchman (another spooky means of transportation, but forbidden to go to the afterlife) and Ghost Ship (for normal derelict vessels).
Not related to the TV show Soul Train (though both do involve both gettin' on up and gettin' down). As a Death Trope, all Spoilers will be unmarked ahead. Beware.
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Anime & Manga
- Digimon example: In Frontier (Season 4), Takuya found himself confronted by Dark Trailmon, a train Digimon, after getting annihilated in a battle. Dark Trailmon offers him a chance to avoid going to the Digital World, and takes him back in time to when his journey started. But the catch? He's running around the human world as Flamemon. (His wearing a "rookie level" version of his Digimon form is a way around the problem of two Takuyas existing at once.) He eventually decides to allow his journey to take place, and returns to the Digital World. (And the bad guy they were fighting has long walked away to sort out his lost memories by the time he got back. Oh, well. Next time.)
- ARIA has the appearance of a ghostly train that appears to transport cats to some sort of afterlife. This is a Shout-Out to Night on the Galactic Railroad.
- Sister Rosette Christopher in Chrono Crusade finds herself riding on one of these after she dies. Thanks to some words of encouragement from another passenger, she leaps off the moving train before it reaches its destination, causing her to revive in the real world.
- One season of The Gregory Horror Show had a bit of this. One passenger Gregory encountered was the ghost of a man continually re-living his daily commute to work. The kicker? Said man is the first protagonist.
- And while he wasn't dead yet, a giant chicken was unknowingly traveling to a slaughterhouse for his new "job."
- Shaman King has Matamune, the split-tailed cat spirit, appearing in the afterlife and boarding a train. The image is an homage to Kenji Miyazawa's Night on the Galactic Railroad.
- At the conclusion of the series, all of Yoh's friends and family arrive on a train connected by their souls to save him and the other four Elemental Warriors from being overpowered by Hao.
- One chapter in Princess Resurrection had Hime and the gang having to board one of these while fighting one of her siblings.
- Hakaba Kitaro has the titular character put two other characters on a soul train as part of a hallucination.
- Ghost Sweeper Mikami has a ghost train episode.
- Neon Genesis Evangelion: When he's absorbed into the Dirac Sea, Shinji's mind (we think) conjurs up a phantasmal train that is already in motion when he appears on it, and never, in turn, arrives at a destination. While aboard, he speaks to a second, unseen presence who claims to be himself. Whether this other him is in fact the God-Emperor of Mankind remains a subject of debate.
Rei and Asuka both end up in the Hell Train. The real mindscrew is when, later, Touji visits the Hell Train...and sees Shinji and Rei in an adjacent car of the same train (but the train is a type that doesn't allow passengers to move from car to car). His only comment is wondering what they are talking about.
- Galaxy Express 999 has an encounter with a train full of ghosts in the vicinity of Filament, a planet that had been suddenly destroyed some time ago leaving the souls of its inhabitants to live on in that area.
- At the beginning of Grave of the Fireflies Seita dies in a train station. When the tin containing his sister Setsuko's remains is thrown away, her spirit is released and the spirits of Seita and Setsuko reunite. They then board a lonely train, and watch falling bombs, which segues into the story of how they died with the bombings of Kobe.
- In Devilman, a train enters a tunnel that is actually a demon, with the conductor corrupted and taunting. Among the devoured casualties is a little girl who was once the only friend Akira Fudo ever had. A Fate Worse Than Death awaits the fallen.
- Zekkyou Gakkyuu: The bus in "The Bus To the Underworld," (translated as "Bus To Hell" by some groups, though it actually goes to a generic afterlife, rather than Hell). Apparently you can board even if you're not officially dead, as Miku, the protagonist boards it and wakes up in her house after refusing to get off at the stop, with her family revealing she collapsed at the station and was in a coma for three days.
- The episode "Eternity" from The Galaxy Railways features one of these. It comes at midnight every few months to a specific platform; you ride it until you're ready to move on.
- In the B.P.R.D. story "Night Train", there's a spectral train full of ghost soldiers. Back in WWII, the train had transported soldiers, until a Nazi saboteur destroyed a bridge, wrecking the train and killing everyone on board. In the modern day, the train and its soldiers hunt that Nazi to drag him off to the afterlife for judgement.
- A Superman arc sees Jimmy Olsen way way down on his luck, and missing a job interview (The Planet sacked him) by missing his train. Said train has The Parasite waiting on the tracks, and he drains them down to clothed skeletons, which are found at the next stop. Jimmy decides his luck may not be so bad after all. The cover to this issue has him running down the tracks as a skeletal train attendant bears the train down on him.
- A Post-Crisis arc has The Spectre dealing with a demon-ghost who snakes tentacles into a subway car that drink the blood of all they find.
- In the original comic of The Crow, Eric Draven is travelling to the afterlife in a train carriage before he remembers his murdered girlfriend, as he still has Unfinished Business in the world of the living. When Death asks him for his ticket, he turns back.
Films — Animation
- The catbus in My Neighbor Totoro.
- Spirited Away: The train Chihiro rides to get to Zeniba's home is intended for use by the dead moving on to the next life, driven by a faceless conductor and holding silent soul passengers (who are creepily represented as semi-featureless shadows). Kamaji comments that it "used to run both ways, but these days it's a one-way ride."
- The train station announcer in Wreck-It Ralph mentions a Soul Train departing from one of the outlets, but it's not clear whether this actually has anything to do with death or the afterlife.
- The entirety of Night on the Galactic Railroad, in which the main character, Giovanni, finds himself and his friend Campanella on a magical train journey across the night sky. At the end of the film it is revealed that Campanella had died saving another boy from drowning, and he has to journey on while Giovanni returns alone. The train scene from Spirited Away (mentioned above) seems to be a visual reference to this film.
Film - Live Action
- Woody Allen's Stardust Memories has one in the opening, simultaneously bleak and Played for Laughs.
- Ghostbusters II
Egon: Did you catch the number on the locomotive?Winston: Sorry, I missed it.
- Pirates of the Caribbean's iteration of the Flying Dutchman is the nautical equivalent. In the Caribbean. Captained by Scottish Cthulhu with a Welsh name.
- In Defending Your Life, all souls board trains following judgment to take them on to their next incarnation.
- In Dreamscape, the fight with the villain takes place in the President's nightmare on a train filled with dead-looking mutants traveling across a United States after a nuclear war.
- The film Heart and Souls features a variant involving a bus that comes (way behind schedule apparently) to collect the ghosts now that they've had time to finish their Unfinished Business. In fact, it is the same bus (and driver) from when the four of them were killed. After they explain that nobody ever told them why they were still around as ghosts, the driver agrees to give them some time to finish their affairs now that they know HOW to, but the bus returns periodically to pick them up one by one with no more negotiation or leeway.
- Played with in Inception. The train that is mentioned in the famous quote was actually used by the main character and his wife to return to reality after being trapped in a dream state. Not by boarding the train, though, but by deliberately letting it run them over, since the usual way of exiting a dream is by killing yourself.
- The Frighteners has an afterlife express, but it only goes to hell (those who go to heaven just sort of appear there). In a way, it looks more like a gigantic worm that swallows whoever it's transporting and then sticks pieces of its own skin into them to make sure they stay in place/torture them.
- The Mummy (1999) has a phantom chariot that comes for Imhotep's soul.
- Casper: A Spirited Beginning has the titular character find himself on one at the beginning. (He also doesn't realize he's dead at this point.)
- In The Heavenly Kid, Bobby dies and is immediately transported to a mysterious subway train taking him to the afterlife.
- In Darby O'Gill and the Little People, the Cóiste-bodhar (death coach) and its headless driver, a Gan Ceann, make an appearance.
- This trope is discussed in From Hell. Sergeant Godley is curious about the custom of placing coins on a dead person's eyes. Inspector Abberline explains that the coins are to pay the ferryman who takes people across a river to the land of the dead.
- In Here Comes Mr. Jordan it's a plane. In it's 1978 remake, Heaven Can Wait (1978), it's upgraded to a Concorde SST.
