Matt Key (Luigi): "People wanna watch There Will Be Brawl because it's the entire Nintendo universe fighting. I mean, is way beyond the Mario Brothers, I mean, by end of the series we will have like 50 different characters that have made small little cameos parts in there, and that's..."
Paul Duraso (Mario): "That's what you always wanted as a kid. To play Mario and Zelda."
Mario's younger brother, a good man going through bad times, doing what he got to do. Secretly a drug dealer working with his not-friend Wario and some other not-so-noble activities, but he will still do what is right when it's needed.
Gentle Giant: Somewhat. He is not a gentle as other giants, and he isn't that gigantic, but he IS kinder than most of the characters (the exceptions being Red and Peach), and he IS the second tallest characters in the show, shorter than only Ganondorf, so...
The Stoic: Not that much, but there's certainly some degree of it.
Take Me Instead: In episode 6, when Red is captured and about to be interrogated, Luigi cannot bear the thought of letting it happen to someone as innocent as Red and shouts at Sheik to take him instead. It works.
Taking You with Me: He attempted this at the end with Kirby. Apparently, he managed to take away Lucas and Ness, but both he and Kirby survived
It's-a me, Mario! But not the same anymore. Our well known hero here has fallen into infamy and apparent depression. No longer a plumber, failed as a doctor, he nowadays resorts to drinking to drown his problems, but when Peach is kidnapped again, after falling at the bottom for a while, he got back up like never before, determined to rescue her.
Anti-Hero: He'll fight for the Mushroom Kingdom, but is very prone to violence and anger.
Drowning My Sorrows: He spends most of the series as an alcoholic, but in Episode 8, upon seeing Peach's doll when he was about to take a drink, he realizes what he's doing and puts the bottle down, earning another Crowning Moment Of Awesome.
"One child. No matter how far we fall, we are never past redemption as long as we can hear the laughter of one child."
Still the princess of the Mushroom Kingdom, Peach is having a hard time dealing with the newly corrupt city, while her relationship with Mario (and Luigi) is attempted to keep secret, yet it's causing her troubles. She's kidnapped by Ganon to clear the throne for his puppet, Zelda, and is executed by the butchers.
Paper-Thin Disguise: When she's visiting Mario and trying not to attract attention, her disguise consists of dark glasses and a hoodie... over her usual flowing pink gown, with her crown still on her head. The trope is subverted in that Wario does see right through her disguise.
"Lucky for you, girls on my line of work have all sorts of contacts."
A popular stripper who uses her job to get plenty of information. Luigi recurs to her when seeking leads in his search for the princess, and from there onward she's been tagging along in his fight to save the kingdom. In the end, Samus dons her power suit one last time and participates in the final confrontation with Ganon and the butchers. She survives, but loses an eye.
Bi the Way: She's had liaisons with Luigi in the past, and is also shown dancing with Barbara the Bat in Tingle's bar. Though the latter could easily be interpreted as a one-off thing, they're also shown holding hands and comforting each other at Red's funeral.
A young Pokemon Trainer of an innocent and generous personality. He's possibly the only untarnished good guy of the entire cast. After he dares to stand up against Ganon, Ganon has Wario deliver a bomb that kills him. His death and subsequent funeral are arguably the biggest Tear Jerkers in the entire series, and spur Luigi and his allies on in their final assault on Ganon.
Mons: Of course, if he has to do something for a fight, he'll do it with his Pokemon.
Morality Pet: Somewhat to Luigi; his death is what makes Luigi finally snap and attack Wario.
Red Herring: He was conspicuously hiding Jigglypuff from Luigi and his friends in the earlier episodes, and many viewers were suspicious enough of that to have either him or Jigglypuff pegged as the butcher. He gives his (completely innocent) explanation as he's dying, with his Last Words.
Red: Luigi... I caught Jigglypuff! I didn't want you to know until I finished training her. She'll sing for you....
