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Dora Márquez
Played by: Isabela Merced, Madelyn Miranda (young)
The protagonist of the film, and a young explorer.
- Adaptational Badass: Dora was more about traveling from one place to another, while there was sometimes a little bit of peril most of it was about Free-Range Children. Her teenage self here is introduced escaping a herd of stampeding elephants while explaining their specific subspecies via livestream, and is more of an Adventurer Archaeologist.
- Age Lift: In the original cartoon, she was a 7-year-old, and in Dora's Explorer Girls she was a pre-teen. The prologue shows Dora and Diego at six years old, while most of the movie takes place ten years later. Dora is now a teenager, attending high school.
- Composite Character: This version has Diego's way with animals and the jungle.
- Crazy Survivalist: She's definitely this way compared to other high schoolers. Her backpack has flares, tents, portable generator and anti-venom.(Dora produces a Rambo-style bowie knife)
Sammi: "Oh look, Dora brought a knife on the field trip, everyone!" - Embarrassing Nickname: Dorka
- Fish out of Water: Having spent years exploring the jungle with her parents, she has a little trouble adapting to city and high school life.
- Le Parkour: As someone used to wilderness, she can maneuver rough terrain quickly and efficiently.
- Homeschooled Kids: Was raised by her professor parents so that while she may not know a lot of the basics of American high school social etiquette, she's highly intelligent in several subjects.
- Killer Yo-Yo: She has a yo-yo which she considers to be her deadliest weapon. She later proves it when she knocks out a mercenary with it.
- Nice Girl: Is friendly and cheerful towards everyone she meets even if they start off rude and mean to her.
- No Social Skills: Since she spent most of her life exploring in a jungle, she has no idea how to act in school.
- The Pollyanna: Throughout the film, her attitude tends to be very optimistic, even in great danger.
- Prefers Rocks to Pillows: On her first night in America, she sleeps in the garden rather than her bedroom.
- Raised by Wolves: Played with. She's not entirely familiar with the high school crowd but this results in being overly friendly and enthusiastic, rather than lacking basic social awareness. She later admits she knows kids think she is a dork but refuses to not be herself.
- Speaks Fluent Animal: Boots communicates largely via noises provided by Dee Bradley Baker, but, in the face of danger, can talk to Dora in the voice Danny Trejo. She's as surprised as we are. At this point, the other characters only hear Dora and Boots making monkey noises at each other.
- That Reminds Me of a Song: Is ridiculously prone to this.
Diego Márquez
Played by: Jeff Wahlberg, Malachi Barton (young)
Dora's cousin, he was really close to Dora when they were young until his mother got a new job and they had to leave the jungle to live in Los Angeles.
- Adaptational Dye-Job: Sports a buzzcut as a teen, although his hair looked similar to his animated counterpart when he was younger.
- Adaptational Jerkass: In the original series and the TV show he was every bit as nice as Dora. In here, he yells at her and calls her a weirdo, although he does gets better. To be fair, he was best friends with Dora when they were six years old, and only Took a Level in Jerkass after growing up into a 16-year-old trying to be more responsible and mature, and only wanting the best for his cousin.
- Animal Motif: Has a jaguar plush in the flashback at the beginning and he notices a jaguar statue in one of the Parapata puzzles, both in reference to Baby Jaguar from his series.
- Jerk Jock: Plays soccer, and starts out rather aloof, apathetic, and shallow. He advises Dora not to hang out with geeky Bully Magnet Randy (she thankfully disregards his warnings and becomes friends with Randy), tries to keep himself from associating with her to avoid embarrassment, and then later yells at her for being "weird". He fortunately gets better in the end, embracing his cousin's weirdness and befriending Randy.
Boots
Voiced by: Dee Bradley Baker (vocal effects), Danny Trejo
Dora's pet monkey.
- Beneath Notice: Boots utilizes the fact he is a wild animal to distract the mercenaries from being suspicious of the noises coming from the group.
- Suddenly Voiced: He mostly communicates with monkey noises, but speaks English at one point to give Dora a pep talk.
- Vocal Dissonance: He communicates largely via noises provided by Dee Bradley Baker, but, in the face of danger, can talk to Dora in the voice of Danny Trejo. She's as surprised as we are.
Swiper
Voiced by: Benicio del Toro
The kleptomaniac vulpine nemesis of Dora.
- Adaptational Badass: While somewhat downplayed, he's so much more competent and threatening than in the show. Given that he's the mercenaries' right hand fox. To top it off, this time he does not concede defeat to the whole "Swiper, No Swiping!" thing (or at least not as easily).
- Adaptational Curves: While still pretty lean, he has more of a build than in the cartoon.
- Adaptational Villainy: He's much more ruthless in this film, and works for mercenaries. He even averts "Swiper, No Swiping!" as the icing on the cake.
- Foul Fox: An actual threat in this movie; he's affiliated with mercenaries and he helps them capture Dora's parents.
