Under certain conditions, it's possible to have Cuccos fight for you during adventure mode. Cue an entire army of chickens fighting against your enemies.
It's possible to encounter two Cuccos fighting each other over a keep. If the fight's not been intervened with in time, one of them will win, populate the keep with an army of Cuccos, and then act as a third faction, complete with them trying to conquer other keeps. (If you do intervene, you get locked in the keep with a horde of angry Cuccos.)
Golden Cuccos may sometimes spawn looking for food within keeps. If left on their own for a while, they'll eventually turn giant and attack.
At the end of one trailer, Lana apparently incurs the wrath of the dreaded Cucco's Revenge Squad. This turns out to be one of her weapons called the Summoning Gate, the August Nintendo Direct showed her using it to summon a humongous Cucco out of it to attack enemies as a special move. In addition, they affect gameplay. One mistake, and the entire screen is filled with murderous fowl. Doing something right, however, allies an army of chickens with you.
The Boss Pack has an unlisted unlockable: Cucco's Fury. You play as a giant Cucco, and it's as hilarious as it sounds.
Linkle's army in story mode is made up of Cucco ground troops. And, without any hint of irony, she uses the phrase "poor, defenseless Cuccos."
It's particularly funny when the camera doesn't accompany him due to cramped spaces, and you see Link doing... nothing for the special attack, and still dishing out a lot of damage.
Also worth mentioning is when Link pulls the giant pillar from Ocarina of Time out of the ground and starts using it as an actual weapon. By suplexing it.
The victory pose for the Ball and Chain/Gauntlets is Link causually tossing the ball in one hand. He tosses it to the ground, and it lands with enough force to pop him into the air. Link looks at a gauntlet in amazement, as if to say "Geez, how powerful are these things?"
Using the Hookshot results in your warrior getting dragged towards the target. Using the Hookshot as Link using the Horse weapon results in Epona getting dragged along with him.
The Moon attack is darkly funny. There once was a time where players did all they could to keep it from falling out of the sky. Now they're the ones making it crash, just to kill enemies.
Ghirahim's playable demo at the Nintendo direct showed him doing his infamous "Happy Dance", except unlike Skyward Sword where he did it because he was about to revive Demise, Ghirahim seems to be doing it for absolutely no reason. He's dancing about in preparation to use the Gate of Souls to call in the Imprisoned... and cuts it short when he realizes his Turncoat Soldiers have been found out.
One of Zant's attacks involves him growing giant and doing a belly flop.
The official guide even describes it as being more like throwing a tantrum than actually fighting.
The fact that he will yell "Ow! Ouchies!" sometimes when he gets hurt...
The Majora's Mask DLC gives him the Troupe Leader Mask to wear... which wouldn't be so bad if it wasn't for the fact that he's wearing it over his helmet.
Ganondorf's disgust at Zant's childish begging for help when in danger.
Ganondorf: Help? Aren't you a king in your own right? And you need help from me?
One the new features from the upcoming Ver.1.2.0 update of the game is a new sword and shield set for Link. Said set is the original wooden sword and shield from the very first Legend of Zelda game, accurately depicted as they originally were... as literal 8-bit items. The Master Quest DLC has 8-bit weapon unlockables for other characters now as well. Impa's is labeled "8-Bit Boomerang?", and yes, the question mark is indeed part of the actual name. Sheik, on the other hand, can get an 8-bit Ladder that is somehow utilized as a Harp, while Ganondorf gets 8-bit keyblades.
In the North American version, the fairy who warns Impa and Sheik is identified as Navi, complete with "Hey! Listen!"
In Darunia's victory video, he tosses his hammer into the air before a remix of Saria's Song plays, making the Goron bust out the iconic Happy Dance. After dancing for a minute, the hammer finally lands, causing a shockwave that tosses the still dancing Darunia into the air.
The scene with Great Fairy in Skyloft. She has a bunch of fairies hold a fishing rod with a giant pumpkin on the end, and Levia comes up and bites it like a fish. Then he proceeds to rain lightning onto the battlefield, weakening Volga in the process. Then he complains that he's given you enough divine intervention.
When Link meets Fi in Skyloft, he looks... generally confused.
Fi: You are not the master, but I can consider you a master.
(Link looks at Fi with a "what the hell are you talking about?" expression)
When Link, Lana, and Zelda are saying goodbye to the rest of the group, Midna chimes in.
Midna: Well, that's that! See you next crisis!
Note that she does indeed wind up being called back along with the other heroes from other Eras to help take down Ganondorf. Her entrance is funny, too, as she floats in while in pretty much a relaxed position, summing up her character perfectly.
Some of the things your allies say can be very amusing depending on who you're playing as:
If you are playing as Ghirahim during certain levels of Adventure Mode, the other Ghirahim that is supposed to be helping you will quickly betray you. They may then proceed to have a verbal jousting match with each other.
Proxi telling Link not to blush after receiving a compliment from Ghirahim or Ganondorf?
Fi tells Ghirahim that he's a true hero of Hyrule during one Adventure Mode situation, to which he responds, "Of course! Would you expect any less of me?" ...um, yes?
Volga's reaction to having to save Skyloft during Cia's conquest in the Master Quest DLC; he's very obviously not happy about having to help a bunch of humans. ("I'm here to... ugh... rescue you.") The funny part comes in the Skyloft Captain's extremely cheerful response to this offer.
Twili Midna and Imp Midna in a battle at the same time is just about as funny as with Sheik and Zelda if they compliment each other or give each other some back-and-forth banter. It's even better if they're on opposite sides.
Midna: You think you can beat me? Heh, you can't be serious.
Darunia's line during the Water Temple: "Let's pour into the Water Temple! Get it? Water puns!"
