- Acceptable Targets: Don't expect the military to come across well in this web series, especially given that Armed Farces is one of the main sources of comedy.
- Big-Lipped Alligator Moment:
- Episode 28.5, "The Last Episode Ever". It first aired on April Fools Day.
- Episode 45.9, "The Greatest Episode EVER". An episode made entirely with paper cutouts by Gus Sorola while the rest of Rooster Teeth was out of town.
- Crosses the Line Twice: Much of the series' humor, though special mention probably has to be given to Private Jimmy's absolutely hilarious Cruel and Unusual Death.
- Ensemble Dark Horse: Viewers of Out of Mind loved Delta and York. And both Sister and Junior were very popular despite only having starring roles in Season 5.
- Epileptic Trees: And some are coming true.
- Family-Unfriendly Aesop: After a hundred episodes, Church takes a moment to reflect on how he's learned that it's wrong to hate people based on arbitrary political or military delineations. Instead you should strive to "despise people on a personal level." While not necessarily a great moral, it still rings true to an extent in that one should not mindlessly hate just because they were told to.Church: You should hate someone because they're an asshole, or a pervert, or snob, or they're lazy, or arrogant or an idiot or know-it-all. Those are reasons to dislike somebody. You don't hate a person because someone told you to. You have to learn to despise people on a personal level. Not because they're Red, or because they're Blue, but because you know them, and you see them every single day, and you can't stand them because they're a complete and total fucking douchebag.
- "Funny Aneurysm" Moment:
- After learning of Private Jimmy's true fate in Season 14, Tucker and Church's conversation about him in "A Shadow of His Former Self" is subtly horrifying.
- Sarge's hilarious Blood Knight tendencies, Insane Troll Logic, and habit of shooting/trying to kill poor Grif are all rather uncomfortable to watch as not only would Season 14 display him as being so crazy that he killed his own C.O. when being picked out as the Blood Gulch Red Team's sergeant since he thought he was a Blue in disguise, but later seasons (most notably Revelation, Season 15, and Singularity) would start to take a more critical and tragic view on how much of a negative impact being in the military for so long has had on Sarge's psyche.
- Captain Butch Flowers dying of an allergic reaction to some medication is this twofold; the first reason is that we find out this was almost certainly Gamma torturing Church For the Evulz in Season 10, and the second is due to Monty Oum's tragic death from a severe allergic reaction after surgery in 2015.
- Most things about Captain Flowers become this in hindsight after Seasons 10 and 14 reveal that he's actually Freelancer Agent Florida, a ruthless sociopath who barely batted an eyelash as Sarge killed his subordinate Lemons (even cheerily thanking him for finding the perfect candidate as Lemons pleads for help over the comm), and forcefully implanted the Alpha A.I. into Private Jimmy, completely shattering the image of A Father to His Men he had up until then.
- When the bomb inside Church is about to be activated, Tucker attempts to kill him in hopes of destroying the bomb. Church objects to that, not caring about the people around him in the slightest. This attitude comes to a change later, as performing a Heroic Sacrifice with an uncertain hope of success is exactly how Church meets his end twice over.
- Tex not showing any hesitation to kill Grif when asked to, once Season 15 reveals that it wouldn't be the first time she horrifically killed a simulation trooper in orange armor with no remorse (though her using Omega at the time then admittedly complicates the situation).
- While threatening Andy, Tex emphasizes how he will be just a memory after she detonates him. The amount of irony is staggering seeing what becomes of Tex after both Reconstruction and Revelation.
- Caboose's cheerful shout of "Connecticut!" is this when Tex and Wyoming meet each other again, now that the audience knows that Connie was one of the few Freelancers Tex had any kind of emotional bond over. Furthermore, Connie's death had the greatest impact on her out of anyone and helped kick-start her HeelFace Turn.
- Most of the second half of Season 4 and all of Season 5 portrays Church as on the verge of reaching his Rage Breaking Point as he struggles to keep Blue Team at least partly functional despite the world seeming to fall apart around them. This is largely Played For Black Comedy at the time, but The Project Freelancer Saga would later reveal that the Alpha A.I. was successfully tortured past the brink of insanity multiple times by having him be Forced to Watch as the people he cared about most died horribly and he was told that it was all his fault. So in essence, Church is pretty much going through his worst possible nightmare all over again throughout the last portion of The Blood Gulch Chronicles - and he doesn't even realize it.
