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Examples of Big "NO!" in Western Animation


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    A 
  • A Kind of Magic: Cindy lets out a Big No in the episode "Meet the Parents" when her Love Interest Leo faints from sniffing her dirty socks.
  • Ace Lightning: The characters in this mixed media show are based on superhero stereotypes, so this was going to show up sooner or later — except that it's not one of the CGI characters that do it — it's a human, in the last episode, when he thinks Ace has been shot.
  • The Action Man (2000) episode "Tangled Up in Green" has Professor Gangrene yell a Big No after he is tricked into destroying Action Man's plant clone.
  • The Adventures of Jimmy Neutron, Boy Genius:
    • In "Hall Monster", Jimmy gets one when he is selected to be Lindbergh Elementary's new hall monitor after the previous one, Chuck Lester, moves to Greece.
    • In "Love Potion", Jimmy screams after realizing what has he done.
    • Jimmy gets another one in "The Tomorrow Boys", when he travels to the future and he discovers that his future self married Cindy. After one commercial break, we find out he is still screaming, while his friends have some choice words.
      Carl: Wow, you just screamed for 4 minutes, Jim.
      Sheen: I'm both impressed and disturbed.
    • Sheen lets out one in the interstitial shorts after his Ultra Lord action figure gets stuck in a tree. Also lets one out in "Ultra Sheen" when he witnesses Ultra Lord's defeat at the hands of Robo-Fiend inside the video game.
    • Sheen lets out another in "Crime Sheen Investigation" after Ultra Lord has been stolen.
    • Hugh does this in "Attack of the Twonkies" when Judy takes Flippy from him and throws him to Jimmy so he can power up his rocket and send the giant Twonkie back into space.
  • Happens quite a lot on Adventure Time.
    • In "Wizard Battle", Finn has to win a contest in order to win a kiss from his love interest, Princess Bubblegum. When one of the other contestants almost wins, he lets out a Big "NO!" that reaches the level of a sonic scream.
    • In “Dad’s Dungeon”, Jake does this in slow motion while stopping Finn from eating one of the apples that the Fruit Witches had to offer.
    • Finn also lets one out in "Mystery Train" when he sees that Jake has seemingly been killed by the Conductor.
    • Me-Mow in "Jake vs. Me-Mow" when she learns of Jake's shapeshifting as he uses it to thwart her poison.
    • Finn let's out one of these near the end of "Mortal Recoil", when Nurse Pound Cake says that Princess Bubblegum is okay, but there were some complications.
    • Most notable example is The Lich in the season 4 finale. Finn is about to destroy the Enchiridion, so he lets out a Big "NO!". But then, as it turns out, he was counting on Finn destroying the Enchiridion after he did so, which is what allowed the Lich to access the Time Room and attempt to wipe out all life... oops.
  • The Amazing World of Gumball:
    • Several from Gumball, such as when he's unsuccessful in attempting to learn Darwin's secret in "The Secret" and sees Darwin kissing Penny in "The Dream".
    • Darwin in "The Dress" when he thinks Gumball's female alter ego, which the latter had substituted with a balloon, died from flying into the sun.
    • Richard gets a few, such as in "The Painting" when he's told he has to get a job, and in "The Job" when he gets a job as a pizza delivery boy and loses it because he was caught eating the pizza. The most notable example is during a flashback in "The Wand", where as a child, he was told that magic wasn't real and did one that lasted for 15 years (though this is contradicted by later episodes).
  • Used in American Dad! by either Stan, Francine or Hayley when they believe Steve and Roger were killed by a lightning-struck treehouse. Rather effective due to how strangled and broken it sounded. Steve also gets a particularly good one.
    • Stan has had a couple of these.
  • Aqua Teen Hunger Force:
    • Meatwad does this often. Examples include "Balloonenstein" when his newfound electrical powers cause his dead pet squirrel's corpse to start smoking, "Ol' Drippy" when Drippy gets hit by a truck while pushing Shake out of the way, "Circus" when Meatwad hears that Shake ate Inside-Out Boy, and "The Cloning" when the cloned TV shows him disturbing footage of Frylock murdering singing candy people with a candy cane gun and then aiming it at the Meatwad on the screen.
    • Shake gets one in "Boost Mobile" when his giant cellphone friends are crushed to death by a boulder.
  • Arthur:
    • Used in an Imagine Spot scene in the episode: "D.W. The Picky Eater". Arthur was not allowed to go to outer space without his sister D.W.
    • Fern gives one in "Phony Fern" when her cellphone gets flattened by a steamroller.
    • Buster screams this in "Happy Anniversary", after realizing he ate a sandwich thinking it was his homework.
    • In "Unfinished", Arthur let's out one of these when he finds out that the ending pages from the copy of 93,000,000 Miles in a Balloon that he got from a woman in Shelbytown had them scribbled all over with crayons, courtesy of her children.
  • Avatar: The Last Airbender:
    • There's a slow-motion one from Zuko in the Grand Finale when Taking the Bullet for Katara. Cue "must run in the family" jokes...
    • It really does; Iroh has a (much less dramatic) one when he sees June get hit with her Shirshu's paralyzing tongue.
    • Sokka has one in "The Desert", right before Aang creates a giant mushroom cloud.
    • Sokka also lets out one in "The Cave of Two Lovers", when he finds he's trapped in a cave with the nomads.
    • Parodied in the Who Would Want to Watch Us?? episode when Actress Aang lets out a nice 9 second "NOOOOOOOOOOOOOOOO!" as she "dies".
    • Katara has one in the Puppetmaster when Hama nearly forces Sokka and Aang to kill each other using Bloodbending.
    • The Legend of Korra (Sequel Series of Avatar: The Last Airbender):
      • Unavaatu pulls out one while being spirit bent and destroyed by Korra.
      • Korra also has one of these in the Book 1 episode "Endgame" when she sees that Mako is about to have his firebending removed by Amon. Even though she has already had her own powers removed, her desperation to save Mako leads to her finally unlocking airbending.
  • The Avengers: Earth's Mightiest Heroes has Kang the Conqueror say this as he gets banished from the Avengers' time period.
    B 
  • In Babar, Babar pulls one when he discovers his mother's dead body.
  • Batman: The Animated Series:
    • "Pretty Poison" and "Eternal Youth", each near the end, have the villainess Poison Ivy saying this trope.
    • Subverted in "Two-Face".
    • "His Silicon Soul", near the end, has the cyborg Batman saying it after the real Batman seemingly fell to his death before Cyborg Batman committed suicide. Then it's revealed that Alfred sees a light below which is Batman himself, who survived.
    • Used in "Mad Love" by Harleen Quinzel after The Joker gets captured again and taken away from her which helps to show us her Sanity Slippage.
  • In Batman Beyond, Derek Powers accidentally inhales a big gulp of his own nerve gas, and in the same second it takes him to realize what has happened to him, makes an appropriately horrified scream to this effect.
  • Beavis and Butt-Head - Beavis often screams out a lengthy "AAAAAAAAAH NOOOOOOO!!!" if he's distressed, often by a video that sucks.
  • Bob's Burgers:
    • "Tina-Rannosaurus Wrecks" features a very deadpan version, when Tina becomes complicit in insurance fraud, and imagines going to Hell-Jail.
      Tina: What's for lunch?
      Demon Lunch Lady: Your lies!
      Tina: Nooooo! That's what we had yesterday.
    • In "Ears-y Rider", Louise lets out an anguished "NOOOOOO!" when it looks like her beloved rabbit-ear hat has been thrown in the incinerator.
    • Bob lets out a Big "NO!" in "Roamin' Bob-iday" when his family locks him out of the restaurant in order to force him to take a day off.
    • In "The Gene Mile", Gene manages to combine a Big No, a Big "WHY?!", and a Skyward Scream during the flashback to the last "Free Scoop Friday" at the ice cream parlor. By the time the Belcher kids got through the huge line, there was nothing left but "rum raisin sorbert".
  • The Bonkers episode "In the Bag" has Lucky Piquel say this trope when both Bonkers and Lucky are chasing the bag in his police car only to find that the brakes are missing.
  • In Brother Bear, Kenai lets out the distant, bird-disturbing version of a Big No when he discovers he's been transformed into a bear.
  • Breadwinners: Used as a running gag by our protagonists.
    "NOOO! Anything but that!"
  • Bump in the Night:
    • In the episode "Night of the Living Bread", the Bread hollers "NO!" right before Mr. Bumpy knocks him down by flinging peanut butter at him.
    • The Cute Dolls scream "No!" at the end of "Party Poopers" after Mr. Bumpy promises to go to every party they ever throw.
  • Buzz Lightyear of Star Command: The episode "Plasma Monster" has Booster react this way when Plasma Boy's girlfriend tells him they can still be Just Friends.
    C 
  • In an episode of Camp Lazlo, Scoutmaster Lumpus does it when Slinkman says that he can earn his "Handy Helper Badge" if he can...gasp!...help someone!
  • Captain Planet and the Planeteers:
    • "The Conqueror": After Captain Planet tosses Zarm's spaceship into an inactive volcano, Zarm tries to press a button (presumably to launch nuclear missiles and start a war, as he announced not long earlier), muttering that Captain Planet hasn't yet gotten rid of him. Moments later, Gaia makes the volcano erupt, causing Zarm to yell a long, horrified "NO!" as his ship gets launched into the air like a ball.
    • "Don't Drink the Water": When Skumm and Blight criticize how poorly Plunder's plan is going, Plunder says that it doesn't matter if Gi escaped, because he already killed Captain Planet, Kwame, Ma-Ti, and Linka. Wheeler screams his denial. Two henchmen have to hold him back as he lunges at Plunder and threatens him.
