Used in the Teen Titans episode "Birthmark" by Raven when Slade shows her a vision of a destroyed world that she's going to create. Also used by Raven in the same episode when she refuses for a final time the party the others have planned for her birthday and destroys everything with her powers.
íMucha Lucha! is a perfect example of this. The Flea did one when his baby sister Pulgita is about to be killed by The Masked Toilet so he went inside the toilet and Pulgita beats up The Masked Toilet and at the end of the episode The Flea did it again when his scary story when he told his baby sister who he tucked her in became true when The Masked Toilet wanted his shoes and The Flea said it three times.
In The Return of El Malefico, Buena Girl say this and she was too late because of Rikochet read the code of masked wrestling backwards which set El Malefico free. Also it was The Flea's fault because he tricked Rikochet into doing it while The Flea is controlled by El Malefico and at the end of the movie El Malefico also said this because he failed to take over the world.
Plankton lets one out at the end of "Plankton!" after he ends up stuck in his computer wife Karren.
SpongeBob does this in "Pickles!" when he finds out he forgot the pickles.
SpongeBob Patrick, I'm not coming home. Patrick: I miss you! Sandy misses you! Even Squidward misses you! (Squidward is throwing himself a party at home) SpongeBob: I'm happy here. This is my home now. (Sandy drags Patrick away) Patrick: Nooooo!! SpongeBob!!
Squidward does one in "Opposite Day" when he sees Patty's car.
Mr. Krabs lets one out in "F.U.N", when Plankton steals the formula.
Employee of the Month: "NOOOO! How could I have let the quality of my work slip so much!"
SpongeBob: Mrs. Puff! Look! I've finally got my driver's license! Mrs. Puff: (takes his license and tears it up) Not even in your dreams, Mr. Square Pants! SpongeBob: Noooo!
In "Something Smells":
Patrick: Maybe it's just because you're ugly. SpongeBob: Ugly?! (sticks finger in mouth, slides it across his eyebrows) You gotta be kiddin' meh. Patrick: Let us try the reflection test. (holds up a mirror in front of SpongeBob) SpongeBob:(to reflection) Hi! (SpongeBob's reflection grabs a hammer and breaks itself after smelling SpongeBob's breath) Patrick: Uuuuuuglyyyyyyy.... SpongeBob: OH NOOOOOOOOOOOOOOOO!!!!!!!
Also used in "Bubble Buddy", in dramatic slow-motion and with added vowel screwing around with.
And used twice in "Frankendoodle."
Two of them in "New Digs", one by Spongebob when he is late, one by Squidward when Mr. Krabs evicts SpongeBob from living at the Krusty Krab.
In the movie, Plankton lets one loose when SpongeBob began zapping the mind-controlling helmets off of the Bikini Bottom residents using The Power of Rock.
Gary does this in Treats!...with a big long meow as a substitute.
"Glove World R.I.P":
Glove World Owner: Well, enjoy that hat. Now if you'll folks will excuse me, I have a rickety old theme park to close down. SpongeBob: Then the rumors are true, you are gonna close Glove World. Glove World Owner: Yep. SpongeBob & Patrick: NOOOOOOOOOOOOOOOOOO!!!!!!!!!!!!!!! Glove World Owner: I understand your concern, but really... SpongeBob & Patrick: NOOOOOOOOOOOOOOOOOO!!!!!!!!!!!!!!!
Sev Trek: Pus in Boots (the Australian CGI spoof of Star Trek: The Next Generation). Captain Pinchhard utters one of these upon hearing that Measly Cruncher is going to save the Enterforaprize for the 47th time. He stops this from happening by vaporizing Measly.
In the episode "Mayored to the Mob", bodyguard-in-training Homer practices his slow-motion dive. Making it funnier, the instructor is voiced by Mark Hamill (who starred As Himself in the same episode), who should know his Nooooos...
(Homer gives a running dive with an unenthusiastic Big No) Instructor: Well, your dive wasn't bad, but I just didn't believe your 'NOOOOOO'! You gotta sell it! Remember, your 'NOOOOOO' is what gets you your next job! Now drop and give me twenty. Homer: NNNNOOOOOOOOOOOO!!! Instructor: Better.
Used straight in episode "The City of New York vs. Homer Simpson", when Barney discovers he holds the black pickled egg that means he's today's designated driver.
