A-Cup Angst: One player suggests that Ruby should flash Tom to get him to cooperate with her escape, but Weaver replies with "Oh no! Unfortunately for all parties involved, Ruby is flat as a board! That probably won't work."
Face-Revealing Turn: Jesus Christ there's someone behind that mirror! Oh well, maybe this time they'll be fr HOLY SHIT
Mistaken Identity: Was called the Hound until a note mentioning Subject #2 aka Maddie appeared, and was referred to as that until another note which revealed that Maddie had died and never came back, but that same note revealed her real name as Daisy, which has confirmed to be her real name.
Identity Confused: At first, Daisy goes by "Maddie" and later storms away, coming back wearing a pig masknote (likely referencing the lost patient "Lucy," a supposed man-bear-pig creature), calling herself "Ruby" and that everyone should trust her because of that.
No Fourth Wall: In "NotWeaver's" mini-installment, Daisy directly converses with /tg/.
Affably Evil: Subject 6 is possibly the creepiest character of them all. He's dangerous, violent and apparently delusional, but is also capable of having a nice friendly chat with Ruby without harming her, as well as providing her with an important key.
And fans would think this to be true up till the game ended. But one of the ripped notes thrown into the water proves otherwise — that the character they thought to be '6' turned out to be Subject FIVE.
Tom was Subject 6. Back before things went to hell, he began acting violent FIRST, thus the "dangerous" description he was given. This also explains why he busted up so much stuff on the player's whims.
Determinator: He's shown to be very much in love with someone named "Lucy" and has been waiting for her probably as long as he's been without her. He has even become so deluded that he believes Ruby is "Lucy".
Badass Pacifist/ Technical Pacifist: Even though he is the most feared creature in the facility, and even being known to charge around the facility with a freakish spear-hook, he is never actually seen harming anyone. More likely, he gets his fearsome reputation by sheer intimidation, which, for him really isn't that hard. Word of God even states that Ace is not to harm (or at least kill) any of the patients or other staff, himself, due to his mind being controlled by the CJOPAZE.
Though, in one of Weaver's half-discontinuities, he is seen stunning Ruby with an electric prod.
Battle Aura: Near the very end, he is outlined—presumably glowing—with a blood red color.
The Bad Guy Wins: As Word of God says: "You know, from Red's point of view, he won. He never got caught. He never got beaten. He never gave up. And he exited on his own terms. And most of all, he died with a smile on his face."
Hero of Another Story: Because saying "The Bad Guy Wins" implies that Ruby's and Tom's success depended on Red's defeat and vice versa. Thankfully for all parties involved, this wasn't the case.
Unwitting Instigator of Doom: It was originally his idea to obtain and administer the new "cure" which had unintentionally caused the unfathomably catastrophic meltdown of the facility.
Villain by Default: Besides Tom whom ate the tank fish, Red is the only one in the story blatantly established to be a predator, by nature.
Though, he most always seems to be in control of this—lampshaded by the cereal—and this is handwaved after he states that he was "just kidding" after threatening to bite Ruby's throat out.
Younger than They Look: Red is occasionally mistaken to be much older than he actually is; often due to use his of the cane, which was sort of implied as an elderly crippling. Word of God states that he is just as old as Ruby and Tom.
Nice Guy: Apparently, he had been a very nice man—er, fox—before his first suicide. By "Maddie's" accounting, he even told good jokes.
Sharp-Dressed Man: Often portrayed as such in fanart. note (Furthermore, is often his general perception among the fandom — Possibly canonized when Weaver posted the second image in his Daisy Quest thread)
A male squirrel and the head doctor at the facility.
Madness Mantra: Filbert would like you to know he is clean. And he needs to cut the bad out of you.
Manipulative Bastard: As seen when Ruby first meets him, he can easily fake sympathy and gain pity without even trying. He can also fool others into thinking he's a competent doctor and most often manipulate them into doing nearly anything he wants.
Only a Flesh Wound: He had been assaulted hard by Ruby's barbed-wire glove, but gives almost no recognition to this injury and continues his attempts to make Ruby acknowledge his commands and wild rantings.
An unnaturally-tightly leather-bound scarecrown/ dummy bolted to a rotating pivot that seems to always be staring toward the players.
Artifact of Doom: As stated from the last of Filbert's notes, the Dummy—along with several other malevolent artifacts—were retrieved from the recently excavated, lost chamber at the bottom of the facility.
Demonic Dummy: Literally. The dummy (originally in the room named after it, but later found in Cold Storage) is absolutely, positively evil. It turned to look at them when it was tied to the post, and if that's not creepy enough, later its nature was absolutely confirmed when it grew tentacles on the ceiling of Cold Storage, Ace's room. It was excavated from below the station in the first place. Considering the stuff apparently imprisoned down there, that alone comfirms its evil nature.
Psychic Powers: It is implied that The Dummy, itself, had a sort of special psychic-control over Ace, influencing his actions and causing him to take it from its original place to Ace's hideaway in cold storage.
Running Gag: He is disappointed in us all.note (Because of the position of the water cooler's taps, many players have referenced it as an appearance of a vexed expression. It has since become a sort of running joke by the Ruby Quest players and have referenced the fact many times during later parts of the game, often as a sort of Animal Reaction Shot)
I Want Them Alive: It is never made clear just why Ace is ordered to resurrect everyone just to have them destroy everyone, but the dark entity seems to need them for some reason other than just For the Evulz.
You Cannot Grasp the True Form: In theory, Ruby quite possibly could have in the cold-storage room if her third eye wasn't hurting so bad (of course, trying to use her super-power to view such an other-worldly force directly would probably leave her in a permanent state of insanity)
And what became of cat and hare? Did they break free to purer air? To guess their fate we shouldn't dare Perhaps their tale closed well...