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Some people take this trope too literally...
NOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!
Almost Everyone On This Page

In real battles, I think people probably respond to trauma either by going catatonic or with black humour. But in movie-land, when a friend is wounded, the Deep Magic says you have to yell his name. (If his name is "No!", that means he's actually dead.)
Andrew Rilstone

"... the short answer is no. The long answer is... NOOOOOOOOOOOO!!!!"
Strong Bad, independent

The moment when a character realizes that something awful has happened, or notices that something awful is about to happen, and screams "NOOOOOOOOOOOOOOO!" If the character is rushing to prevent the something-awful from happening, a slow-motion effect can be employed to draw out the tension of the moment, sometimes to the point of drawing out the "No" into a comical bass growl. (Editors with more foresight will combine this trope with the one that indicates a character in shock by muting the soundtrack.) This trope is very susceptible to becoming Narm, but does raise the question of exactly how a character should handle a crisis without coming off like a weakling (by going catatonic), a monster (by cracking a grim joke), or too crude to allow on air (by swearing one's head off).

Used both seriously and for comic effect. Very common on sitcoms. Sometimes the Big No ends the episode with a Cliff Hanger.

Nearing the point of becoming a Discredited Trope. A little more commonly played straight in Japanese media, due to the equivalent iya's higher screamability. Not very common in Russian, due to the equivalent nets very low screamability.

Occasionally the character will shout something other than "No!" — usually shouting the name of the character that something bad is about to happen or has just happened to. This is slightly less discredited. A shorter but still intense "No" also seems less absurd than a drawn-out howl. If nothing less than a howl, expect a Skyward Scream.

When a villain screams the Big No, it's usually as his plans are crashing down around his ears and is usually the last thing he ever says.

Large Hams will pull one out a Giant No if given the opportunity.

A Sub Trope of Big Word Shout.

Compare/Contrast Little No and Big Yes.

See here for a side-by-side comparison of a LOT of Nos.

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