Markiplier (repeatedly dying at the hands of a ridiculous-looking monster):MOTHER... AAAAARRRGH!!! I'm so mad. I'm soOOO MAAAAAD!!! UUUUUEEEAAAAAAAGH!!! NO! FUUUUCKING HELL! I've got a... I'VE GOT A... LET ME OFF!!! DAMMIT!!! DAMMIT, DAMMIT, DAMN YOU TO HELL! YOU SON OF A BITCH!!! YOU'RE DEEEAD! Everything! I hate everything! I HATE EVERYTHING!!! GEDOVAHRDEHRYOUFUCKINGFUCKAHR!!! I'm gonna, I swear... I SWEAR! LET ME THROUGH!!!
A running gag on his Dead Space 3 walkthrough is that he constantly mentions or talks about Ellie's boobs.
YouTube Commenter: After 5 minutes it sounds like some kind of a dark acolyte summoning a demon..
In another video Mark attempts to read a note in the foreign language he quips he accidentally summoned a demon into the viewer's home. In later episodes of the same series he continues to apologizes for it and gives helpful suggestions on how to deal with their new resident.
In his playthrough of the Amnesia: The Dark Descent full-conversion mod Killings In Altstadt, there is a soundtrack bug that causes the background music to be stuck in a loop of a cheerfultropical tune, which is the Shop theme from Ocarina of Time, and Markiplier is forced to play with the bugged music for at least another ten minutes. This eventually drives him insane enough that he tries to kill the main character by jumping off a tall balcony. Repeatedly. While crying.
Becomes especially funny near the end, because the first time he jumps off the balcony, it does stop.And then it starts all over again. It's like the game was being a troll to Mark.
During the last segment of Amnesia: Rain, Markiplier thoroughly explains how much he loves meat. And then this slips out:
Markiplier: "My mouth is just watering at the thought of that giant juicy sausage! Ahhh, I can't wait for the flavor to blow in my mouth..."
In Slender: The Arrival, Part 3 when he first encounters the Slender-proxy he repeatedly states that he has a flashlight ("with your name on it!") The actual encounter is very brief, with Markipilier screaming the following: "You're not supposed to do that! You're supposed to be blinded - be blinded, you DICK! THIS IS NOT WORKING!"
When it actually does work on the second try, the normally PG Markiplier breaks out the big guns: "Get blinded, you bitch! Be blinded! Yeah, that's right, suck ten thousand cocks right there, in your EYEBALLS!"
During yet another attempt at the alien surgery, he manages to finally disconnect one of the body parts, and, to his horror, hears it making noises. His commentary beforehand grinds completely to a halt.
At one point Mark loses all hope of winning the game when it gives him the message that 'the fish have been over-fished' and he can only fatten up on berries. He then drives over a pond...and a ridiculously large fish falls into the car. It's so big, it fills up the entire dash and Mark can't even eat it because it gets wedged outside of his reach by some rocks that had fallen into the car earlier.
His Drunk Minecraft series can be full of these. Like this example from the first episode when he his considering getting rid of the webcam, to the others' protests.
Lord Minion 777: Everyone wants to see how stupid you look while you play. *beat* That sounded meaner than it did in my head.
Mark: I will shove a rake up your anus.
Toward the end of the series, Mark, Wade and Bob start getting royally smashed, which culminates in hilarious moments like Mark and Wade goofing around and losing their shit at every opportunity for no reason whatsoever:
Wade: Hey, uh... what's this episode about before we start?
The third part has Mark being so startled by a Jump Scare (namely, the toy soldier shooting Viola with a real gun) that he falls out of his chair and takes his webcam down with him.
All 30 minutes of the "Where Is Wade!?" episode of Drunk Minecraft. A quick recap: Mark and Bob slaughter a village of innocent moon aliens; discover that Wade has been turned into a block of sand; create a railroad to transport Wade's body back to their spaceship; Markiplier goes insane due to having Wade's body in his possession; and they finally make it back to Earth and create a monument to Wade.
The last five minutes of his playthrough of Mental.
His initial excitement when thinking The Curse of Blackwater is a pirate themed horror game, and his angry disappointment when he is told it's not.
Any time where he ends up playing a game that either features a Shout-Out or is directly based off of him in general. One good example is when he plays a mod of SCP - Containment Breach... based off of him. He spends a few seconds at the start of the video in stunned silence, and later comments that it brings a new level of disturbing to the game.
When he finally beats the game, his maniacal laughter breaks down into a confused sob.
And then there's his brief Octodad-related freakout at the end of Catlateral Damage:
(smooth voice) And now we know there's more to life (starting to rant) than screaming at a stupid freaking octopus that doesn't know what he's doing and won't ever cooperate with you, (starting to shout) and will never do what you say-
Every time he plays a game where you have to destroy yourself, such as Skate 3 or Turbo Dismount. Crowner goes to this accident, where he manages to get sandwiched between two camions. And he doesn't realize it until he sees the replay.
Tensions ride high however when Crusader JonTron agrees with Wizard Markiplier that Witch Doctor Cry shouldn't call himself a Witch Doctor if he can't even bring Uber back from the dead! I mean seriously, what kind of Witch Doctor has zombie dogs but can't make a zombie Monk? That is ridiculous!