    • "Mind Pollution": Skumm's last scene ends with a howl of "No!" as he realizes that he ate some of his own drug and is doomed to suffer the same things he inflicted on Linka and the people of Washington D.C.
    • "Wheeler's Ark": Played for laughs. The Planeteers are bringing homeless endangered animals to Hope Island in a misguided effort to protect them, so Gaia eventually tells them to take the animals back where they belong. The kids don't seem to get the idea, though, and instead turn up with even more endangered animals. When Linka says they can try to return the animals again, Gaia instantly shouts a horrified "NO!"... and laughs it off just as quickly, somewhat embarrassed.
      Gaia: Please, no, no, no. I'll handle it this time.
  • The Cartoon Sushi short "The Mayfly Has a Lifespan of 2 Hours" ends with the Mayfly screaming "No!" when he screws up his attempt at a one-night stand by ejaculating prematurely and discovers that he only has a few seconds left to live.
  • Celebrity Deathmatch: One episode has Elizabeth Hurley and Anna Kournikova fighting atop a cage filled with horny teenage boys. Hurley manages to pound Kournikova into the cage, followed by a series of limbs and carnage flying from the top. Hurley assumes that the boys have ripped her opponent to pieces, until a pair of hands grab at her feet from beneath.
    Hurley: "Mr. Lane, get these boys off me!"
    Kournikova: "Guess again, Hurley!"
    Johnny Gomez: "Anna Kournikova is alive!"
    Nick Diamond: "She must have ripped those boys to pieces!"
    Hurley: "NOOOO!"
    • Johnny Gomez pulls one of these after his co-host, Nick Diamond, is thrown from the announcer's booth after it is damaged by an air-born Roger Ebert.
    • Nick also does one of these during the Congressional Hearings episode, after Senator Ted Kennedy sentences him (and Johnny) to no more than 10 hours of community service.
  • Chowder:
    • In "The Meach Harvest", Mung Daal does two, one in present and one in a flashback.
    • In "Chowder's Catering Company", Chowder gives one after Shnitzel dumps the furry creatures that Chowder was feeding his dishes to in the garbage.
    • In "Banned from the Stand", when Gazpacho goes mad with paranoia and begins banning everyone from his produce stand, a guy that he bans from the bathroom does this when Gazpacho clarifies that it's from every bathroom.
  • Code Lyoko:
    • When it hits the fan, expect any of the Lyoko Warriors (Jeremie or Aelita more) to yell a huge one.
    • When XANAfied, William will yell one if his "Throw Aelita into the Digital Sea" plan fails.
  • Codename: Kids Next Door does this quite a few times:
    • From Numbuh 2 in "Operation: F.A.S.T.-F.O.O.D." when Numbuh 3 is sent to buy fast food for her birthday, but the restaurant turns out to be a front for an operation where children are literally made out of burgers and sold to hungry sharks. While he and the rest of the team chase the family of sharks when they learn Numbuh 3 is about to and almost (due to escaping at the last minute) become their dinner, Numbuh 5 tries to fire the fishing hook at their car before it drives back into the ocean, but the shot barely misses at the last possible moment.
    • Future Madame Margaret from "Operation F.U.T.U.R.E." does this after Sally Sanban, the granddaughter of Numbuh 3 destroys her time machine after Future Numbuh 4 uses it to travel back in time to the present day Kids Next Door and stop her present self's evil plan.
    • From the vampirized Numbuh One near the end of "Operation: L.O.C.K.D.O.W.N." after he and the other three operatives were turned into Spank-Happy Vampires and about to spank Numbuh Five for using the Quadruple Emergency Bypass Code to lift the lockdown, only for the morning sun to rise, ending the lockdown and weakening the vampires just in time. After the other three vampirified operatives let out screams of pain due to the sunlight, he lets this one out, seconds before the scene transitions to Count Spankulot's cell.
    • From Heinrich Von Marzipan near the end of "Operation: C.H.O.C.O.L.A.T.E." (after he is turned into a chocolate monster after falling into the chocolate volcano in "Operation: R.A.B.B.I.T."), when fighting Numbuh Five in a spacecraft and she gets close enough to the sun to make his chocolaty body melt. In this case, it's a Big "NEIN!", but the meaning is still there, as "Nein" is German for "No".
    • Numbuh Two again in "Operation: A.M.I.S.H." when he has to lay low in Sector A (Amish country) when the mysterious Splinter Cell is abducting Kids Next Door scientists, and he's not allowed to use any technology, not even to defend himself.
  • Cow of Cow and Chicken did it in an episode while jumping to catch a coin. In slo-mo. With the entire world slowed down as well.
    D – E 
  • Danny Phantom:
    • When Danny's family and friends (and teacher) are about to be blown up to their doom in an alternate timeline, Danny cries one out, complete with slow motion movement.
    • Vlad later gets one in another episode when his perfect clone is destroyed by Danny and his Opposite-Sex Clone. For just one word, it's brilliantly acted.
  • Dante's Inferno: An Animated Epic - Dante lets out a pained and enraged (and kinda narmy) big no as he beats on the Gates of Hell that separate him and his wife's soul.
  • Parodied in the Musical Episode of Daria. Daria gives a Big No as Kevin accidentally locks the four of them out on the roof.
  • Dave the Barbarian is prone to parodying this. It has often been used over decidedly minor things or for humour's sake. Such as this:
    Dave: Is my nose clean now?
    Rejected Lula: NOOOOOOOOOOOOOOOOOOOOOOO!!!
    Dave: I was only asking...
  • DC Super Hero Girls (2019) has occasionally used the trope of a character screaming "NO!" as a reaction to situations that greatly distress them.
    • The short "#Booked" ends with Supergirl doing so after learning from a text sent by Carter Hall that she missed the concert she wanted to go to by the time she was done organizing the library's books.
    • The episode "#BurritoBucket" has Batgirl do so when she sees that her difficulties in balancing her work life and her duties as a superhero have resulted in the bank robbers she tried to stop getting away with their loot.
  • Dexter's Laboratory:
    • Happens quite a lot. One time in particular, when Mandark forces Dexter to shut down his laboratory.
    • In "Jeepers Creepers, Where is Peepers," the transforming Peepers yells this when he sees Dexter and Koosalagoopagoop being attacked by Hokochu before breaking out of his container.
    • The Ice Cream Man, after Dexter asks if he's got change for a hundred. This is after the Ice Cream Man explains that he hates Dexter for paying in pennies.
  • The Donkey Kong Country episode "Buried Treasure" contains this exchange when Klump's video conference interrupts K. Rool's computer game:
    Klump: General Klump reporting, King K. Rool sir! Y'read me, your high 'n mightyness?... Looking a little pale there, chief!
    K. Rool: NNNNNNNNNNOOOOOOOOOO!!!!!!!!!!!!!!!!!!! (smashes the computer)
    Klump: (calling on the phone) Chief? You there, chief? Seems we had some sort of interference.
  • Dragamonz: Fyra lets out a pretty heart-wrenching one of these when it appears that Dax has been defeated and captured by a brainwashed Boaragon.
    • Grimserver also lets one of these rip when he is finally destroyed for good.
  • Parodied throughout the Drawn Together episode "Terms of Endearment", in which Captain Hero screams "Nnnooooooo!!" at least 7 or 8 times. At one point (as the camera pulls back... and back... and then back some more, to reveal just how big a problem he's facing) Hero screams the Big No three times in a row, with dramatic pauses in between.
  • Eek! The Cat lets one out in the episode "Cape Fur", when he's being dragged off by JB for punishment after he hears about the Killer Rabbit on the news.
  • In El Tigre: The Adventures of Manny Rivera, Manny lets one out after inadvertently killing his school's zebra donkey mascot by feeding it bananas, which were poisonous to it.
    F 
  • Parodied in The Fairly OddParents! a few times:
    • Played straight in the The Fairly Odd Parents Shadow Showdown video game; Timmy shouts it after his TV breaks in the introduction, and again upon seeing he's missed the Crash Nebula season finale at the end.
    • And parodied in "Pixies Inc", where, after being foiled, the Pixies let out a big no—with no emotion.
    • Cosmo and Jorgen in "Cosmo Rules", when they learned they're cousins. Their Big "NO!" lasted nearly 24 hours as the scene showing the sun setting and rising again suggested and then Jorgen fainted and Cosmo added ..."WAY!" Cosmo was enjoying the idea and even planned cousin-ly activities for him and Jorgen. Albeit not all of them were fit for male cousins.
    • Crocker in "Formula for Disaster" at the end when he is trapped on a bus headed to Cincinnati he yells "NOOOOO! Not Cincinnati!" A sign saying "Welcome to Cincinatti! Crocker free since 1983" may have something to do with it.
    • Chester also did this in "The Boy Who Would Be Queen:" when A.J. fell in love with Timmy's girl form "Timantha."
    • On the Oh Yeah! Cartoons short "The Temp", Jeff gives off one as he's dragged back to the North Pole, with his fellow elves telling him to "Be one of us!"
    • In "Boys in the Band", Vicky gives one after she finds out Chip Skylark, who she was about to marry, was not only broke, but in debt.
    • In "Fairy Idol", Tilly screams one, coupled with Eat the Camera, when he finds out Cosmo and Wanda have quit.
  • In F is for Family, Frank has the reaction upon discovering Bill witnessed him and Sue having sex.
  • Family Guy:
    • One episode had Peter be really upset over the loss of his pet parrot.