Barney:(after noticing the others have normal eggs and slowly seeing he got the black one) NOOOOOO! (crushes the egg in his hand and holds his head) Homer:(coming up to him)You got the black one.
Also used straight by Bart in "Black Widower".
Seymour Skinner does three in a row in the episode "Bart's Comet", each with increased intensity; firstly, when he discovers that Bart has managed to discover a comet, something Skinner has dreamed of for years, when he briefly left Bart in charge of his telescope whilst trying to capture a less-than-complementary hot-air-balloon depicting Skinner with a giant rear that Bart earlier released ("Noooo!"). Then, he realises that in his earlier grief he's released the hot-air-balloon, letting it float free to humiliate him further ("Noooo!"). Then, a paperboy drops the morning paper in front of him, with a headline reading "PREZ SEZ: SCHOOL IS FOR LOSERS": ("NOOOOOOOOOOOO!").
Skinner pulls a similiar one season 4's "Duffless".
Done yet again in "Homer and Lisa Exchange Cross Words". Homer makes Lisa so angry that she tells him that she's going to use Marge's maiden name (Bouvier) instead of Simpson. Homer drops to his knees and screams "NOOOOOOOOOOO!" Then another girl walks by and asks if he wants to buy some Band Candy. Homer's reply: "YEEEEEEEEEEEES!"
And then there's the episode where the Flanders's get Bart, Lisa, and Maggie as foster children.
Ned: Do you reject Satan, and all his empty promises? Homer:NOOOOOOOOOOOOOOO!!!!!!!(skin burns on contact with holy water)
"Guess Who's Coming to Criticize Dinner?":
Lisa: Dad, no! [The poisoned eclair] is going to kill you! Homer: Eh, I've had a good run. (prepares to take a bite) Lisa: No! (thinks) It's low-fat! Homer: NNNNNNNNNNNNNNNOOOOOOOOOOOOOOOOOOO! (throws eclair)
And parodied yet again in an episode where Grandpa is a Sprawl-Mart greeter, and gets knocked onto an out-of-control shopping buggy.
Grandpa: NOOOOOOOOOOOOOOOOO— (cut to show that he's headed for a stack of lawn gnomes) —OOOOOOMES!
In "Homer's Barbershop Quartet", Chief Wiggum lets out one that gets twisted into a wolf-type howl at the moon.
In the beginning of the episode "The Bart Wants What It Wants" Marge threw the Olympic Torch to the Olympic Administrators and the helicopter crashed and...
Administrator 1: Are you okay? Administrator 2: Yeah, I'm ok...(looks at extinguished Olympic Torch) NNNNNNNNNOOOOOOOOOOOOOOOOOOOOOOOOOOOOOOO!!!!!!!!!!
In the "138th Episode Spectacular", alternate clips are shown including another ending as to who shot Mr. Burns. When he fingers Smithers as the culprit, Smithers gives a big no then adds, "Wait a minute. Yes."
In one episode, Bart learns that his nemesis Sideshow Bob is to be set free from prison leading to Big No. However, he learns this in the middle of class while secretly listening to a radio, prompting a rather bemused response:
Ms. Krapabbel: ... Well. Despite Bart's objections, the people of South Africa can now vote in free democratic elections.
In "Scuze Me While I Miss the Sky", Lisa' who doesn't want the town's lights on at night, lets one out as Mayor Quimby flips the switch turning on every light in town.
Many in Futurama. In particular, being a parody of melodramatic acting, Calculon the robotic soap-opera star is prone to do this sort of thing a lot.
"Bend Her" has two in one episode. Calculon is being interviewed on a talk show with Coilette as another guest (who is Bender who changed his robotic sex and invented his own country to win multiple fembot Olympic medals). When asked to set the audience up for the clip they are about to show from a new episode, Calculon responds "No, I think it's self-explanatory." The clip shows Calculon screaming "NNNNOOOOOOOOOOOO!" (while a pirateinexplicably cooks burgers on a barbecue behind him). After the clip, Calculon nonchalantly replies, "Funny story: The script called for me to say 'yes' but I gave it a little twist." Afterwards, "Coilette" ends up engaged to Calculon but has to call the wedding off before the sex change becomes permanent. After... she fakes her death of at what would have been her wedding to Calculon...
Dr. Zoidberg:(in the middle of eating and nonchalantly)I'm a doctor. He's dead. Calculon: No! NNNNOOOOOOOOOOOO! N! O! O! O!
