Terrence of KateModern is an unusual case. When he reinvents himself as bespectacled, cardigan-sporting religious nerd Terry, he is recognised almost immediately by the other characters. However, it somehow doesn't occur to them that quiet, harmless Terry might be the murderer. He later plays this trope straight as the straight-jacketed Patient # 12.
In Englishman, Englishman's Battle Butler (known only as Butler) also moonlights as a supervillain named The Butler. A deconstructed example in that it appears to play this trope straight (E'man never connects the two) but Butler never actually bothers with a disguise, rendering it PoMo.
One CollegeHumor video that is a Take That! to British Petroleum's response to the 2010 Gulf oil spill. Tony Hayward and other executives in BP's London offices announce to us after showing a video from an actual focus group that cleaning up the spill is difficult, so they're changing their name to Baby Otter Smiles & Co. Then Hayward and another employee Sally Harris (both of whom are wearing false mustaches) go to the house of Sarah Schneider (one of the focus group members). Sarah sees through their cover, says "You guys are monsters," and shuts the door on them.
In Dr. Horrible's Sing-Along Blog, the titular character is not recognized by Penny, even though neither his "costume" or his "normal" clothes covers his face (he wears goggles, but only on his forehead until the last scene).
Possibly justified in that Penny may or may not have heard of Dr. Horrible: she never mentions him; he's not a big time supervillain (yet), and the first time she sees him, she is under a lot of stress, but still ends up recognizing him.
Most people miss Billy in the back of the soup kitchen as he glowers at Captain Hammer and pours the soup back into the pot instead of into the bowl. The irony of it is his idea of disguise is wearing a fake mustache and an apron and not wearing his hoodie.
Paper-Thin Disguise is also subverted, as when Captain Hammer meets Billy, he instantly recognises him as Dr Horrible.
Linkara: Oh wait! I know this one! You're That Chick With The Goggles! Dr. Insano: But- but- I'm not freaking black! Linkara: But you are a woman, eh?
Subverted in Kickassia, when several people recognize Spoony as "that doctor guy", despite his frantic efforts to pretend he's not Insano.
Another Kickassia example. During the invasion the President of Molossia Kevin Baugh disguises himself as Baron Fritz von Baugh, Minister of Making Things Orderly. Although it is obviously him in disguise The Nostalgia Critic decides to go along with it and lets him stay. As it turned out doing this was a huge mistake on the Critic's part as it allowed Baugh the chance to plant a Apple of Discord within his already grumbling troops further sabotaging the future of Kickassia.
In The Time... Guys episode with Timmy's Spanish teacher, Señor Tigre instantly realises that Dr. Chronos is not Timmy's father when Doc takes his multiple neckties off.
This is used for fun when Lindsey Stirlingdresses up as her own #1 fan Phelba — the only disguise is that Phelba has thick black glasses. A few people realize that Phelba and Lindsey look rather too similar, but since not enough people know them it generally works.
On certain "Sparks Nevada, Marshal on Mars" segments we encounter Jib Janeen, an alien shapeshifter. Although Jib Janeen is always voiced by Paul F. Tompkins and speaks in the same goofy, high pitched tone regardless of who he's impersonating, the other characters are always completely fooled by the impersonation.
"Desdemona Hughes, Diva Detective" features former actress Desdemona Hughes adopting various disguises to solve murders. The disguises are apparently legitimately good and, unlike Jib Janeen, Desdemona alters her voice to suit her impersonations. She also makes a very conspicuous show of leaving right before assuming a new disguise and the disguise is often of someone or something completely random and unrelated to the current situation. Nobody is ever suspicious for long and are always surprised at the actual reveal.
In We Are All Pokémon Trainers, Lenore (Fool's Hydreigon) and Ammy (Umbra's Volcarona) use these to get their own Trainer's Licenses - they wore fake moustaches and trenchcoats. Lenore's case is more notable, because while Ammy was wearing a Pokémon-to-Human armband, Lenore wore the disguise as a Hydreigon.
John: I must say, you rather remind me of someone. Derek: Well, I am well known locally. John: Your telescopic manipulator arm in particular looks familiar. Derek: What? This little thing? (boing) John: Yes. Derek: It's just there for show, John! JUST! THERE!! FOR!! SHOW!!! [exterminates a tree]
Scary News out of Tokyo-3: Anyone with any knowledge of Neon Genesis Evangelion should be able to guess immediately who's hiding behind the transparent handle of "ZeroGirl00" — she's one of several characters with inside knowledge of NERV, and she has a tendency to talk about the Pilots in I Have This Friend... fashion. Unfortunately, none of the other characters are in a position to figure out the truth until she finally comes out and admits it.