Women who operate fast vehicles are apparently attractive. Maybe it's the tightleather. Also includes car drivers and female pilots.
Compare and contrast Hood-Ornament Hottie. The two are similar, but a Biker Babe actually rides the bike and often wear outfits which are a little more conducive to road safety. (Although the page image is an exception to this rule of thumb.)
May deliver a "Samus Is a Girl" reveal, if she doesn't wear her leathers skintight and does wear a full helmet.
Essentially the Distaff Counterpart to Badass Biker, in that she won't be a pushover if things get messy, although she may be less aggressively badass than he is.
Will likely induce a Hello, Nurse! effect on nearby males, and maybe some Stupid Sexy Flanders from a nearby female.
Compare Hood Ornament Hottie who is purely decorative.
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Anime and Manga
Appleseed does this with the main character, Deunan. She is seen in the Ex Machina movie riding a pink motorbike around the city of Olympus. This depiction isn't shown again in the movie, but gives an insight into her personality.
In The Castle of Cagliostro, when the climax is over, Fujiko drives away in her motorcycle with the Goat Bills plates in her bags.
Masayo, a one-time character in Tenchi in Tokyo who befriends Ayeka and Sasami. She was a shooting star turned into a woman by Yugi and their squad, and had to befriend the girls to keep them from findign Tenchi. But after they learn that she's also searching for her also-humanized boyfriend, Isao, the sisters help her find him.
Celty from Durarara!! uses a kind of glamour to shift her horse and traditional attire into this as a modern-day version of the Headless Horseman.
She never does it in the actual visual novel, but Arturia, the Saber from Fate/stay night, is one badass biker babe. Turns out her ability of being a supernaturally adept "rider" isn't limited to animals...
Azami of Hatoful Boyfriend rides a scooter, but she used to be part of a gang called Hell's Birdies. What she loses in coolness for being a very meticulous, careful driver she regains in how she fights.
Roxy Rocket of the DCAU tries to be this. Whether or not she succeeds is an issue to take to the forums.
The aptly named Sizzle (both for her measurements and for her love of flamethrowers) from Stunt Dawgs was built around the concept of hot women on hot bikes.
AndrAIa from ReBootreboots as a literal biker babe on numerous occasions, complete with leather fixings, and retains a fascination with motorcycles and hover-bikes throughout the rest of the show. This part of her nature has not gone unnoticed, and many other characters frequently ask just what it is with her and bikes.
Arcee of Transformers Prime uses a hologram of a helmeted Biker Babe in a skintight leather outfit as part of her motorcycle disguise when she's in public. This causes problems for Jack in one scene when his crush assumes that Arcee's "rider" is his girlfriend.
The villainess Rojo in the original Ben 10 series.
Jill Rivers (Tara Buckman) and Marcie Thatcher (Adrienne Barbeau), two knockout women in a black Lamborghini Countach in The Cannonball Run.
At least start with Joann Nail and Susan Flannery from the original (and superior) The Gumball Rally.
Thunderball had Fiona Volpe and her motorcycle; the remake Never Say Never Again had Fatima Blush, who led Bond on a chase in a (heavily modified) screaming mid-engined Renault 5. Volpe herself got Bond a bit anxious as a passenger in her Mustang.
In The Fast and the Furious series, virtually all of the female characters are this, even Mia who is otherwise much less badass than most of the guys.
In Corvette, the player periodically gets into race Challenges against an assortment of sexy models, each of whom is driving their own Corvette against the player's.
Monster Truck racer Madusa, who drives the machine of the same name
Reiko Nagase was portrayed this way in the third Ridge Racer game, at the very least as more than just eye candy. Later, the trope would be shown in full with Rena Hyami, ambulance driver turned racer.
Ash, Emily, Kate, and even Heather from Misfile, all into street racing.
Kallen's Guren in Code Geass came equipped with a motorbike-like pilot seat, for no apparent reason other than to invoke this trope. The Guren's mass-production derivative, the Akatsuki, has the same cockpit. Plenty of female pilots too.
The same goes for Faye from Cowboy Bebop and her fighter ship.
Kitori from the Area 88 TV series flies as well as any man at the base. And she lives longer than most.
Cabin fits this role nicely in Dino Attack RPG. A hardcore, attractive, and fully qualified helicopter pilot whose practically second in command when it comes to taking action in the skies... at least until the war ended.
Hannah Reitsch, German test pilot and combat flier.
Lidya Litvyak, Russian air combat ace.
Kim Campbell (not to be confused with the former Canadian prime minister of the same name) not only flies A-10 Thunderbolts in combat, but managed to land one with total hydraulic failure after taking a hit over Iraq (a Crowning Moment Of Awesome, regardless of what one thinks of the war itself).