- Robert Bloch's Hugo Award-winning short story "That Hell-Bound Train".
- The train in the fifth The Dark Is Rising book, Silver on the Tree.
- In Maire Philips' Gods Behaving Badly Angel Islington Underground station is a gate to the Underworld of Greek mythology, with the dead taking Tube trains to the afterlife.
- One of the Choose Your Own Adventure books centered around this.
- The Dresden Files: In the conclusion of Changes, Harry Dresden, fatally injured and sinking into a lake, hears the sound of an oncoming train. In the very beginning of "Ghost Story", you learn that there IS a train coming for him, and he has to be dragged out of the way to stop it from hitting him. This is described as a "southbound train", one that presumably leads to Hell. The Big Bad of "Ghost Story" is finally brought down by a rush of spirits so direct and forceful that it is itself described as an oncoming train, and when said Big Bad screams in terror, Dresden says that her scream was drowned out by the "sound of a southbound train".
- In Graham McNeill's Warhammer 40,000 Ultramarines novel Dead Sky Black Sun, Uriel and Pasanius are dragged off a spaceship onto a Chaos-warped train that runs on the "blood-tracks" and carries them into a Chaos Space Marine capital world in the Eye of Terror.
- Sort of a Galaxy Express 666, then?
- In the third Marla Mason book, Dead Reign, by T.A. Pratt, Marla journeys to the Underworld via a train made of the thighbone of a leviathan. She takes this train from a subway station in San Francisco.
- One of Manly Wade Wellman's Silver John stories, "The Little Black Train", has the local Rich Bitch trying to escape a curse that the train will come for her (by removing all the local tracks). "A black train runs some nights at midnight, they say, and when it runs a sinner dies." It comes anyway, but she repents and the train retreats.
- Fire and Hemlock by Diana Wynne Jones has one of these.
- Harry Potter and the Deathly Hallows: The place that Harry visits in his Near-Death Experience turns out to be King's Cross Station, where he first went Down the Rabbit Hole. It is implied this is just what Harry sees, and that it takes a different form for different people. Dumbledore points out the significance of his imagining a train station, and that he has a choice to make between going back, or boarding a train and going "on".
- In the Star Trek novel I, Q, Q, Picard and Data ride a miles-long train of livestock cars filled with listless people. In this surreal story, what is actually going on is slightly unclear, but they know it will carry them to their doom.
- In The Rime of the Ancient Mariner, it's a ship, not a train, but Death and Life-in-Death play dice for the crew's souls.
- The Polar Express is a more benign form of the mysterious supernatural train.
- Played with in Neverwhere. An Underground train of this sort appears to Richard during the ordeal of the Black Friars; however, the catch is that getting on the train, rather than committing suicide on the tracks, is what allows him to LIVE.
- Christopher Fowler's novel Hell Train has some living people tricked onto boarding a demon train that takes damned souls to Hell.
- In Elsewhere by Gabrielle Zevin, Liz goes to Elsewhere (the afterlife) on a cruise ship.
- The Big Bad in the first Ghost of a Chance uses "Downbound" trains (which normally drag people to hell) to build itself a physical body.
- Stephen King 's short story "Mrs. Todd's Shortcut" appears to have this as the ending. The story is told from the POV of an elderly man. Mrs. Todd herself is crazy for shortcuts to strange places, and by the end, the narrator goes with her.
Live Action TV
- Tales from the Darkside: The second season episode "The Last Car" had five souls trapped for all eternity on an Afterlife Express with nothing but a box of sandwiches and eternal boredom to keep them busy. Whenever they go into a tunnel, they turn into skeletons and black out.
- In an episode of Seriously Weird, Harris and all of the customers of the diner find themselves trapped on a ghost train (the diner originally having been a car from this train) with a ghost conductor determined to drive them to their deaths and down to Hell.
- The third
and finalKamen Rider Den-O movie features a ghost train, hijacked by an evil Rider for his own purposes. The other trains in the series perform much the same function as Soul Trains except for time-lost individuals instead of dead ones.