    • Peter again in "McStroke" when his mustache burns off.
    • Quagmire does this after realizing Brian had sex with his transgender father.
    • In the "Stewie Kills Lois/Lois Kills Stewie" duology, Peter shouts one to the sky after Joe solemnly informs him that Lois is dead.
  • In the Freakazoid! episode "House of Freakazoid", Freakazoid is pressured to pursue a villainess through a sewer, but repeatedly refuses because he hates the smell of poo gas.
    Freakazoid: NO! A big N, followed by a little O: no!
  • Fanboy and Chum Chum:
    • In "Dollar Day", Fanboy shouts it when Chum Chum spends their only dollar on the Chimp Chomp machine.
    • In "The Hard Sell", Oz screams one when Fanboy is about to give his Crabulous figure to Lenny.
    • In "Digital Pet Cemetery, Chum Chum gives one when he sees Fanboy overfeeding Scampers.
    • By FB and CC in unison in "There Will Be Shrieks" when they realize they cannot taste candy anymore.
    • In "Back From the Future", Lenny screams one as the Alternate Universe FB and CC torment him with their "More!" chant.
    • Oz screams three of them during his flashbacks in "Rattleskunkupine!", combined with Skyward Scream, when the titular creature makes off with his food. He screams a fourth one when he hears the creature escaped.
    • At the end of "Slime Day", FB and CC shout one in unison — coming out as "Know!" — when they see the giant bucket of slime pouring down on them.
    • One by Kyle in "Speed Eraser" when Fanboy is about to unintentionally erase his head with the enchanted eraser, followed by another when he erases his wand.
  • Gary from Final Space does this, combined with Say My Name, whenever someone he cares about (especially Mooncake) puts themselves in danger
  • Foster's Home for Imaginary Friends: At the end of the Grand Finale "Goodbye to Bloo", after Mac reveals he's moving into Louise's old apartment right next door to Foster's, it was soon revealed the place that Louise is moving to does not allow imaginary friends; thus her imaginary friend, the annoying and psychotic Cheese, has to move into Foster's, and this is everybody's reaction.
  • Many in Futurama. In particular, being a parody of melodramatic acting, Calculon the robotic soap opera star is prone to do this sort of thing a lot.
    • "Bend Her" has two in one episode. Calculon is being interviewed on a talk show with Coilette as another guest (who is Bender who changed his robotic sex and invented his own country to win multiple fembot Olympic medals). When asked to set the audience up for the clip they are about to show from a new episode, Calculon responds "No, I think it's self-explanatory." The clip shows Calculon screaming "NNNNOOOOOOOOOOOO!" (while a pirate inexplicably cooks burgers on a barbecue behind him). After the clip, Calculon nonchalantly replies, "Funny story: The script called for me to say 'yes' but I gave it a little twist." Afterwards, "Coilette" ends up engaged to Calculon but has to call the wedding off before the sex change becomes permanent. After... she fakes her death of at what would have been her wedding to Calculon...
      Dr. Zoidberg: (in the middle of eating and nonchalantly) I'm a doctor. He's dead.
      Calculon: No! NNNNOOOOOOOOOOOO! N! O! O! O!
    • Done again by Calculon in "That's Lobstertainment!", when Harold Zoid (Zoidberg's over-the-hill celebrity uncle) is trying to make a movie that will guarantee him an Oscar.
    • In "Mars University", Gunther the genius monkey decides to get a degree in business with his now only decent intelligence. causing Professor Farnsworth to shout a big no.
    • In "Love and Rocket", Bender dumps the crazy Planet Express ship while she, and the main cast inside her, are being chased by a large fleet from Omicron Persei 8. Cue big, shrieking no.
    • "When Aliens Attack": Fry gets one off after a ship from Omicron Persei 8 blows up his sand castle, immediately after the Omnicronians blew up many world monuments gathered in the same part of New New York.
    • And Fry again in Into the Wild Green Yonder when Bender gives him back his game (he was playing Tetris) and Bender tell him he messed up the "L" formation he was making with the blocks.
    • Fry again in "Fry and the Slurm Factory", when Leela dumps the trough of pure Slurm he'd been eating from into the sewers.
    • Fry yet again in "Time Keeps on Slippin'" when his love letter to Leela ends up being destroyed.
    • Again Fry in "Put Your Head on My Shoulder", when Fry, whose disembodied head is attached to Amy, long story, does this in slow-motion when the credit card for the date drops. Fun fact: It was not actual slow-motion, Billy West did the deep-voiced "NO!" in real-time.
    • When the giant Bender in "Anthology of Interest I" finally shuts his eye bay after his untimely death, this is the first thing that comes out of Fry's mouth.
    • Bender in "Lethal Inspection" when he learns he wasn't built with a back-up unit.
    • In "The Duh-Vinci Code" when Fry accidentally destroys the Professor's Beard of Leonardo Da Vinci, only for him to find a hidden scroll in it a second later.
      Professor: No! Noooooo! But possibly yes.
    • Played with by Bender in "The Silence of the Clamps" where, after delivering a Big "NO!" as he witnesses Calculon getting clamped into a misshapen mess in front of the Robot Mafia, describes the scene in hilariously full detail in front of the chief of police, complete with imitation of said Big "NO!". When required to testify before a grand jury, he repeats his bit-by-bit exposition of the Robot Mafia's act, only to have the Big "NO!" reproduction interrupted by the prosecutor. He asks to let him finish, then lets out a brief "o" representing the end of the Big "NO!", then says that he is, in fact, finished.
    • In fact, there's so many that somebody made a 6-minute long compilation of them.
    G – J 
  • In the Gargoyles: The Goliath Chronicles episode "Genesis Undone", Dr. Anton Sevarius yells a big "No" when he sees Thailog inject Little Anton with the virus Sevarius injected into Thailog and the other gargoyle clones.
  • In The Grim Adventures of Billy & Mandy, Billy and Grim both have a tendency to do this. Mandy gives one in "Billy & Mandy Moon the Moon" when she's turned into a milk-themed superhero, while Irwin gives one in "King Tooten Pooten" when he's sealed inside a pyramid.
  • In the Happy Tree Friends episode "Sea What I Found", Russell has a very drawn-out "Yar!" when his treasure chest gets stolen, though given the circumstances, it's obvious that he means no.
    • Played straight in "Better Off Bread" when Splendid finds out his bread burned in the oven.
  • Horrid Henry has this as a Running Gag.
  • Invader Zim just loves to scream. The Big No is his favourite.
    • From the same show we also have the most epic Big No of all time as delivered by a random guy at a park bench who's soda can is being crushed by Mars.
    • And then there's two Big No's in the episode "Zim Eats Waffles".
  • Jackie Chan Adventures:
    • Shendu yells this after ending up possessing Valmont, instead of Jackie, which was what he intended. Almost all of the Demon Sorcerers scream this when they are banished back to the Netherworld through their portals. When they banish the Earth Demon, Jade hangs a lantern on it: "Here comes my favourite part. "NOOO!""
    • Uncle is also fond of the Chinese version "Iyaaaa!" — usually just before smacking Jackie around the head for doing something stupid.
  • Jimmy Two-Shoes: Beezy lets out one as Heloise beats him in a staring eyes competition.
  • This is Johnny Bravo's reaction to finding out the Easter Bunny isn't real.
  • Jonny Quest: The Real Adventures has a really cringeworthy one with the closeup of the really primitive "Quest World" CGI face. One look may give you nightmares: https://postimg.cc/RNBcy296
  • On Johnny Test, Johnny delivers one and then the ground shakes.
    • In "Johnny's Saturday" Johnny delivers one when he finally was able to watch a fight TV show after being constantly harassed by Sissy and her girls TV show program only to find his father telling him to go to sleep right after that.
    K – L 
  • KaBlam!: Near the end of the "Little Freaks" short, after No-Face's Villainous Breakdown, the Little Freaks have this exhange before No-Face gets this trope:
    Braingel: Forget it, No-Face!
    Mike: They're moths!
    Tryla: They like the night life.
    Michael: They like to boogie.
    No-Face: OOOH NOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!!!!!!!!!!!!!
    • In the Life With Loopy short "Kung Fu Loopy", Larry lets one out after his mom suggests that he should take Loopy to "Kung-Fu Chickens On Ice" with him. His narration describes this as his "convincing argument".
  • Kim Possible:
    • In "Stop Team Go", after using hundreds of mooks to destroy the gurken jar, Dr. Drakken puts the jar under a big laser beam, and he fires it. The result was a big crater on the floor, and the jar was unharmed. Cue Big No.
    • In another episode, upon learning that her very annoying younger twins have been skipped ahead and are now in the same high-school as her, Kim lets out a big no powerful enough to knock letters of the school sign out front, while she's indoors.
    • Ron has a couple throughout the series (including a truly epic one in "A Very Possible Christmas" (Season 2, episode 16).
  • King of the Hill: This is a Running Gag. Nearly always uttered by Hank, in a semi-nonverbal, almost primal-scream manner. One of the funniest parts of the show.
    • There's a pretty epic one in "Luanne's Saga", one of the early episodes. The face he makes as he does this has become something of a Memetic Mutation.
  • Littlefoot gets one of these in The Land Before Time IX: Journey to Big Water, as he watches Mo lead the swimming sharptooth away.
  • LoliRock: Gramoor tends to do this quite frequently when his plans go awry. It ends up being his Last Words when the Princesses finally kill him.
  • A few Looney Tunes/Merrie Melodies cartoons have this:
    • The Chuck Jones Warner Brothers cartoon "Duck Amuck":
      Daffy: All right. Let's get this picture started.
