Hotel Rwanda: "Cut the tall trees! Cut the tall trees now!" Also doubles as a Real Life wham line since it also marked the beginning of the 1994 Rwandan genocide and the deaths of over one million civilians.
Kill Bill: "Is she aware... that her daughter is still alive?"
One Flew Over The Cuckoo's Nest: Mac passes a piece of chewing gum to (supposedly) deaf/mute inmate Chief and Chief says "Thank you." This is the reverse of what happens in the book, as the reader knows this secret from the beginning and, we find out, so does Mac. See Literature for the corresponding Wham Line.
"You have your mother's eyes, her hair. I was told you died with her." Classic Wham Line example: you don't expect it, it's a major reveal (Nathan's been dressing up his daughter like her dead mom, wig and all, and told his friend she's dead), and it comes in the middle of a song where it's practically glossed over in favor of a much less shocking reveal ("I'm your... godmom").
Even bigger example: "He's been poisoning your medicine!"
The Conspirator: "There should only be three," referring to the number of nooses on the gallows.
Blade Runner provides an interesting example of how one line can affect a whole scene. When Roy Batty finally meets his maker, Tyrell, he wants more life. There are several cuts of the film floating around. In one cut, he says "I want more life, fucker". In the other, "I want more life, father". The entire nature of the scene is completely different between the two cuts from just that one change.
From the movie Explorers: "Ehhhh, what's up, Doc?"
Red Eye: "Just keeping the focus on you and your father."
For laughs: Mel Brooks' 1976 Silent Movie was just that, with all dialogue on title cards which gets hilariously subverted in the scene where they ask mime Marcel Marceau if he would appear in their film. In the only audible dialogue in the whole movie, we hear Marcel—whose work is exclusively silent—say "Non!"
Algernon, assistant to the scientist Foot, from The Beatles movie Help!: "It's more than my job's worth to stop him when he's out like this. He's out to rule the world, if he can get a government grant." Following the revelation that the Beatles made a big mistake getting help from Foot, he saw what the ring Ringo wore could do: "Fantastic! With a ring like that I could—dare I say it—rule the world."
The line that founded the entire Marvel Cinematic Universe and changed comic book movies forever, "You think you're the only superhero in the world? Mr. Stark, you've become part of a bigger universe. You just don't know it yet. [I'm] Nick Fury, Director of SHIELD. I'm here to talk to you about the Avenger Initiative."
"I am Iron Man." It's not the line itself, but the fact that Tony Stark delivered it in front of a live audience.
Battle: Los Angeles has one for the Marines while they are getting briefed on the "meteor shower." "These objects are not falling at terminal velocity. They are slowing down."
Primer: On the second iteration of the bench scene, "I hope you're not implying that any day is unimportant at Cortex Semi." The words themselves aren't important; what's important is that Aaron says that line even though Abe, exhausted, had failed to say the line it had been a reply to on the first iteration, revealing that Aaron had used a fail-safe and was repeating lines from memory, too.
"You will be rather attractive, one day, not at all beautiful, you understand, but you have a certain prettiness, different than my other heads...I believe I'll lock you in the tower for a few years, until your head is ready. And then I'll take it."
"My Ruby Slippers. They just... fell out of the sky one day- you were so anxious to get home. They're very powerful: they made it possible for me to conquer the Emerald City! Thank you.
Witness for the Prosecution has two: "You didn't do it alone," followed shortly by "I knew he was guilty!"
Plays with this with the repeated line, "It's called a changeover. The movie goes on, and nobody in the audience has any idea." While this line appears earlier in the movie in reference to a projectionist inserting subliminal porn into movies, it's repeated by the narrator later in a fourth-wall breaking manner at a critical moment that the protagonist (and we, the audience) are starting to realise that certain things we'd taken for granted have actually all been delusions suffered by our protagonist and that we therefore have to go back and re-interpret the film so-far.
"Is that a pretty accurate description of our relationship, Tyler?"
In Tim Burton's Batman: Not apparent to the audience at first, the Joker's favorite Pre-Mortem One-Liner serves as this to Bruce. They were the same words the mugger said to young Bruce after murdering his parents.
Joker: Ever dance with the devil in the pale moonlight?
Bane: Let's not stand on ceremony here... Mister Wayne.
Bane: I will show you where I have made my home, then I will break you. [hits a detonator, causing a Tumbler to fall through the ceiling] Your precious armory! Gratefully accepted! We will need it. [Mercenaries raid Applied Sciences]
And later in the film,
Prisoner: The child of Ra's al Ghul.
And soon after,
Ra's al Ghul: Did you not think I would return, Bruce? I told you I was immortal.
Before the climax:
Batman: [about the Pit] You think you're the only one who could learn the strength to escape it? Where's the trigger?
Bane:But I never escaped.
In the climax:
Talia: But he's not the child. And though I'm not ordinary, I'm a citizen.
Plus a truly awesome one in the denouement, to Detective John Blake:
Clerk: You should use your full name. I like that name: Robin.
Bill Biscane: What? Did you think I went though all this trouble just because Dad liked you best?
Sherlock Holmes: A Game of Shadows: "Come now. You really think you're the only one who can play this game?" This serves as the audience's warning that Holmes' ability to plan his strategy before the fight won't help him this time.
