Some of these works have characters coming out or being shown to be LGBTQIA+ as reveals, beware of spoilers.
See LGBT Representation in Media for a list of works in other mediums with prominent LGBTQIA+ representation.
See also Queer Media for works with a primary focus on LGBT people, queer subjects and themes and Queer Romance for works that focus on romantic relationships between queer characters as the main plot. See also LGBT+ Creators for a list of artists/media creators who are LGBTQIA+.
- Ace in Space: The main protagonist is a nonbinary panromantic asexual, whose love interests are primarily sentient robots that lack gender identities; the exception is a human male from their home planet.
- Adastra (2018): With the exception of Cato, every major male character in the story is gay.
- Ascension (2021): Escort is nonbinary, and the other two main characters are heavily implied to be transgender.
- BAD END THEATER: The Maiden and the Overlord, who are both women, have clear feelings for each other if allowed to interact, and the Omega Ending reveals that they're based on TRAGEDY and the player, respectively, two separated sapphics. The Underling and the other minor demons are nonbinary.
- Being A ΔΙΚ: MFF Three-Way Sex with several girls aside, Maya and Josy's (both bisexual) relationship is central to the plot regardless of player choice, and Tremolo may enter a polyamorous relationship with them depending on choices made.
- Blooming Panic: Besides all the romance options being open regardless of the protagonist's gender, NakedToaster is nonbinary and uses he/they pronouns. Among the supporting characters, two2 also uses he/they pronouns while BIGLADY is a lesbian.
- C14 Dating:
- Existence of a Gay Option aside, two of Melissa's potential male love interests act Ambiguously Bi towards each other. Yet another of the men turns out to be a heteroromantic asexual.
- There is a lesbian in the supporting cast and another character mentions going to visit a male friend "and his husband".
- Choices: Stories You Play: A collection of various stories that you might have a relationship with a love interest of the same gender. More specifically:
- The Freshman: Kaitlyn develops a crush on the main character and comes out to her parents as a lesbian in Book 2. The main character's roommate is gay, and part of Book 4 involves helping him move on after he breaks up with his boyfriend.
- High School Story features the first nonbinary character in the Choices franchise, Cameron Levy.
- It Lives:
- One of the main characters in the first book, Lily, is a lesbian, having a crush on her bully. She can forgive her bully in the end or realise that she's not worth it and find a girl who genuinely likes her for her. Andy is transgender (the first ever trans character in Choices, in fact), and is bullied by most of the basketball team for who he is (to the point where they only recruited him because it would get them good PR). If he survives the events of the story, the basketball team begins to treat him with respect.
- In the second book, the main character's brother is gay, along with his boyfriend (who's also the son of an antagonist who killed their parents, kicking off the events of the story).
- It Lives Within (the Fan Sequel) has Luis (an openly bi/pan witch who not only flirts with the main character regardless of their gender, but also compliments their love interests' beauty), Sunny (a non-binary witch from the same coven as Luis, who is also mentioned to be aro-ace in Chapter 19), and Amalia (one of the main character's love interests, who is canonically demisexual, mentioned in Chapter 14).
- The Elementalists: Zeph is the first canonically asexual/aromatic character in the Choices franchise. As he puts it, he's not into people romantically or sexually, just "friendically". Also, this is the first Choices book where the main character's sexuality can be explicitly customized. If the main character is also aro-ace, they are given an option to mention it to Zeph when his sexuality is brought up.
- Bachelorette Party: One of the main character's close friends is a trans woman who came out in college.
- Blades of Light & Shadow: One of the main character's love interests, Mal, has a sister with a wife. Two of the female Old Gods were said to be deeply in love with each other (until one banished the other for messing with her plans), while two members of the Shadow Court (Lady Nyra and Baroness Isador) are also a lesbian couple. All goblins are stated to be non-binary by Word of God, and one of the Ashen children also uses they/them pronouns. The main character is (or can be potentially played as) omnisexual.