      (Iris Out and THE END appears)
      Daffy: Nooooo! Nooooo!
    • The Friz Freleng cartoon "Bucaneer Bunny" has Yosemite Sam (a.k.a. Pirate Sam) say a couple of Big No's when Bugs attempts to throw a matchstick into the gunpowder stores of his pirate ship.
    • Also one near the end of the McKimson short "Sleepy Time Possum" in which Paw Possum, disguised as a dog, gets catapulted by his son.
  • Several times in The Loud House:
    • Twice by Lincoln in "Left in the Dark", coupled with Skyward Scream, when Lucy steals his spot on the couch and when he misses the show.
    • By Lincoln again in "No Guts, No Glori" after Mom and Dad leave.
    • By Lincoln a third time at the end of his fantasy in "Linc or Swim".
    • By Lincoln a fourth time in "Changing the Baby" when Mom announces they're going to Aunt Ruth's.
    • By Lola in "Undie Pressure" when Luan loses the bet.
    • By Bobby in "Cereal Offender" when Lincoln knocks over a stack of cans.
    • By Lincoln and the sisters in unison in "Snow Bored" when Lisa declares she hates snow days and prepares to melt the snow.
    • By the sisters in "Study Muffin" when Lisa deletes Hugh's picture from Lori's phone.
    • By Lincoln in "11 Louds a Leapin'" when his sled lands in Mr. Grouse's backyard. Clyde has the same reaction when he calls him.
    • By Ronnie Anne in "No Show" when Hector accidentally spoils the end of a show in front of her face.
  • In an episode of The Marvelous Misadventures of Flapjack, The Real Adventurer has this reaction to Bubbie telling him K'nuckles is not a pregnant woman.
    M 
  • Maya & Miguel:
    • In "Chrissy's Big Move", Chrissy lets one out after she hears that she and her family are moving to Hong Kong.
    • This trope was also used twice by Maggie in the episode "After School". First was in her dream when Mr. Nguyen has the kids clean up the Kindergarten classroom. Second was when she runs out of her school after an unnamed female teacher asks her if she's here for her after school.
  • Played for laughs in Metalocalypse, where Big Nos are Nathan's catchphrase, and he lets them out quite regularly. They are usually accompanied by the camera flying down Nathan's throat. One example comes when Nathan lets out a NOOOOOOOOOO! upon Nick Ibsen revealing Dethklok's parents.
    Nick Ibsen: You are all going to be reunited with your families.
    (a panel opens up, revealing the families of each of the five band members)
    Nathan Explosion: NOOOOOOOOOOOOOOOOOOOOO!!! You're going to pay for this!
    Nick Ibsen: Pay for what? Journalistic integrity?
    • The bit on flying down the throat comes from the episode "Murdering Outside the Box". When Nathan learns that someone is embezzling from the band, he suddenly goes into the Big No sequence, and from the "down the throat" bit the show goes into the opening.
    • It also happens when Nathan, after having death-growled the entire works of Shakespeare to sell as an audiobook, learns that an extremely-inebriated Pickles "like...TOTALLY forgot to hit record, man." The strain of recording the growled version of "Hamlet" having taken its toll on Nathan's larynx, the Big "NO!" was rasped rather than screamed.
    • It's hard to identify due to the polyphony of the crowd chanting, Nathan screaming, and Skwisgaar panicking, but this is what Pickles is yelling at the end of the Depths Of Humanity in The Doomstar Requiem.
  • Essentially a catchphrase for Grup in Mighty Magiswords.
  • Miraculous Ladybug:
    • "Timebreaker" has quite a few, namely Timebreaker's response to the watch being broken the second time and Ladybug's response to Alya and Cat Noir being frozen in time.
    • In "Mayura (Heroes Day - Part 2)", Carapace screams "NOOOOO!" after Rena Rouge gets akumatized into Rena Rage during a battle against Scarlet Moth and his scarlet akumatized victims.
    • Chloé lets one out near the end of the episode "Gabriel Agreste" after Bob Roth deletes her video she was gonna show to Gabriel on his phone.
    • Hawk Moth also lets out a big no in some episodes.
  • Molly of Denali: In "Busy Beavers," Trini lets one out upon realizing damming the outflow stream to save her garden didn't work.
  • Moral Orel:
    • Shapey does this at the beginning of "Charity" when Clay messes with his hair.
    • The audio of Shapey yelling is recycled in numerous episodes, including three times in "The Best Christmas Eve" and by his conterpart Block in the episodes "Numb" and "Closeface".
    • Nurse Bendy says this when her toy bear lands on her back in the episode "Alone".
  • Julie does this in the Motorcity finale when she thinks Mike was killed in the self-destruction of his car.
  • ¡Mucha Lucha! is a perfect example of this. The Flea did one when his baby sister Pulgita is about to be killed by The Masked Toilet so he went inside the toilet and Pulgita beats up The Masked Toilet; at the end of the episode, The Flea did it again when his scary story he told his baby sister when he tucked her in became true when The Masked Toilet wanted his shoes (The Flea said it three times).
    • In The Return of El Malefico, Buena Girl says this and she was too late because Rikochet read the code of masked wrestling backward, which set El Malefico free. Also, it was The Flea's fault because he tricked Rikochet into doing it while The Flea was controlled by El Malefico. At the end of the movie, El Malefico also said this because he failed to take over the world.
  • My Life as a Teenage Robot:
    • In "The Return of the Raggedy Android", the Exo-Skin screams one as Jenny is destroying it to revert to her real robot form so she can fight the space bikers.
    • Vexus at the end of "Escape From Cluster Prime", when Vega and her friends give Cluster Prime back their golden chips. Also, used in "Puppet Bride" by Mrs. Wakeman (along with a flying tackle) during a wedding between Little Acorn and Jenny.
  • My Little Pony:
    • My Little Pony 'n Friends: "The Quest for the Princess Ponies – Part 4": Lavan lets out two loud "nooooo!"s when he sees his attack reflected from the Princess Ponies and heading back towards him, right before he's blown to pieces.
    • My Little Pony: Friendship Is Magic likes this trope, with many instances about equally split between straight uses (by every Big Bad in his/her moment of defeat, except the one who is actually destroyed), characters who seem to be deliberately hamming it up, and comedic uses with no in-universe hamming.
      • In "Stare Master", Fluttershy's reaction to Applebloom suggesting the Cutie Mark Crusaders try to get coal-mining cutie marks is a very emphatic (especially for her) "NO!"
      • "A Dog and Pony Show": Spike does this twice, both times coupled with Skyward Scream: the first when Rarity is kidnapped, and another when he realizes he and the other ponies have gotten themselves lost in the Diamond Dogs' mine.
      • "The Cutie Mark Chronicles": Scootaloo does this when Fluttershy suggests singing another cutesy song.
      • In "Sweet and Elite", Rarity gives a rather mournful one when her friends decide to crash the Canterlot Garden Party.
      • "Hearts and Hooves Day": The Cutie Mark Crusaders do this at the end when Big Macintosh and Cheerilee make the Crusaders think the two of them are still under the influence of the love poison.
      • "Games Ponies Play" has several. Rainbow Dash gives one in a flashback when she learns that Cloudsdale won't be getting the Equestria Games this time. A minute later Pinkie does one that seems to come out of nowhere until she explains that she was just answering Twilight's question ("Are we going to let Cadance down?") Later, when a panicked Rainbow Dash asks, "We got the wrong pony?!", Pinkie screams, "NOOOOOOOOOOOOOOOOOOOOO!" — before correcting herself to a Big "YES!" in exactly the same tone.
      • The counterpart to this episode, “Just For Sidekicks” has Spike doing it twice, both times because he ate all the gems he was going to put in the cake he was making.
      • "Party Pooped": Pinkie does a rather prolonged one right when she gets to Yakyakistan and the snow under her sled breaks, sending her sliding all the way back to Ponyville.
      • "The Beginning of the End – Part 2": King Sombra lets out a big no when he's destroyed by the Mane Six's friendship magic, without any Elements.
    N – P 
  • Nature Cat:
    • The titular character himself does this various times throughout the series, particularly in the episode "Slime Time" when he finds his yarn ball gone and yells out, "NOOOOO!", complete with a Skyward Scream.
    • Ditto for Hal, especially in the special, "Ocean Commotion" after Daisy told him that there is no way the ocean's current cannot stop moving as Hal's chew-toy Mr. Chewinsky floats away. And again near the beginning of "Moth Frolic-Fest" after he discovers that Nature Cat, Squeeks, and Daisy frolicked with butterflies for the whole day without him, much to his dismay.
  • Happens in an episode of Nightmare Ned by the Tooth Fairy when Ned wins a game of checkers against a giant tooth for ownership of his teeth.
  • LEGO Ninjago: Masters of Spinjitzu: Said by Zane when he discovers that he is a robot.
  • In the Oh Yeah! Cartoons short "Super Santa: Vegetation", the villain Dr. Carmine Miranda yells "No" after his mutant vegetable minions are turned back into normal vegetables.
  • The Penguins of Madagascar are in love with this trope. Most come from Skipper.
  • Phineas and Ferb: Yes, Yes it does have it too, for example:
    • Parodied in "Lawn Gnome Beach Party of Terror" the radio DJ at first lets out what appears to be a big no... but he's actually screaming "Gnomes"!
    • In "Backyard Aquarium", Phineas shouts one when Goldie is swallowed by an orca, only to come out of its blowhole unharmed.
    • In "Gaming the System", Candace does this in response to her brothers' Disney Death. A few minutes later, Phineas yells one when the game's final boss goes after Isabella.