- When Grif's Sister appears in Season 5, her having run away from home to be with him in the military is Played for Laughs and Hand Waved away at the time as the result of her being The Ditz. Not only would later seasons take a more serious look back at the Grif family (with Season 13 claiming that Grif was The Unfavorite and he was Promoted to Parent due to the siblings' parents being utterly despicable), but that (as revealed in Singularity) Sister actually ran away from home out of guilt for accidentally burning down her and Grif's childhood home.
- While Omega's absurd plan to conquer the universe with Wyoming and Gamma's help on the surface can at least be laughed off as the result of the two's manipulations on their own, later seasons would imply that they were still working for Freelancer Command all along (with Tex/Allison instead being the Rogue Agent) and this was actually Project Freelancer's best plan to stop the Great War.
- Growing the Beard: It was during Season 3 that the series slowly began to head towards a plot-driven structure with some moments of genuine Character Development. This was dialed down a bit in the fourth season, but soon returned in the middle and would stay that way for the rest of the arc's run.
- Harsher in Hindsight:
- Church discussing battle tactics and strategy with Captain Flowers during the Season 3 flashbacks is a lot sadder after Season 9 would show that the Alpha was used to help plan out the Sarcophagus heist that would ultimately result in its horrific Cold-Blooded Torture. In essence, the Alpha overlooking battle plans is the exact thing that got him stuck in Blood Gulch in the first place.
- During Season 3 and when Church is traveling through time, he meets up with his past self from Season 1 when he's going through his first death, with Future!Church awkwardly telling his past self that "You should rest now." This is really hard to watch now after Epsilon-Church Mercy Kills Tex in Season 9 after she makes it clear that she's tired of being brought Back from the Dead and just... well, "wants to rest."
- The time loop shenanigans in the aforementioned season also have Church giving a heartfelt apology to Tex for failing to be a better person to her than he should have been, which is pretty depressing to see after later seasons show how messed up their romance really is and how this can be seen as the Alpha subconsciously trying to apologize to Beta for him having doomed her to always fail at the last second.
- Hell, pretty much all of Church and Tex's Slap-Slap-Kiss dynamic becomes this once later seasons show how they're actually both Loving a Shadow, as their entire relationship is only a reflection of the Director and Allison Church's romance.
- In "Biting the Hand", Tex briefly recounts the events of the Out of Mind miniseries to Church, with the latter asking if Carolina was with York when she met up with the latter. While it's just a minor detail at the time, it's rather sad to see after The Project Freelancer Saga would reveal that Church can't remember almost anything from his time as the Alpha A.I.. That means one of the only things he still remembered from that period was the daughter of the man he's based on.
- Tex gently whispering "Goodbye" to Church as she lifts off on the Pelican in "Why Were We Here?" is pretty tragic to watch after The Project Freelancer Saga would delve into how the Director (and, to a lesser extent, Carolina) never truly got over the death of Allison Church because they Never Got to Say Goodbye.
- Church famously hates everyone on his squad (not to mention everyone not on his squad). To be fair, the people he has to work with aren't what anyone would call class-A soldiers, and Church usually has to play the role of babysitter. Still, this was one of the most charming aspects of his character. Then comes Season 10, where late into the season the Blood Gulch Crew refuses to help him and Carolina track down the Director, Church (now Epsilon) tears into all of them, telling them that they've been a pain in his side for his whole life. It is significantly less funny there.
- Hilarious in Hindsight:
Church: I've got half a mind to kill you, and the other half agrees!
- The third episode has Grif (voiced by Geoff Ramsey) poking fun at a Noodle Incident Gilligan Cut involving Simmons (voiced by Gus Sorola) refusing to go to the Vegas Quadrant. One of the Rooster Teeth podcasts revealed that this was Based on a True Story.
- A panicked Grif begging Tex to not hit him in the face once he insults her is much funnier to watch after later seasons have all iterations of Tex focus almost solely on giving Groin Attacks to the poor guy. Be Careful What You Wish For, Grif!
- Caboose claiming that Church is a "gay robot" after learning of Tex and Church's relationship in Season 1 is absolutely hysterical after Season 14's "Get Bent" would reveal that Epsilon-Church was perfectly fine being with a male version of Tex while stuck in the Memory Unit.
- Church's attempted Pre-Mortem One-Liner, after The Reveal at the end of Reconstruction, considering he's partnered with Tex to take down O'Malley.
- Also, Church's exasperated cry of, "Holy crap, who is running this army?!" becomes utterly hilarious when we find out the person running the Red and Blue Armies is actually Leonard Church!
