The original anime also gives him a set of white glowing eyes when his Battle Aura kicks in and his shirt comes off.
The title character of Hayate the Combat Butler resorts to this after being hit by a car. The occupants instantly surrender. It gets used a little more in the manga than the anime, but mostly by Hayate and it's pretty much always a Crowning Moment Of Awesomesuch as coming back from a Curb-Stomp Battle against Machina because the latter tried to take the Stone of Bonds or Crowning Moment of Funny such as when Hayate actually does something nice for Kotetsu and promptly takes him out with a vicious elbow to the chest when he tries to propose again.
Quant and Yuri from Tower of God sometimes sport red glowing eyes in the dark. They stem from a technique that enables them to see in the dark. Which coincidentally is when they are mist willing to grievously injure people.
Zeon mecha from Mobile Suit Gundam in particular are notable for a single mono-eye that seems to have no purpose other than to swivel towards the camera and glow menacingly.
Gundam Wing took it a step further, with the image of a Gundam or other mobile suit appearing in front of an enemy bridge, eyes glowing, before the bridge explodes (usually due to a beam saber strike) becoming so visually impressive that every series since has copied it.
In Gundam00, when the innovators use quantum brainwaves to send messages to each other. Or, alternatively, when "fucking Ribbons" uses said quantum brainwaves to hijack their minds.
Also, the Evas' eyes in Neon Genesis Evangelion glow white when they enter 'Berserk Mode', which generally means their opponent is doomed. Rebuild of Evangelion takes this a step further at the end of the second movie when Shinji, his EVA out of power and the 14th angel beating the hell out of him, finally snaps. We are treated to an absolutely terrifying scene of his eyes glowing Blood Red before, in an explosion of pure rage, he re-activates EVA-01 through sheer force of will and begins one of the most awesome beat-downs in the franchise's history. If there is any possibility for his Eva to look even scarier than it normally is while berserk, this is it.
When Mari switched Unit-02 into Beast mode, her eyes turned into an almost white hue of green. Asuka later gets glowing blue eyes when she puts Unit-02 into Beast mode, even her left eye (under the eye-patch) glows.
In conclusion, it seems that taking the Entry Plug to unsafe depths causes this trope as a side effect. It may have something to do with all those warnings that a pilot who does this risks losing their humanity.
Tengen Toppa Gurren Lagann's title mech, the Gurren Lagann, does it as well, usually combining it with something lighting on fire as the heroes become even more resolved. The one time this is used against them is phenomenally awesome, as the main bad guy of the first part of the series gets out of his Ganmen, walks over it, on foot, with his head on fire, with glowing eyes of doom raging, and PUNCHES THE SHIT OUT OF LAGANN BARE-HANDED. Lord Genome is a force to be feared.
Most of the cast of Higurashi no Naku Koro ni do this both humorously and seriously. Not being able to tell which is which amps up the creep factor.
Although it still isn't common, a lot of characters exhibit this trait — including both Elrics, Izumi, Winry, most (if not all) homunculi and some others.
On a more humorous note, Ed's eyes occasionally do it too when someone insinuates that he's short.
Roy did it (hilariously) when Barry the Chopper was hitting on Hawkeye. He pulls a much less hilarious and much more terrifying version of this when completely curbstomping Envy, especially when the latter tried to kill Riza.
In Brotherhood, Kimblee once does this while giving Ed a Breaking Speechand then proceeds to blow him up with a hidden Philosopher's stone.
Averted with Al's eyes which appear as small pools of light, that disappear when he is unconscious.
Azumanga Daioh's Yukari-sensei, after Tomo accidentally hits her in the face with a tray.
Happens often in the first half of Dragon Ball Z, most often pertaining to Gohan, who would occasionally fall into a superpowered psychotic rage, during which his eyes would lack pupils and become more pronounced, at least during the loss of control.
Goku also does this during his first Super Saiyan transformation.
Happens later in Dragon Ball GT with any characters possessed by Baby.
When Future Trunks powers up to Ultra Super Saiyan against Perfect Cell, his eyes briefly flash and glow white, similar to Broly in his Legendary Super Saiyan form.
Yusuke from YuYu Hakusho gets these several times; once during his fight with Suzaku when he's about to use up all his life energy to win, once while glaring at Younger Toguro from the stands of the Dark Tournament, and occasionally thereafter while powering up or in the middle of a particularly intense fight - especially when, at the end of the Chapter Black saga, he is taken over by his ancestor, Raizen, shortly after being killed and returning to life as a demon. Where before Yusuke's Glowing Eyes Of Doom have always been blue, these are red, possibly to signify either his new demonic nature or the foreign presence in his body.
Also Sensui when he releases his sacred energy does it in an eerie and gradual way, followed by a powerful laugh that literally stresses the planet as he powers up.
Fate did this in the first season of the show during her second appearance, appearing as a black silhouette with Alucard-style glowing yellow circles.
Ryoko in Tenchi Muyo! does this sometimes. It's not too big a deal if they're glowing yellow — that just means she's a bit narked — but when they glow red, she's ready to kick booty at the planetary level. If they turn green, well, let's just say she's not being herself.
Parodied in Magical Project S when Ginji Kawai (Sasami's father) gets a little over-enthusiastic while flying Washu's rocket.