Done again by Calculon in "That's Lobstertainment!", when Harold Zoid (Zoidberg's over-the-hill celebrity uncle) is trying to make a movie that will guarantee him an Oscar.
In "Mars University", Gunther the genius monkey decides to get a degree in business with his now only decent intelligence. causing Professor Farnsworth to shout a big no.
In "Love and Rocket", Bender dumps the crazy Planet Express ship while she, and the main cast inside her, are being chased by a large fleet from Omicron Persei 8. Cue big, shrieking no.
The above episode also has one during the beginning in an episode of the Show Within a Show "The Scary Door". A scientist builds a robot that does everything for him, including shouting "NOOOO!" when the irony of the Karmic Twist Ending sinks in.
Bender in the episode "Lethal Inspection" when he learns he wasn't built with a back-up unit.
In "The Duh-Vinci Code" when Fry accidentally destroys The Professor's Beard of Leonardo Da Vinchi only for him to find a hidden scroll in it a second later.
Professor: No! Noooooo! But possibly yes.
Played with by Bender in "Silence of the Clamps" where, after delivering a Big "NO!" as he witnesses Calculon getting clamped into a misshapen mess in front of the Robot Mafia, describes the scene in hilariously full detail in front of the chief of police, complete with imitation of said Big "NO!". When required to testify before a grand jury, he repeats his bit-by-bit exposition of the Robot Mafia's act, only to have the Big "NO!" reproduction interrupted by the prosecutor. He asks to let him finish, then lets out a brief "o" representing the end of the Big "NO!", then says that he is, in fact, finished.
Shendu yells this after ending up possessing Valmont, instead of Jackie, which was what he intended. Almost all of the Demon Sorcerers scream this when they are banished back to the Netherworld through their portals. When they banish the Earth Demon, Jade hangs a lantern on it: "Here comes my favourite part. "NOOO!""
Uncle is also fond of the Chinese version "Iyaaaa!" — usually just before smacking Jackie around the head for doing something stupid.
Parodied throughout the Drawn Together episode "Terms of Endearment", in which Captain Hero screams "Nnnooooooo!!" at least 7 or 8 times. At one point (as the camera pulls back... and back... and then back some more, to reveal just how big a problem he's facing) Hero screams the Big No three times in a row, with dramatic pauses in between.
Often used by SWAT Kats villains such as Dark Kat and The Pastmaster when the heroes defeat them.
Done straight on a fairly regular basis in ReBoot. Bob, being shot into the Web at the end of season two, Enzo, after learning about Bob's fate, Dot in "Game Over" when it appeared that Enzo had been nullified (that scene, coupled with Megabyte's calm laughter, still gives this editor chills), and said twice by Megabyte in two episodes toward the end. This YTMND does a rather good job of pointing out just how often this trope is played.
Lampshaded in the musical recap at the end of the third season:
A wonderful Big No can be found in episode 4 of season 2, "One Man's Worth part 1". The first occurrence is when Bishop shouts it as Professor X is assassinated in the '50s before he can found the X-Men, followed quickly by an epic Big No from Prof. X when he vanishes from the present. The episode ends with a repeat of the opening scene — but this time, it's in slow motion for the last few lines of dialogue, including Bishop's Big No. Behold.
Also there's a Big No from Apocalypse at the end of the four part "Beyond Good and Evil".
As well as one from Wolverine in "Out of the Past: Part 2" when Yuriko was touched by the M'Kraan Spirit Drinker.
In addition, there's one from Cyclops in "Reunion, Part 1" when Sinister and his gang kidnaps Jean.
Another Big No scene sadly fails in X-Men: Evolution, when Nightcrawler reacts too slow to stop an angry Rogue from pushing Mystique's petrified body off a cliff and has to see it shatter into a thousand shards right in front of him, shortly after finding out that Mystique was his mother. Again, the scene would have been so much better without the slow-motion yell. When recapping that episode in the introductions to later ones, they put the "NOOOO" back at normal speed. It's much more effective.
Optimus Primal gets quite a few in Beast Wars, most notably in the cliffhanger of the first season finale, and when the Axalon is destroyed in season 3.
Inverted with Megatron, as he usually says "Yesssssssssssssss."
Last episode of Beast Machines. Megatron's final word? "NOOOOOOOOOOOOO!"
And in Armada, Optimus Prime gets one of these when Smokescreen dies.