His commentary after a staircase collapses from underneath Mandus:
MARKIPLIER: I knew it was gonna be a trap! I had a feeling... then I had that other feeling that you get when you land on your ass, and your legs go inside of you! It's a bad feeling...
When Markiplier meets Yamimash at Pax on his channel it starts with them dueling in an inflatable american gladiator style battle with Yamimash repeatedly losing Mark rejects his offer of a hug at the beginning pretending to be a heel he then later offers Yami a hand up and and pushes him over his battle stand. However on Yamimash's channel we're treated to Yamimash spotting Mark and eagerly calling out for him. Mark gets lost in the crowd and Yamimash sounds dissappointed until Mark reappears and after a brief discussion Mark pretends to get overly emotional. Then it cuts to the words "Edited Version" The video is shown in a purple tint and saxophones play "I will always love you"
There's a "neckbeard" themed version of Flappy Bird, called Flappy Fedora. In Mark's playthrough of it he spends the entire video doing an impression of MRA/pick up artist types, only to repeatedly get interrupted by the game.
In his playthrough of OFF, Mark scolds the foul-mouthed Dedan after the latter calls the ghosts invading his office "phantoms of shit". Four minutes later, he gets stuck on a puzzle.
"Monacle monacle, tophat, monacle!" Actually, all of Mark's attempts at a British accent. They're massively overdone. Jordan isn't much better admittedly, and slips out more frequently. Mostly because of laughter.
Jordan shooting himself in the foot, complete with laughing fit.
The Call Back to his attempted playthrough of Cold Fusion in his Let's Play of Outlast Whistleblower:
Mark: Those brass, they really like to pump up the atmosphere. This game doesn't rely on a string quartet on my back [as Cold Fusion did every time there was a Jump Scare], just has a guy playing a sousaphone in my ear- (said sousaphone makes its presence known) Stop that, Sousaphone Man!
His moments of Wrong Genre Savvy throughout Among the Sleep, particularly his assumption that the teddy bear is the one responsible for all the horrible things when it's actually the mother. This results in close to every statement the teddy makes making Mark uncomfortable and suspicious.
At one point in his playthrough of It Moves, he makes his character interact with what appears to be a red-faced young girl dressed in a green cloak that audibly growls and stamps a demonic face on his screen, which leaves a faint after-image. It takes Mark two minutes to notice it.
The entirety of his FNaF playthrough is hilarious (well, when it isn't being terrifying), he hurts himself multiple times during the jumpscares, his reaction to Golden Freddy appearing in the security room, at one part he speaks about some of the fanart being produced of the animatronics, and when he unlocks the Custom Night he immediately sets all of the robot's AIs to 20 and gets killed three times in the first hour.
At one point on the 2nd night, he gets very worried and says this:
Mark: Ooh I'm not gonna have enough power to survive the night. My butt is gonna be munched, I'm gonna be shoved into a teddy bear outfit, and they're gonna laugh!
After spending seven hours trying to complete 4/20 mode on the 7th night (all 4 AIs set to a difficulty of 20), he finally completes it and explodes with joy.
On the fifth day, he expresses confusion when the phone starts ringing, since the Phone Guy died yesterday. When the call picks up, he drops his jaw in horror as it plays horrific noises. And then Foxy decides to pay him a visit when he least expects it.
On one of his 5th Night attempts, Mark yet again ran out of power, and to avoid hearing the loud, mechanical squealing sound of Freddy's attack, he took off his headphones and held them far away from him while looking at the camera. After a brief time cut, Mark starts looking confused as to why he hasn't been killed yet and tentatively starts bringing the headphones closer and closer while looking at the game once more. Guess what happens the instant the headphones are about an inch from his ears?
And then Mark and friends play a horror map in GMOD as the cast of Five Nights at Freddy's. Memorable moments include Mark-as-Freddy making an impression of his trademark Evil Laugh, and him constantly blowing up Wade (as Foxy) each time he grabs a puzzle piece and taking it from him to add to the wall.
"I really don't wanna cross the threshold of slam... [Wade runs towards Mark] But Wade is! [Wade tries to cross, blowing himself up]"
Everything after they give up trying to solve the puzzles and cheat, especially when the Gravity Gun comes into play.
Mark's video of Mole Hammers with Wade, especially his meltdown at the end after losing badly to Wade.
And then there's the sequel, in which Mark redoes the intro every time he loses.
Mark plays again Table Tug with Wade. Starting from this moment, he begins recounting the history of the Tit Tanic. It has to be heard.
Another incredible moment happens when he throws a noisemaker... and it clips the desk in front of him and falls right next to him!
He threw another noisemaker, really far away... and it clipped itself onto a reactor and back to him. He got up, got out, and gets bum-rushed by the Alien, who pushes him to the floor.
The third time's a chance - he sent another noisemaker at a lower level, which attracted the Alien. Mark, being Mark, embellished his hope that the Alien would focus on the noisemaker more than him, so he said "Stab it! Stab it with you Tenus Pale!" He meant Penis Tail. The look of instant confusion on Mark's face is priceless.
The end of GMOD Murder #14. Bob is on a ledge with a gun pointed at Wade, Mark, and Jack, and says that if the murderer pulls out his knife, he will shoot one of the bystanders and leave the remaining two to duke it out. Mark, who is currently the murderer, pulls out the knife, and Bob immediately shoots him.