- My Two First Loves: One of the main character's love interests is her lesbian best friend, and it took 38 chapters for the latter to come out.
- Murder at Homecoming: This book deals with LGBTQIA+ issues quite extensively, including the trials and tribulations faced by the LGBTQIA+ community. The murder victim was in a relationship with a girl who couldn't make their relationship known because of the girl's extremely homophobic parents (in fact, said girl was sent to conversion therapy when she was 12). Also, two of the main character's love interests are canonically bisexual, while the third comes off as straight, then he discovers he isn't if a male or nonbinary main character romances him.
- Getaway Girls: This book also tries to fully represent the LGBTQIA+ community. The 4 playable characters come in various sexualities: one straight, one lesbian, a main character who can also be a lesbian or bisexual (if the player so chooses) and the second canonically asexual/aromatic character in the Choices franchise (who also happens to be the main character's sister).
- Kindred: The main character's sister is transgender and sapphic, and part of the story revolves around her complicated feelings for her dead mother, believing that she didn't love her for who she was (it turns out that her mother thought that having three daughters was a bad omen).
- Alpha: Two of the main characters' fraternity buddies, Asher and Lupe, are gay (with the former having a boyfriend named Cal, and the latter having a crush on a sorority sister named Emmy).
- The Deadliest Game: Two of the love interests (Farah and Dante) are canonically bisexual, the latter talks about how the queer representation in his upcoming movie role is important to him.
- Class of '09: Nicole, the protagonist, is bisexual, and there are other lesbian and bisexual characters.
- Crown Delights Deli: Two of your regular customers, Maya and Piper, are an LGBT+ couple who first visit you on Pride Day. Maya wears the Progress Pride flag on her tank top while Piper wears a jacket with pansexual colors and a genderfluid flag pin.
- Cryptid Crush: The protagonist is nonbinary and you can chose their pronouns, and there is another nonbinary main party member, Jamie.
- Dream Daddy: Your player character is a single father who finds himself in a new neighborhood full of other single dads (plus one in an unhappy marriage) who - you guessed it - you can have him date. While no character's orientation is specifically labeled, beyond the options all of course being MLM, Hugo mentions an ex-husband, while Joseph has a wife, Craig has an ex-wife, and both Mat and Robert are widowers who were previously married to women; plus Robert's daughter Valerie mentions having a girlfriend. Another option, Damien, is transgender. All of the previous can apply to your character, too, as you can code him as trans by dressing him in a binder while customizing his sprite and an early dialogue choice prompts you to choose whether his own deceased former spouse was a woman or a man.
- Echo: Almost all of the main characters are men attracted to other men, exclusively or not, with the story taking place in a small, homophobic town in the American southwest.
- The Smoke Room (prequel to Echo)
- Arches, the sequel, notably has a gay couple as the dual protagonists.
- Far Beyond the World: Part of the story is the growing romance between Ranok and Caelan that the two need to keep secret, as LGBTQIA+ people are seen as "defective" in the wolves' society.
- Fate/stay night: In the uncensored version, tritagonist Rin Tohsaka is bisexual and discovers this during a sex scene with deuteragonist Saber (a girl) and protagonist Shirou (a guy); the rest of the story has her continue expressing affection towards Saber while also being able to have sex with Shirou. The censored version and later adaptations, however, downplay references to her sexuality, rendering her Ambiguously Bi there.
- First Kiss at a Spooky Soiree: The protagonist Marzipan is panromantic, and there are four girls and four boys she can kiss in the game's Multiple Endings.
- Heaven Will Be Mine: Most of the cast is some variety of LGBTQIA+, with most being lesbians, though Mercury and Ganymede are two men married to each other.
- Katawa Shoujo: Misha, the only female major character Hisao cannot date, is in love with Shizune, the deaf-mute woman she interprets for.
- Lands of Fire: The main character is gay and has multiple love interests. Uniquely it takes place in pre-colonial Australia, so it explores the sexual norms of Aboriginal cultures.