A little bit of Fridge Humour: the locations of the pieces of Ganondorf's splintered soul. In the Skyward Sword and Twilight Princess eras, the location is the Disc-One Final Dungeon of those eras* the Sacred Grounds and the Twilight Palace, respectively and the location in the Ocarina of Time era is the Water Temple.
The glitch's outcome turns out to be intentional. A number of the Twilight Princess DLC Adventure Mode missions involve fighting opponents who use a different character's moveset, most commonly Cia using Lana's moves.
Lana's Summoning Gate is funny even when not involved in the weapon-swapping glitch. Almost every summoned boss causes Lana to fall over when called upon, and when working with Manhandla, Lana will launch into a little song and dance the whole time!
Fi is pretty confident when entering combat with the player, declaring that the player's chances of defeating her are 0%... Even when she's one inch away from death and you are at full health.
Cia's reaction to Wizzro's first betrayal is to snarl, "Am I really being betrayed by evil jewelry?!"
Earlier in that same stage, there's Volga's response to Cia's initial attempt to recruit him:
Link's costume for the Twilight Princess DLC pack is... The Postman. Complete with booty shorts and a befuddled expression on his render clearly saying "Who asked for this and why."
One of the rewards that can be gotten by scanning an amiibo is 1 rupee, as in, a single rupee in a game where you'll get several thousand in every level without even trying. It's lucky that each amiibo yields a new reward everyday.
Ruto has a specific portrait◊ that only appears during certain situations involving Link (for instance, if he rescues her when she's at low health), which has her folding her arms while blushing. It's pretty clear she still carries a torch for the Hero of Time, and this Link reminds her all too much of her own. Midna has a similar portrait,◊ but it appears to have been Dummied Out.
Young Link's C5 has him dash about with a pair of Pegasus Boots in the shape of the Triforce. This attack... doesn't change at all when he puts his Fierce Deity's Mask on. An adult-size Hylian dashing about like a kid on a sugar high looks hilarious.
Tingle's attack animations have the Japanese weirdness he's known for along with Tingle himself getting hurt in humorous ways. One attack involves his Balloon springing a leak and flying out of control. Hitting the Heavy attack again causes it to slam Tingle into the ground, taking out all enemies around as the 35-year old "Fairy" twitches in a Tingle-shaped hole.
The new pack includes extra costumes of the characters bearing masks. Cool and all, like Impa with the Mask of Truth, Link with the Fierce Deity's Mask, etc. What is Midna's? A Continuity Nod to Twilight Princess' Ordon Shield when she wore it like a mask! And Fi's? The Stone Mask!
In the 3DS version, one of the King Of Red Lions' attacks is to transform into his boat form and ride over enemies, while hopping around similarly to how the boat could jump in Wind Waker. It's as goofy as one would expect.
Linkle's No Sense of Direction. For the majority of her campaign, she tries to go to Hyrule Castle, only for her to aimlessly wander into other areas despite having a map. It takes a total of five tries before she finally arrives at the castle.
Her victory animation with the Crossbows even makes fun of this, with Linkle pulling out a map and going in the opposite direction of Hyrule Castle as indicated by a sign. She does this after every successful battle, even after battles on Hyrule Field with the castle in plain sight.
Linkle's second level takes place in the Water Temple, and it starts with Darunia closing the front gate. A tactical decision to keep the enemies from coming in? Nope, he accidentally broke the door.
In Linkle's third stage, when she confronts Ghirahim after stumbling upon and subsequently foiling his plot to release the Imprisoned, he angrily demands to know who she is... and what is she wearing? In addition, Linkle's rebuttal is a Call-Back to one of Ghirahim's lines from Skyward Sword, referring to how the sight of Groose's appalling hair makes his gorge rise.
Medli's Special Attack does as much damage as it needs to...before Medli falls on her face and does her classic dizzy animation from her home game. Same for Toon Link, who gets winded after using his weak point smash attack, and dizzy when using his Special Attack.
The Link's Awakening Pack has Grandpa Ulrira as an Item Card. You heard that right: you are using a shy old man as an item for progression.
Linkle's victory animation with the Boots has her performing badass kicks and moves, before a Cucco sneaks up under her foot and causes her to lose balance. After she falls, the Cucco wastes no time in jumping and setting on her head. It's as funny and adorable as it sounds.
Skull Kid's costume from the Link's Awakening pack is a hard reference to place, but his green hat and bright yellow clothes lead some to theorise he resembles a banana (which was indeed an item featured in Link's Awakening).
The Reward Map for the Koholint Island DLC has a couple of moments: The image is Marin singing blissfully unaware while Link and Lana are rushing to intercept Wizzro and a series of Giant Bosses. In the level itself, Marin is the Allied Commander, and has this reaction when the enemy begins to directly attack the Allied Base.
Marin: The monsters are heading this way? I'll take them on! I mean... Eeek, help!
Ravio's victory animation has him swing his hammer twice, barely able to stay upright. After the second swing... he kinda just decides that it's fine where it is and lets go of the handle, then sits down and reclines the way he does after he's sold all his items.
In one mission in Legends Mode, you're facing Zant when Argorok comes out and starts shooting fireballs at your base. After two attempts to chain it down failed, you try a third time using the fairy fountain. At first, it just breaks free again, until a final chain comes out and pulls the freaking moon from Majora's Mask down. It's later revealed to have been made of fairies and seemed to be a nod at how sick everyone was that it kept getting away that magic itself got fed up! Considering how annoying Argorok was up to that point, you can consider this a hilarious Take That, Scrappy!.
One of the possible quotes Aryll has when the player encounters Toon Link is a confident boast about how her big brother can easily defeat you. The funny part? The portrait it uses has Toon Link absolutely dreading what's to come, suggesting that she's writing checks his courage can't cash.