When informed of the fact that she could turn Genshiken into a cosplay research club as president, Ohno gets Glowing Eyes Of Doom. In fact she gets Glowing Eyes Of Doom whenever she gets sufficiently obsessed about something, mainly cosplay-related.
Two characters from Mai HiME get these — Mikoto (gold) and Nao (red). Also Shizuru, when she arrives to help Natsuki against Nao, knocking her and her Child off a cliff (although she still appears to be sane at the time).
When Chika finds a photograph of Tina and Kaoru In Ai Yori Aoshi, Tina gets perfectly round Glowing Eyes Of Doom combined with Hidden Eyes-style shadowing. After the cutaway for various spooky imagery, Tina's bright and cheery, while Chika'spetrified.
The most powerful fighters in Ikki Tousen get these when they summon their inner dragons.
Ichika from Uta Kata gets glowing eyes when she gets afflicted by her powers, leading one very spooky scene when she approaches Manatsu while she sleeps.
Zelgadis in Slayers gets these when Rezo takes control over him when he needs to stall for time. A few scenes later and Rezo manages to summon Shabranigdo — only to learn that Shabranigdo was actually sealed inside his own eyes. Naturally, this leads to more glowing eyes to signify that Shabranigdo is taking over Rezo's body.
Death the Kid also starts glowing toward the end of the anime.
Might be the intended look of how Noah's eyes are occasionally drawn in the manga.
Members of the Star Clan gain golden star pupils.
Maka only has flat green eyes normally, without any pupils, but when she is angry, scared, or in a fight, her eyes glow yellowish-green and pupils appear. Interestingly, if she is shocked they glow red for a moment, but only in the anime.
Lime in Saber Marionette J has these when first activated, and again when Hanagata pisses her off.
The eyes of Tohno Shiki and Ryougi Shiki from the Nasuverse glows bright blue; it is the result of their Uh-Oh Eyes of death. Ryougi's eyes only does this when she activates them but Tohno's eyes are always activated. They become more apparent when he takes off his glasses, though, a sign that he is in his "killing mode."
Creed from Black Cat did this when his precious time with Train was constantly interrupted by others. And no, it wasn't played for humor.
Rail Tracer in Baccano! enjoys this trope almost as much as it enjoys dragging people under moving trains and grating their limbs and faces against the rails, which is a lot.
Black Lagoon managed to pull this off in a late episode when Rock upset Balalaika - she stood over him at an angle at which light reflected off her glass eye, evoking the trope.
Both Alucard and Anderson invoke this trope whenever they stop screwing around and are about to curbstomp someone into the ground.
Seras Victoria gets it later on when she becomes a true vampire and regenerates her eyes. The effect is enough to terrify a horde of Nazi vampires. They're right to be frightened, considering what she does to them next.
In Berserk, the houndlike "Beast" that sometimes appears in Guts's dreams and that represents his darker side post-Eclipse has two really evil-looking red glowing eyes, and when Guts is under its sway, his good eye will often glow red like the hound, best illustrated in the PS2 game which focuses on the Millennium Falcon arc.
In the Chrono Crusade anime, the demons tend to have Glowing Eyes Of Doom when they're unleashing their full powers. Chrono in particular seems to get these when he's being overcome with Unstoppable Rage.
Pokémon: If Mewtwo does this and he's looking at you, kiss your ass goodbye. Same goes for Psyduck.
While not particularly malevolent, the Bus Driver and school Chairman both have glowing, white eyes in Rosario + Vampire.
Towards the end of the Tower of Heaven arc in Fairy Tail, Natsu eats some of the Etherion Initially his eyes glow ice blue, the same color as the Etherion; then he starts choking, and his eyes blaze up into flames. Much ass kickery ensues.
The eponymous Claymores get this when they activate their Yoma powers.
Vincent's eyes start to glow when he becomes Ergo Proxy. This generally does not bode well, considering what he is. Similarly, the other Proxies also exhibit this trope when initiating aggressive action.
Compared to most of the other entries on this page, the settings and characters in Detective Conan are realistic. However, during one case set at a hotel's onsen, Conan inadvertently mortifies Ai several times while delivering the case's resolution. Ai not only gains this trope, but also a malevolent aura on top of that.
When a villain (or Wolverine) is really getting on Cyclops' nerves, a glow can be seen through/around his shades or visor. As his Eye Beams are always on, this probably indicates that their blast is becoming far more intense.
Storm's eyes become solid white and glowing when she uses her powers. This carries into almost all adaptations.
When Magneto gets angry, his eyes glow as well, independent of power usage.
Gambit's eyes have been known to give off red energy when he's intended to look particularly badass.
Cable and his alternate-universe clone X-Man both have glowing left eyes.
Only when Liefeld manages to remember that the left eye is the glowing eye, since he seems to forget half the time.
Being related to Cyclops will do that. His other brother Kid Vulcan has them, and his alt-reality daughter Ruby does as well.
Sometimes Nightcrawler is shown with Glowy-eyes when he's lurking in the shadows.
Jean Grey and anyone else possessed by the Phoenix Force tends to have solid glowing gold eyes when they use the Phoenix powers.
Her daughter Rachel (who actually was the Phoenix host for far longer Jean, not that any writers remember that these days) has a glowing left eye (just like her half-brother Cable; see above), except that in addition to the glow it's surrounded by a glowing Phoenix emblem. Normally it's yellow, but a recent upgrade turned it blue.
