Anime & Manga
- The 100 Girlfriends Who Really, Really, Really, Really, Really Love You: In Chapter 47, Hahari compares the chastity belt Rentarou is wearing to Astro Boy's briefs.
- Black Jack: In Kimba, Astro Boy cameos twice. Once he's in a world's fair as a model robot, the other time one of the villains is actually WATCHING Astro Boy beat up one of his enemies!
- High School Of The Dead: In the manga: when Saya explains what the side effect of nuclear explosions is, electronic appliances no longer working, there are pictures of said appliances. Among them there is a robot that looks similar to Astro Boy.
- Nichijou: The Professor seems to be a fan of Astro Boy if Nano's adventures in Chapter 178 are anything to go by.
- Scott Pilgrim vs. The World: In the scene where Stephen Stills mentions "distressing news", Scott wears a Tetsuwan Atom era Astro Boy T-shirt.
- Calvin and Hobbes: Hobbes once does Calvin's hair for school picture day. Calvin keeps asking him how it looks and Hobbes finally tells him that he looks like Astro Boy. Upon hearing this Calvin exclaims that he can't wait to get his picture taken.
- Disgaea: Many of the random names in Disgaea 4: A Promise Unforgotten/Revisited are references to other media. Kanaya West, Niptoonia, Nicolas Gage, Astrogirl, Tigerblood, Churrozard... and that's not even counting names that might be considered common enough to not be direct references, like Snake, Tifa and Epona.
- Phoenix Wright: Ace Attorney -- Justice For All: Phoenix and Pearl find a toy robot bear in 2-4, and Pearl asks how much horsepower it has.
- Tatsunoko vs. Capcom: ** Roll also invites Casshan and Friender to come visit Mega Man and Rush (Mega Man and Rush were directly inspired by Casshern, Friender and Astro Boy).
- Neptunia Victory: One of Izawa's trailers show off a lot of works from Osamu Tezuka, such as Black Jack and Astro Boy.
- Persona 3: Nata Taishi's design is clearly based on Astro Boy.
- Persona 4: The Persona Nata Taishi is clearly patterned after Astro Boy.
- Pokémon: Mewtwo's backstory in Pokémon: The First Movie is one big shout out to Astro Boy. Both involve similar-looking scientists whose children died in car accidents (Amber's only being depicted in the original Radio Drama). The scientists react by creating a Replacement Goldfish, but end up failing (AmberTwo died, while Astro was not a perfect replica of Tobio).
- Tekken: Alisa has traits matching Astro Boy.
- DuckTales (2017): The episode titled "Astro B.O.Y.D.!" is unabashed of it's inspiration.
- B.O.Y.D.'s original name, 2BO, is a reference to Astro's real name Tobio.
- Gyro clashes with an Inspector Tezuka, named for the legendary manga artist Osamu Tezuka who created Astro Boy.
- OK K.O.! Let's Be Heroes, "Sidekick Scouts": Combo Breaker is an obvious Astro Boy parody, sharing the same haircut and attire and being a cyborg with a wide array of gadgets. When he draws what his "dad" looks like, it's clearly meant to be Professor Ochanomizu/Elefun.
- The Powerpuff Girls (2016): Professor Newtonium's "perfect little boy" is an expy of Astro from Astro Boy.
- Steven Universe, "Chille Tid": In Steven's second dream, he flies with a propeller attached to his head. After a power boost from Pearl's giant floating head, he soars like Astro Boy.
- The Venture Bros.: The members of Doc's therapy group are made up of other boy adventurers. Such as "Action Johnny" (Jonny Quest), Lance and Dale Hale (The Hardy Boys), Roboy (Astro Boy), and Wonder Boy (Robin, the Boy Wonder).
- Teenage Mutant Ninja Turtles (2012): Previews for season 2 show a Kraang with "butt cannons".