Megatron later gets one when Starscream of all people is killed by Unicron.
Parodied in the "Bee in the City" script reading of Transformers Animated, with David Kaye using his Beast Wars Megatron voice (to enthusiastic approval from the crowd). Upon his defeat, he vows to return, ending with a big "YEEESSS!"
In Animated, the Autobots discuss having to leave, and Bulkhead wonders how Sari (human sidekick) will take it. Bumblebee says she's a big girl and can handle it. Cut to Sari screaming NOOOOOOOOOOOOOOOOOOOOOOOOOOOO!! at the top of her lungs.
Not to mention the episode "Nature Calling" when Prowl smashes Bumblebee's only battery, rendering him unable to see movies in the middle of nature. His "NOOOO!" was truly epic.
Littlefoot gets one of these in The Land Before Time IX: Journey to Big Water, as he watches Mo lead the swimming sharptooth away.
Used in American Dad! by either Stan, Francine or Hayley when they believe Steve and Roger were killed by a lightning-struck treehouse. Rather effective due to how strangled and broken it sounded. Steve also gets a particularly good one.
In "Stop Team Go", after using hundreds of mooks to destroy the gurken jar, Dr. Drakken puts the jar under a big laser beam, and he fires it. The result was a big crater on the floor, and the jar was unharmed. Cue Big No.
In another episode, upon learning that her very annoying younger twins have been skipped ahead and are now in the same high-school as her, Kim lets out a big no powerful enough to knock letters of the school sign out front, while she's indoors.
Ron has a couple throughout the series (including a truly epic one in "A Very Possible Christmas" (Season 2, episode 16).
In Brain Invaders, Barriss Offee screams "NO!!!" as she gets infected and possessed by a Geonosian Brain Worm offscreen.
Anakin pulls off this trope twice in the Mortis Trilogy. First, when the Son of Mortis kills Ahsoka, and second when when the Son shows him what he will become in Revenge of the Sith.
ARC Trooper CT 27-5555 "Fives" screams one when Hardcase tells him that he's going to sacrifice himself to destroy a Separatist Supply Ship after the shields to the reactor core were raised.
Obi-Wan does it a couple of times: first, a quick one when Zygerrian Prison Warden Keeper Arguss activates the control panels to the slaves' quarters to drop them all into the pit of doom below. The second time is right before Savage Opress gives Adi Galia the finishing stab with his lightsaber after impaling her with his horns.
Judge: You, Mojo Jojo, are hereby sentenced to teaching a knowledge extension course at Townsville Community College!
Mojo Jojo: NOOOOOOOOOOOOOOOOOOOOO! By which I mean I cannot do it.
"Fallen Arches": Captain Righteous and Lefty confront the Ministry of Pain. The Captain lets out a big no as he runs towards Lefty who just hurt his hip. However, he hasn't reached his sidekick yet so he shouts it again.
The comic book story "Steal A Meal" (Cartoon Network Block Party #26) has the girls doing a tearful Big "NO!" upon learning that after all the breakfast cereal in town had been stolen the only thing left was oatmeal.
"Mime For A Change": Bubbles performs a great Big "NO!"
Parodied in the Space Ghost Coast to Coast episode "Boo Boo Kitty", when Space Ghost's pet cat, actually a large balloon, slowly drifts towards a large safety pin. He screams NOOOOOOOOOOOOOO!, runs out of breath, inhales, and starts again. After the balloon deflates, he promptly ignores the others when they suggest he could have easily saved Boo Boo Kitty from its untimely death.
Played for laughs in Metalocalypse, where Big Nos are Nathan's catchphrase, and he lets them out quite regularly. They are usually accompanied by the camera flying down Nathan's throat. One example comes when Nathan lets out a NOOOOOOOOOO! upon Nick Ibsen revealing Dethklok's parents.
Nick Ibsen: You are all going to be reunited with your families. (a panel opens up, revealing the families of each of the five band members) Nathan Explosion:NOOOOOOOOOOOOOOOOOOOOO!!! You're going to pay for this! Nick Ibsen: Pay for what? Journalistic integrity?
The bit on flying down the throat comes from the episode "Murdering Outside the Box". When Nathan learns that someone is embezzling from the band, he suddenly goes into the Big No sequence, and from the "down the throat" bit the show goes into the opening.