- Star Trek: Voyager introduces the Barge of the Dead, from the episode of the same name, where the dishonored souls of Klingons are sent to Gre'thor, or hell.
- The first episode of Spielberg's Amazing Stories TV series featured an old man racked by guilt over killing everyone on a train in his youth. At the end of the episode, the train he'd derailed — full of the ghosts of the people who had died — pulls into the station, and the ghostly conductor leads the old man on board.
- The Twilight Zone (1959): "A Stop At Willoughby": A man is riding a commuter train home from work and it has an announced stop at Willoughby, and it looks like such a nice, quiet place like an 1800s public square with a gazebo, band concerts, picnics etc., but then finds there is no such stop on the railroad. He realizes that it's actually a portal to the afterlife, and decides to get off, discovering it's just as nice as he thought it would be. On the train, horrified passengers saw him get up, screaming "Willoughby!", then run and jump off the train. His body is shown being carried away in a hearse, and the rear door slams, noting the name of the undertaker: Willoughby and Sons.
- The Twilight Zone (2002): "Night Route" had a woman being stalked by a creepy-looking bus driver and his bus full of souls after she narrowly avoid being hit by a car. The Karmic Twist Ending here is that not only did the car actually hit her, but the bus driver isn't Death. He's Life, and he goes to people on the brink of death when it isn't their time yet and shuttles them off to the world of the living again. Sadly, she doesn't realize this until it is too late and vanishes in a wisp of smoke.
- Are You Afraid of the Dark?:
- The Tale of Station 109.1 revolves around an off-the-dial radio station that calls lost souls to the other side. The protagonist, who discovers the station on a hearse's radio, ends up being mistaken for a dead person and accidentally gets sent through the gate.
- A literal example is featured in The Tale of Train Magic, in which the ghost of a railroad conductor who died in a train wreck returns to look for a new conductor to take his place on said train.
- The gospel song "Plenty Good Room (On The Glory Train)" is a cheerful song about the express-train to the big rail station in the sky (which was considered by many to be preferable compared to the life of a slave in the early 19th Century American South). Trains to heaven are often found in gospel songs, among them "The Gospel Train (Get On Board, Children)" and Curtis Mayfield's 1965 "People Get Ready". The oldest train to heaven song may be the 1860s Scottish hymn "The Gospel Railroad".
- The Chuck Berry song "Downbound Train" features one of these headed to Hell and driven by Satan himself, whose passengers have all lived lives of vice.
- Not to be confused with the Bruce Springsteen song of the same name, where the singer has had terrible luck and just feels like he's on one of these.
- But his anthem/hymn "Land of Hope and Dreams" is a glorious takeoff on "Plenty of Room on the Glory Train". While that song warns that only those who "mind what they say and do" will be allowed, Springsteen's lyrics emphasize a Christian-like forgiveness of the sin, shame and loss often experienced by his working-class protagonists.
- The Chris de Burgh song "Spanish Train" is a story about a train carrying the souls of the dead to the Underworld. God and the Devil are playing Poker - gambling with the souls. Naturally, the Devil cheats and wins the game.
- At the end of the song, they've moved on to chess. And the Devil is still cheating.
- The Hank Snow song "Ghost Trains" is about a normal person watching two of these racing each other. The drivers seem fairly happy for eternal railroad ghosts, though.
- The Vernian Process song "The Last Express" centers around this trope:
Will it go on forever?No one will ever see.As it rolls along in silenceFor all eternity!
- Iron Maiden has, besides "Rime of the Ancient Mariner" (a Filk Song based on the poem mentioned above), "Ghost of the Navigator", where a sailor meets during his final journey a ship filled with ghosts (though Word of God states that they're "ghosts of his subconscious").
- Not sure if this should go under Music or Western Animation, but the "Blues" song from the defictionalized band Dethklok from the show Metalocalypse has a song "Murdertrain a Comin' (Why is this song the Blues? Because it's about a train, of course!). A sample of the lyrics:
Here comes the soul collecting train of murder a-comin'
It longs to take your putrid blackened soul away from you
Your face will leave your rotting head, in the early morning
Your guts will leave your corpse, your spine will break and crack in two
- The bluegrass song "Little Black Train" and the more recent "Long Black Train" are both about this.
