This has a tendency to happen in an Improbably Female Cast where varying male personalities are limited and in Yuri to give the Schoolgirl Lesbians and the audience one more androgynous girl to drool over. This usually takes the form of one girl being a Lad-ette personality-wise and the other girl will be a more refined Prince Charming complete with a Bishie Sparkle. Other variations exist as well, such as the likelihood that one may be more athletic while the other one is a Bad Ass Bookworm.
As with any two character trope, the two characters have to have some sort of relationship with each other, be it friends, family, love interests, or rivals for a love interest, so the two characters can be playing the traits directly off each other. Expect a Romantic Two-Girl Friendship if it's a Yuri. Expect an Odd Friendship regardless. Sometimes the girls will not get along and there will be moments of rivalry or even Belligerent Sexual Tension between the two.
Contrast the polar opposites, Light Feminine and Dark Feminine — two girls who are both feminine, but in different ways — and Noble Male, Roguish Male — two guys who are both masculine, but in different ways.
- In Ai Ore! Love Me! Considering almost all of the members of the band Blaue Rosen are girls that fit either the Bifauxnen or Ladette aesthetic or both, this trope is a given.
- Sylia Stingray and Priss Asagiri in the Bubblegum Crisis OVA. They're more of a Tomboy and Girly Girl deal in Tokyo 2040, however.
- In Hellsing, Integra Hellsing, Rip Van Winkle and Zorin Blitz are all masculine women with distinct and opposing personalities.
- In Monthly Girls' Nozaki-kun, we have the Yu Kashima as the Prince Charming Bifauxnen and her "singing coach" Yuzuki Seo as the foul-mouthed and abrasive ladette. As Seo does not actually look masculine, they were once mistaken for a couple when seen hanging out together by Wakamatsu who assumed that Kashima was a boy.
- Pictured above: Kaoru Orihara (brunette, on the right) and Rei Asaka (blonde, on the left) in Dear Brother, arguably the archetypal examples. Rei is a stoic depressed loner that sometimes gets mistaken for a man and talented basketball player and artist; Kaoru is an extremely androgynous Lad-ette that is just as good in basketball as Rei is, not to mention she's arguably the only sane person in the story. While they are both athletes, Kaoru takes basketball much more seriously than Rei, who is too doped up to care much about anything, though when she does play, it seems to be the only healthy source of enjoyment she has. Coincidentally, that happens when Kaoru is having a relapse in her illness and Rei takes her place to help the team. Also alluded to in their "titles". Rei is called "Hana no St. Juste" which means "St. Juste of the Flowers", while Kaoru is called "Kaoru no Kimi" aka "Prince Kaoru".
- PriPara: Hibiki plays the Bifauxnen of this dynamic with both Shion and Dorothy.
- In Sailor Moon, Haruka Tenoh and her rival Seiya Kou plays the trope straight in the manga but only plays with it in the first anime considering that Seiya is a Gender Bender in it. (So she only played it straight in her Sailor Starfighter identity, which is the original one.)
- Wonder Woman (1987): Cassie is a teen with Boyish Short Hair and a spunky attitude who wears loose baggy clothes and is easily mistaken for a boy, while fitting the visuals of an attractive teen boy for her time period, while her best friend George is more intentionally androgynous and has a more delicate and traditionally attractive appearance.
- X-Wing Rogue Squadron: Plourr Ilo is the gleeful, bloodthirsty Boisterous Bruiser who loves to tease her friends and tends to take charge; she's also secretly a princess. Xarcce Huwla is soft-spoken, cultured, possesses an understated sense of humor, and is restrained. They're both big and muscular and proud of their fighting skills, and Xarcce's species has little sexual dimorphism, and they both play The Big Guy at need. The two are shown having a friendly sparring match, with padding, and then go drinking together.
- Chronicles of the Kencyrath: Jame (the protagonist) and Brier Iron-thorn (her Number Two). Both are androgynous badasses, but they're still quite different from each other. Brier is the Stoic, very military and highly disciplined, and Jame is kind of a loose cannon, and a Walking Disaster Area Anti-Hero. They have a Red Oni, Blue Oni thing going on, and stern Brier is the Conscience to Jame. Physically they contrast too, though both are bifauxnens. Brier is tall and sturdy, with Boyish Short Hair, and Jame very skinny and waif-like, with Rapunzel Hair.
- A western lesbian example can be in The L Word:
- Shane and Carmen: Shane is a famously androgynus-looking alpha-lesbian. As a young abandoned orphan, she used to make a living as a prostitute, working with gay, male clients. Personality wise, she can be seen as a gay female take on The Casanova. She is very charming and suave. Her girlfirend, and later fiance Carmen, is a tomboyish Hard-Drinking Party Girl of the Spicy Latina variation, who works as a DJ. She is blunt and very energetic. They make somewhat of a Red Oni, Blue Oni pairing.
- The show also features a non-romantic example: Tasha — The Squadette, who gets very Biaxfunen in her National Guard uniform — and her BFF, Papi — The Lad-ette. Personality wise, this pairing plays this trope pretty straight. Tasha is very quiet and has surprisingly traditional values — which is often lampshaded. She enjoys treating her love interests like an old-fashioned male suitor would. Papi, another Spicy Latina, is a total player, and a very clear example of The Lad-ette. She playes high stakes poker and basketball, drinks a lot and even outshines Shane in the field of random hookups.
- Star Trek: Deep Space Nine: Jadzia Dax is a cool, suave, worldly, Ace Pilot with several lifetimes of experience behind her. Kira is a grizzled ex-resistance fighter wrestling with her home planet's tragic past and uncertain future. There's no tension between them, sexual or otherwise, just friendship and advice.
- Paragon Femshep from Mass Effect has this dynamic with Jack. Paragon Shepard is composed and diplomatic. Jack is violent and snide.
- Another possible Bioware example is Sera and any androgynous-appearing female Inquisitors from Dragon Age: Inquisition; Sera is crude and boyish, telling her friends rude stories and revelling in playing pranks, while the Inquisitor can be much more reserved and severe. Even if they're dating each other (which may prompt the Inquisitor to get involved in Sera's antics), the Inquisitor's dialogue generally has her be a lot more level-headed and polite.
- In Pokémon GO, Blanche of Team Mystic is a woman who dresses well in an androgynous fashion (along with her ponytail), and acts in a more analytical tone. Candela of Team Valor has shorter hair and lean body with ample bust, and relies on strength, but is easily enamored by the small, cute Pokémon.
- Steven Universe has Garnet and Amethyst, of the core Crystal Gems. They both have relatively masculine personalities compared to Pearl, but Garnet is a mature and composed Team Dad-style team leader, whereas Amethyst is a crude and playful tomboyish "rebellious teenager" type (despite being thousands of years old).