    • In "The Chronicles of Meap", Mitch apparently lets off a Big No when Meap confiscates his Universal Mustache Translator, but it comes out as a Big Meap.
    • Candace lets out another Big No in "Invasion of the Ferb Snatchers", on realizing she's missed yet another chance to bust her brothers. Earlier, two of them are done by Amanda in the movie when she sees the alien who took over Dr. Abernathy's body.
    • Doofenshmirtz screams one in "The Great Indoors" when his Soap Within a Show gets preempted.
    • And Baljeet gives a rare stupidity-induced one in "When Worlds Collide" when trying to explain to Buford what it means when worlds collide, and Buford says "It's like when you've got two sets of keys, and you leave one in the car."
  • In the first episode of The Hub's Pound Puppies, adoptee-of-the-week Yipper lets out a cross between this and a howl of despair when it looks like he's missed his chance to get adopted by his "perfect person".
  • The Powerpuff Girls:
    • "Parent teacher conference?! NOOOOOOOOOOOOOOOOO!!!"
    • From "Mo'Linguish":
      Judge: You, Mojo Jojo, are hereby sentenced to teaching a knowledge extension course at Townsville Community College!
      Mojo Jojo: NOOOOOOOOOOOOOOOOOOOOO! By which I mean I cannot do it.
    • "Gettin' Twiggy With It": The girls scream "NOOOO!" in unison when Mitch flushes Twiggy down the toilet.
    • "Fallen Arches": Captain Righteous and Lefty confront the Ministry of Pain. The Captain lets out a big no as he runs towards Lefty who just hurt his hip. However, he hasn't reached his sidekick yet so he shouts it again.
    • "Get Back Jojo": Mojo Jojo lets out a frustrated "NOOOOOO!" at the end of the episode when the Narrator points out that his trip through time accidentally inspired the creation of the Powerpuff Girls. Followed by the Narrator doing the inversion.
    • The comic book story "Steal A Meal" (Cartoon Network Block Party #26) has the girls doing a tearful Big "NO!" upon learning that after all the breakfast cereal in town had been stolen the only thing left was oatmeal.
    • "Mime For A Change": Bubbles performs a great Big "NO!", coupled with Skyward Scream, when she realizes her crayons won't restore her sisters.
    • For the good example in "Knock it Off": Dick Hardly does this when his own creations start killing him.
    R 
  • Ready Jet Go!: Jet's cousin Zerk screams "NOOOOOOOOO!" at the end of "Whole Lotta Shakin'" after Jet throws his smartwatch away, which means Zerk can no longer bother Jet.
  • ReBoot: Done straight on a fairly regular basis. Bob, being shot into the Web at the end of season two, Enzo, after learning about Bob's fate, Dot in "Game Over" when it appeared that Enzo had been nullified, and said twice by Megabyte in two episodes toward the end. This YTMND does a rather good job of pointing out just how often this trope is played.
    • Lampshaded in the musical recap at the end of the third season:
      Singer: But Megabyte betrayed Bob and
      He threw him deep inside the pit.
      The pit was closed,
      and Bob was hosed,
      and all that he could say was-
      Actor Bob: Noooo!
  • Recess uses this a number of times, a few being "No...no...NOOOOOOO!!!!!":
    • "The Break In": Towards the end, by T.J. when he just broke himself out of detention and onto the playground, just as recess ended for the day.
    • "The New Kid": By Gus when his name is taken away and must be referred to as "New Kid"
    • "The Experiment": By T.J. and Spinelli when they find out they have to kiss each other to see what the effects were on the opposite sex.
    • "The Legend of the Big Kid": By Vince when he and the gang are looking for T.J.
    • "Mama's Girl": By Spinelli when no one will stop taunting her after she accidentally called Miss Grotke "mama".
    • Mikey, in various episodes, especially twice in the movie: the first time where TJ found out the full extent of Dr. Benedict's plan, and the second before the final battle.
    • "First Name Ashley": By Spinelli when Randall reveals her first name to the students.
  • The Death Kwon Do instructor in Regular Show gets three rather nice ones. First when he discovers Rigby stole the instructions to learn the Death Punch of Death, only to be interrupted by a student explaining the toilet was 'Death Kwon clogged', leading to another Big "NO!". Then finally, when he discovers that Mordecai stole the Death Jump and Death Dump instructions, he lets out another Big "NO!".
    • Benson seems to be fond of this trope. He had done this in a flashback in "Stick Hockey", after being fired in his thoughts in "Think Positive", "Prankless" when he sees Mordecai and Rigby get shot with tomatoes, when Mordecai loses on purpose in "Dodge This", a flashback in "White Elephant Gift Exchange", and "Benson's Suit" when his suit was stained.
    • Skips gets one in "Bachelor Party! Zingo!!" when one of the Zingos mocks him by saying that he failed Quips like he failed to prevent the death of his love interest in "Skips' Story".
    • Mordecai and Rigby do this in unison in the episode "Muscle Woman" after realizing that they're tracking footprints in the sand after cleaning the park rock garden while doing all of Muscle Man's work.
  • Rick and Morty
    • Rick lets out a couple after a group of scamming aliens fully reveal the nature of their scheme to him.
    • He lets out another in the season 2 finale when Birdperson, his best friend, is killed.
    • Yet another in the season 3 premiere during Rick's faked flashback to losing Beth and his wife, then the Federation agent who was sent into his brain gives a genuine one when Rick steals his body.
  • The Ren & Stimpy Show: In "Sven Hoek" Ren lets out a Big "NO!" after he sees that Sven and Stimpy ruined his collection of rare incurable diseases.
  • Rocket Power:
    • Used as the last lines of the Halloween Episode, "The Night Before" by the rocket gang when Ray announces to Violet that they'll only get a piece of candy once per visit to trick or treat, followed by a howling wolf.
    • Conroy lets this one out at the beginning of "Say Hello to Cement Head" when he finds the damage Twister did to his brand-new vert ramp mere moments after the gang flees the construction site, kicking off the plot of the episode.
  • Rocko's Modern Life:
    • In the episode "I See London, I See France", Rocko and Heffer are on a bus tour of Paris with a bus driver who is determined to keep his passengers on the bus at all costs, so much so that he chases them around the city after they escape. By the time the two board a plane home, the bus driver delivers an epic BIG NO as the two fly off into the sunset.
    • Done again near the end of the episode "Cruisin" when the boat rides back to the dock and the ship's captain yells it out as he runs towards the screen and eats the camera.
  • In a Robot Chicken sketch involving Archie and his friends dealing with deaths plaguing everyone, Moose lets out a Big Duh when Ms. Grundy is crushed by a car.
    • In the Star Wars special (third one), Vader slips on something, and falls. His hand gets stuck in the toilet, and the toilet flushes. Cue the: "NOOOOOOOOOOOO!"
    • In the first season finale parodying You Can't Do That on Television, the Bloopers host is being held prisoner and lets one out when he's told that the only movie his torturers have is Christmas with the Kranks.
    • In the "Heimlich Begins" sketch, the titular character lets out a Big "NEIN!" when his father chokes to death on candy after watching a movie.
    S 
  • Sabrina: The Animated Series: Gem lets one out in the intro after getting her clothes magically changed by Sabrina's broom zooming past her.
  • Samurai Jack episode 23 "Jack versus Demongo, the Soul Collector". Demongo uses one after Jack defeats him.
    • And in Season 5, Jack himself lets out one when he believed the blue-furred children from the factory are dead.
  • Sev Trek: Pus in Boots (the Australian CGI spoof of Star Trek: The Next Generation). Captain Pinchhard utters one of these upon hearing that Measly Cruncher is going to save the Enterforaprize for the 47th time. He stops this from happening by vaporizing Measly.
  • The Sheep in the Big City episode "My, How Ewe Have Changed" has General Specific capturing Sheep when he returns to the farm for a barbecue reunion with his old friends. Farmer John notices this while in a scuffle with some of Specific's soldiers. He then yells "No" while throwing the soldiers off of him.
  • In the first episode of SheZow, Guy has this reaction once he learns that his aunt's magic ring won't come off his finger and that he would be forced to carry the duty of SheZow for the rest of his natural life. This inadvertently triggers his sonic scream power and he destroys the mirror guarding the entrance to his aunt's secret lair.
  • The Simpsons:
    • In the episode "Mayored to the Mob", bodyguard-in-training Homer practices his slow-motion dive. Making it funnier, the instructor is voiced by Mark Hamill (who starred As Himself in the same episode), who should know his Nooooos...
      (Homer gives a running dive with an unenthusiastic Big No)
      Instructor: Well, your dive wasn't bad, but I just didn't believe your 'NOOOOOO'! You gotta sell it! Remember, your 'NOOOOOO' is what gets you your next job! Now drop and give me twenty.
      Homer: NOOOOOOOOOOOO!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!
      Instructor: Better!
    • Used straight in episode "The City of New York vs. Homer Simpson", when Barney discovers he holds the black pickled egg that means he's today's designated driver.
      Barney: (after noticing the others have normal eggs and slowly seeing he got the black one) NOOOOOO! (crushes the egg in his hand and holds his head)
      Homer: (coming up to him) You got the black one.
    • Also used straight by Bart in "Black Widower".
