- For a brief second in the Columbia Pictures logo, Torch Lady turns into Jane Fonda as Cat Ballou. This is taken directly from that film's Logo Joke. And for an even briefer second, the logo shifts to the Logo Joke from Cloudy with a Chance of Meatballs, with the Torch Lady getting flattened by a giant banana.
- The logo and title font changes much like the title sequence in the movie Enter the Void, and were explicitly inspired by it.
- Miles's room includes a poster of the album cover from Chance the Rapper's Coloring Book, except Chance's hat now says "4" instead of "3" on it.
- The license plate number on Jefferson's police car is "RFD 690", which is a common New York prop plate number appearing in various media, the most notable example being the lead detectives' car in Law & Order.
- One of the mooksnote fighting Peter B. Parker in his flashback montage looks suspiciously like Steven Seagal, complete with ponytail.
- Peni Parker's SP//dr robot is a cutesy, curved animal-themed mech, just like D.Va's own mech, from Overwatch. She's a Gadgeteer Genius who likes popping snacks inside her mech, to boot.
- Peter Porker's animation and design looks reminiscent of the Looney Tunes. He also looks similar to Porky Pig. Even lampshaded when the Spiders return to their home dimensions. Porker's parting words are "That's all, folks!", prompting an immediate reply from Peter: "Can he say that? You know, legally?"
- In the background of Gwen's reality in her focus trailer is a poster advertising Clone College, with Abe and JFK in similar positions to the poster for 22 Jump Street.
- Some billboards seen in Times Square include a Shaun of the Dead sequel called "From Dusk Till Shaun", which was what the creators of the original film said a potential sequel would be called.
- Another billboard is advertising Hi, Hello, a riff on the actual ''Oh, Hello" show starring Nick Kroll and John Mulaney.
- Yet another billboard advertises a film called "Baby Shower" which seems to be that universe's Bridesmaids.
- Aaron has Community on his TV, specifically a scene from season 2 where Troy gets out of bed in a Spider-man shirt. Donald Glover who plays Troy was partly the inspiration for Miles Morales, voiced him in past adaptations, and played Aaron in Spider-Man: Homecoming.
- The Spider-Lair is obviously modeled on the Batcave from the DC Animated Universe down to the display array of multiple Spider-Man costumes with blue lighting illuminating them.
- The scene of Miles in free fall against the neon cityscape is very reminiscent of the iconic free-fall scene from Ghost in the Shell.
- During the ending, Miles zips past a store called "Perry Joe", a reference to Joe Perry, lead guitarist of Aerosmith and noted Spider-Man fan who performed the theme song for Spider-Man: The Animated Series.
- The Prowler is clearly channeling the Predator. His main role in the story is a relentless hunter chasing after Miles, and he has the ability to see in multiple light spectrums. There's even several scenes where the audience sees through his eyes, and it's much the same as how the Predator sees through his mask.
- Some moments with Noir, especially when it's in his universe, stylistically take very much after Sin City.
- The visuals of the city flying apart in the Super-Collider draw from AKIRA.
Shout Out / Spider-Man: Into the Spider-Verse