- Karma Houdini: Played with, while he did suffer some amount of punishment, he gets away scot-free in the end and is the only merc who did not face punishment from the Incan. The closest he gets to a comeuppance in the end is him being caught red-handed trying to steal Cole and Elena's Inca statue, and this time, he listens to their "Swiper, No Swiping!" order.
- Real After All: At first, we're led to believe that he merely exists in Dora's imagination. Then he shows up in the real world, working for mercenaries.
Sammy Moore
Played by: Madeleine Madden
- Academic Alpha Bitch: She antagonizes Dora for one-upping her in class and is one of the most powerful students at Silverlake High School, although widely disliked. She gets better.
- Straw Feminist: There are admittedly a couple of moments like this, particularly in which she deflects from Diego genuinely calling her out for being bossy by accusing him of being a misogynist.
- Took a Level in Kindness: She starts off the movie quite unpleasant but thanks to her adventure with Dora becomes a nicer person.
Randy Warren
Played by: Nicholas Coombe
- Amazon Chaser: After watching Dora Le Parkour after Swiper to retrieve the map and then grab a (slowly) moving bi-plane blade to send Swiper flying, he has this look of awe and says "she's amazing".
- Deadpan Snarker: Surprisingly, he gets a few quips in.Randy: For the first time, I'm actually feeling really good about how things are- (the group falls through the ground into an aqueduct) ...ignore what I just said.
- Token White: Randy is the only prominent non-Hispanic of the cast (the few mercenaries seem slightly more diverse), and even does not understand Spanish, having taken Mandarin.
- Wrong Genre Savvy: While the group are stuck in a temple, Randy pulls a lever in hopes of completing the "jungle puzzle" only to end up flooding the cave.
Alejandro Gutierrez (SPOILERS)
Played by: Eugenio Derbez
- Big Bad: He's the real leader of the mercenaries.
- Death by Materialism: Subverted. His obsession with getting the treasure of Parapata leads him to trigger a trap that drops him down a flaming pit, with Dora pointing out his greed caused this. However, he manages to grab the edge and survive. Instead, he is made a prisoner of the Guardians of Parapata.
- Evil Is Hammy: Once his villainy is revealed, he's clearly having fun in his performance.
- Fate Worse than Death: Alejandro's final fate is this. He is imprisoned in the temple while chained to a table where he is force-fed bugs. This would be bad enough on its own, but the soldiers that are watching over him sing, "You'll be here for a thousand years" suggesting that Alejandro has been made immortal in some way.
- Faux Affably Evil: Pretends to help Dora but is later revealed to actually be the leader of the mercenaries and Swiper's boss.
- Obfuscating Stupidity: His entire goofball and Cowardly Lion act was just that...an act. He's actually the leader of the mercs that kidnapped Dora's family.
- Walking Spoiler: Just reading his other tropes alone spoils his entire character.
Elena Márquez
Played by: Eva Longoria
- Named by the Adaptation: She was known simply as “Mami” in the cartoon (the GoAnimate community had given her the name "Veena"), but is given the name Elena here.
Cole Márquez
Played by: Michael Peña
- Manly Tears: He's prone to this, notably towards the end when Dora decides to navigate high school over exploring with her parents.
- Named by the Adaptation: He was known simply as “Papi” in the cartoon, but is given the name Cole here.
Christina X
Played by: Natasa Ristic
- Bait the Dog: While posing as a museum guide, she sympathizes with Dora after overhearing how she and her group want to win the scavenger hunt, and she deliberately leaves a door ajar so Dora can go into the basement to find some pieces not yet on display. Turns out this was to lure Dora into a kidnapping trap.
- The Smurfette Principle: She's the only female mercenary in the group.
- What Happened to the Mouse?: Is not seen with Alejandro in the Incan prison. Leaving what happened to her unknown.
Viper
Played by: Christopher Kirby
- Butt-Monkey: Among the mercenaries. When they think he got spooked by a monkey (Boots covering for Dora and the others), they make fun of him for supposedly being afraid of monkeys. Later on, Dora knocks him out with a yo-yo, and the mercenaries talk as though this has happened before and don't seem concerned.
- Scary Black Man: Very intimidating, though ultimately subverted when he proves to not be much of a threat once the kids get their hands untied.
- What Happened to the Mouse?: Is last seen being knocked out with the yo-yo, so what happens with him remains unknown as he's not with Alejandro in the Incan prison.
Powell
Played by: Temuera Morrison
- Too Dumb to Live: He doesn't die from it, but while standing guard in a dangerous jungle, he literally offers his hand to an unidentified frog. Said frog promptly sticks him with a paralytic poison by touching his hand.
- What Happened to the Mouse?: Last seen getting poisoned by the golden poison frog. As he's not in the prison Alejandro is sentenced to, his fate, much like that of Viper and Christina X's is never shown. Of course, given that Swiper recovered from being knocked out by the frog's poison, it's likely that he did too.
Kawillaka
Played by: Q'orianka Kilcher
- Reasonable Authority Figure: She is willing to allow Dora and her friends a go at solving the treasure's puzzle after being convinced that they are not part of Alejandro's group.
