The whole chase scene in general. Props go to the end of the chase where Hater rises up behind Wander with bloodshot eyes and looking ready to strangle him.
After chasing Wander, Lord Hater realizes that he has given Emperor Awesome the top spot on the temple and he is about to be given his wish. So what does he do? He runs toward the temple, bowls through Emperor Awesome's army, throws his giant dinosaur into space and punches him with enough force for him to go through the entire planet. And yet he STILL can't do anything about Wander.
The Celestial Being makes an epic entrance, and then says...
Celestial Being: Hey there. So, uh...I'll be the one granting your wish today, 'kay?
His entire attitude in general, including flash-smiling for Wander's photos while looking utterly stoned the rest of the time.
Lord Hater: Soon, every planet in the universe will be mine! Even pathetic little specs like Banglebrop, all under the control of... LORD HATER!!! *cue lightning*
Wander: The baby needs to know we're helping it, NOT hurting it! It needs some reassurance! It neeeeeeds... Sylvia: Don't tell me— Wander: A HUG! (looks at her with eyelashed Puppy-Dog Eyes) Sylvia: Over. My. Dead.BODY.
Sylvia's reaction to the 'reveals' of each good deed- Specifically the one with the ant army when she's just standing there in stunned silence as a Watchdog punches her (with no effect what so ever, following with her punching the Watchdog off with no problem at all)
The Good Deed
Wander and Sylvia's song rewinding as they walk backwards to help a starving creature.
The ENTIRE courtroom scene.
Wander's numerous good deeds eventually cause a sun to slightly shift. This causes a nearby fortune teller to inform her client that he will not be a strong and powerful leader, and instead is immature, awkward, and emotionally unstable. Guess who she's talking to?
Lord Hater's wants Peepers to bring Wander to his torture chamber.
Lord Hater: Now I'm going to my torture room and I want you to bring Wander immediately. Or else. RRRAAAAGGHHH!!! Got it pal?! Psyche, we're not pals, Hater Out!...Seriously, this is not going to be easy he's a tricky one, Hater Out!
And then he teleports out, and back in one more time just to do the 'I've got my eye on you' gesture.
The ending. After deciding that Captain Tim is too dangerous for him to keep as a pet, Wander says that he still wants to find him a forever home. So, they give him to Lord Hater who of course loves the little monster. But of course this also results in the Watchdogs getting chased/chewed on by Captain Tim.
Adding to that, Lord Hater takes CAPTAIN Tim's title seriously, saying that his pet is now ranked higher than Commander Peepers.
The Bad Guy
The residents of Doomstone revealing they're all a bunch of softies.
Papa Doom literally crying like a baby.
All of Wander's attempts to act like a bad guy. Just, all of them.
Sylvia: *Listening to Wander growl* Is, is that some dinosaur in there?
Wander: Little bit!
Sylvia: Ohhh we're doomed.
The moral of the episode: Don't. Feed. The Troll.
A lot of the goat warriors comebacks come out a bit weak...
Wander shaking his head and rolling his eyes while smirking when he realizes what the others don't when facing the troll.
Wander's breakdown upon seeing the 'Gone Fishing' sign.
"YOU CAN'T BOX ME, BOXIA! I'M GONNA BOX THIS BOX AND THERE'S NOTHIN' BOX YOU BOX BOX BOX!"
During various points in the episode, it looks like the bottom of Wander's hat has a smiley face or a frowny face. It's funny because it seems like the hat has a mind of its own.
Sylvia reaching into the hat to get something to save Wander...and pulling out a "Back in 5 minutes" sign.
The Little Guy
While you have to feel for the guy, the scene where Westley calls up Peepers to come pick him up is pretty funny.
Westley: No, I don't have them, Sir. But that's not the only reason you'd come and pick me up, right? Peepers: What else is there? Westley: Maybe because I'm a good soldier and you like me? Peepers:[uncontrollable laughter]
Peepers' rant after the Watchdogs failed to capture Wander and Sylvia.
Peepers: ARE YOU KIDDING ME?!
Said failure involving going the entire squadron going over a cliff into a pit like a herd of lemmings only makes it funnier.
The whole series of accidents Wander and Sylvia have to save Westley from after he 'captures' them.
Lord Hater, getting out of the ship to get Westley. "This better be worth it, Peepers! I had a brunch today!"
Lord Hater may not be in the episode much, but when he is, it is just comedy gold. The way he yells at the screen while watching the security camera footage like it was an action movie - even eating popcorn while watching - and his breakdown during Westley's funeral (which is also kind of sweet when you think about it) are just comedy gold.
The ending with the yarn planet and the giant cat.
Hater playing a Street Fighter-like game with himself and Wander in it.
Peepers: Seriously? You hired a plant to do MY job? Hater: Well, he can't possibly be worse at it than you, can he?
Sir Brad Starlight is a walking punchline, what with his big ego and no real skill to back it up, his living in delusion, and his being on the receiving end of pretty much all the episode's slapstick for both of those reasons.
Sylvia snapping in and out of her 'bomb' mode for all of two seconds to trip another competitor in the middle of explaining what the bomb mode is.
Wander and Sylvia's VERY short stay at the hotel.
Concierge: Enjoy your stay!
Sylvia: (grins) We will!
Wander: (runs into room for quick second, then runs back to join Sylvia and Concierge in elevator) We DID!
Sylvia: (in disbelief) We WHAT?!!
Sylvia chuckling while expressing disbelief that Wanderof all people is getting competitive with Trudy, especially considering that Sylvia got over her Competition Freak issues a couple episodes prior.
During the travel montage, Sylvia becomes increasing exasperated at Wander's rushing through the various tourist sites.
The fact that during their visit to Shatterton (a town made entirely out of glass), the only thing Wander breaks while rushing the bus is the farewell sign.
After the entire quest to find the all-knowing being whom they can only ask one question- "Is this your sock?"
When the sock that Wander and Sylvia have been going through all the trouble throughout the episode to return to its rightful owner, is finally returned, it's revealed that the sock is actually a hand puppet that acts as a Morality Chain for Destructor. Cue a series of flashbacks and current events that emphasize how ridiculously significant Destructor having the sock as hand puppet is to the entire whole kingdom. For restoring the kingdom to its ridiculously happy glory, Wander and Sylvia are praised as heroes as they leave. In the end, not even Wander and Sylvia understand what exactly just happened.
The song of Wander's, along with the various things that happened during the song, even Sylvia being FORCED to sing along.
Among the various random images behind the door Sylvia tries to use is Jack McBrayer. The guy who voices Wander.
Another one of the random images is The Two Man Gentlemen Band, who provide all the music for the show.
Another one of the random images is a message saying, "Thanks for watching Wander Over Yonder and for taking the time to still frame this! - CM"
The final image before the insurance sales-squid is a black and white photo of Craig McCracken himself.