- When performing a hostage exchange near the end of Season 2, Caboose calls Donut "Major Cinnamon Bun." Keep in mind that Season 2 of Red vs. Blue aired three years before the first Adventure Time short even aired.
- Caboose's statement of how "time is made of circles" is pretty hilariously prescient after Singularity would reveal that black holes in the RvB universe loop backward in time to before the Big Bang.
- In Season 4, Tex sarcastically wonders aloud that if she kills Church while he's a ghost than if he would "come back as a ghost of a ghost." That's essentially what happens in The Recollection: Church is killed in the finale of Reconstruction, and is revived in Recreation via Epsilon and Caboose's memories of him.
- Sheila's appreciation for her new Pelican-class dropship body is quite amusing when it turns out she's almost certainly based off of the Freelancer Dumb A.I. F.I.L.S.S.
- "No, another ship, then that ship left and this ship crashed in the exact same spot. " Come Season 17, and the revisiting of this moment has something very reminiscent of this sarcastic answer: Sister's Pelican doesn't land on Donut, only for another one to appear and crush him.
- The Bundeswehr is calling its newest Awesome Personnel Carrier the "Puma".
- Jerkass Woobie: As noted below, Tex. Church also starts to fall into this once it's shown how he's a Love Martyr that completely fails to save his girlfriend from the hands of an evil A.I. despite his best efforts and is full of immense self-loathing issues.
- Never Live It Down: Caboose really only killed Church once with a tank (and that wasn't even entirely his fault). He sort of killed Church while the latter was possessing Sarge, but as a ghost form, he couldn't be killed, and Sarge got better. Yet they won't stop giving Caboose crap about killing his own men. Though granted, this may be because this won't be the last time that this happens.
- One-Scene Wonder: Private Jimmy, who only appears in Church's flashback in Episode 10 and is used for a hilarious Death as Comedy joke. He was even brought back for the Season 14 Origins Episodes, though his reappearance goes through a significant Cerebus Retcon.
- Rescued from the Scrappy Heap: Downplayed with Caboose. When he started out, he was just a slightly slow Deadpan Snarker with a rather flat affect that didn't make him very memorable. However, after the writers Flanderized him into becoming a Cloudcuckoolander Ditz, he became easily the most popular character out of the entire series.
- Robo Ship: Episode 28.5 has this.Doc: Everyone! I have something to say! Tucker and I are in love, and we're leaving!
Tucker: That's right! We're getting married and there's not a damn thing you can do about it! Not even you, French-Speaking Lopez!
Lopez: [Mister Tucker, you promised your love to me!]
- Seasonal Rot: While Season 3 had received some praise for its more plot driven structure, it was also criticized for downplaying the sharp dialogue and character interplay that made the first two seasons so enjoyable. When Season 4 returned to the show's original formula, many fans were pleased.
- Signature Scene: The "You ever wonder why we're here?" Book Ends from Episodes 1 & 100.
- Stoic Woobie: Later seasons reveal that Tex had been one of these the whole time. She knew exactly the kind of torture that Church had been through, and was simply trying to watch over and protect the broken remnants of someone she had loved, and had demolished an entire military base/spaceship trying to save.
- Overlaps with Jerkass Woobie to an extent when you realize the reason she acts like such a Jerkass to Church. Every time she sees him, she's reminded of her failure and that the Alpha will never be what it once was. No wonder she can barely stand to be around him, especially after the Alpha Took a Level in Jerkass following the attempted breakout. Could also be a facade since she likely acted so cold to him in order to keep him distant or from becoming too attached to her, since it could, and did, lead to him discovering what he really was.
- They Wasted a Perfectly Good Character: Grif's Sister (whose first name, Kaikaina, isn't even mentioned in the show itself until Season 15) has barely any effect on the story, and disappears almost entirely after Season 5, despite all the fun gags or plot complications that could be built off Grif having a sibling on the other side.
- The Woobie: Both Donut and Caboose. Sure, they both might be as dumb as posts, but they're both still just so adorable and sweet, which makes it all the sadder when things go horribly wrong for them.
- Ugly Cute: Junior.
- Values Resonance: In a weird sense. There's something rather remarkable about The Blood Gulch Chronicles giving such a dim view on militarization and war for an American web series that only started to air less than two years after The War on Terror started.
YMMV / Red vs. Blue: The Blood Gulch Chronicles