It also happens when Nathan, after having death-growled the entire works of Shakespeare to sell as an audiobook, learns that an extremely-inebriated Pickles "like...TOTALLY forgot to hit record, man." The strain of recording the growled version of "Hamlet" having taken its toll on Nathan's larynx, the Big "NO!" was rasped rather than screamed.
Ace Lightning: The characters in this mixed media show are based on superhero stereotypes, so this was going to show up sooner or later — except that it's not one of the CGI characters that do it — it's a human, in the last episode, when he thinks Ace has been shot.
Also used in some episodes, such as the one episode when Vega yells this after Blanka attacked Vega's face and after Vega looks at his reflection in the water.
In another episode, Fei Long yells this twice.
During "The Strongest Woman in the World," a young Chun Li yells this in a flashback as she witnesses M. Bison killing Chun Li's father.
The final one is delivered by Cammy in the final episode when she realizes that everything that M. Bison said was lies.
In Freakazoid! episode "House of Freakazoid", Freakazoid is pressured to pursue a villainess through a sewer, but repeatedly refuses because he hates the smell of poo gas.
Freakazoid: NO! A big N, followed by a little O: no!
Parodied in The Fairly Oddparents "Wishology: The Exciting Middle Part", which ends with a big "Dagnabbit!"
Played straight in the Fairly Oddparents: Shadow Showdown video game; Timmy shouts it after his TV breaks in the introduction, and again upon seeing he's missed the Crash Nebula season finale at the end.
And parodied in "Pixies Inc", where, after being foiled, the Pixies let out a big no—with no emotion.
Cosmo and Jorgen in "Cosmo Rules", when they learned they're cousins. Their Big "NO!" lasted nearly 24 hours as the scene showing the sun setting and rising again suggested and then Jorgen fainted and Cosmo added ..."WAY!" Cosmo was enjoying the idea and even planned cousingly activities for him and Jorgen. Albeit not all of them were fit for male cousins.
Chester also did this in "The Boy Who Would Be Queen:" when A.J. fell in love with Timmy's girl form "Timantha."
In the last episode of the third season of The Venture Bros., Henchman 21 indulges in a Big No when the Monarchmobile explodes with Henchman 24 trapped inside, but is interrupted when 24's severed head hits him in the chest.
In Brother Bear, Kenai lets out the distant, bird-disturbing version of a Big No when he discovers he's been transformed into a bear.
In Batman Beyond, Derek Powers accidentally inhales a big gulp of his own nerve gas, and in the same second it takes him to realize what has happened to him, makes an appropriately horrified scream to this effect.
An episode of Family Guy has Peter be really upset over the loss of his pet parrot.
Peter again in "McStroke" when his mustache burns off.
The Donkey Kong Country episode "Buried Treasure" contains this exchange when Klump's video conference interrupts K. Rool's computer game:
Klump: General Klump reporting, King K. Rool sir! Y'read me, your high 'n mightyness?... Looking a little pale there, chief! K. Rool:NNNNNNNNNNOOOOOOOOOO!!!!!!!!!!!!!!!!!!!(smashes the computer) Klump:(calling on the phone) Chief? You there, chief? Seems we had some sort of interference.
In the episode "Cat Orgy", when Skyler discovers his trashed guitar.
The episode "Jewbilie" does it four times in a row: First Kyle when Kenny makes a Heroic Sacrifice to stop Haman, then Haman when Moses escapes the conch shell, then anti-semetic Rabbi Garth screams it twice when he realizes his plans have been foiled.
Mormon: We were just about to do a play about how much stealing hurts you deep inside. Come join us. (the Mormons lift Saddam over their heads and carry him off) Saddam: No! NOOOOOO!
"Something You Can Do With Your Finger" parodies the Star Trek: First Contact example above, when Randy prevents Stan from being in his boy band. Incidentally, they actually use the sound clip of Picard's breakdown from the movie for that scene.
Stan: Why can't I be in a boy band? Randy:(firmly)Because I Said So. Stan: Cartman said we're going to perform at the mall at three o'clock! My friends are gonna be pissed off at me! Randy:(still pretty controlled) Let them be pissed off. Stan: Dad, I don't understand. Just let me do this one thing and then I'll... Randy:(inexplicably losing it)Noooo! NOOOOOOO! (smashes a glass dresser with his head)
In "The Last of the Meheecans", Randy does it again when he's confronted with what happens when Mexican immigrants performing menial labor all decide to move back to their birth country in a fit of nostalgia, using the same Darth Vader soundclip from Revenge of the Sith.
In "Marjorine" Linda Stotch lets out several blood-curling screams of "NO" when she thinks her son Butters has committed suicide by jumping off a building. She does it again at his funeral while throwing herself on his casket.
Rocko's Modern Life: Rocko and Heffer are on a bus tour of Paris with a bus driver who is determined to keep his passengers on the bus at all costs, so much so that he chases them around the city after they escape. By the time the two board a plane home, the bus driver delivers an epic BIG NO as the two fly off into the sunset.
Spider-Man: The Animated Series: Blade lets out a particularly narmtastic one of these in the season four episode, The Vampire Queen. And Spider-Man gives a particularly haunting one upon the death of the Mary Jane Clone.
Owen says a big no when a box of chocolates gets knocked into the water.
Also, Alejandro in the finale of the 3rd season gives out a Big No after he is put in a robot suit because he was burned by lava and trampled by most of the contestants. Al lets it out when he asks Chris if the prize money is safe, only to find out it was burned in the volcano. It's hilarious because it parodies the cheesiest moments in Star Wars history (which is seen in the picture above).
Episodes "Pretty Poison" and "Eternal Youth", each near the end, have the villainess Poison Ivy saying this trope.
The episode "His Silicon Soul", near the end, has the cyborg Batman saying it after the real Batman fell to his death before Cyborg Batman committed suicide. Then it's revealed that Robin and Alfred see a light below which is Batman himself, who survived.
Used in the episode "Mad Love" by Harleen Quinzel after The Joker gets captured again and taken away from her which helps to show us her Sanity Slippage.
This exchange from "Princess, I Shrunk the Marios":
Koopa: Know what I'm gonna do with you buttinskis? Mario: Say you're sorry and let us go? Koopa: NO!! I'm gonna lock you up for two hundred years!
Koopa lets out yet another in the Super Mario World episode "Rock TV" when he's about to get crushed by his giant television.
In The Adventures of Jimmy Neutron: Boy Genius Jimmy gets one when he travels to the future and he discovers that his future self married Cindy. And when it comes back from commercial, we discover that he screamed for four minutes.
Sheen lets out one in the interstitial shorts after his Ultra Lord action figure gets stuck in a tree. Also lets one out in "Ultra Sheen" when he witnesses Ultra Lord's defeat at the hands of Robo-Fiend inside the video game.
Sheen lets out another in "Journey to the Center of Carl" after Jimmy tells him to pretend that Ultra Lord got canceled.
Hugh does this in "Attack of the Twonkies" when Judy takes Flippy from him and throws him to Jimmy so he can power up his rocket and send the giant Twonkie back into space.
A Tom and Jerryshort revolved around a duckling imprinting on Tom and Tom taking advantage of this to attempt to cook the duckling. Near the end, the duckling realizes that Tom intends to eat him and prepares to take a long walk off a soup spoon into a bubbling pot, "If my nice mommy wants a duck dinner..." Tom utters a Big No as he catches the duckling. A "No!" made all the more impressive by the fact that Tom and Jerry usually don't have speaking voices....
Happens quite a lot. One time in particular, when Mandark forces Dexter to shut down his laboratory.
In "Jeepers Creepers, Where is Peepers," the transforming Peepers yells this when he sees Dexter and Koosalagoopagoop being attacked by Hokochu before breaking out of his container.
The Ice Cream Man, after Dexter asks if he's got change for a hundred. This is after the Ice Cream Man explains that he hates Dexter for paying in pennies.
The Death Kwon Do instructor in Regular Show gets three rather nice ones. First when he discovers Rigby stole the instructions to learn the Death Punch of Death, only to be interrupted by a student explaining the toilet was 'Death Kwon clogged', leading to another Big "NO!". Then finally, when he discovers that Mordecai stole the Death Jump and Death Dump instructions, he lets out another Big "NO!".
Scaredy Bat lets one out in an episode of Ruby Gloom.
Spike does this twice in "A Dog and Pony Show", both times coupled with Skyward Scream: the first when Rarity is kidnapped, and another when he realizes he and the other ponies have gotten themselves lost in the Diamond Dogs' mine.
Which is amusing considering this is the first episode to feature the voice of Scott McNeil, who does a similar Big NO! in Gundam Wing.
