Durarara!!: Shinra, after Izaya trolls him by handing his cellphone number to the police.
Izaya: How was it? Gave you quite the adrenaline shot, didn't it? Did you and Celty just grow to love each other even more due to the misattribution of arousal? Shinra: Ah hah hah. Go die chest-first in a clay ice shaver, Izaya.
In The Sandman there's a scene where Lucifer is asked if he would ever return to Hell. He stares for a moment and then says exactly this.
Jareth from Labyrinth pulls this on his goblin Mooks after telling them the "You remind me of the babe" joke.
In Toy Story, Woody does a form of this after Bruce the toy shark's lame impression of him after finding his hat.
Bruce: Look! I'm Woody! Howdy howdy howdy!
Woody: Ah-hah, ah-hah, [yanks his hat off Bruce's head] gimme that.
There's a very sinister example in The Lives of Others, which is set in communist East Germany. A young Stasi agent in training is telling a (quite funny) joke to some of his friends that could be interpreted as being anti-government. A Stasi captain overhears it, and when the joke is finished, he laughs heartily... and then asks for the joke teller's name and rank, presumably so that he can be fired or imprisoned. And then he says he was just kidding, tells an even more anti-government joke, and leaves the stunned jokester be.
In The Clones of Bruce Lee (which is about, you guessed it, Bruce Lee clones), the villain Dr. Ni gives a hearty laugh, then immediately yells "Silence!" at all his henchmen laughing with him.
The first Night at the Museum has the boss do this, after Larry tells him that the mess with the fire hydrant was caused by the (now-inanimate) cavemen. Gervais' character simply ask if he should laugh, laughs silently with exaggerated expressions, then returns to telling him off.
Ferris Bueller's Day Off: Cameron does this in response to Ferris' suggestion that they take the miles off his dad's Ferrari by driving home backwards.
In Scream 1996, when Randy asks Stu if he thinks Sidney would go out with him, Stu laughes and points at him in the most flamboyant way possible before putting on a deadly serious face and saying "No. I don't. At all. No."
In the Spider-Man Trilogy, there's also a scene that has been used by many to emphasis this trope, with J. Jonah Jameson laughing at Peter Parker's claim before asking, "You serious?". Seen here
A Christmas Carol inverts this trope. A reformed Scrooge pretends to direct his anger at his assistant for being late, then abruptly starts laughing and saying "Merry Christmas!"
Used many, many times by Uncle Phil in The Fresh Prince of Bel-Air. The laugh rises up into a high pitched, somewhat creepy register, but he loses the exclamation on "No", and goes straight into thunderous shouting.
For example in one episode, Will and Carlton lose Vivian's bracelet which they pawned for a stock investment that quickly tanked. Phil finds out about this and starts laughing which the boys join in. When asked by Phil what he should do, Will jokingly reply he should "reward them for their honesty... it worked for The Beav" causing Phil to laugh louder.
Firefly: Jayne makes a suggestive comment about Zoe, who laughs and replies cheerfully "I can hurt you."
He does the same thing regarding Inara, who has a similar response.
A slightly more articulate variation: on an episode of M*A*S*H, Col. Potter is trying to bring the meeting to order and Hawkeye makes a lame joke. Potter, laughing, says "Pierce, sometimes you can be very amusing," and then abruptly stops laughing and says "And then there's now."
Mad Men has a hilariously cryptic example when Bert Cooper asks Duck Phillips, who's up for a job with the company, whom he's voting for. Duck good-naturedly answers that if he says Nixon, Cooper will think he's sucking up, and if he says Kennedy, he'll want to reform him, so he'll say... Nixon. "That's nice to say," Cooper responds with a smile — which abruptly disappears... and then abruptly reappears — and then abruptly disappears again. Duck just stands there looking bewildered until he's led away.
Buffy the Vampire Slayer. Giles is none too pleased to learn that Angel, who nearly tortured him to death the last time they met, is still alive. He's even less pleased when Angel shows up at his doorstep.
Angel: I'm sorry to bother you.
Giles: (Stares, then bursts out laughing). Sorry. That's just rather funny, coming from you. (Chuckles) "Sorry to bother me."
Angel: I need your help.
Giles: And the funny keeps on coming.
Angel. When Darla and Drusilla surprise Lilah and Lindsey, the former two laugh evilly at Lindsey. Lilah starts to join in, but they snap at her to shut up.