When Wolverine is introduced in the first Ultimate X-Men storyarc, he is seen perched up in a tree, hidden in the shadows. Except his eyes are glowing for some reason. Not "cat-eye reflected in the light" glowing either, more like "Superman about to unleash the heat vision." No explanation given.
X-23's eyes normally just turn red when she's under the effects of the trigger scent, an indication that she's about to turn anyone who gets in her way into meat confetti. However in one arc of her solo series they actually started glowing.
In the Star Fox comic from Nintendo Power, Fox McCloud gets these on two occasions. One is right at the beginning, where Falco hits Fox's Berserk Button by suggesting raiding commercial freighters instead of the Imperial ships the team usually targeted in the beginning ("Feathers for brains! For the last time, we are NOT THIEVES!"). The other is right at the end when Fox goes into Unstoppable Rage mode after learning the truth about what happened to his mother and father.
Even Badass Normals seem to get glowing eyes when they're lurking in shadows. The entire Bat-family for example seemed to have glow-in-the-dark lenses in their masks. Rule of Cool excuses this for the most part.
Sistah Spooky from Empowered seems to get these under extreme emotional stress. How extreme? Picture watching the love of your life die as you are literally seconds from saving her, then having your worst enemy (in your own mind) show up to gloat. If things get that bad, running is not even an option. Said 'enemy' breaking down because she chose to save her over herself, is.
Due to their connection with the Speed Force, Flashes and other speedsters sometimes get glowing eyes (or, as Bart likes to put it, "lightning eyes"). This is mostly involuntary, but it usually does happen at moments clearly meant to be significant, so it all works out.
In Mega Man, the end of issue 3 had the Robot Masters standing behind Dr. Wily with their eyes glowing.
Doctor Strange sometimes gets these when spellcasting. Considering that he has a lot of dangerous spells which don't make his eyes glow, these are a good cue that he's really getting serious.
Similary to the above example, Wiccan of Young Avengers has these when using his spellcasting/reality warping abilities; but the most memorable example is when he put everyone in Central Park into a magical coma.
The dwarven noble protagonist, being a Spirit Warrior of sorts, suffers from this effect whenever he calls on the energies of the Fade. The glow is pure white and can extend to his whole body and even become something like a shroud of mist-like gossamer strands around him, depending on how much power he pumps out.
Alim Surana has a true sight spell that makes his own eyes glow blue. His irises can shine crimson too, when he uses blood magic.
In Game Theory, the automatons in the Garden of Time acquire these when the Jewel Seed tied into the defense system activates.
In The Iron Giant, the title character's eyes glow red whenever he sees a weapon or is fired upon. If his rage becomes too great, he transforms into a nigh-unstoppable killing machine capable of taking out tanks (and possibly battleships).
Chernobog, the Slavic dark god, in the "Night on Bald Mountain" segment of Fantasia.
The Secret of NIMH has a more benign application of this. Both Nicodemus and the Great Owl have glowing eyes to indicate that they are beings of mystical wisdom. And if you've never read the book, you'd be forgiven if you assumed that implied they were the same being (what with the glowing eyes and wispy grey fu manchus...)
Megamind: Titan's eyes tend to glow when he get's really pissed off.
Aladdin: Jafar in his genie form has glowing yellow eyes, visible for about two seconds before he gets stuffed in a lamp.
In the film adaptation of Dune, the entire Freman race has glowing blue eyes, as a side-effect of their addiction to the Spice. In the original novels, however, it was simply altered pigmentation, and they're eyes were just a weird kind of blue.
An interesting variation occurs in the Lone WolfgamebookThe Kingdoms of Terror. Lone Wolf is being attacked by a very fast monster with glowing eyes in a pitch-black castle. If you choose to fire an arrow at its eyes you actually get a bonus to your success roll since, because they're glowing, they're an easier target.
Is an reccuring theme in Phenomena, Tarkan has red glowing ones, Ezau has white glowing ones, and Azur has them, although he's not one of the antonagists.
Drool Rockworm's permanently red-glowing eyes in The Chronicles of Thomas Covenant are a typical example of this. On the other hand, Caerroil Wildwood with his silver-glowing eyes is not, being neutral to the good guys and doom for the bad guys.
Lord Foul's eyes are definitely this, especially since they're the only things that are in focus.
The yellow glowing eyes is common to all Dark Side practitioners in the Star Wars universe, and usually comes as a result of deep immersion in the Dark Side of the Force. Golden-yellow irises with a bloodshot rim, they are often referred to as "Sith Eyes" due to their obvious association with the most illustrious of Dark Side sects. Some Sith are even in this state permanently (Darth Maul, Darth Sidious after revealing himself.)
Grand Admiral Thrawn from Timothy Zahn's Star Wars spinoff novels is a Chiss, a race of aliens closely related to humans but with blue skin and featureless glowing red eyes. He's a gentlemanmilitarycommander of a villain, rather than someone who will flip out and burn you with laser beams from his eyes, and while he's definitely a bad guy he's complex and not strictly evil. The glow is nonetheless unsettling, and goes out when he dies.
Other Chiss show up elsewhere in the EU. While the most antagonistic any of the named ones has been was around the level of an Obstructive Bureaucrat, they all have eyes like that, and they all tend to have a little of Thrawn's inscrutability.
Flim simulates the glow with "self-powered surface inserts".
Subverted in Discworld with Death, who has glowing blueeyelights (unless he's angry) and is a lovable example of The Spock. It should be pointed out that the blue eyes in question are STARS and that in moments of "emotional" stress (Death can't really do emotions; no glands) they go supernova. Yes, you heard me, his eyes explode when he's angry.
Also subverted, and inverted, with the golems. They all have red glowing eyes as a byproduct of the magic that keeps them alive, but are innately harmless, and indeed most of them are prohibited from acts of violence even in their own self-defense. However, because of their implacable work ethic, near-human appearance, and glowing eyes, people tend to be creeped out by them, and unprovoked attacks of humans on golems are not uncommon.
Ba'alzamon from The Wheel of Time has eyes that occasionally appear to be pits of fire. After he's defeated, his body has its eye sockets burned out.
In Fiona Patton's Tales of the Branion Realm novels, royals have literally flaming eyes. In one book a four-year-old sovereign stares so hard at one of her vassals as she argues with him that her eyes grow hot and a "red-hot nimbus of flame" rises up between them. The man, old enough to be her grandfather, hastily submits to her will.
Sorcerers in Second Apocalypse have very bright glowing eyes (and mouth) when they sing their spells. In cases where extreme power is invoked this extends to a whole-body glow.
In the Doctor Who tie-in novel Forever Autumn, the aliens attack the Doctor & co. using possessed cats, and later, children whose eyes glow green.
In The Island of Doctor Moreau, the first definite sign Prendick sees that M'ling isn't just an ugly human is when he sees the beastman's eyes flash in the darkness, reflecting light like an animal's.
The Dresden Files: White Court Vampires have this as a gauge of how far into their "super vamp" mode they've gone. If they're normal, you're looking at a dangerous predator. If they're all white, back away slowly and then get out of dodge. If they're silver, makeyourtime.
Denarians also exhibit glowing eyes — specifically, the Fallen themselves will manifest eyes (usually green) when they are startled, angry, or in combat. They're not required to be in the same places as their hosts' eyes (which, due to Voluntary Shapeshifting, can be whatever shape, color, or number the host wants), and are usually on the forehead. As for the "of Doom" part, well, start with a Fallen Angel and go from there.
When Bob is awake, his skull sanctum's orbital sockets develop glowing orange sparks for "eyes". Normally they're not this trope, but when they turn blue in Dead Beat, it means his very scary hidden evil side has taken control. In Ghost Story, his Evil Bob side has become an independent entity with creepy blue eye-sparks.
In A Song of Ice and Fire the wights, corpses on the control of The Others, are described to have piercingly blue eyes, in this vein.
Beka Cooper, the protagonist of Provost's Dog. She doesn't have any evil side or Demonic Possession, just Icy Blue Eyes that get really intense when she's angry. People who see her like this assume she's a mage laying a curse, a fear she uses to her advantage on a couple of occasions.
Both the good and bad guys of The Stormlight Archive have them. Justified in the case of the heroic Knights Radiant, whose eyes glow when they've inhaled a bunch of Stormlight and are about to annihilate some evil. On the bad side, Words Of Radiance reveals that Parshendi eyes glow red when they become Voidbringers.
JC Chance of the Ghost Finders novels acquires brilliantly-shining eyes from the mysterious Light after his near-death experience in the London Underground. Unlike most heroic examples, it's not temporary or limited to battle, so he has to wear heavy dark sunglasses to go out in public.
The Goa'uld do this for intimidation, as well as involuntarily when taking on a new host or dying. Slightly subverted in the fact that the Tok'ra, the good alien cousins of the Goa'uld, also show off their glowing eyes. But they usually nod before, and that's an indication that the symbiont is going to talk, not the host.
The Doci in seasons 9-10 does this when possessed by the Ori, as does Adria on her own, both before and after ascending.
Lyta on Babylon 5 has both a Glowing Eyes Of Doom mode when under Vorlon influence and a glossy black eyes mode when resisting (or influenced by) the Shadows.
When Willow does some serious white magic in the last episode, she gets a pair of completely white glowing eyes.
Buffy gets a pair in "Primeval" as a result of the enjoining spell.
Carnivŕle: Ben Hawkins sometimes has glowing white eyes when doing something seriously holy.
Heroes: Isaac and Peter along with Sylar — once he steals Isaac's power — and later Matt, for no apparent reason, get glowy eyes when their in their precognitive trances. Ted, the Radioactive Man, gets glowy eyes when he's about to explode.
In Scrubs, when The Janitor makes the grave mistake of saying a new attractive latina nurse looks like a "young Carla", Carla gets glowy eyes and conjures up a powerful wind that blows all throughout the hospital (in an obvious Shout-Out to Storm) and emits a high-pitched scream that breaks Dr. Kelso's glasses and shatters Turk into a million pieces. ("So that's why they call him Glassman!") This all happens in an Imagine Spot by the way.
In the Star Trek: The Original Series episode "Where No Man Has Gone Before", Gary Mitchell is zapped by the galactic barrier, his eyes start to glow, and his psi powers grow exponentially. He goes power-mad and murders a crewmate. In the end, Kirk is forced to kill him.
The Master in the Made-for-TV Movie had constantly glowing green, cat-like eyes which he concealed behind sunglasses and used to instantly hyptonise Chang. The eyes are a continuity nod to the final story of the original series, "Survival", in which the Master was infected and under the influence of the Cheetah Planet that gave his last incarnation Supernatural Gold Eyes.