In "Stare Master", Fluttershy's reaction to Applebloom suggesting the Cutie Mark Crusaders try to get coal-mining cutie marks is a very emphatic (especially for her) "NO!"
Scootaloo does this in "Cutie Mark Chronicles", when Fluttershy suggests singing another (cutesy) song.
The Cutie Mark Crusaders do this at the end of "Hearts and Hooves Day" when Big Macintosh and Cheerliee make the Crusaders think the two of them are still under the influence of the love poison.
Several in "Games Ponies Play": first, we see Rainbow Dash give one in a flashback after she learns that Cloudsdale won't be getting the Equestria Games this time. A minute later Pinkie does one that seems to come out of nowhere until she explains that she was just answering Twilight's question ("Are we going to let Cadance down?") Later, when a panicked Rainbow Dash asks, "We got the wrong pony?!", Pinkie screams, "NOOOOOOOOOOOOOOOOOOOOO!" - before correcting herself to a Big "YES!" in exactly the same tone.
By Haman (Mr. Lunt) in Esther... The Girl Who Became Queen when he finds out he's getting banished to the Island of Perpetual Tickling.
Two by Don Quixote (Archibald Asparagus) in Asparagus of La Mancha, one when he wakes up from his first bad dream, one when Poncho (Mr. Lunt) weans him off the salsa, due to finding out that Don's addiction to his super-spicy salsa is causing the bad dreams.
By The Bad Apple in Larry-Boy and the Bad Apple, when her webs dissovles by using water.
By Archibald Asparagus in the silly song The Biscuit of Zazzamarandabo, when everyone needs to take a potty break.
Also by Larry in the silly song Sippy Cup combined with Eat The Camera when Jimmy Gourd explains why Larry needs a sippy cup.
Princess Sally yells it in Sonic's bad dream when she is roboticized in Sonic Sat AM, episode "Sonic's Nightmare".
Uttered by Kabuto at the end of Tokyo Mater as a result of him losing to Mater in a drift race and is about to have all of his modifications pulled off his body by all of the other cars in Tokyo, who then laugh at him when Kabuto is finally naked.
Recess uses this a number of times, a few being "No...no...NOOOOOOO!!!!!":
"The Break In": Towards the end, by T.J. when he just broke himself out of detention and onto the playground, just as recess ended for the day.
"The New Kid": By Gus when his name is taken away and must be refered to as "New Kid"
"The Experiment": By T.J. and Spinelli when they find out they have to kiss each other to see what the effects were on the opposite sex.
"The Legend of the Big Kid": By Vince when he and the gang are looking for T.J.
"Mama's Girl": By Spinelli when no one will stop taunting her after she accidentaly called Miss Grotke "mama".
My Life as a Teenage Robot: Vexus at the end of "Escape From Cluster Prime", when Vega and her friends give Cluster Prime back their golden chips. Also, used in "Puppet Bride" by Mrs. Wakeman (along with a flying tackle) during a wedding between Little Acorn and Jenny.
Happens quite a lot on Adventure Time. In "Wizard Battle", Finn has to win a contest in order to win a kiss from his love interest, Princess Bubblegum. When one of the other contestants almost wins, he lets out a Big No that reaches the level of a Sonic Scream.
Finn also lets one out in "Mystery Train" when he sees that Jake has seemingly been killed by the Conductor.
In the first episode of The Hub's Pound Puppies, adoptee-of-the-week Yipper lets out a cross between this and a howl of despair when it looks like he's missed his chance to get adopted by his "perfect person".
Julie does this in the Motorcity finale when she thinks Mike was killed in the self-destruction of his car.
In an episode of Camp Lazlo, Scoutmaster Lumpus does it when Slinkman says that he can earn his "Handy Helper Badge" if he can...gasp!...help someone!
In the first episode of SheZow, Guy has this reaction once he learns that his aunt's magic ring won't come off his finger and that he would be forced to carry the duty of SheZow for the rest of his natural life. This inadvertently triggers his sonic scream power and he destroys the mirror guarding the entrance to his aunt's secret lair.
One episode of Celebrity Deathmatch has Elizabeth Hurley and Anna Kournikova fighting atop a cage filled with horny teenage boys. Hurley manages to pound Kournikova into the cage, followed by a series of limbs and carnage flying from the top. Hurley assumes that the boys have ripped her opponent to pieces, until a pair of hands grab at her feet from beneath.