Hannah Montana when Jackson, for the second time, has horrible luck with his sister's birthday cake and the seagulls come to eat it. His friend makes a joke and starts laughing with Jackson joining in; a minute later he then tells his friend to go home.
Cyrano invokes this trope at Act I Scene V when he considers the possibility that an ugly man could love.
Le Bret(stupefied): How now? What? Can it be...?
Cyrano(laughing bitterly): That I should love?...
(Changing his tone, gravely): I love.
De Guiche plays it straight at Act II Scene VII when he reveals to the multitude that celebrates Cyrano combat against a one hundred men that he was the man who hired them against a poet for a satiric poem:
Red vs. Blue: Washington delivers the Trope title (in full snarking mode) when Doc suggests the Reds are behind a well thought through trap. Made quite creepy by the fact that Washington never laughs otherwise. Its probably for the best.
There's also a very early example with Caboose, of all people.
Tucker: I'm sorry, what? It's kind of hard to hear you over the sound of your constant team-killing. Caboose: Ah ha ha ha ha ah ha yeah ah hah—Don't make me mad.
Counselor: Ain't no such thing as too young! You're just too old!
Black chef: (boisterous laugh)
Snob: Ha ha ha ha — oh, pedophilia!
The Top 11 Cinema Snob Moments of 2011:
Snob: (venomously) Ha Ha Ha I PUNCHED SOMEBODY!note A reference to his crossover with Obscurus Lupa, where he failed to pull a punch for one of the gags.
Phelous once did a unique variation on this, when reviewing the "funny" version of House of the Dead. There was a scene where it suddenly started playing in reverse for no reason. Phelous laughed out loud, heartily; then the video reversed to before he started laughing and he said "Actually that's not very funny at all."
From MMO Grinder's Maple Story review "The official statement from Nexon's FAQ was that being Level 20 in Maple Story meant a lot more than being Level 20 in OTHER MMO's, to which I thought 'Hahahahahahaha...no'"
Ranier: Bart, your little tie makes me smile. Bart: Excuse me, but you don't sound as tough as you do in the movies. Ranier: [threatening] If you don't shut your big yap, I will rip off your face and use it as a napkin. [pause, and then everyone laughs] Ranier: [serious again] Laughing time is over.
Also from "Lisa on Ice":
Homer: OK son, just remember to have fun out there today. And if you lose, I'll kill you! [everyone laughs] Bart: [good-humored] Oh, Dad. Homer: [looks menacingly at Bart] Bart: [cringes]
Also from the episode where Homer becomes trash commissioner, and has to deal with (actually, ignore) the garbage crisis: Homer is about to leave Moe's. When Moe asks him to pay for his drinks, Homer says his campaign catchphrase: "Let someone else do it." Moe laughs, amused. Homer starts to walk out. Moe cocks his shotgun: "Seriously, give me the money."
Futurama: Hermes wants Leela to sign an indemnity waiver in case she is killed on a mission.
Leela: I don't know about your last crew, but I plan on doing as little dying as possible.
Adventure Time has the Earl of Lemongrab, upon finding out that his "attack" was a prank.
Lemongrab: Prank...? For laughs...? Yes, of course... Just a prank... For laughs... AhhhhaaaAAAAHAHAHAHAHA!!!! HAHAHAHAHAHA.HAHAHAHAHAHAHA—twelve years dungeon. All of you, dungeon. 7 YEARS, NO TRIAL.
In Scooby Doo Mystery Incorporated, when Fred is complimented by his father and asks him if he finally accepts what Fred wants to do with his life (solve mysteries using ridiculous traps), he gets this trope in reply.
In Rocko's Modern Life episode Dear John, Rocko hires a hyena contractor to fix his kitchen but the wreckage is so huge and costly that the hyena contractor guy bursts into hysterical laughter over it.
Is the final line of subtitled dialogue in the silent short "Teddy Bear's Picnic," featured in Spike & Mike's Sick & Twisted Film Festival.
At the end of Regular Show episode "Bet to Be Blonde", we get this exchange:
This happened behind the scenes on MythBusters. They were testing whether certain sugary breakfast cereals are in fact less nutritious than the box they come in. The test involved three groups of mice: a control group (A) being fed standard mouse food, a group (B) being fed sugary cereal, and a group being fed cardboard processed into mouse-food-like pellets (group C). All three groups of mice did fine all week. Then everyone left for the weekend. When they returned on Monday, there were no longer three mice in group C. Instead, there was one very fat mouse and two mouse skeletons. When the team showed the footage to Discovery Channel executive types, this was their reaction in a nutshell.