Another example would be Rose when she absorbs the Time Vortex energies. When the Doctor takes it out of her, his eyes glow too.
Ditto Donna when her Time Lord metacrisis brain kicks in.
In the episode "42", those who become possessed by the sentient sun — including the Doctor — have burning orange eyes.
The original Megazord, the Mega Dragonzord, and the Thunder Megazord would have their eyes flash while performing their Finishing Moves.
In an episode of How I Met Your Mother entitled "Slapsgiving 2: Revenge of the Slap", we see Lily's "you're dead to me" look, which is kind of an overly dramatic Death Glare. The camera zooms in on her face, her hair starts swirling around, she starts breathing intensely, and her eyes light up with a fiery glowing.
Johnny B. of Misfits of Science developed glowing eyes when his electricity-based powers were fully charged up.
Battlestar Galactica (Classic)'s robotic Cylons have one glowing red eye that slide back and forth with a scary metallic noise. Also, in some posters/still images for the Reimagined Series, the humanoid Cylons sport glowing red eyes as well, though this isn't in the actual show.
Angels from Supernatural would occasionally have glowing eyes especially when using their power. The big example would be Sam whose eyes would glow whenever Gadreel would take over.
The main villain on Thunderbirds, known as The Hood, has eyes that glow yellow when he uses his mesmeric powers.
In the original Vampire: The Masquerade, the single-dot Protean power was "Eyes of the Beast" or "Gleam of Red Eyes" depending on the edition. Aside from making the vampire in question's eyes glow red, which was great for intimidating some characters, a vampire using it could see in deepest night as though it was a clear day at noon.
Seemingly everyone in Warhammer 40,000 has these. It can only be assumed that any face-concealing helmet will have LEDs in the eyepieces. That said, the Salamanders chapter of the Adeptus Astartes actually DO have them: glowing red orbs that emit heat, with no pupil or iris.
Tieflings in the 4th Edition. Technically, they don't actually glow, but the fact that they're always a solid, often metallic, color seems to create a similar effect. For bonus points, they're often gold or red.
In 3rd Edition Dungeons & Dragons, psionic characters would gain glowing eyes whenever they used many of their powers.
Certain spells such as "Arcane Sight" make a person's eyes glow blue, and Darkvision can make a character's eyes flash or glow red.
First Edition's Oriental Adventures also has the "Fiery Eyes" spell, which makes the caster's eyes glow with an unnatural fire.
In the Palladium Fantasy RPG, a character could be "cursed" with glowing red eyes, which actually give him increased stats for the purposes of intimidation.
In the Warcraft RPG, at least in the first one, night elves (and perhaps high elves also; don't have now the sourcebook) are described to have no pupils and glowing eyes, especially under the moonlight.
You can give yourself custom cybereyes in Shadowrun with this effect.
Urban Legends and Myths
The West Virginia crypid Mothman's eyes are legendarily red, and often the only thing the witness will see (and sometimes be hypnotized by).
In Warcraft III, Grom Hellscream had glowing red eyes, caused by his embracing demon rage with open arms. The glow dies away a moment before his death because he killed the demon who caused the rage. In the expansion Frozen Throne, Arthas gets glowing blue eyes after fusing with the Lich King Ner'Zhul.
The Warlock class's demon summons have glowing eyes (except the Felhunter, whose eyes are so tiny as to be practically invisible).
The demonic eredar have glowing yellow eyes (although that differs from their non-demonic draenei counterparts only in colour).
Fel Orcs, who achieved their state by drinking a demon's blood, have glowing red eyes.
In the Tekken series, Kazuya Mishima's left eye glows red as of the 4th game, either because of the Mishima bloodline's Devil Gene, or his resurrection by G Corporation. Mokujin, as well, though his aren't really evil as much as an indicator of evil.
Ridley has a pair of yellow-orange ones; his Space Pirate lackeys share this trait in the original Metroid Prime.
The main villain of Prime, Metroid Prime itself, has glowing eyes, but in deep crimson.
Phazon-corrupted characters in Metroid Prime 3: Corruption sport glowing blue eyes, including Rundas, Ghor, Gandrayda, and Samus herself - the last being visibly reflected in her visor, and growing worse throughout the game.
Dracula of Castlevania fame has glowing red eyes - as if it weren't already obvious that he's not someone you want to mess with...
Joachim of Lament of Innocence and Walter of the same game display this, too, when they're showing the hell off with magic - Walter in a cutscene notably creepier than most of the others.
Although the Hummer robots of Marathon are pretty much a joke, their eyes glow in the dark and may cause pants-crapping. Oh yeah, and what's worse is when you see the juggernaut's eyes... The only difference is that the juggernaut is DAMN FAR FROM A JOKE.
The Heartless of the Kingdom Hearts series, at least those with visible eyes, do this constantly. So does Sora in his Anti Form.
Common in fighting games, especially with possessed or boss characters. Akuma and Evil Ryu from the Street Fighter series always have their eyes glowing red to show they're under the influence of Satsui no Hadou. M. Bison has glowing white eyes in every game he's appeared in outside of Street Fighter II.
Fighters put their eyes all glowy and get a fiery Battle Aura when they grab a Smash Ball, which gives them a somewhat diabolic look. Check out yourself◊.