Saw: After the whole situation is resolved, Leigh Wanell's character finds a tape recorder, left by Zepp, and plays it. "Hello Zepp", says Jigsaw, revealing Zepp was just another pawn in the killer's game. One of the few wham lines ever to be featured as a track title on a soundtrack.
"[Mr. Boddy's] secret? Oh, haven't you guessed? He's the one who's blackmailing you."
Two after the climax of Karate Warriors, both from Chico (Shinichi Chiba). The first, after catching the kid he'd protected for much of the film tossing expensive heroin in the ocean (under the mistaken assumption that they were the ashes of his deceased father), is "You stupid kid, these weren't his ashes!" Then, just over one minute later, Chico proceeds to confess to him, "The way your father died... I want you to know the truth: I killed him."
Sleepaway Camp: Angela. Such a lovely name. Why I believe it means 'angel'! Why, yes, I'm sure it does! I know you're going to like that name, won't you Peter?
At World's End: During the meeting of the Brethren Court, the pirate captains vote for who should be the Pirate King. As usual, each captain votes for himself (or herself, in the case of Elizabeth and Mistress Ching). Then Captain Jack Sparrow casually says "Elizabeth Swann", giving Elizabeth two votes, where everyone else has only one, making her the new Pirate King.
"The wind's with us." Said twice, by opposing leaders. Cue the Maelstrom.
A rare example of a closing Wham Line: "So tell me, what's become of my ship?"
"The Dutchman must have a captain."
Elizabeth: (after kissing Jack) "It's you (the Kraken) wants, don't you see?" She then proceeds to chain him to ship so he can't run away.
From Curse Of The Black Pearl: "You'd best start believin' in ghost stories, Miss Turner. Yer in one!
In Mortal Thoughts, Demi Moore is being interrogated by police about the murder of her best friend's husband. She is asked about the time of an event, and says "when they found my husband's body," revealing for the first time that two people are dead.
The Prestige has two: "A brother? A twin?" and the following exchange, though, like the Advent Children example above, the Wham factor is due to who says the line.
Borden: You must be -
Angier: Lord Caldlow. Yes, I am. I always have been.
The first is when Chekhov and his team are examining a ship's wreckage on the planet under consideration as a test site for the Genesis Device, and he comes across a belt buckle with the name "Botany Bay" written on it
*confused, and slightly worried* "...Botany Bay?" *a look of horrified realization comes across his face* "Botany Bay! Oh no...!" *full blown panic* "We've got to get out of here now!"
This is followed shortly, by Khan confronting Chekhov, and explaining the dire situation he and his men face, and features two by Khan himself:
Khan: "Admiral Kirk... sent seventy of us into exile on this barren sand heap with only the contents of these cargo bays to sustain us."
Chekhov: "You lie! On Ceti Alpha V, there was life! A fighting chance-"
(the screen flashes to Spock's chair, which is empty)
Bones: "...Better hurry."
In Star Trek IV: The Voyage Home after the trial the crew wonder what ship they'll get to replace the destroyed Enterprise, and reason that it will probably be Excelsior. The shuttle heads for the Excelsior .... then as we hear the familiar fanfair, goes around it to reveal the Enterprise-A.
Kirk: "My friends.....we've come home."
In the 2009 movie, before Vulcan gets consumed by a Romulan-made black hole.
Chekov: Captain, the gravitational sensors are off the scale. If my calculations are correct, they're creating a single energy...that will... consume the planet.
Data: (reading sensors) Population: approximately 9 billion... all Borg.
After Dr. Miranda Grey in Gothika speculates about the psychological profile of a culprit trying to cover his tracks, this line by Doug's accomplice leaves zero doubt about the culprit's true nature and that Miranda's life is in danger.
Sherrif Ryan: Well you're right. I do fit the profile. Long-time friend of Doug's, access to the prison. I never did get a kick out of torturing animals, though.
Another from Dream Warriors is when the heretofore heroine starts sing-songing the Freddy poem, and when she stumbles on the last line, Nancy, the heroine of the first movie, makes her entrance finishing the rhyme from the doorway.
John Doe: I know why you let me go. Freddy: Oh. Do you think I'm your daddy? Mm-mm! Wrong! John Doe: But you let me live! Freddy: Only long enough for you to bring me back my daughter!
In All About Eve, the audience is initially led to sympathise with Eve and feel sorry for her. Even when she seems to have betrayed Margo's trust, her apology seems sincere. That is, until Karen asks her if there's anything she can do...
Eve: "There is something you can do. Something most important."
Planes Trains And Automobiles: Neil Page has finally reached Chicago, so he says his goodbyes to Del Griffith before taking the train home. But during the train ride, Neil remembers something about Del that seems off, so he heads back to the station and finds Del still sitting there.
Neil: Del, what are you still doing here? Why aren't you going home?
Del:I don't have a home. Marie's been dead for eight years.
Cary Grant's character pursues one of the villains across the balconies and finds him discussing his plans with his two co-conspirators, who are telling him what he just did was a "dumb move". Grant pushes his way into the room and says:
"Yes, it was a dumb move, Herman! What is the matter with you???"
By the end of the movie, Reggie's convinced that Carson Dyle is dead, her friend Peter is a murderer, and Mr. Bartholomew is the only man she can trust. With this in mind, she's running away from Peter, towards Mr. Bartholomew, when Peter suddenly cries: