Ogura Sio/Oda Nobunaga ("Nobunagun")
Sio voiced by: Shiori Muto (Japanese), Jad Saxton (English)
- Cute and Psycho: Sports a killer Slasher Smile when she goes into battle.
- Friendless Background: She spent much of her time in school alone, reading magazines on military weapons, and people thought her weird as a result and didn't really hang out with her.
- Not a Morning Person: Frequently wakes up late.
- Small Girl, Big Gun: The cartridges alone are bigger than her torso.
- Socially Awkward Hero: She's not very good interacting with people, especially those as strange as her fellow E-Gene Holders.
Adam Muirhead/Jack the Ripper
- 11th-Hour Superpower: In the final episode he reveals abilities related to the true identity of Jack the Ripper.
- Swiss Army Weapon: His blade can split apart into a claw of sorts, made of different kinds of blades.
Mahesh Mirza/Mahatma Gandhi
Voiced by: Nobunaga Shimazaki (Japanese), Christopher Bevins (English)
- Mr. Exposition: He explains the bases and the platoons to Sio.
- The Tease: He tends to jokingly flirt with Sio.
Jess Beckham/Isaac Newton
- Bi the Way: She apparently has no problem kissing people of either gender.
- Forceful Kiss: Her way of greeting people is with a French kiss.
- Giant Foot of Stomping: Her weapon seems to be one crossed with an abacus. For extra stomping power, it can apply Selective Gravity to whatever it steps on.
- Fish out of Water: As a Non-Action Guy, he feels out of his element during the "Stone Forest Operation", which result in Jack making fun of him.
- Bratty Half-Pint: She can be rude and haughty towards Sio, since she was the previous newbie, despite the fact she appears to only be in elementary school.
- Only One Name: Is referred to as Lemond by Hunter in episode 13. It's unknown if this is her first or last name.
- Slasher Smile: Like Nobunagun and Jack, she too tends to smile sadistically while in combat.
Ibukigin "Esui" Erdenbileg/Cyx
- My Horse Is a Motorbike: Cyx is based on the Mongolian story "Suho's White Horse" which is why she rides that white vehicle and they call her Suho.
Voiced by: Ayumu Murase (Japanese), Sean Michael Teague (English)
- Loving a Shadow: Develops a crush on Sio before even meeting her and is disappointed by the reality of her. (Though this is mainly because he had a shopped photo of her and was not happy when he found out she wasn't as endowed as the photo implied.)
- Cynical Mentor: He's a bit of a Jerkass when Sio meets him, and to the end, he expresses doubt over whether or not Sio is worth the fuss. However, he recognizes her great potential and seems motivated to train her so she doesn't get butchered in real combat.
- Dirty Old Man: Invoked. He really has no (specified) interest in Sio, but he uses lecherous and perverted tactics to motivate her into completing her training when she starts slacking off.
- Master of Illusion: He has the ability to produce illusory copies of anything he captures within his camera.
- The Mentor: He's put in charge of training Sio immediately after she joins DOGOO.
- I Was Quite a Looker: Flashbacks to her youth reveal she was very beautiful.
- The Stoic: He barely reacts to anything.