- "Penn Station" by The Felice Brothers has a newly dead protagonist waiting to see which of two rival Soul Trains, from Heaven and the... other place will get to him first.
- "City of New Orleans" puts a spin on this. In this song, it's the trains themselves that are headed to the proverbial afterlife, not their passengers. Steve Goodman wrote the nostalgic song about air travel driving passenger rail service out of business:
All the towns and people seem
To fade into a bad dream
And the steel rails still ain't heard the news.
The conductor sings his song again,
The passengers will please refrain
This train's got the disappearing railroad blues.
- Amtrak actually still operates a passenger train called the City of New Orleans, but the rest of the song's predictions are spot on. Effective passenger rail service has almost completely disappeared from the USA.
- Johnny Cash's "Redemption Day":
There's a train that's heading straight to heaven's gate, to heaven's gateAnd on the way, child, man, and woman wait, watch and wait, for Redemption Day
- "Keep Me In Your Heart For A While" by Warren Zevon uses boarding a train as a metaphor for Zevon's own terminal illness:
''Engine driver's headed north to Pleasant StreamKeep me in your heart for a whileThese wheels keep turning but they're running out of steamKeep me in your heart for a while''
- Patty Griffin's "Stay on the Ride" is about a nameless little old man who takes a bus ride to a destination never named, though the description makes it sound like someplace after-life-y. Despite the title, he gets off before it reaches it.
- One theory on how the American folk song "Wabash Cannonball" originated is that 19th century hobos imagined a mythical train that would collect the souls of unemployed drifters who'd died while riding the rails.
- On "The Lamb Lies Down on Broadway" by Genesis, the protagonist is trapped under a rockslide, awaiting death:
Anyway, they say she comes on a pale horse, But I'm sure I hear a train.
- Lemont Brown of Candorville sometimes dreams that he boards a train from Earth to outer space, where he converses with recently deceased celebrities. For instance, he spoke with Steve Jobs in 2011 and shooting victim Trayvon Martin in April of 2012.
- Angus Og: At one point Angus, Tonald, and Mairileen hitch a ride on what appears to be a Clyde Puffer called The Flying Teuchter which turns out to be crewed by demons from hell, and owned by Old Nick himself.
- Railroad folklore says you should never mimic the sounds of a train whilst on the railway tracks, especially at night, lest you summon said Soul/Ghost Train.
- Silverpilen, an unpainted subway test train from Stockholm once used sporadically as a backup unit, is subject to legends of this kind.
- The United States has many Ghost Trains but the most famous is the one that has been carrying Lincoln's coffin back to Illinois for a hundred and fifty odd years now. 
- Wraith: The Oblivion features the Midnight Express, a train traveling through the Shadowlands that serves as neutral ground for all the rival factions of the afterlife — including those serving Oblivion.
- Exalted: The Midnight Express also shows as a mysterious soulsteel and moonsilver train that travels through the Labyrinth, conveying passengers to and from the Mouth of the Void. The Deathlords are interested in taking control of it; to date, they haven't succeeded.
- Call of Cthulhu supplement Fearful Passages, adventure "Iron Ghost". "The Train That Ever Was" carries its victims to a terrible fate: to be devoured by Azathoth.
- The Black Engine in Deathwatch campaign The Emperor Protects. It's a daemonic entity in the form of a train, which follows rails designed to allow it to become incorporeal and exist outside real-space. In exchange for its services, transporting psychic individuals so their souls can be drained, it's fed human slaves on every round-trip.
- The Tour Bus From the Underworld from Yu-Gi-Oh!.
- There's also the Tour Bus to Forbidden Realms; judging from this card and Shared Ride, the "Forbidden Realms" is a place that monsters have to go when their cards are put on the Forbidden List, courtesy of this bus. Sangan ends up boarding this bus by Mistake, and is horrified. In Shared Ride, Sangan has a Freak Out and starts crying, but Graceful Charity comforts him.
