In 2001, Devil's Due Publishing (a spinoff of Image Comics) acquired the comic rights to G.I. Joe and launched a new series, set in the same continuity as the Marvel Comic. They published an ongoing title - once again called G.I. Joe: A Real American Hero, but later renamed G.I. Joe: America's Elite - and several miniseries exploring the backstories of the characters. Devil's Due lost the comic rights to IDW Publishing in 2008, and their final issue was printed that July. The Devil's Due run has since been republished by IDW under the Disavowed banner, a reference to its non-canonical status following their decision to make a separate continuation of the original Marvel series.
- Adult Fear: The issue about Hannibal and the other young Serpentor clones. The reactions of the clones' adoptive parents are really understandable as their children are lost.
- Ascended Extra: Dr Knox was just an one-shot Cobra scientist from the tail end of the original Marvel run. After Dr Mindbender is Killed Off for Real, she becomes Cobra's main scientist.
- Canon Discontinuity: Originally the Devil's Due's run of G.I. Joe was promoted as a legitimate continuation of the original Marvel series, but when Devil's Due was set to lose the rights to the IP to IDW in 2008, they concluded their run with the World War III story arc, which left little room for any follow-up, forcing IDW to reboot the property. IDW later brought in Larry Hama to write his own sequel to the Marvel series, which ignored the Devil's Due run.
- Comic-Book Time: Averted at first, as the first Devil's Due volume stated that the Joes fought in the 80s and their age showed from time to time. Later it was played straight and the Joes were portrayed younger again.
- Dying Moment of Awesome: Flash and Mainframe blow themselves up, along with a number of Coil agents.Flash: Mission Accomplished.
- Enemy Civil War: Cobra vs the Coil. While the Joes also face the Coil, they refuse Destro's offer for an alliance against the Coil.
- Evil Chancellor: White House Chief of Staff Garrett Freedlowe - actually Cobra Commander in disguise - tries to have the President replace G.I. Joe with the Phoenix Guard, a unit secretly made up of Cobra agents.
- Formerly Fit: Bazooka is portrayed as this when he volunteers to return to active duty. Having spent the seven years since the Joes disbanded, he is overweight and out of shape (and balding). He later hits the gym and gets back into shape.
- Legacy Character: Both Croc Master and Sneak Peek get successors. They share the fate of their predecessors, and Croc Master II is even killed in his debut issue.
- Nanomachines: The first Devil's Due saga had Destro or rather his son Alexander using Nanomites to take control of Cobra.
- No-Dialogue Episode: The Devil's Due series also had one, as a homage to the Marvel series. Just like the Marvel issue, it was issue #21.
- Reed Richards Is Useless: The very first storyline in the series centers around a nanomachine attack. It infects people and begins to slowly destroy their bodies from the inside, using them as raw materials for building more nanomachines. The "cure" developed for this is to reprogram the nanomachines to disassemble each other instead, and build back up the organic matter of the patients molecule by molecule, up to and including complete, fully functional cells. This should logically have tremendous medical applications, which is never brought up.
- Remember the New Guy?: Big Boa and Copperhead appear as veteran Cobra members, even though they didn't appear before in the American comic book. If that isn't this tropes, then it's Broad Strokes with the Marvel UK comics where they actually appeared.
- Sequel Series: The series was originally an official continuation of the Marvel Comics continuity, but, as stated above, has been rendered non-canon by IDW doing their own continuation.
- Supreme Chef: Roadblock is still this, and he even develops the "Marvin Hinton Grill" and when overeager Dreadnoks try to destroy the studio, Roadblock fights them on stage, which increases his popularity (and grill sales).
- Thicker Than Water: When Zartan finds that the Coil agent he just hurt is his brother Zandar, he takes him and withdraws all Dreadnoks from the battlefield.
- Toothbrush Floor Scrubbing: Drill Sergeant Nasty Beachhead tells a recruit that a cruel man would make him clean the barracks with a toothbrush, before making him clean the barracks with a POTATO.
- Weather-Control Machine: The Tempest is a more subdued variant of this trope which can start rainstorms anymore, but not cause natural disasters like the cartoons Weather Dominator could. Thats fine for Cobra though, who use it to create deadly acid rainstorms.