Voiced by: Wally Maher (classic shorts), Charlie Adler (Droopy Master Detective), Paul Reubens (Tom and Jerry's Giant Adventure)
- Adaptational Name Change: He's called Screwball Squirrel in Droopy, Master Detective.
- Killed Off for Real: The ending of "Lonesome Lenny".
- Screwy Squirrel: The Trope Namer.
Voiced by: Bill Thompson (Blitz Wolf), Frank Graham (40s shorts), Daws Butler (50s shorts), Frank Welker (Tom and Jerry Comedy Show, Tom and Jerry Kids, Droopy Master Detective), John DiMaggio (Tom and Jerry: Robin Hood and His Merry Mouse)
- Anti-Villain: When he simply represents lustful men. When against Droopy or the three pigs, he's quite villanous.
- Big Bad: To Droopy in both The Tom and Jerry Comedy Show and Tom & Jerry Kids.
- Eye Pop
- Villain Protagonist
- Wild Take
- Wolf Whistle: The Trope Codifier.
Voiced by: Sara Berner (Red Hot Riding Hood, Swing Shift Cinderella), Bea Benaderet (Shooting of Dan McGoo, Wild and Woolfy), Imogene Lynn (singing voice), Teresa Ganzel (Tom and Jerry Kids, Droopy Master Detective), Grey DeLisle (Tom and Jerry movies)
- Adaptational Name Change: She's called Miss Vavoom in Tom & Jerry Kids, where her hair color also changes from episode to episode.
- The Cameo: In Quest for Camelot.
- The Chanteuse: Plays this type of role in all of her original shorts.
- Gainaxing: Prevalent on Red's ample chest.
- Glamorous Wartime Singer
- Hello, Nurse!
- Hotter and Sexier: Very much so when compared to the usual little girl type character that plays Little Red Riding Hood.
- Interspecies Romance: Sometimes with Droopy Dog.
- Lady in Red
- Ms. Fanservice: The trope codifier.
- Sexy Backless Outfit
- She's Got Legs: And how!
- Shout-Out: In her original short, speaks in a Katharine Hepburn type voice.
- The Tease
Voiced by: Billy Bletcher (40s shorts), Bill Thompson (50s shorts), Jim Cummings (Tom and Jerry: The Magic Ring), Jeff Bergman (Tom and Jerry Meet Sherlock Holmes), Joe Alaskey (Tom and Jerry: Wizard of Oz movies)
- Bully Bulldog
- Cheaters Never Prosper
- Oireland: When he speaks, it's usually with an Irish brogue.
- The Rival: To Droopy in many of his shorts
George and Junior
Voiced by: Dick Nelson and Tex Avery (classic shorts), John Rubinov and Tony Pope (What a Cartoon)
- Ass in a Lion Skin: George as a worm and Junior as a chick in "Henpecked Hoboes"; George as a dog in "Hound Hunters".
- Big Guy, Little Guy
- George: "Bend over, Junior."
- Junior: "I done a bad thing."; "I'm bend over, George."
- The Ditz: Very much so with Junior.
- Literal Ass-Kicking: George's preferred method of punishing Junior.
- Shout-Out: Based off of George and Lennie from Of Mice & Men.
- Those Two Guys
Blackie the Cat
Voiced by: ???
- Ass in a Lion Skin: Disguises himself as a dog in "Counterfeit Cat".
- The Jinx: In "Bad Luck Blackie".
Voiced by: Daws Butler
- Captain Obvious: Has a habit of pointing out what is obvious to the audience.[After failing to demolish Droopy's doghouse] Now that's a well-built doghouse, man.
- Southern Gentleman
- The Stoic: Barely reacts to all the injuries he suffers, even a guided missile to the butt.
- Verbal Tic:
- Has a habit of ending sentences with "man".
- In "Billy Boy" his lines repeat at the end Like A Broken Record.