    • Seymour Skinner releases 4 of them in "Bart's Comet" firstly, when he sees the big skinner balloon for the first time. Secondly, when he discovers that Bart has managed to discover a comet, something Skinner has dreamed of for years, when he briefly left Bart in charge of his telescope whilst trying to capture a less-than-complimentary hot-air-balloon depicting Skinner with a giant rear that Bart earlier released. Then, he realises that in his earlier grief he's released the hot-air-balloon, letting it float free to humiliate him further. Finally, a paperboy drops the morning paper in front of him, with a headline reading "PREZ SEZ: SCHOOL IS FOR LOSERS":
    • Skinner pulls a similar one in season 4's "Duffless".
    • In season 4's "The Front", when retaking his high school Remedial Science A1 class, Homer lets one out when Principal Dondelinger burns a donut in front of the whole class to show how many calories it has.
    • Done yet again in "Homer and Lisa Exchange Cross Words". Homer makes Lisa so angry that she tells him that she's going to use Marge's maiden name (Bouvier) instead of Simpson. Homer drops to his knees and screams "NOOOOOOOOOOO!" Then another girl walks by and asks if he wants to buy some Band Candy. Homer's reply: "YEEEEEEEEEEEES!"
    • And then there's "Home Sweet Homediddly-Dum-Doodily":
      Ned: Do you reject Satan, and all his empty promises?
      Homer: NOOOOOOOOOOOOOOO!!!!!!! (skin burns on contact with holy water)
    • "Guess Who's Coming to Criticize Dinner?":
      Lisa: Dad, no! [The poisoned eclair] is going to kill you!
      Homer: Eh, I've had a good run. (prepares to take a bite)
      Lisa: No! (thinks) It's low-fat!
      Homer: NNNNNNNNNNNNNNNOOOOOOOOOOOOOOOOOOO! (throws eclair)
    • And parodied yet again in an episode where Grandpa is a Sprawl-Mart greeter, and gets knocked onto an out-of-control shopping buggy.
      Grandpa: NOOOOOOOOOOOOOOOOO— (cut to show that he's headed for a stack of lawn gnomes) —OOOOOOMES!
    • In "Homer's Barbershop Quartet", Chief Wiggum lets out one that gets twisted into a wolf-type howl at the moon.
    • In the beginning of "The Bart Wants What It Wants", Marge threw the Olympic Torch to the Olympic Administrators and the helicopter crashed.
      Administrator 1: Are you okay?
      Administrator 2: Yeah, I'm ok...(looks at extinguished Olympic Torch) NOOOOOOOOO!!!!!!!!!!
    • In "The Simpsons 138th Episode Spectacular", alternate clips are shown including another ending as to who shot Mr. Burns. When he fingers Smithers as the culprit, Smithers gives a big no then adds, "Wait a minute. Yes."
    • Played for Laughs in "Marge Gets a Job". Smithers is finished cleaning the unirals until...
      (Homer bursts in)
      Homer: Oh, man! I really gotta— (unzips fly)
      Smithers: NOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!!
    • Apu does this when Taking the Bullet for James Woods in "Homer and Apu".
    • In "Sideshow Bob Roberts", Bart learns that his nemesis Sideshow Bob is to be set free from prison leading to Big No. However, he learns this in the middle of class while secretly listening to a radio, prompting a rather bemused response:
      Ms. Krapabbel: ... Well. Despite Bart's objections, the people of South Africa can now vote in free democratic elections.
    • In "Scuse Me While I Miss the Sky", Lisa, who doesn't want the town's lights on at night, lets one out as Mayor Quimby flips the switch turning on every light in town.
    • In "Homer the Vigilante", an angry mob shows up at the Simpsons' door after Homer fails to prevent a notorious cat burglar from stealing a cubic zirconium from the museum, leading to Jimbo losing respect for him.
      Jimbo: You let me down, man! Now I don't believe in nothin' no more! I'm going to law school!
      Homer: NOOOOOOOO!
    • In "Lisa's Date with Density", after she helped Nelson escape from the cops, he looks to see Skinner's reaction as he's cleaning his house after a rancid food attack:
      Nelson: Wait until he sees what I left in his birdbath.
      Skinner: NOOOOOOO!
    • "Treehouse of Horror IX":
      • In "Hell Toupee", Snake is executed and Homer gets his hair for a transplant. However, Snake's hair starts to possess him and uses him to hunt down the people who witnessed his arrest being executed (Apu, Moe, and Bart). He starts with Apu, whom he attacks at the Kwik-E-Mart, and we hear Apu screaming "NOOOOOOO!" when we cut away.
      • In "Terror in Tiny Town" has Scratchy, after being transported from the TV, falling for Snowball II. Marge thinks it's sweet, then she tells him that he'll have to be neutered.
        Scratchy: (while holding his privates) NOOOOOOOOOOO!!!
    • In "Catch 'Em If You Can", when Bart sabotages Homer and Marge's flight to Atlantic City:
      Bart: So, Mom and Dad are going to Atlantic City, but their luggage isn't. And Homer's getting the low-fat meal.
      (cuts to the outside of the plane that Homer and Marge are on)
      Homer: NOOOOOOO!
    • In the Itchy and Scratchy cartoon "Bleeder of the Pack", featured in the episode "C.E.D'oh", Scratchy lets one out when he finds out he's on the same airplane as the Big Bopper, Richie Valens, and Buddy Holly.
    • In "Treehouse of Horror XXIV", during the segment "Oh, the Places You'll D'oh!", Apu lets out a Big No when the Fat in the Hat (Homer) condemns him to a fate worse than death - having to spend time with his wife and eight children.
    • In "Fatzcarraldo", this is Homer's reaction to being told that Krusty Burger has replaced their ketchup with beet aioli as part of their new, healthier menu.
  • South Park:
    • In "Cat Orgy", when Skyler discovers his trashed guitar.
    • "Jewbilie" does it four times in a row: First Kyle when Kenny makes a Heroic Sacrifice to stop Haman, then Haman when Moses escapes the conch shell, then anti-semetic Rabbi Garth screams it twice when he realizes his plans have been foiled.
    • "Probably": God gives Saddam Hussein an even worse punishment than sending him to Hell: sending him to Heaven.
      Mormon: We were just about to do a play about how much stealing hurts you deep inside. Come join us. (the Mormons lift Saddam over their heads and carry him off)
      Saddam: No! NOOOOOO!
    • "Something You Can Do With Your Finger" parodies Captain Jean-Luc Picard's scream from Star Trek: First Contact, when Randy prevents Stan from joining a boy band with his friends. Incidentally, they use the actual sound clip of Picard's breakdown from the movie for that scene.
      Stan: Why can't I be in a boy band?
      Randy: (firmly) Because I Said So.
      Stan: Cartman said we're going to perform at the mall at three o'clock! My friends are gonna be pissed off at me!
      Randy: (still pretty controlled) Let them be pissed off.
      Stan: Dad, I don't understand. Just let me do this one thing and then I'll...
      Randy: (inexplicably losing it) Noooo! NOOOOOOO! (smashes a glass dresser with his head)
    • In "The Last of the Meheecans", Randy does it again when he's confronted with what happens when Mexican immigrants performing menial labor all decide to move back to their birth country in a fit of nostalgia, using the same Darth Vader soundclip from Revenge of the Sith.
    • In "Dances with Smurfs," Ike delivers one upon hearing that Wendy wants to kill Smurfs.
    • In "Marjorine" Linda Stotch lets out several blood-curling screams of "NO" when she thinks her son Butters has committed suicide by jumping off a building. She does it again at his funeral while throwing herself on his casket.
  • Parodied in the Space Ghost Coast to Coast episode "Boo Boo Kitty", when Space Ghost's pet cat, actually a large balloon, slowly drifts towards a large safety pin. He screams NOOOOOOOOOOOOOO!, runs out of breath, inhales, and starts again. After the balloon deflates, he promptly ignores the others when they suggest he could have easily saved Boo Boo Kitty from its untimely death.
  • Space Goofs:
    • Etno lets one out in the episode "Once Upon a Time" when Candy presses the button that launches his spaceship.
    • Etno again in the episode "One Minor Technicality" when a boy gets his spaceship box.
  • In The Spectacular Spider-Man, the Sandman gets one of these after seeing the results of his Transformation Trauma into a living sand being.
  • Spider-Man: The Animated Series:
  • SpongeBob SquarePants: Used a lot in this show, for example...:
    • Plankton lets one out at the end of "Plankton!" after he ends up stuck in his computer wife Karen.
    • SpongeBob does this in "Pickles" when he finds out he forgot the pickles.
    • "Nature Pants":
      SpongeBob Patrick, I'm not coming home.
      Patrick: I miss you! Sandy misses you! Even Squidward misses you! (Squidward is throwing himself a party at home)
      SpongeBob: I'm happy here. This is my home now. (Sandy drags Patrick away)
      Patrick: Nooooo!! SpongeBob!!
    • Squidward does one in "Opposite Day" when he sees the realtor's car.
    • Mr. Krabs lets one out in "F.U.N", when Plankton steals the formula.
    • Employee of the Month: "NOOOO! How could I have let the quality of my work slip so much?"
    • "Sleepy Time":
      SpongeBob: Mrs. Puff! Look! I've finally got my driver's license!
      Mrs. Puff: (takes his license and tears it up) Not even in your dreams, Mr. SquarePants!
      SpongeBob: Noooo...!!! (flies out of his dream)
    • "Valentine's Day":
      Sandy: We're going down, SpongeBob! Switch to plan B!
      SpongeBob: No! No B, Sandy! NO B!!! NOOOOO!!!
    • In "Something Smells":
      Patrick: Maybe it's just because you're ugly.
      SpongeBob: Ugly?! (sticks finger in mouth, slides it across his eyebrows) You gotta be kiddin' meh.