However, Captain Falcon and Meta Knight already have these to begin with, and Mr. Game & Watch averts this by having no eyes to speak of...
Speaking of Brawl, Link and Toon Link both have "dark" alternate costumes featuring ebony skin, white hair and, yes, glowing red eyes.
In Fable being fully evil causes your eyes to glow red. By this point, you also have demonic horns, insects buzzing around you, a red mist emanating from your footsteps, and townspeople flee at the sight of you.
Subverted with Sparda, who is a demon but had a Heel-Face Turn long before the games to become a sort of savior figure to parts of the human race.
Played straight with Beowulf, whose red eye glows when he Turns Red in the third game.
Also played straight with Dante in the first game and Nero in the fourth.
Some of the characters in The Black Heart have these; Animus' single eye glows yellow, so do Hashi's eyes. Final has two huge glowing yellow eyes, and their glow amplifies whenever he casts powerful magic. In a particularly sinister variation, Janos is always portrayed as a dark silhouette, and generally with a single red eye glowing.
In Doom, the protagonist's eyes on the status bar become glowing and golden when an invulnerability power up is taken (or God Mode is enabled). Combine with a Berserk Pack, and you too can have fun with puns!
Similarly, the protagonist in Wolfenstein 3D — but only on enabling of God Mode (no invincibility powerups in that game).
Several different forms of these are costume options. These range from a face-type (which predates costume auras) with solid-color eyes, to Electric Eyes, Fiery Eyes, Smoky Eyes, Sparkly Eyes, and more.
Also, human-form Kheldians' eyes glow when using their powers as an indication that they're channelling power from their alien halves.
Continuing from the Mobile Suit Gundam example in the anime section, in a number of games, most mobile suits will have their eyes momentarily glow before some of the more dramatic attacks. By the latest SD Gundam game, G Generation Spirits, pretty much every single Mobile Suit in the game will have a freeze frame where nothing moves except the background and the glowing eyes of the attacking unit. Occasionally matching certain suits with certain pilots will give even more dramatic glowing eye shots. The best is whenever Char Aznable pilots his Sazabi, which provides a particularly menacing image. Of course, it IS a Zeon MS....
Sam Fisher of Splinter Cell is nearly always pictured wearing his night-vision goggles, with the glowing green eyepieces as a visual reference to this trope. He's not superpowered, but he's certainly not someone you'd ever want to anger.
In Geneforge, people who use many canisters frequently develop glowing eyes. They're not necessarily evil, but they are powerful and frequently unstable. It may be intentional, rather than a random side effect — a character in the first game says glowing eyes are "designed to engender respect."
The Little Sisters in BioShock have glowing eyes until they're rescued.
The Moon Rabbit Reisen Udongein Inaba from Touhou has these. She has the ability to drive people insane by using her eyes to screw around with wave lengths, which also allows her to make illusions and such. When you fight her, a pair of seriously freaky GIANT RED EYES appear in the background to signal that she's screwing with you. All the more fun, considering that her last spellcard turns her bullets invisible.
The three core characters in Disgaea: Hour of Darkness have these. Laharl and Etna show them when they're angry, whereas Flonne also sports them when she's overly excited. Bonus points for her eyes lighting on fire either way.** Raspberyl also gets these when she's mad.
In Monster Hunter (more specifically Monster Hunter Tri) a hunter can forge the Helios armor set, in which the "eye slots" glow a sort of light blue. It doesn't really inspire doom (in fact you'd probably get some praise from fellow hunters) except for the monster you hunted to make it, an Elder Dragon named Ceadus. Who is nearly 60 meters long and causes earthquakes to an entire island whenever he rams it with his horns.
A lot of monsters also exhibit this themselves, though usually when enraged. All of the aquatic monsters have it when fighting them underwater, which works as a gameplay element to help you see where they are more easily as it is very hard to see underwater, and also to inspire terror as you may not see the monster behind you until it rears its head and shows you the glowing eyes.
A notable example among land monsters is the Nargacuga, who is usually fought at night and whose eyes glow bright red. They glow more intensely when the monster is enraged, leaving trails as it moves around. This is mitigated somewhat if you break its face, which has the result of a scar where one eye used to be, though the other eye still glows.
Lucent Nargacuga takes this a step further: though its eyes still glow, it is capable of turning completely invisible, so its eyes (when in rage mode) are the only means by which you can effectively track its motion.
In Bayonetta, Rodin and Bayonetta are talking in the Gates of Hell Bar, and Rodin is using bottles to describe the relationship between Paradiso, Inferno, and the Human world. When he says that heaven and hell are going "straight for each others' throats," his eyes glow red behind his sunglasses.
Night Trap features these with Sarah (if you're fast enough to see her in the bedroom at the start of the game) and Tony. All of the other vampires have them as well, but only when they show their fangs.
The Doctor Who adventure game The Gunpowder Plot features a shapeshifting Rutan called Lady Winters, who when in human and cat form has bright green glowing eyes.
Standard for anyone on the receiving end of an Ubercharge in Team Fortress 2. The Demoman can get them by taking heads with the Eyelander.
All of the colossi in Shadow of the Colossus have these. When they're simply moving around, their eyes are blue, but when they're attacking or being attacked, they turn orange.