- In the three versions of Hell Rail, the player' task is building railroad tracks and sending damned souls to their respective circles in hell.
- In Guys and Dolls, the song "Sit Down, You're Rocking The Boat" is about a dream the singer had about sailing away on the boat to Heaven.
- The play The Ghost Train would suggest this trope... but it's actually a mystery thriller in the vein of Scooby-Doo decades before said cartoon was invented.
- One of the sets in the LEGO Monster Fighters series is a flying ghost train driven by a trio of ghosts.
- Kingdom Hearts II: One of The Seven Mysteries of Twilight Town.
- It turns out to be a magic train that carries people to the Mysterious Tower, home of Yen Sid.
- The Legend of Zelda:
- A ghost ship in The Legend of Zelda: The Wind Waker and The Legend of Zelda: Phantom Hourglass. Overlaps with Flying Dutchman.
- Ocarina of Time: The Shadow Temple ship (that floats on shadowy mist!). It's not like there aren't ample death metaphors elsewhere in that place...
- The Legend of Zelda: Spirit Tracks: A very weird exception appears: There's the Cool Train and a Ghost rides it (along with a living passenger), but the ghost in question gleefully giggles, smiles and plays around on the train while it drives around, removing all the creepy from the situation and moving it into Crowning Moment of Heartwarming territory instead. The commercial plays it a bit more straight.
- Final Fantasy VI: After wandering onboard such a train, you have to fight it (of course) to get off. Since it's undead, a very common item will easily destroy the boss for you (or you can have Sabin suplex the train). One creepy detail about the train is that you can look at the schedule book, only to find that it's blank. It happens that the war going on is causing so many deaths that the train is pretty much running nonstop, and has been for some time.
- The same train also appears as a Bonus Boss in the GBA remake of Final Fantasy I. The summon ("Doomtrain") in Final Fantasy VIII is also a reference.
- The Final Fantasy Phantom Train is used as a base for the Doom Train level in Super Mario Fusion Revival.
- In Final Fantasy Tactics, one of the wonders you can discover is a soul train. Likely a direct reference to the aforementioned Phantom Train from Final Fantasy VI, seeing how many of the other wonders in the game happen to be locations and objects from the previous games in the series.
- In Sonic Adventure 2, there are a few soul trains in the Pumpkin Hill level that keep circling through walls, though they don't really serve any purpose. Except running Knuckles over whenever the player isn't paying attention.
- In Grim Fandango, the Number Nine express train carries only the most saintly of souls to the Ninth Underworld in four minutes instead of four years like the others who have to travel by different means. Illegally obtaining your ticket, however, has dire consequences, as the entire train quite literally goes to Hell as a result.
- Darkstalkers 3 had a stage called Iron Horse, Iron Terror set on an apparently sentient train engine complete with an eyeball headlight and several twitching mouths, driven by a skeletal conductor. For a series based upon horror tropes and everything related to the lore surrounding it, it's rather unsurprising that a Soul Train would be in it.
- There's one in Skies of Arcadia too. Travels at high altitudes between Nasr, the Lands of Ice, and Mid-Ocean in a triangular route.
- Sam & Max: Freelance Police: Sam and Max board one to visit Hell, LLC.
- Metro 2033 has one in the level "Ghosts". The headlights are visible as it heads down the rails, but the train itself (and if you look in the windows, the passengers) can only be seen as a shadow via your flashlight.
- Played with in Alice: Madness Returns where The Infernal Train that has replaced the old Looking Glass-line, and is polluting and destroying Wonderland as it goes, doesn't represent a physical death, but rather a mental one.
- Indie visual novel Train Of Afterlife is entirely about... well, you can probably guess.
- Inverted in Afterlife by LucasArts, in which trains are used to transport reincarnating SOULs back to the planet.
- Hatoful Boyfriend has several references to Night on the Galactic Railroad with regard to Nageki. It's his favorite story, and Anghel's fantasy-counterpart-nickname for him is "Estelle Campanella" (Campanella of the Stars).
