My Little Pony Equestria Girls is a Generation 4 movie made by the same studios as Friendship Is Magic, set between that show's third and fourth seasons.The concept is simple enough: take the characters of the hit TV show My Little Pony: Friendship Is Magic and put them in high school. Now the Mane Six must deal with things such as peer pressure, planning for school dances, popular girls with attitude problems, and yes... even boys.Sounds simple, right? Did we mention the pastel-colored ponies are now pastel-coloredhumans?This spin-off franchise is something of a grand experiment for Hasbro and their attempt to break into the growing market of alternative dolls. Can you have My Little Pony without the Pony? Is Friendship still Magic? Right now, those questions are a matter of debate, but Hasbro certainly hopes so.The movie centers around Twilight Sparkle and Spike going into an Alternate Universe when the Element of Magic is stolen by Princess Celestia's former apprentice Sunset Shimmer, turning into a teenage human girl and a talking dog respectively in the process. Along the way Twilight meets human versions of her friends from Ponyville, all of whom turn out to despise each other, and a blue-haired fellow who seems like a nice enough guy. With the help of her new friends in this strange new world they must find the crown and change the destiny of the two parallel worlds.See the firsttwo trailers.The movie was written by Meghan McCarthy, premiered at the Los Angeles Film Festival on June 16, 2013, and had a limited theatrical run at over 200 US and Canadian theaters in June and July of the same year. It was released on DVD and Blu-ray in the US by Shout! Factory on August 6, 2013 and will be released on DVD in other markets worldwide, and aired later still on The Hub on September 1st, 2013.IDW put out a prequel comic as the first issue of their My Little Pony Annual series, with the 8 page short about Sunset Shimmer's origins printed early in IDW's MLPFIM #9 variant at 2013 SDCC.A sequel, My Little Pony Equestria Girls: Rainbow Rocks, was announced in February 2014 with a planned release in Fall 2014.A new French-made series, based off the Equestria girls line, Equestria academy, is rumored to be in the works and released somewhere around early 2015.Not to be confused with the commercial for Friendship Is Magic or the parody YouTube videos of the same name.
This animated film provides examples of:
Absentee Actor: Despite being initially set in the Crystal Empire, and with Princess Cadance present, Shining Armor is nowhere to be found, either in the Crystal Empire or in human form. Princess Cadance is also the only main character present in the film without a human equivalent.
Accidental Pun: Snips and Snails complete their "What Are Little Boys Made Of?" trio (snips, snails and puppy dog tails) when they kidnap the puppy version of Spike.
Age Lift: Several characters from Friendship Is Magic, including the Cutie Mark Crusaders, Snips and Snails, and a few other younger cast members from are aged up to at least resemble high-school freshmen. Conversely, other adult characters who live on their own, and in some cases even run their own businesses, are aged down to meet this criteria.
All There in the Script: During Twilight and Fluttershy's discussion of the Fall Formal Princess, Fluttershy says that the previous girl to go up against Sunset was utterly humiliated, but she never says who it was. According toWord of Saint Paul, it was Rarity, though a few lines imply that it was her.
Alpha Bitch: Sunset Shimmer. Humiliates anyone who stands in her way to become Fall Formal Princess? Check. Segregates the entire school into smaller social groups to ensure they don't band against her? Check. Destroys long-lasting friendships just for kicks? Check. Good thing she pulls a Heel-Face Turn at the end.
Alternate Self: Twilight Sparkle meets AU counterparts of her friends who are attending high school together. AU Pinkie asks Twilight if she had a twin since she vaguely recalls having seen someone similar with a purple and green dog named Spike in the city, confirming that an AU counterpart of Twilight does exist.
Amazing Technicolor Population: The vast majority of the human characters still use the ponies' wide array of color schemes, though some still fall into normal human skintone ranges by coincidence, most notably Applejack. Big Macintosh and Celestia are standout exceptions, having Caucasian skintones instead of their normal apple red and pure white, respectively. Similarly, Cheerilee and Luna have their coat/hair color schemes reversed because their coats would still be overly dark on screen even if lightened a bit like the other characters.
Ambition Is Evil: According to the tie-in comic, Sunset Shimmer's fall from grace began when lured with the power that comes with becoming an alicorn and ruling Equestria, blinding her into believing she can do no wrong.
Amulet of Concentrated Awesome: Twilight's Element of Magic serves this function in the human world. While unicorn magic is nullified going through the portal, the Element retains its power through the transition.
Animal-Eared Headband: Worn by the human Applejack, Fluttershy, Pinkie Pie, Rarity, and Rainbow Dash as part of the school's cheerleader/mascot outfits, along with makeshift pony-like tails worn around their waists.
Apple of Discord: Long before Twilight arrived, Sunset Shimmer broke the friendship of the human versions of Applejack, Fluttershy, Pinkie Pie, Rarity, and Rainbow Dash through misinformation: Rainbow Dash was given the wrong date for Applejack's bakesale; Pinkie Pie was told via text to throw a huge party that would scare Fluttershy's animals; and Rarity was told via emails that Pinkie Pie didn't need help setting up decorations for various school functions due to having enough volunteers only to learn later Pinkie set the decorations all up by herself.
Attack of the Political Ad: Sunset's video of Twilight, right down to using color-negative shots at the goofiest moments with heavy musical beats.