Asura from Asura's Wrath after climbing out of Naraka has eyes permanently in this state, and they glow their former red color on occasion. His master Augus has these naturally, but they are white holes because Augus is blind. They only return to normal once Asura destroys Chakravartin and frees Mithra.
Even Gears of War has this; sometimes played straight, sometimes averted. The Locust Horde (the main antagonists of the series) outfits many of their war beasts with helmets that have multiple glowing eye-pieces to fool human on-lookers that they're fighting multi-eyed, alien beast. The truth is, few Locust creatures actually have more than two eyes, let alone glowing ones. The exceptions being the Kryll, who have two eyes, and the Corpsers, who have four.
A perhaps even greater example of this are the Lambent. Once something is exposed to Imulsion, the series' fictional fuel, it starts mutating into a horrible beast, twisting, shifting or even mummifying... but every single Lambent to date (that has eyes, mind you) has had glowing eyes. Seeing how the Lambent want to consume everything and every time you kill them, they explode, this trope is pretty justified.
In the ending of Alpha Prime, Arnold appears to come back from the dead with glowing green eyes. This is not a good sign for the people he's on the ship with.
Morrighan's eyes in Mabinogi, when they aren't shut, are this trope combined with Death Glare. Needless to say, if you ever see them like this, chances are some serious shit is about to go down.
The vendor in Dust: An Elysian Tail has, as Fidget puts it, "glowing evil eyes of evil". As it turns out, he's not evil at all and quite eager to do business with you.
The last stage of 1985 coin-op Son of Phoenix (aka '99: The Last War) is set in a place which has the Parthenon, the Pyramids and the Easter Island Statues (presumably replicas of these things). At some points of the battle, one or more of the statues' eyes glow red.
Dahlia Hawthorne from Phoenix Wright: Ace Attorney: Trials and Tribulations does this in the first case after she is revealed to be the murderer of Doug Swallow. Also, her mother Morgan Fey tends to do this when she gets angry.
Rider in Fate/stay night when she actually shows her eyes... Probably because they aren't really eyes so much as (apparently) crystallized magical energy that happen to be what she sees with. Oh, and she's Medusa. Figure out the 'of doom' part.
The dragon's eyes and the dragonslayer's eyes in Dra Koi glow blue and red respectively when using their abilities. Or at least, the armor's eyes glow. Comes to the same thing.
The title race of Tarol Hunt's Goblins sport something close when angered. It's not quite glow — it's their widened pupils. Big Ears on the other hand did receive these when he acquired his enchanted paladin gear.
Magic users in Dominic Deegan: Oracle for Hire have their eyes glow when particularly focused.
Tesla: I can't believe you would do something like that! We lost people on the force to glowy-eye-soul-stealing-related complications!
Lackadaisy: While not literally glowing, Viktor's eye is often noticeably shaded lighter than his surroundings would logically cause it to be, giving it a "glowing" look (aka The Glare Beam). This is especially noticeable during the firefight with the pig farmers, which takes place in a darkened garage. During this scene, everything is shaded so as to give an impression of pitch blackness... except for Viktor's scary, glare-y eye. The fact that he's a cat doesn't hurt either.
In The KAMics, the ex-valkyries, Gertrude, Brunhilda & Nikki's, eyes glow when they are angry. Oddly enough they usually glow the same color as their hair color.
The Seven Deadly Sins of Jack have glowing eyes, including the titular character, whose eyes grow black and become pin pricks of light when he's ticked off. The Devil subverts the trope by having completely dark black eyes.
8-Bit Theater: Black Mage. Complete with the hellish aura that was last seen when Black Mage confronted the personification of his sins ( himself), and before that, seen on Black Mage when he took over hell. Not surprisingly, Black Mage was using the power he gained from killing the personification of his sins.
In Wapsi Square, the golem girls and Monica can make their eyes glow, essentially causing them to function as flashlights. It is fueled by fear, doubt, or anger. A golem feeling any of those certainly spells danger.
After Jumping Off the Slippery Slope, Rose's eyes glow white. Inverted with Kanaya, however; after coming back from the dead, everything glows except her eyes.
More literally, Sollux has glowing red/blue eyes — and is a "Mage of Doom"
leveL's main character combines this with some glowing goo oozing from his wrists. Sure, he's always been a pretty timid guy, but when you see a kid that glows that much, and advances towards you with a creepy smile, you have to admit that something ain't right.
In Unsounded, Duane Adelier's[[our-liches-are-different]] eyes occasionally glow.
Aang from Avatar: The Last Airbender has these when he's in the Avatar State. Every young Avatar gets Glowing Eyes of Doom when they enter the Avatar State before they learn to control it. However, when they master it and become full-fledged Avatars, they only need to enter it for about half a second to gather power, then they break out the ass-kicking with normal eyes. This is extremely important, because an Avatar killed while in the State breaks the cycle of reincarnation — there would never be another Avatar again. Avatar Kyoshi is shown using this method (her eyes pulse, then she bends all four elements simultaneously to move tectonic plates) during the episode "Avatar Day". Aang also uses the master method in the finale, after finally learning to control the Avatar State to keep himself from breaking his Moral Code and killing Firelord Ozai.
The Legend of Korra: Korra gets her own glowy eyes in the season finale of her sequel-series, when she too goes into the Avatar State for the first time. It's every bit as awesome as ever.
Spawn in the HBO animated series. That's pretty much ALL you see of his face most of the time. Also Clown/Violator.
The gargoyles develop these when angered; males have glowing white eyes, while females have glowing red eyes. Originally, the writers only gave Demona the glowing red eyes of doom, but the creators thought that would be too silly to base it on evil alignment, and changed it to a natural, sexually dimorphic trait.
The gargoyle clones reverse this: males have glowing red eyes and females white, though this may have more to do with being rapid-aged in tanks rather than their being clones, per se.
When animated Starfire's eyes go from green with green-tinted whites to solid, glowing green, you don't want to be in her way. And once she gets Eye Beams, this happens whenever they're used. (Comicverse Starfire's eyes are always solid green.)
Also, Raven's eyes generally glow solid white when she's using her powers. And if her eyes ever start glowing solid red, it's advisable to run. Especially when they split from two into four. But then, it's probably already too late if that's happened. (Happened in "Aftershock", in season two, without recourse to the Season Four Arc.)
Terra's eyes glow bright yellow when she's pissed or can't control her powers.
This is pretty common in Teen Titans. In addition to the above, Trigon has red eyes that glow constantly, Brother Blood's eyes glow red when he uses any of his powers, Blackfire's eyes are purple and glow when she's angry or charging up a starbolt, and Jinx's glow pinkish-purple.
Similarly, although most of the Transformers in Beast Wars have glowing eyes, Dinobot's normally red eyes shine bright green when he's charging up his Eye Beams. On top of that, they blink by having the lights switch off and then back on, while the actual physical eyes don't close.
X-Men: Animated Cable's left eye glows white when using his telekinesis (or at dramatically appropriate moments) and red when using his cybernetically enhanced vision.
In Danny Phantom the title character can make his eyes glow green when he gets serious or angry; it's one of the effects of his ghost powers. Appropriately mocked by Vlad, the series' resident Magnificent Bastard, when Danny tries to intimidate him.
Vlad: Ooh, the scaaaaary eyes. (fires his eye beams)
The eponymous robot in the DuckTales episode "Armstrong", when it becomes clear that he's turned on Scrooge.
A few episodes end with the band members' eyes glowing red, notably "Performanceklok", which ends with them watching their therapist being ripped apart by wolves.
Mr. Salacia's eyes glow white whenever he uses his Eldritch Abomination powers. Also, Gen. Crozier's glow any time he is being controlled by Mr. Salacia.
"Gosh. Maybe this is weird to say, but... am I the only one who's being made to feel hungry by watching... this? "
Green Lantern John Stewart from Justice League has glowing green eyes as long as he's wearing his power ring — Word of God says that it's because he's been a GL for so long that it's more or less "infected" him. He's also one of the few Green Lanterns who doesn't wear a mask. A few other maskless Lanterns have this same effect.
GIR in Invader Zim. His eyes are always glowing, but when he goes into Duty Mode, there's a brief flash from his eyes.
Gravity Falls: The Gremloblin's eyes glow yellow when people look into them and see their worst nightmare.
In Wakfu, Qilby's eyes gain an eerie glow after he merges with the Eliacube.
The Minions of Set from Samurai Jack have bright blue glowing eyes. They also happen to be super strong, super fast, nigh-invulnerable juggernauts that simply cannot be defeated by normal mortal methods, even with enchanted holy weapons like Jack's sword.
Cats can appear to have this ability, but it's actually more like Shining Eyes of Doom. Cat eyes have reflective membranes (the tapeta) that bounce light which gets past the retina back onto it to increase their night vision. However, the "glow" is all light reflected from the environment and is not a real glow.
Not surprisingly, the pictures on Cats That Look Like Hitler often have "glowing eyes of evil" — in some cases it's probably deliberate.
Parrot eyes don't glow, but they do dilate very noticeably — "flashing". Somewhat akin to a cat's purring or a pet rodent chattering their teeth, this eye flashing might indicate excitement, or warn another that they are very, very PO'd.
Many creatures (most familiarly dogs and cats) have eyes with an organic mirror (the tapetum) behind the retina, to increase the sensitivity by bouncing light back onto it. In diurnal creatures, this tends to be red (not quite the same thing as the red-eye effect in humans), in nocturnal, crepuscular, and cathemeral creatures such as cats or owls, this tends to be orange, yellow or green, and in dogs, this tends to be blue-green or blue.
Humans don't have a tapetum lucidim, the red eye effect is simply caused by the flash being too close to the camera lensnote The light from the flash travels into the person's eye, bounces off the retina, and back into the lens. Placing the flash farther away from the lens widens the angle, making it more difficult for the light to hit the retina and bounce back to the lens. And now you know.. Animals that do have a tapetum very rarely exhibit the red eye effect unless their eyes are blue; instead you may see eyeshine. In fact, humans that DO exhibit something resembling eyeshine (other than the typical red eye effect) in photos should be checked by an ophthalmologist as it is a common symptom of Leukocoria and several other serious (but fairly rare) illnesses.
The Vampire Squid uses bioluminescence to pretend to have these.
There are neon contacts that glow under UV light. Technically, they absorb light at an invisible wavelength and convert it into a visible wavelength before releasing it. This is known as fluorescence.
Modernized T-90 tanks carry a pair of infrared targeting jammers (the Shtora system), mounted on either side of the main cannon like eyes. When idling or out of combat, the jammers are off and appear to be regular old searchlights. In combat, they are switched on and emit a sinister red glow◊.