Wide-Eyed Idealist / The Pollyanna: At first, his sunny disposition is strong enough to make him overlook his blatantly bad surroundings, such as insisting that the Pokemon fights, which everyone else treats as bloodsports, are just them "playing a little rough sometimes" and, in episode six, acting completely oblivious to the seriousness of the situation when he's captured by Sheik and about to be taken off to be brutally interrogated. However, he does have his occasional moments where he gains awareness of the situation while impressively not losing his purity or innocence, such as in episode nine, when he is the first to intervene in the standoff between Ganondorf and Captain Falcon. Goes into pure Crowning Moment Of Awesome territory as he is visibly terrified by Ganondorf, yet steps forward anyway, telling him with a shaky voice to "Get out of our Kingdom!"
Star of Tingle's Gay Bar and Captain Falcon's personal bitch. As for himself, Pit is mostly innocent, yet not really heroic. He gets involved in the problems of Mario and Luigi, but remains mostly neutral about the situation. He has a change of heart at the end and convinces Captain Falcon to join the final fight against Ganon. He survives, but not before Ness and Lucas rip off his wings.
Took a Level in Badass: He was a True Neutral and Non-Action Guy party during most of the series, but at the end he He has a change of heart at the end and convinces Captain Falcon to join the final fight against Ganon and takes part in the final battle
Winged Humanoid: As is to be expected of an angel. They get severed during the final battle, however.
"Come back sometime. Maybe... show me your moves."
The one in charge of Tingle's Gay Bar when the boss is out. Like his little boyfriend, Pit, he prefers to stay away from the conflicts arising around the city, but won't doubt to raise his legendary fist in order to rescue Pit when necessary. He skips town instead of helping Luigi take on Ganon, but Pit convinces him to turn back. He survives the final battle, and Luigi's courage reminds him of how to be a hero again.
Big Damn Heroes: His indisputable Crowning Moment of Awesome. And he gets another one near the end.
Domestic Abuse: He angrily smacks Pit in the face at one point. But he won't tolerate anyone else harming him.
Chief of the Mushroom Kingdom police department, and quite aware of how cynical he has become. He's willing to do what he has to in order to employ the law and bring down the mafia groups, regardless of what it takes. He comes to his senses and realizes he's being played by Zelda and Ganon, only for Zelda to stab him with the Master Sword. He recovers in the nick of time with a Maxim Tomato and makes his last heroic stand against Ganon, but fails—the sorcerer kills him.
Children Are Innocent: Realizing that this trope used to apply to him when he was Young Link/Toon Link, he tries to pull a Heel-Face Turn... only to get stabbed by Zelda for it.
Former princess of Hyrule, a nation that has fallen and been absorbed into Mushroom Kingdom. She works for the police, either as an Office Lady as Zelda, or as a secret agent as Sheik. However, it seems that her ambitions go too far... It's revealed that, having lost her faith in Link, she's turned to Ganon to restore her to power. At the last minute she realizes the error of her ways—and is killed by Ganon when she tries, futilely, to save Link from his attack.
A couple (yes, in the romantic sense too) of officers working under Link's command at the police department. They pass out as mostly formal, but aren't really too nice. A flashback implies Marth's partner was Roy, before his death. Ike is mortally wounded in an alley brawl, prompting Marth to rethink his priorities. He joins Mario's army, and after Link's death becomes the new police chief, presumably doing a better job at it than Link did.
Adaptation Dye-Job: Ike is given black hair, likely to differentiate him from Marth, though it's shoulder length and straight rather than spiky.
Red Oni, Blue Oni: Surprisingly, as opposed to their personalities in game, Marth's Red to Ike's Blue. Though they're mostly quiet, Marth comes across as much more hateful and merciless, openly mocking how much the Mario Brothers have fallen and even spitting at disgust at Luigi, who only wanted to help. Of course by the last episode, Marth becomes much more noble and responsible. Though just as arrogant, Ike seems to be less cruel and more dutiful. He also seems to be more sympathetic (despite his infamous quote), as he actually saves Luigi from getting shot by Falco, and actually reluctantly chooses to let Luigi go, prompting Luigi to come back and attempt to save him from Snake.
OC Stand In: Like most of the characters, Isaac acts nothing like he does canonically, going from a noble silent warrior to a cruel and sadistic police officer. Especially since he is a minor character, and does not gain the justification behind their newer natures like more major characters (like Link and Bowser), with whom we get to learn what caused them to change so.
A... whatever he is, working for the Police Department for only one reason: Watch over Kirby. When Kirby escapes during a jailbreak, it seems Meta Knight will finally have his revenge on the cannibal... but Kirby kills him instead.