- Ladykiller in a Bind: The main character is a butch lesbian biker, and you have the option to romance several of the female characters (and a few male ones, though she's only doing so in that case because she's disguised as her brother and she needs to keep up the charade).
- Life After Magic: Player character Akiko is m-spec with a preference for women; she occasionally muses about a past crush on her male acquaintance, Solaire, but the romanceable characters in the game all include sapphic women ARA, Jackie, Miranda and Leez, and nonbinary KJ. In a side-story, Akiko can also ask out Anna Lee - who is implied to have been in love with her fellow magical girl Saori - but she will turn down Akiko due to the age gap.
- Long Live the Queen: Four of the female characters turn out to be lesbian if the right gameplay choices are made. Elodie can make one of them her lifelong companion, either unofficially or officially. Another one can only be made an unofficial one. One snippet of the country's history that can be learned involves a nobleman who gave up on marriage to pledge himself as the lifemate of the man he was in love with.
- Lovelink: Some of the possible matches are non-binary.
- Magical Warrior Diamond Heart: Pansexual female main character, multiple bi and lesbian supporting characters/love interests, non-binary supporting character/love interest, trans male supporting character/love interest and aromantic/asexual supporting character.
- Melody: Sophia and Xianne end up as a couple, but not much attention is paid to their relationship.
- Mice Tea: The main protagonist is bisexual, with male and female romance options. Two of her love interests are trans women, with one having transitioned prior to the story's events, and the other initially identifying as male but whose route centers on her transition; during the latter's route, the male love interest uses magic to experiment with gender and sexual identity, but ultimately identifies as cisgender and heterosexual.
- Missed Messages: The player character is a lesbian trans woman, with the option to date one of the female characters depending on your choices.
- Monster Prom series: Player characters can identify as male, female, or nonbinary regardless of their appearance, and this choice does not block access to any of the love interests. Main romance options and multiple side characters are LGBTQIA+: a male demon has two dads; a female witch has male, female, and nonbinary ex-lovers; one monster is an asexual aromantic that players befriend instead of date; there's a nonbinary soul reaper; and so on.
- Murder By Numbers: One of the major characters is a gay man, and one of the murders you investigate takes place at a gay bar.
- one night, hot springs: Haru is a transgender girl with a crush on Manami, Erika is bisexual, and Manami eventually discovers that she is aro-ace.
- Red Lilies: Irene is a sapphic girl attending an all-girls school in the Victorian era, all of her love interests are girls.
- Repurpose: The main protagonist can be cis male, cis female, trans male, or trans female. Most love interests are either bi or pansexual; the exceptions are a lesbian woman, an asexual angel, and a demon woman whose heterosexuality explicitly does not exclude trans men. One character, an omnisexual angel, has no set gender identity and can change their form to appear male, female, or nonbinary depending on the player's preference.
- Romance Detective: Almost everyone in Lovebloom City is gay, and the plot revolves around the player and her partner/girlfriend solving love-related mysteries and crimes.
- Sex Advice Succubus: The main character's clients include a ghost girl asking for advice on being intimate with her girlfriend, and a male incubus uncomfortable with sex who can be advised to research asexuality.
- Stillwater: The VN follows the story of a detective investigating strange occurrences in the mansion of an elderly man. Said elderly man is later revealed to be haunted by the ghost of an old male lover he lost in his youth.
- Syrup and the Ultimate Sweet: Toffee, one of the main characters, is nonbinary. The protagonist Syrup is a girl who likes girls, which is only alluded to in the game itself, but confirmed in NomnomNami's webcomic.
- Va 11 Hall A: Protagonist Jill is bisexual and a large part of her plot revolves around grieving her deceased ex-girlfriend and getting together with her boss, Dana. Other queer characters include Mario, and, possibly, Sei and Stella.
- Wicked Willow: The main protagonist is a lesbian living in the '90s, an era when the community was still looked down upon. Some routes discuss the consequences from being open about her sexuality as she grows attracted to the women in her life.
