He Really Can Act: George Clooney shows impressive range as Seth, making him sympathetic, honorable and charismatic while still making it clear just how dangerous he is. Quentin Tarantino also does great work as Richie, excelling at making him unnerving and believably dangerous yet oddly pitiable.
Because of the Halfway Plot Switch and the fact that pre-Mexico was all talk, talk, talk while Mexico was action, action, action; film-buffs everywhere instantly declare that it was obvious that the first part was directed by Quentin Tarantino and the second part were directed by Robert Rodriguez. The duo would end up doing exactly that in their film(s) Grindhouse.
In the Japanese dub of the film, Nobuyuki Hiyama voices Scott Fuller, who was one of his first roles as a voice actor. A decade later, he ends voicing Kouta Hirano. The hilarity came with the fact Hirano hunts zombies and Scott is turned into a vampire and later killed off. Extra hilarity he also voiced vampire lord Demitri Maximoff in Darkstalkers few years later.
Pete Bottoms, the ill-fated store clerk in the beginning of the movie, says to Seth that he's acting natural and quips "I should win a fucking Academy Award for acting natural". John Hawkes would later get nominated for Winter's Bone, though.
Memetic Mutation: The pussy monologue and Salma Hayek's snake dance are both extremely memorable scenes, and as such, they've both taken on a life of their own beyond the movie.
Moral Event Horizon: Richie's rape and murder of his and Seth's hostage. According to Quentin Tarantino, he had already crossed it with his first rape (in every movie he's directed and/or written, he kills off every character who's a rapist, particularly if the rapist is his character).
The scene in which Richie sucks Santanico's whiskey-soaked bare foot should be as sexy and hypnotic to the viewer as it is to Ruchie himself, but it just becomes hilarious and/or awkward if one is familiarized with Tarantino and his infamous foot fetish. Any suspicions that Tarantino wrote that scene (or even worse, the entire film) for himself are probably right.
There's also Seth's penchant for threatening to shoot people in the face. Given what infamously happened in Pulp Fiction, it's clear that Tarantino isn't fooling anybody with that.
Nausea Fuel: Among the different types of pussy at Titty Twister are "bloody, horse, and chicken."
This is the reason why the Mexican Spanish dub changed this line to women instead, which ironically makes the original line even more disturbing.
Salma Hayek has all of ten minutes screentime half way through the movie and is one of the most memorable things in it. Her face is prominently on the cover of the box, for God's sake.
John Hawkes as a store clerk who is held hostage by the Geckos in the opening scene.
They Wasted a Perfectly Good Character: Santanico Pandemonium, Chet Pussy, Razor Charlie and the bouncer are all vampires with distinct personalities and have a considerable leadership presence in the vampire swarm living at the Titty Twister. However, they're all killed within minutes of showing their true colors and most of the vampires fought from that point forward are just interchangeable mooks.
They Wasted a Perfectly Good Plot: For the rare first-time viewer who didn't know about the Halfway Plot Switch, the first half of the film sets up a premise and conflict that, had it been truly followed up on, could have been a lot more interesting than the mindless gore-fest that the film turns into.
Does the fact that Santanico has being tampering with Richies mind make him less of psycho monster than his movie counterpart? Or does it make him worse?
In the episode "Opening Night", Seth stakes a culebra that has been showing a great amount of interest in Kate. Does he do this out of Big Brother Instinct or out of jealousy? Or does he just want to kill somebody to vent his frustrations?
Anti-Climax Boss: The Nine Lords of Night are built as the Greater Scope Villains of the show and a bigger threat than Santanico, yet their leader Amancio Malvado is killed off before Season 2's climax and the remaining Lords are all murdered by a couple of mind-controlled minor vampires.
Santanico. Some fans are constantly clamouring for her return; others say, arguing that her arc ended as of season two, that she has no real reason to be hanging around anymore.
Broken Base: Whether the show should have a Season Four. Some fans are clamoring for more, while others think Season Three's ending was perfect for the series.
Complete Monster: Amaru, Queen of Xibalba, who once enslaved the entire vampiric Culebra race, takes the chance to jump into the dying body of Kate Fuller when her seal is weakened. Slaughtering the remaining Culebra Lords save one survivor, Amaru proceeds to torture and kill multiple culebras and humans, even murdering the human lover of Kisa (aka Santanico Pandemonium) just to hurt her out of belief that Santanico has no right to call herself a queen. Amaru spends the remainder of the season sending horrible demons after her enemies that cause incredible amounts of pain and suffering, and tries to kill Kate's loved ones to force her to watch as a prisoner in her own body. Causing several more massacres, Amaru regains her own original body, her influence driving an entire nearby town mad with the inhabitants of a hospital slaughtering one another. Amaru soon reveals her intent is to drag the entire human and Culebra races into hell so she can torture their souls forever in revenge for her sealing.
Dhampyr: Technically, except from the Nine Lords, all of the Culebras are this, As the Nine Lords are born Culebras, demons from Xibalba, whereas other Culebra shown is turned from them making them hybrids instead of full bloods.
Draco in Leather Pants: While not as villainous as they first appear, the Gecko brothers certainly qualify all the same. Richie in particular has gotten a lot of leeway since Season One. Again, while he does have his moments of empathy (along with the fact that most of his more villainous actions were directly caused by Santanico messing with his head), those moments should not cloud the fact that he's still a hardened career criminal who's more than willing to spill blood if he has to.
Amaru and Brasa, what with the two of them being Hell-Bent for Leather and prone to speaking in sultry, deep raspy voices.
Carlos and Narciso qualify as well.
Fan-Preferred Couple: Seth and Kate, which is around twice as popular as the next biggest pairing of Richie and Kate.
Growing the Beard: The second season in general has better pacing, better action, more lovable characters (Uncle Eddie, anyone?), a story that's exciting and unpredictable and a hell of a cliffhanger.
Harsher in Hindsight: All those vampire strippers you see in the movie? The tv series reveals that they used to be innocent young women who were kidnapped and sold to the Greater Scope Villains to create a vampire army. Yes, even Santanico Pandemonium.
Launcher of a Thousand Ships: Kate's been paired with pretty much everyone by the fandom. Her actress (Madison Davenport) ships Seth/Kate - amusingly, she seems to flirt with DJ Cotrona (Seth) quite often on Twitter, causing fan speculation to go wild about their relationship. Cotrona's penchant for Romance on the Set only adds fuel to the fire.
Rescued from the Scrappy Heap: Scott was dangerously close to being a Scrappy, but has definitely been rescued in season three, thanks to Brandon Soo Hoo proving his acting chops were better than the melodramatic fluff he was given in season two. His getting to show off his martial arts skills definitely helped.
Rooting for the Empire: The Gecko brothers are certainly not good people, but nearly everyone still roots for them. Freddie Gonzalez is working against them, but it's easy to support him as well.
The Scrappy: As of season three, it's Dakota. Her appearance in the show seems a little unnecessary, and she did almost nothing of value while being played by an actress who was very stiff, awkward and had next to no chemistry with the rest of the cast.
They Wasted a Perfectly Good Character: The shows has a pretty bad habit of creating very likable characters and extremely cool monsters...only to kill them off before the season's over. While you could argue that the deaths of Earl Mgraw and Uncle Eddie were necessary to the plots of their respective season, there was absolutely no reason to kill off characters like The Regulator and Burt.