Pretty much all of Parasoul's story mode intro. Especially the part where Parasoul instructs one of her soldiers to send soldiers to help her hunt the Skullgirl down...and get Umbrella some ice cream. Said soldier who got the order then calls it "Operation Chocolate Dawn".
Any of the announcer's annoyed reactions to a lame match ending via chip or time over:
Announcer: This racket's flopped. Announcer: Awwwww, c'mon! Announcer: You call that a fight?
Should your opponent actually be dumb (and lucky) enough to let you kill the last two of his characters at the same time onscreen, John "Dangerous" Rockefeller will laugh, and it earns you a trophy.
When knocked out with her head off, Ms. Fortune's head tries to get back up. She only gets far enough to show the audience her incredibly silly face before flopping back down.
On the Meta side of things, some of the voice actresses from the game have a few rounds with each other, and each one of them descends into Crowning Moment of Funny material. The first video alone includes Erin (Parasoul) descending into raving insanity for about 30 seconds and putting twists onto her existing lines, while Sarah (Peacock) raves about shouting things like "I see London, I see France..."
"Look at that, I crowned a princess!"
Pretty much any line Sarah says due to her being a Large Ham.
"TAKE THE SHOT! TAKE THE GODDAMN SHOT!!"
"Where the hell are my followers? Goddamn Egrets!"
At the end they are both reduced to incoherent screaming.
Sarah: Did we just have sex?! Is that what happened here?
The battle between Laura (Valentine) and Sarah (Peacock) is pretty glorious as well. Starting with Sarah shouting "HELLOOOOO NURSE!!", followed by Laura trying to convince Sarah there is candy in her truck, prompting Danielle (Painwheel) to say "Don't go in that truck, I made that mistake and look at me now!" Also "WHAT HONOR?! I'm a fuckingcartoon, you bitch! I have nothing!"
Sarah's Peacock gets away from Laura's Valentine, who was sawing her head off in mid-air:
Sarah: No, no! It's elective, it's elective surgery! Laura: I dunno...I could really help you, uh, look better in that dress... Sarah: You—Have you seen my measurements on the website?! I have no figure, there's nothing you can do for me!
Then, in Round 2, there's this moment after Sarah successfully lands an Argus Agony Blockbuster move:
Sarah: "Oh, there it is! Finally, I countered her! I countered her. Look what I did, Mom! Look what I did! Are you proud of me?! Do you love me now, Mommy?!" (Peacock then gets hit by an EKG Flatliner attack) Laura: "No. No, Mommy does not love you at all."
Speaking of the EKG Flatliner, Sarah often lets out a high-pitched "EEE!" when she gets hit by one.
And then there's every time when Laura accidentally activates the EKG Flatliner in the air and misses, followed almost ALWAYS by "NO NOT IN THE AIR!!!"
Erin (Parasoul) vs Laura (Valentine)'s 1st fight. Anything from near the end of Round 1 and onward is just going to have tears coming out of your eyes, and most of the time it's going to be Erin's innuendo or Large Ham moments. These moments are connected to each other practically in the span of seconds, and it doesn't even end there:
Erin: (after just beating Laura) OHHHHHHHHHHH, YES, THAT FELT GOOD! OH, I AM HEALED! Praise the Lord!
Erin: Oooh, I feel better already! You are good for my health!
Erin: You don't know how to operate your titties the way I know how to operate my titties! My titties are all organized.
Kimlinh: Yes! I nommed you!... Your boobs taste funny. They taste like plastic.
Laura(after winning a round): You might be a Skullgirl, but I'm a Skullwoman!
And now they play the story mode, with some dubbing involved. The hilarity in these cases however stems not only from the voice actresses, but the cameraman, AKA one of the Egrets. Crowning goes to his performance as Dr. Avian, and Fat Albert!Double.
"I'm thirteen; I'm a fucking tween!"
"Dr. Avian! Who did this to you...?" (beat) "WHERE THE FUCK WERE YOU?! Look at me!"
"Wait, what about if it's just the tips touching?"
Sarah: "Then you should probably have a long talk afterwards."
The VAs are at it again during the Breast Cancer donation drive: Every main voice actor for the game except for Danielle attended, and they did more story mode "dubbing". Since Del couldn't come, they got Mike Z to dub Samson... which, of course, he did in his REAL SOVIET DAMAGE voice.
They held another livestreaming event for the Breast Cancer donation drive: The only absent VA was Christine (with Danielle and Sarah making late but grand entrances). Even more story mode "dubbing" ensued. Other highlights include Laura, Cristina, Kimlinh, and Erin all singing "A Whole New World", most VAs taking requests for other character voices, and Squigly's formal debut and the VAs' reactions to it.
The videos for the stream can be found here, with most of the events happening in this one.
Filia and Squigly meet in the latter's story mode. Filia tells Squigly & Leviathan that she's lost her memory and Samson is helping her find the Skull Heart so she can use it to get better. Leviathan accuses Samson of taking advantage of Filia's amnesia, manipulating her into going after the Skull Heart because he wants her to be turned into a Skullgirl. Samson responds by being a jerkass, insulting Squigly's appearance and then telling Filia that she can't trust this undead chick, who has to be working for the Skullgirl. At this point both Squigly and Leviathanwant to beat the crap out of Samson.