      Patrick: Let us try the reflection test. (holds up a mirror in front of SpongeBob)
      SpongeBob: (to reflection) Hi! (SpongeBob's reflection grabs a hammer and breaks itself after smelling SpongeBob's breath)
      Patrick: Uuuuuuglyyyyyyy....
      SpongeBob: OHHHHHHHHHHHHHH NOOOOOOOOOOOOOOOO!!!!!!!
    • Also used in "Bubble Buddy", in dramatic slow-motion and with added vowel screwing around with.
    • And used twice by the live-action Artist in "Frankendoodle", first when he accidentally drops his pencil into the ocean at the beginning of the episode, then when he gets it back only to break it at the end of the episode.
    • SpongeBob during the Overcrank in "As Seen on TV" when he believes his "career" is over.
    • SpongeBob and Patrick in unison in "The Camping Episode" just before Squidward suffers another sea bear attack (from limping).
    • Two of them in "New Digs"; the first is by SpongeBob when he is late to work, and the second is by Squidward when Mr. Krabs evicts SpongeBob from living at the Krusty Krab, because with SpongeBob living there Squidward was finally getting some peace at home.
    • In "Fungus Among Us", SpongeBob screams one when the angry customers are close to destroying his protective bubble which contains all the ick he consumed.
    • In the first movie, Plankton lets one loose when SpongeBob began zapping the mind-controlling helmets off the Bikini Bottom residents using The Power of Rock. Mindy also shouts three of them, coupled with Plankton's Big "YES!", when King Neptune is about to execute Mr. Krabs.
    • In "Squidtastic Voyage", SpongeBob, Squidward and Sandy scream one in unison when Patrick presses the submarine's "grow" button while they are still in Squidward.
    • SpongeBob in "Yours, Mine and Mine" when Patrick eats the Patty Pal toy, which immediately summons Mr. Krabs over to resolve the feud.
    • Gary does this in "Treats!"...with a big long meow as a substitute causing the whole screen to shake.
    • "Glove World R.I.P":
      Glove World Owner: Well, enjoy that hat. Now if you'll folks will excuse me, I have a rickety old theme park to close down.
      SpongeBob: Then the rumors are true, you are gonna close Glove World.
      Glove World Owner: Yep.
      SpongeBob & Patrick: NOOOOOOOO!
      [The Glove World Owner covers his ears]
      Glove World Owner: I understand your concern, but really...
      SpongeBob & Patrick: NOOOOO!
      [The Glove World Owner covers his ears again and cut to interior of Glove World where their scream can be heard]
    • From "I'm With Stupid":
      SpongeBob: Look! 2+2-4!
      Marty: Wow, son! You taught him math, too!
      SpongeBob: NOOOOOOOOO!!!
      Marty: And you taught him to sing!
    • At the end of "Wishing You Well", Mr. Krabs, skeptical of the wishing well that he built actually being magic even after Squidward, Sandy, Mrs. Puff, and Plankton all had their wishes come true, jokingly wishes that he was steamed and served with a side of melted butter. SpongeBob gives this trope right before Mr. Krabs' wish comes true, then Mr. Krabs gives one himself as the episode ends with him about to be eaten.
    • In SpongeBob's dream at the beginning of "Blackened Sponge", he plays rock, paper, scissors with Jack M. Crazyfish and sees him cheat by shifting from rock to scissors after SpongeBob draws paper. When the real Crazyfish does the same trick at the end of the episode, SpongeBob doesn't notice due to having two black eyes and this is his reaction.
    • SpongeBob screams one in "Best Day Ever" when Squidward's concert ends right when he sits down.
    • Patchy the Pirate lets out a big no at the beginning of the special "Atlantis SquarePantis" after he realizes that Encino is missing as he ends up at the middle of nowhere.
    • Plankton in "The Inside Job" when he is about to end up in SpongeBob's head instead of Mr. Krabs'.
    • SpongeBob screams another Big No at the very end of "The Abrasive Side" when Grandma SquarePants reveals she has the abrasive side now.
    • "Single Cell Anniversary" couples this with a Big "YES!":
      Karen: (in tears from Plankton's song) Krabby patty secret formula loading completed...!!
      Plankton: YES!
      SpongeBob: NO!
      (Karen shorts out from the tears and shuts down)
      Plankton: NO!
      SpongeBob: YES!
    • Twice in "SpongeBob SquarePants vs. The Big One"; the first is done by Patrick when the Big One eats his surfboard, and the second is delivered by SpongeBob when he sees JKL choose to sacrifice himself to the Big One to save Mr. Krabs' cash register.
    • Squidward screams one in "Jolly Lodgers" when he sees SpongeBob and Patrick in the hallway, followed by another when he ends up at Jelly-Con.
  • Star Trek: Lower Decks:
    • In "Moist Vessel", Ensign Tendi screams "NOOOOOOOOOO!!!" when Lieutenant O'Connor is dying from being crushed by a large rock.
    • In the flashback of "Cupid's Errant Arrow", Ensign Mariner shouts "NOOO!!!" when her friend Angie is attacked by a Harvongian shape-changer.
  • Being a Star Wars animated series, Star Wars: The Clone Wars has instances of the use of the Big "NO!" trope:
    • In Brain Invaders, Barriss Offee screams "NO!!!" as she gets infected and possessed by a Geonosian Brain Worm offscreen.
    • Anakin pulls off this trope twice in the Mortis Trilogy. First, when the Son of Mortis kills Ahsoka, and second when when the Son shows him what he will become in Revenge of the Sith.
    • ARC Trooper CT 27-5555 "Fives" screams one when Hardcase tells him that he's going to sacrifice himself to destroy a Separatist Supply Ship after the shields to the reactor core were raised.
    • Obi-Wan does it a couple of times: first, a quick one when Zygerrian Prison Warden Keeper Arguss activates the control panels to the slaves' quarters to drop them all into the pit of doom below. The second time is right before Savage Opress gives Adi Galia the finishing stab with his lightsaber after impaling her with his horns.
  • Star Wars Rebels: At least twice with Ezra, when he's saving Zeb from Agent Kallus and when the Grand Inquisitor threatens to kill his friends. Kanan also invokes this three times in concern for Ezra, in "Rise of the Old Masters", "Gathering Forces", and "Fire Across the Galaxy".
  • Steven Universe:
    • The premiere episode "Gem Glow" opens with the title character letting out one of these when he finds his favorite snack, Cookie Cats, has been discontinued.
    • Amethyst gets in on the act in "Monster Buddies":
      "Noooooooo! Mi torta!"
    • In "Steven Floats", Steven lets out a "Noooo!" after having an Imagine Spot about missing the first batch of fresh-made donuts at the Big Donut.
    • In "Reunited", Greg's offer to use "the old Universe charm" on Blue Diamond is met with a resounding "NO!" from the other characters, perhaps recalling how the last time Greg tried to charm a Homeworld Gem, it got him abducted and trapped in a People Zoo.
  • In Street Fighter, Guile gives one when Cammy defected to Shadaloo and gives Bison a big kiss. Note the Soundtrack Dissonance.
    • Also used in some episodes, such as the one episode when Vega yells this after Blanka attacked Vega's face and after Vega looks at his reflection in the water.
    • In another episode, Fei Long yells this twice.
    • During "The Strongest Woman in the World," a young Chun Li yells this in a flashback as she witnesses M. Bison killing Chun Li's father.
    • The final one is delivered by Cammy in the final episode when she realizes that everything that M. Bison said was lies.
  • The Super Mario Bros. Super Show!: Koopa has one in "Elvin Lives" when he realizes he's driving his car towards a cliff.
    • This exchange from "Princess, I Shrunk the Marios":
    Koopa: Know what I'm gonna do with you buttinskis?
    Mario: Say you're sorry and let us go?
    Koopa: NO!! I'm gonna lock you up for two hundred years!
    • Koopa lets out yet another in the Super Mario World episode "Rock TV" when he's about to get crushed by his giant television.
  • Often used by SWAT Kats villains such as Dark Kat and The Pastmaster when the heroes defeat them.
    T 
  • Teacher's Pet:
    • The first episode "Muttamorphosis" has Leonard yell "No" after finding out his classmate Scott Leadready II is actually his dog Spot.
    • In the second Christmas Episode "The Blight Before Christmas", Leonard's mom screams a Big No in response to the delivery truck that was her last chance at getting her son the Mach III Game Command system he wanted being stripped of its contents by a crowd of people that beat her to the truck.
  • In one episode of the 2003 Teenage Mutant Ninja Turtles series, Baxter Stockman lets out a big "WHY?!" after Bishop brought him back from the brink of death (as a result of the events in "Insane in the Membrane"), which robbed him of finally resting in peace after everything he lived through in the series. It... comes across a bit narm-y.
  • In the episode "Requiem" of the 2012 Teenage Mutant Ninja Turtles series, Leonardo screams a Big No when the mortally wounded Splinter falls to the ground after being thrown from the rooftops by Super Shredder.
  • Used in the Teen Titans (2003) episode "Birthmark" by Raven when Slade shows her a vision of a destroyed world that she's going to create. Also used by Raven in the same episode when she refuses for a final time the party the others have planned for her birthday and destroys everything with her powers.
  • 3-2-1 Penguins!
    • In Comedy of Errors, Michelle does this when Jason sings the song she sings when she's scared at the Comet Lounge.
    • In Wise Guys, Jason does this twice, and both times coupled with a Skyward Scream: the first when he discovers that the batteries are not included with the time machine, and another when he is told that he and his sister are stuck in the future forever.
    • In Oh, Mercy!, Zidgel, Jason and the Gator King yell this in slo-mo when the emerald falls out of its case and smashes into many pieces.
