Spiders and Magic: Rise of Spider-Mane (also read here) is a Spider-Man/My Little Pony: Friendship Is Magic crossover fanfic by Maximus_Reborn. Trouble is brewing, and Spider-Man is summoned to save the day! However, he takes the form of a pony upon his arrival and slowly starts to lose his powers. Now as Peter Pony Parker, he must become Equestria's new hero as the Amazing Spider-Mane, but with past failures haunting his mind, will somepony be able to give him the happiness he deserves and save him from a prophecy that ends with his death?Across Equestria and through Earth, two hearts will join together, but one may die to ensure the other's survival...Still ongoing, as of 2013.
Ascended Fangirl: Twilight Velvet is a big fan of Spider-Mane. And now her daughter is his marefriend. Oh my.
Action Girl: Twilight shows more traits of this in the Marvel Universe.
The Mane Six in general.
Princess Luna also gets a chance to show her power.
Alternate Continuity: The Spider-Man of this fanfic seems to have elements from both, the mainstream Marvel universe, and the Ultimate universe, as well as some elements from the films. (The final consensus seems to be that he's the "Amazing" Spider-Man from Shattered Dimensions, though Edge of Time doesn't seem to have happened yet.) It is also an alternate continuation of MLP Season 3, since it takes place after Sombra's first defeat in the Crystal Empire.
Darker and Edgier: Obviously, when compared with MLP:FIM, especially since it incorporates some elements from the Ultimate Marvel universe (which loves this trope). For the most part, however, it's a pretty light story.
Defrosting Ice Queen: There is a non-romantic example, with Peter trying to win Princess Luna's trust, and even teaching her to loosen up a little in the process. The non-romantic part starts to get a little iffy after he succeeds, especially in chapter 35.
Death Glare: Blueblood is given one by most of the Mane Six in Chapter 40, after he insults Peter.
The Determinator: Spider-Man, obviously. When the ponies start to learn more about his past and his reputation back in New York, they can't help but admire his willingness to keep fighting for people that distrust him or outright hate him.
Also, Rainbow Dash, when trying to prove that she's a better hero than Pete, and going as far as to enter into a dragon's lair as a "Test of Bravery".
And Lyra, and her obsession with proving that Spider-Mane is a human.
Leotard of Power: Part of Twilight's costume after her trip to Earth with Peter and Spike.
Le Parkour: Since the buildings in Ponyville tend to be only two stories high, this is Spider-Mane's main form to move around.
Let's Get Dangerous: While Equestria citizens admire Spider-Mane, they won't become aware of his full power until his seemingly hopeless duel with the Princesses of the moon and the sun. Hopeless, indeed.
Second Love: Twilight, especially since Word of God states that she serves as the Mary-Jane of this story (except that Twilight is more capable of defending herself). Funnily enough, Applejack, who has had the least Ship Tease with Spidey (aside from Pinkie Pie), reminds him of Gwen.
Shout-Out: Peter does this quite a few times, much to the confusion (and in Twilight's case, annoyance) of Equestria's denizens, since they can't get any of the references. True to his usual style, he continues LONG after Twilight voices her displeasure... or because of it.
Chapter 43 ends with the intro Marvel Ultimate Alliance without Spider-Man.
Also, Princess Luna and Rainbow Dash to a lesser extent.
Weak, but Skilled: The cast's assessment of Spidey when he's going against Spitfire. Spitfire is naturally faster, but Peter has his Spider Sense and the acrobatic agility to go full speed at all times while she has to slow down. At all OTHER times though, this does not take.