- When Black Widow is working on the '70s-era computer, she says, "Shall we play a game?" She begins to explain the reference but Rogers has already gotten a chance to see WarGames (which could also a Call-Back to a scene in The Avengers where he also knows that reference).
- Zola's avatar resembles Dr. Blight's snarky, bespectacled AI sidekick MAL from Captain Planet and the Planeteers crossed with some Matrix Raining Code.
- Steve's notebook, in which he's written a list of things from the past 70 years he needs to catch-up on, is littered with these. Exactly what's on the list varies from country to country to fit in with pop culture trends in that area:
- The US version includes I Love Lucy, Star Wars, Star Trek, Nirvana, and Rocky.
- The UK version includes The Beatles, Sean Connery and Sherlock.
- The German version includes the rise and fall of the Berlin wall.
- The South Korean version has Oldboy (2003) and famous Korean soccer player Park Ji-Sung.
- The Italian version includes Italian singer Vasco Rossi, Ferrari's victories at F1 Grand Prix and Roberto Benigni.
- The French version mentions the 1998 World Cup, Louis de Funès, stand-up comedian Coluche, The Fifth Element, and Daft Punk.
- The Australian version mentions Steve Irwin, Skippy the Bush Kangaroo and Tim Tams.
- The Russian version mentions Yuri Gagarin, Vladimir Vysotsky, and Moscow Does Not Believe in Tears.
- Here are some screencaps.
- A subtle Pulp Fiction one (and Actor Allusion for Samuel L. Jackson): (Tarantino's version of) "Ezekiel 25:17" is quoted on Nick Fury's "grave". "If anybody needs me, they can find me right here" at the lonely grave of Nicholas Fury.
- Robert Redford gets a subtle one with his character, whose ultimate goal is to create a Utopian prison, wherein the prisoners believe they are completely free—a shout-out to his biographic film Brubaker.
- Gary Sinise is the voice-guide of the Captain America museum exhibit.
- Star Wars shout-outs:
- The gaunt commander of the Insight Helicarrier tells the weapons officer, "You may fire when ready."
- The shot of Pierce looking out at the Helicarriers as they fire on Falcon is a shout-out to the Emperor observing the rebel fleet's attack in Return of the Jedi.
- Fury's "torch" looks a bit like a lightsaber, and it's a lot more effective cutting through solid concrete than Qui-Gon and Obi-Wan's attempt to get through an equally thick metal door.
- This movie seems to have taken a few notes from The Fugitive, seeing as Cap goes on the run from S.H.I.E.L.D.
- Pierce tells Cap that his father "fought for the 101st", which is a nod to Band of Brothers.
- Real-life hacker/whistle-blower Edward Snowden appears in cyber-Zola's montage; in the documentary Citizenfour he uses the HYDRA metaphor ("cut one head off and two more will take its place"), referring to the other people who have his information in case he's captured.
- A S.H.I.E.L.D. flight controller offhandedly says "Negative, the pattern is full."
- When Black Widow kicks Jasper Sitwell off the building. Is that Leonidas did on the messenger in the 300 movie but unlike it Falcon managed to save him to get the info they need.
Shout Out / Captain America: The Winter Soldier