Sells drugs and engages in other unethical activities. Wario was working under Mewtwo's command until the mafia don was killed. Wario is potentially the most unlikable character in the series for how much of a jerk he is, and how willing he seems to be to backstab anyone as long as it benefits him. Working on Ganon and Zelda's orders, he sends the mail bomb that kills Red. Red's girlfriend, Leaf, tracks him down and kills him as revenge.Played by Kyle Hebert. Yes, really.
Played by Jeff Lewis (you know, from The Guild!)Wario's mentally disabled brother. Wario doesn't treat Wa very well, keeping him locked in a pen most of the time, but the fact that he looks after Wa at all is his sole redeeming quality. Wario heartlessly uses Waluigi to deliver the bomb that kills Red.
Children Are Innocent: Well, mentally he's a child, anyway. Subverted when Wario uses him to kill Red, but even then it's implied Waluigi doesn't realize that what he's doing is wrong.
They're working under Wario, and one of the most prominent examples of how far the Darker and Edgier setting has gone. They never speak, but you really wouldn't like to share a word with them. They're subdued by Marth in the final battle and presumably brought to justice.
Chaotic Stupid: Is it a really a good idea to smack your brother on the head with your hammer in the middle of a battle and knock him out? No, but Nana did it anyway. Because she's evil and wanted to take Marth on mano-a-mano (AKA, Marth was duel wielding his and Ike's swords, so she wanted a second weapon).
Used to be Mario's pet until he had to sell him and two other Yoshis to Wario for financial problem, after failing his medical practice. Yoshi seems to have grown aggressive and feral under Wario's watch. Still, when confronted by Mario, as he puts it, Yoshi remembers who the real Alpha Dog is. Killed by the Ice Climbers—at Zelda's command—to send a warning to Mario. (It doesn't work.)
Forget everything you ever knew about the cute and lovable Nintendo character here, because this Kirby is a walking Homage to Hannibal "The Cannibal" Lecter. He was the monster who raised terror in the Mushroom Kingdom six years before the beginning of the series, until Mario and Luigi managed to stop and imprison him. During a jailbreak, Kirby kills Meta Knight and escapes. It turns out that he was the one who inspired Ness and Lucas to become the butchers. He kills Ganon and seems poised to begin a new reign of terror, but Luigi sets the building ablaze, apparently killing the cannibal once and for all... until Kirby resurfaces at the end, letting Luigi know he's alive and well elsewhere.
For the Evulz: His typical motivation for killing his victims. This in fact worked to his advantage during his reign, as it made it extremely difficult for the police to capture due to the fact he murdered indiscriminately.
Mind Screw: The entire series was just him doing this to Luigi, using everyone else as tools
To the fans: he tricks Luigi into dropping hints that Meta Knight or someone similar might be the butcher. He outright implies it when he says that Meta Knight has come to understand the mind of a killer quite well. He then shows up with Meta Knights's mask, sword and voice in the finale, as he reveals the true butcher(s) was just his pawn.
"The last thing this city needs is more bloodshed."
One of the four mafia dons, and the most likable around the kingdom, having gained popularity among both the poor and the rich. He runs a whorehouse and is somewhat unfriendly when dealing with people, but despite his position, he wishes for the arising crisis to end. Killed by the butchers not long after his first and only scene.
"Your kingdom will know its new ruler. And if it doesn't, it will burn."
Another of the mafia mobs, still resenting the Old Shame that was the fall of Hyrule, mostly due to the fact the kingdom fell due to a bad economy rather than his minions and magic. He plans to take over Mushroom Kingdom, manipulating everyone he can in order to get his way. He kidnaps Peach and tries to set Zelda in her place as his puppet monarch, using Mr. Game & Watch as his army and Ness and Lucas to eliminate his rivals, but he loses control of the two butchers to Kirby, who kills him.
Even Evil Has Standards: Sort of. Though he's certainly evil, Ganondorf seems to actively look down on Ness and Lucas because they're doing things For the Evulz. That, and he really got fired up talking about Hyrule as a kingdom of chivalry and heroism where men lived and died by the sword. Like most people in the series Ganondorf preferred the Glory Days.
