Main characters: The Mario Brothers | Allies (The Princesses) | The Koopa Kingdom (Bowser — Bowser Jr. — The Koopalings — Other High-Ranking Subordinates — Bosses) | Assorted Nasties
RPG characters: Super Mario RPG | Paper Mario (64 — The Thousand-Year Door — Super — Sticker Star — Color Splash — The Origami King) | Mario & Luigi (Minion Quest: The Search for Bowser)
Spinoff series: Yoshi's Island | Luigi's Mansion | Mario Golf | Mario Tennis | Super Mario Maker | Donkey Kong series (Kongs) | Wario series (Wario and Waluigi)
Crossovers: Punch-Out!! | Super Smash Bros. | Mario and Sonic at the Olympic Games | Skylanders | Mario + Rabbids Kingdom Battle | Mario + Rabbids Sparks of Hope
Other media: The Great Mission To Save Princess Peach | Super Mario Bros. (DiC) | Super Mario Bros. (1993) | The Super Mario Bros. Movie
Mario
Mario
Voiced by: Tōru Furuya
- I Just Want My Beloved to Be Happy: After learning that Peach is already engaged to Prince Haru, he gives them their blessings and heads back home.
- The Snack Is More Interesting: Deconstructed during the finale. After he had already eaten the Mushroom and the Flower power-ups and only needed to eat the Star to defeat Koopa, he got distracted by a bowl of rice with Mario Ramen Sprinkles appearing out of nowhere. While he munched on the treat, Koopa didn't waste any time and attacked him, causing the Star to fall down a crack in the ground. If it hadn't been for Luigi finding it (by complete accident) and bringing it to his brother, Mario would have lost then and there.
Luigi
Luigi
Voiced by: Yu Mizushima
Mario's younger brother.- Adaptation Dye-Job: Since the anime was made during a time when Luigi really was just "Green Mario", it's unsurprising that his appearance wouldn't be as it's recognized today. That being said, it even differed from Luigi's designs at the time, such as his green and white in-game sprite. Just about the only sticking aspect of Luigi's design here would be his height compared to his older brother.
- Adaptation Personality Change: Luigi in this movie can best be described as a skinny Wario instead of the Lovable Coward he's known as nowadays. It's unsurprising though, given how early in the Mario franchise's history this film was created. At the time Luigi was just a green Mario, with the earliest known instances of his cowardly personality being The Super Mario Bros. Super Show! (which didn't come out until 1989) and a commercial for Mario Bros. from 1983.
- Big Damn Heroes: After Mario loses the Star and is about to be killed by Koopa, Luigi shows up and gives him the last power-up he needs to win.
- Greed: Luigi's defining character trait in this adaptation.
- Nominal Hero: Does Luigi join the quest to help his brother, save the princess and/or aid the troubled people of the Mushroom Kingdom? No, he's just after the riches he had been promised by the old wizard. And the times where he does help Mario are generally either the product of dumb luck or mutually beneficial self-preservation.
- You Don't Look Like You: Luigi isn't wearing a single piece of his iconic green, instead having a blue hat and overalls and a yellow shirt. This, rather fittingly, makes him look a lot like Wario. His moustache is also styled like Mario's instead of having the smoother trim.
Princess Peach
Princess Peach
Voiced by: Mami Yamase
The princess of the Mushroom Kingdom, who had been kidnapped by King Koopa so he could try and marry her.- Guile Hero: She tried to escape Koopa's clutches by tricking him into transforming into a teddy bear and then locking him into a small chest. Unfortunately for her, it doesn't work.
Kibidango (Unmarked Spoilers)
Kibidango/Prince Haru
Voiced by: Masami Kikuchi
A talking dog travelling with the Mario Bros. It turned out he was actually Prince Haru of the Flower Kingdom, whom Koopa had turned into a dog because he owned the other brooch, signifying that he is destined to be with Peach.
- Canon Foreigner: Prince Haru has never appeared in any of the Mario video games. Even when the Flower Kingdom had been made into the setting of Super Mario Bros. Wonder, he wasn't in the game, instead being replaced by Prince Florian.
- Early-Installment Weirdness: Considering he appeared in a special where the Mario franchise was very young, his romantic relationship with Princess Peach would feel a tad weird to modern audiences.
- Forced Transformation: Kibidango was originally the handsome Prince Haru before Koopa cursed him into his dog form.
- Walking Spoiler: It's impossible to discuss Kibidango without getting into him actually being Prince Haru of the Flower Kingdom or being engaged to Princess Peach.
King Koopa
King Koopa
Voiced by: Akiko Wada
The king of the turtle clan who had kidnapped Princess Peach, wanting her to become his bride. The Goombas
The Goombas
Voiced by: Hiroku Maruyama & Kazue Komiya
Two Goombas working for King Koopa, who keep trying to hinder the Mario Bros on their quest.- Nice Job Fixing It, Villain: Their plans to stop the heroes in their tracks usually end up having the opposite effect. First they led Luigi to the patch of Mushrooms, which allowed Mario to defend himself from the baby Paratroopas and find the Super Mushroom. Next, they recommended the bros go to Lakitu's garden of Piranha Plants, which is where they needed to go anyway to find the Fire Flower. Thirdly, they set up a trail of gold coins leading the heroes into a trap, resulting in Luigi finding the Super Star by accident whilst digging an escape tunnel.