Join Scooby and Mystery Inc. as they vacation in the land Down Under where they attend a music festival held at Vampire Rock. Unfortunately, a mystery rises when they learn a legendary Australian creature called the Yowie Yahoo is kidnapping musical acts and turning them into vampires. Masquerading as a heavy metal band, the gang must survive a trio of rock-and-roll vampires if they want to crack the mystery surrounding the legend of the vampire.
- Arbitrary Skepticism: Despite the gang recalling having met the Hex Girls before, which happened in Scooby-Doo! and the Witch's Ghost (where Mystery, Inc. dealt with the real witch's ghost), Velma remains adamant that there are no real vampires. While the vampires of this film are revealed to be another "Scooby-Doo" Hoax, Velma's refusal to consider that vampires might actually exist is still pretty jarring.
- Art Shift: This movie marks the first change in the series. Starting here the films go for a flatter old-school revival art in the process departing from the darker shading and effects used previously. It also abandons the more modern cast designs used by the first four movies.
- Call-Back: The return of the Hex Girls from The Witch's Ghost. The gang mentions that they've met before, likely referring to the events of the movie.
- Deadly Euphemism: Parodied. When asked what happened to the Bad Omens, something even Mystery Inc. couldn't figure out, Lightning Strikes says that they "sent them and Matt Marvelous on diving trips to the Great Barrier Reef." Stormy Weathers immediately clarifies that they were sent on actual diving trips, all expenses paid.
- Faking the Dead: It turns out that Wildwind faked their own disappearances to increase their own mystique and further their careers. The whole "Vampires" thing and their disguises of Russel and the band Two Skinny Dudes were a means to get around the Vampire Rock festival's "no repeat bands" rule by kidnapping the other bands and bribing them to drop out so they could close out the festival in the place of the nonexistent Two Skinny Dudes, who would've won by default.
- Hello, Nurse!: Some Australian lifeguards take notice of Daphne in her bikini and start flirting with her, which makes Fred jealous.
- The Legend of X: The film has the subtitle Legend of the Vampire.
- Lighter and Softer: Its the first to return to the lighter tone than that of the previous four movies. It's especially noticeable with The Hex Girls. Their demeanors are lighter and Thorn was redesigned to be less "scary".
- Ms. Fanservice: Daphne's seen in a bikini a couple of times, one of which is when she and the rest of the gang are hanging out at Bondi Beach in Sydney.
- Musical Nod: The movie brings back a lot of the soundtrack and sound effects from the older shows, which the franchise hasn't since repeated.
- Mythology Gag: In the opening sequence introducing the gang, several shots from the original 1969 opening are recreated.
- The Gang pose as a band to enter the contest as a ruse for their investigation, and one of the other bands' members snarkily asks who'd ever heard of a band with a dog in it. Early concepts for the original Scooby-Doo cartoon depicted the Gang as a traveling rock group, with a bongo-playing sheepdog as their Team Pet.
- Role Reprisal:
- Casey Kasem went back voicing Shaggy for the rest of the Scooby-Doo direct-to-videos series until the thirteenth installment and both Heather North and Nicole Jaffe reprised their roles of Daphne and Velma, respectively. Fred and Scooby still have the same voice actor.
- The Hex Girls return, with Jennifer Hale, Kimberley Brooks, and Jane Wielden once again voicing Thorn, Dusk, and Luna .
- Sadly Mythtaken: The Yowie Yahoo is actually the name of Australia's version of Bigfoot. The real vampire of Australia is the Yara-ma-yha-who, a small, hairy and red froglike imp with a huge head and octopus suction cups for fingers and toes and prefers to live in fig trees.
- "Scooby-Doo" Hoax: The first film in the series to return to the original format with the monsters really being criminals in masks.
- Vampires Hate Garlic: A chase sequence has Scooby and Shaggy try to get rid of the Dark Skull vampire by having him eat spaghetti topped with garlic salt. It doesn't work. Of course, he wasn't a real vampire to begin with.