One of the four mafia dons and Mario's Arch-Enemy, but as he says to Luigi, days like these are no longer fit for old fashion brawlers like Mario and him, so he has grown apathetic, losing interest in villainy. Now he only cares about enforcing order among the Koopas. Despite being the main suspect for having kidnapped Peach, it turned out he didn't. Bowser was poisoned by Ganon and Mr. Game & Watch in their bid to conquer the Mushroom Kingdom, but chooses to take out as many of the G&Ws as possible with a suicide bomb.
One of the four mob bosses who run the Mushroom City's streets—until his untimely death. The police found his internal organs stuffed into a Pokeball. Ouch. Mewtwo was apparently a ruthless mafia don who nonetheless cared for his fellow Pokemon. In the final episode, we learn that in response to Kirby's rampage, Mewtwo tried to create a psychic army by experimenting on orphaned children. Two of his creations, Ness and Lucas, fell under Ganon's thrall.
Go Mad from the Revelation: Implied. Kirby is said to have "whispered" to him, and and afterwards he tried to raise the children as part of a "psychic army". It's possible that Kirby used him to set the Butcher event in play in the event that he would be arrested. If so, he's also an...
They first appeared kidnapping Luigi for Ganondorf, and later as bouncers in Dedede's bar covering the whorehouse. Mario nearly kills Donkey Kong in a rage when he sees the ape with his former girlfriend, Pauline. Diddy tries to kill Mario in the final brawl, but ultimately it behind him.
Jerk Ass: They don't seem to be much beyond this here.
The Rival: Donkey Kong still holds a harsh relationship with Mario even today.
"Wave of future! Everyone having one!" (Regarding Landmasters)
Three bandits wandering around the dark alleys. They easily scream trouble from a mile away. Wolf is killed by Marth in an alley fight, while Falco is sacrificed by Olimar's cult. This prompts Fox to rethink his life and join Mario's crusade. He's killed defending Marth, Ike's boyfriend, from Mr. Game & Watch.
Both Canon Immigrants appear depicted as homeless and crippled, surviving however they can around dark alleys, and seem to be look after one another in order to survive any hardship that comes their way. They unfortunately were cut from the final episode due to time constraints, but were originally going to appear.
Back Stab: Snake attempted this on Ike, in a possible reference to the fact that one of the earliest screenshots of the game showed Snake grabbing Ike by behind.
Known as "End of Days", and damn insane. His actions and lines are the creepiest, his motives incomprehensible. If he's present, expect something nasty to happen. He's recruited by Mr. Game & Watch—his so-called demons—to join Ganon's plot to overthrow the Mushroom Kingdom. In the end, G&W kills him in a frenzy during the final brawl.
Cryptic Conversation: When Luigi meets him, Olimar babbles on at him with seemingly meaningless nonsense and doomsday cliches. Although in retrospect, it turns out that he was providing foreshadowing about the Game & Watches.
Usually black (but also seen of other colors) 2-dimensional beings seen around the Mushroom Kingdom, usually in minor roles. It's unclear if they're separate beings or if they share a Hive Mind. They were the first inhabitants of the Mushroom Kingdom and secretly harbored a hatred for its current citizens. They make a deal with Ganon to help them reclaim the kingdom, acting as his army, but are killed by Mario in the final brawl.
Two little kids seen around the alley at one point, serving as Luigi's inspiration for going on fighting. Their role is a little expanded for some of the scenes of the finale, but they are pretty much background characters. Just kidding. Later they revealed to actually be the butchers, endowed with Psychic Powers from Mewtwo's experiments and taken in by Ganondorf—and, later, Kirby. They are able to kill by using their powers to rip the organs right out of their victim's bodies (also using a snake and baseball bat for more damage) and you don't even want to know what they do to the bodies afterward. It is implied that Kirby eats them (or at least Ness) after the final battle.
Awesome McCoolname: Not in-universe, but Ness's actor is named Tibet Axelrod, how awesome is he gonna be.
Batter Up: Ness carries his trademark baseball bat around.
Tom Nook from Animal Crossing, the dogs from Nintendogs, Jeff from Earthbound, Dr. Wright from Sim City, and the Koopa Kids from Mario are shown/mentioned in the Mushroom Kingdom News extras hosted by Lakitu.