  • In Thunder Cats 2011 Lion-O performs one that overlaps with a Slow "NO!" and a Futile Hand Reach upon seeing his father King Claudus stabbed In the Back.
  • A Tom and Jerry short revolved around a duckling imprinting on Tom and Tom taking advantage of this to attempt to cook the duckling. Near the end, the duckling realizes that Tom intends to eat him and prepares to take a long walk off a soup spoon into a bubbling pot, "If my nice mommy wants a duck dinner..." Tom utters a Big No as he catches the duckling. A "No!" made all the more impressive by the fact that Tom and Jerry usually don't have speaking voices...
  • Total Drama:
    • Owen says a big no when a box of chocolates gets knocked into the water.
    • Lindsay lets out one when she's told they won't get to go shopping in New York.
    • Also, Alejandro in the finale of the 3rd season gives out a Big No after he is put in a robot suit because he was burned by lava and trampled by most of the contestants. Al lets it out when he asks Chris if the prize money is safe, only to find out it was burned in the volcano. It's hilarious because it parodies the cheesiest moments in Star Wars history.
    • In Alejandro's ending in World Tour, when Heather realizes that she threw the wrong sacrifice into the volcano, resulting in Alejandro winning by a technicality, she lets out a Big No.
  • Uttered by Kabuto at the end of Tokyo Mater as a result of him losing to Mater in a drift race and is about to have all of his modifications pulled off his body by all of the other cars in Tokyo, who then laugh at him when Kabuto is finally naked.
  • Transformers:
    • Optimus Primal gets quite a few in Beast Wars, most notably in the cliffhanger of the first season finale, and when the Axalon is destroyed in season 3.
      • Inverted with Megatron, as he usually says "Yesssssssssssssss."
      • Last episode of Beast Machines. Megatron's final word? "NOOOOOOOOOOOOO!"
    • And in Armada, Optimus Prime gets one of these when Smokescreen dies.
      • Megatron later gets one when Starscream of all people is killed by Unicron.
    • Parodied in the "Bee in the City" script reading of Transformers: Animated, with David Kaye using his Beast Wars Megatron voice (to enthusiastic approval from the crowd). Upon his defeat, he vows to return, ending with a big "YEEESSS!"
    • In Animated, the Autobots discuss having to leave, and Bulkhead wonders how Sari (human sidekick) will take it. Bumblebee says she's a big girl and can handle it. Cut to Sari screaming NOOOOOOOOOOOOOOOOOOOOOOOOOOOO!! at the top of her lungs.
    • Not to mention the episode "Nature Calling" when Prowl smashes Bumblebee's only battery, rendering him unable to see movies in the middle of nature. His "NOOOO!" was truly epic.
    • Transformers: Prime:
      • Knock Out does one when Starscream punishes him (by scratching his precious finish) in "Speed Metal".
      • Miko does one when Bulkhead almost gets beheaded in "I.M.I.".
      • Arcee yells out a "No!" when a replication of Unicron was about to kill Optimus.
      • Megatron gets a magnificent one during "Orion Pax Part 3" when Jack restores Optimus's memories and the Autobots escape.
      • In the Grand Finale, Optimus' reaction to Bumblebee's apparent death is half this, half an inarticulate scream of rage.
    • Transformers: Rescue Bots:
      • The episode "The Riders of Midwinter" has Dani scream "No" after hearing that the canister containing everyone's Midwinter Morning presents has sunk to the bottom of the ocean.
      • At the end of "Cody's 11", Madeline Pynch yells a Big No after learning that her getaway car is actually Quickshadow and that Quickshadow is going to deliver her to jail.
    • Transformers: Robots in Disguise (2015): "Defrosted" has Fixit scream "No" after the handheld video game he was engrossed in gets shattered by the Bee Team returning from their latest mission.
  • Little Dog in the 2 Stupid Dogs episode "Cat!" after Big Dog goes back to sleep without scaring the cat.
    • The episode "Door Jam" ends with Little Dog repeatedly screaming "No" when he and Big Dog are trapped inside the Y-Mart while the can they spent the episode trying to get by entering the Y-Mart is on the other side of the door. It zooms to outside the Y-Mart's parking lot, the whole city, and then the world as he keeps screaming "No!"
    U – Z 
  • Yelled by Unikitty in the Unikitty! episode "Sparkle Matter Matters".
    Dr. Fox: This kid is eating all alone.
    Unikitty: Oh no! That's really sad! Where's all of his friends?
    Dr. Fox: They didn't show up to his birthday party.
    Unikitty (turns blue): Oh no! It's his birthday and no one's there?
    Dr. Fox: And the kid is actually... Puppycorn!
    Unikitty (blue): Little bro! NOOOOO!!!!
  • In VeggieTales, Larry the Cucumber did this when Archibald inserted "His Cheeseburger" in the jukebox. "NOOOOOOOOOOOOOOO..."
    • By Haman (Mr. Lunt) in Esther... The Girl Who Became Queen when he finds out he's getting banished to the Island of Perpetual Tickling.
    • Two by Don Quixote (Archibald Asparagus) in Asparagus of La Mancha, one when he wakes up from his first bad dream, one when Poncho (Mr. Lunt) weans him off the salsa, due to finding out that Don's addiction to his super-spicy salsa is causing the bad dreams.
    • By The Bad Apple in Larry-Boy and the Bad Apple, when her webs dissolve by using water.
    • By Archibald Asparagus in the silly song The Biscuit of Zazzamarandabo, when everyone needs to take a potty break.
    • Also by Larry in the silly song Sippy Cup combined with Eat the Camera when Jimmy Gourd explains why Larry needs a sippy cup.
  • In the last episode of the third season of The Venture Bros., Henchman 21 indulges in a Big No when the Monarchmobile explodes with Henchman 24 trapped inside, but is interrupted when 24's severed head hits him in the chest.
  • Played straight in Season 4 of Voltron: Legendary Defender when Pidge's search for her brother leads her to a massive memorial on the site of a past battle and a grave with his name on it. He turns out to be alive, but her breakdown and agonized scream at his "grave" still left many a viewer in tears.
  • Wakfu:
    • Adamaï lets out a Big No (with Skyward Scream) at the end of episode 16, after Grougaloragran teleports the heroes away to fight Nox alone.
    • Nox, as part of his Villainous Breakdown in the season 1 finale, when he realizes that his attempted Time Travel only brought him back twenty frigging minutes in the past.
    • Qilby briefly unleashes a frantic, desperate one when begging Yugo to come back and not leave him trapped in the White Dimension again.
  • Wander over Yonder does this a lot:
    • By Sylvia in "The Pet", coupled with Skyward Scream, when she thinks Wander has been killed by the alien when really, what she found was the remains of a teddy bear he gave it.
    • At the very end of "The Giftening", Hater screams this after reading Wander's thank you note and the episode ends. It is suddenly followed by a Big "YES!" in its P.O.V. Sequel "The Gift", which continues slightly past where the episode ended.
    • In "The Bot", Dominator screams it when Beep-Boop deletes all the information on Volcanium X after remembering all the wonderful times spent with Wander.
  • Used in The Weekenders, during the episode "Cry", when the gang is making fun of Tiro shedding a tear over Romeo and Juliet while hanging out at the pizza place:
    Carver: How can anyone get upset over something as dumb as that?
    Lor: Pizza's jammed.
    Carver: NOOOOO!!!
  • In the beginning of the Whatever Happened to... Robot Jones? episode "Work", Robot hollers "No" when he runs out of quarters and gets a Game Over on the arcade game he's playing.
  • This has happened more than once in Winx Club.
    • Lord Darkar lets out a number of Big Nos as the Winx use a Charmix convergance to finish him off for good.
    • Miele screams, "FLORA, NOOOO!!!!!" as Flora sends her back up to the surface prior to nearly drowning in the Water Stairway. Earlier, she also screams this when jumping in front of Flora to take the bullet from an attack by the Trix.
    • Bloom screams this after Tecna is sucked down the Omega Portal.
    • Aisha screams this when Ogron wastes the Black Gift and Nabu dies for good.
    • Icy screams one when Selena locks the Legendarium for good, with the Trix still inside it.
  • Work It Out Wombats!: In "Sparklepants," Mr. E screams "NOOOOOOOOO!!!!!!" when a large shipment of Sparkle Oven Mitts are delivered.
  • X-Men: The Animated Series:
    • A wonderful Big No can be found in episode 4 of season 2, "One Man's Worth part 1". The first occurrence is when Bishop shouts it as Professor X is assassinated in the '50s before he can found the X-Men, followed quickly by an epic Big No from Prof. X when he vanishes from the present. The episode ends with a repeat of the opening scene — but this time, it's in slow motion for the last few lines of dialogue, including Bishop's Big No. Behold.
    • Also there's a Big "NO!" from Apocalypse at the end of the four-part "Beyond Good and Evil".
    • As well as one from Wolverine in "Out of the Past: Part 2" when Yuriko was touched by the M'Kraan Spirit Drinker.
    • In addition, there's one from Cyclops in "Reunion, Part 1" when Sinister and his gang kidnaps Jean.
  • Another Big No scene sadly fails in X-Men: Evolution, when Nightcrawler reacts too slow to stop an angry Rogue from pushing Mystique's petrified body off a cliff and has to see it shatter into a thousand shards right in front of him, shortly after finding out that Mystique was his mother. Again, the scene would have been so much better without the slow-motion yell. When recapping that episode in the introductions to later ones, they put the "NOOOO" back at normal speed. It's much more effective.

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