- In the sequel Holiday Star, the bird afterlife actually is traveling the universe on the Galactic Railroad until you forget your past life and are reincarnated. Judging by the human girl's familiarity with the Conductor, this is also her fate after all those bad ends.
- Gloria, the train in the Charnel House Trilogy, is one of these. It's made obvious to the player at the end of the first episode, but the characters who board take a bit longer to catch on. The fact that the train seems like an apparently normal, if antique, train for the longest time only makes it more unnerving.
- Lucidity. The entire game is about Sofi reconciling the death of her Grandmother in a dream world. The game ends with Sofi getting to say one final goodbye before her Grandmother gets on the Afterlife Express. She wakes up soon after.
- The Tom and Jerry short "Heavenly Puss" features one. Tom has to go back to Earth and make peace with Jerry before they'll let him aboard. He doesn't make the train in time and ends up literally being dropped into Fire and Brimstone Hell, but luckily for Tom it was All Just a Dream.
- An episode of Hey Arnold! had Arnold, Helga and Gerald investigating an urban legend about one. The supposed train ends up arriving, and the trio get aboard... It turns out to be a train to a metalworking factory, and there is no ghost train after all... or is there?
- On the Spongebob Squarepants episode "Graveyard Shift", Squidward tells SpongeBob the story of the Hash-Slinging Slasher, who on every - what day is it? - Tuesday night comes to the Krusty Krab on the ghost of the bus that ran him over. Later that night, a bus arrives in a cloud of fog.
Spongebob: I didn't know the buses ran this late.Squidward: They don't!
- Long John Scarechrome's ship in Filmation's Ghostbusters.
- In The Real Ghostbusters episode "Knock Knock", the Ghostbusters board a train in New York, only to realise that it has been taken over by demonic forces, its passengers killed and turned into skeletons. Their solution? Bust them all!
- Thomas the Tank Engine has both Percy and Peter Sam tell stories about ghost trains. Percy's story (and the accompanying cinematic sequence) is pure horror.
- The Replacements features Splatter Train, an in-universe B-Movie about a ghostly train who murders teenagers at Make-Out Point.
- In Futurama, Bender ends up on a roller coaster to Hell... Robot Hell... Complete with Singing Robot Satan...
- In the South Park episode "Dead Celebrities" recently deceased celebrities (Farrah Fawcett, Billy Mays, David Carradine, Ed Mc Mahaon and and DJ AM") are stuck waiting on a plane to the afterlife that has yet to take off because Michael Jackson has not yet shown up.
- On Friday, August 27, 2010 a group of people gathered at the site of an 1891 train crash to see if they could hear the purported Ghost Train. Instead they ended up finding the Railroad Tracks of Doom.
- This trope is possibly a modern form of the Totentanz image updated to modern civilization. However, the Afterlife Express rarely includes singing and dancing.
- Taken almost literally (well, it takes one to their grave but there the railway company's responsibility ends) with funeral trains; even Welsh industrial tramways such as the Ffestiniog had hearse vans. Some notable examples include the London Necropolis Railway; Rookwood in Sydney, New South Wales; Spring Vale in Melbourne, Victoria; and the very short-lived New Southgate Cemetery in London. These were a neccessity in Victorian times as the great necropoli were being built well outside the cities they served; in the case of Rookwood the necropolis was at Haslems Creek, which in the 1860s was a couple of days' travel for a horse-drawn funeral procession. The early 20th century saw these losing their admittedly disappointingly-low business to motor traffic; the Second World War saw a brief spike due to fuel rationing, but almost as soon as the war ended (London Necropolis having been rendered unworkable due to damage during the war) they were to follow their clients into history. The line to a certain graveyard in Newcastle, NSW was still open until the 1980s, as it was near a commonly-used stop, but hadn't carried any bodies for some years. The railway put on special weekend trains for relatives to go and maintain and decorate the graves, which were always packed on Mothers Day. In Melbourne, there was reportedly a special funeral tram which picked up bodies and took them to the train. Now little is left of most of these, but for the Mortuary station in Sydney, and the terminal at Rookwood, which was purchased and dissembled in the 1950s and was rebuilt as a church in Canberra, ACT.