Audience Shift: Compared to its parent series, this iteration is geared towards older girls, mostly between the ages of 8 to 12.
Big "Shut Up!": Twilight shouts "STOP!" when her friends' arguing becomes too much for her to put up with.
Big "WHAT?!": Human!Pinkie delivers two in a row when Twilight tells her friends that the Fall Formal must be moved to tomorrow night because Sunset Shimmer ruined Pinkie's decorations for the formal. She tries to do it a third time when Twilight insists the formal must go on tonight, but Applejack slaps her hand across Pinkie's mouth.
Bitch in Sheep's Clothing: Sunset Shimmer's mode of operation, at least when she is dealing with someone she can't intimidate into submissionnote She can pretty much boss any of the students around, and since the faculty isn't featured much in this movie. You see it once or twice, and it's pointed out by Applejack.
When human Fluttershy first meets Spike, she remarks that she would love to know what he or other animals were thinking. No points for guessing what she asks immediately upon finding out Spike can talk.
A double whammy: Sunset Shimmer tells Snips and Snails to get dirt on Twilight like they did for "the last girl who thought she could challenge me". Later, Fluttershy remarks that Shimmer did the same thing to someone who ran against her at the "Spring Fling" dance. Turns out it was Rarity, who at the end of the movie, remarks that she expects an apology for "last spring's debacle".
Broad Strokes: The movie's canon status regarding the series is a bit vague, especially given the lack of pony Flash Sentry. He only has two appearances in season 4, and even then the first one was just thrown in by an animator with no input from anyone else on the staff.
Care Bear Stare: In the finale, the girls hit Sunset with a giant Rainbow blast which transforms her back to normal, bawling and apologizing for what she did. Very much like the rainbow-de-brainwash-beam from the Trope Namer franchise.
Cat Smile: In a blink-and-you'll-miss-it moment during Pinkie's solo in the "Helping Twilight Win the Crown" musical, Pinkie manages to give a faint one while moving towards the camera.
Celestial Deadline: The portal only remains open for three days, and then closes for thirty moons when the moon reaches its peak on the third day.
Chaos Architecture: Canterlot High for a brief moment — Twilight sees roughly five windows on one wall, yet when she runs towards the school, runs past a lot more than five.
Co-Dragons: The human counterparts of Snips and Snails function as Co-Dragons for Sunset Shimmer.
Composite Character: Sunset Shimmer has similarities with several antagonists from the original show. She was once close to Celestia and is beaten by the Elements of Harmony like Nightmare Moon, is a smug Evil Counterpart to Twilight Sparkle like Trixie, drives the Mane Six apart like Discord, attempts to discredit Twilight like Queen Chrysalis, and using magic to enslave the school in a manner similar to how King Sombra took over the Crystal Empire.
The CMC upload a video of them singing the same song from "The Show Stoppers" onto an Expy of YouTube. Cheerilee moves to cut the volume right before the last word of the lyric "When you're a younger pony..."
When the girls rally together to fix the messed-up gym, Fluttershy lets out a faint "Yay!"
Diamond Tiara and Silver Spoon do their signature "Bump bump sugar lump rump" dance at the Fall Formal.
Principal Celestia says "Happy Thursday" in her morning announcement the same way Princess Celestia said "Happy Tuesday" in "It's About Time".
Contrived Coincidence: Events on both sides of the mirror require an unbelievable amount of good timing, none of which can be said to be planned by any one party. The Fall Formal just happens to lie within the active period of the mirror, the mirror happens to be in the Crystal Empire, Twilight happens to have been summoned there for an all-princess summit during the same time, and Sunset Shimmer somehow knows all of this and is able to track down Twilight within the castle despite living in a world which (as far as is made clear) has absolutely no magic except the portal which is rarely active.
Cool Car: Flash Sentry shows up to the dance in a black muscle car, with lightning bolt decals and his T-shirt emblem/cutie mark emblazoned on the hood. And just for kicks, it's similar in design to a modern Ford Mustang.
Cool Crown: Twilight's crown, which turns out to be a redesigned Element of Magic.
Twilight Sparkle in spades. Justified in that it took some time to get used to a completely new human body.
There are a few times that Fluttershy gets hit with things thrown at her.
Dangerously Genre Savvy: Sunset Shimmer at least gets points for trying, but in the film proper she doesn't really qualify. Prior to Twilight's arrival, she sabotaged the friendship of the Mane Five to make sure they couldn't threaten her efforts to get the crown (in the same way that Twilight does once they're reunited).
Designated Love Interest: Flash Sentry has been accused by many of being nothing more than an excuse for Twilight to have a crush on someone. Are they wrong? Well...
Deus ex Machina: The climax reveals that Twilight doesn't even need the other five elements to be present to tap into their power; Seems like the power of the bond Twilight shared with the human versions of her friends is sufficient to not only allow Twilight to reclaim the powers of the Element of Magic but also summon the powers of the other five elements, which has no basis in the show's actual canon and was never alluded to previous to its use.
Dope Slap: Spike does this to Twilight when Twilight says "everypony" instead of "everybody" while talking with Human!Fluttershy.
Easily Forgiven: Having to rebuild the school's entryway is a pretty light punishment for magically brainwashing the entire student body in a bid to conquer an alternate reality, all things considered.
Effortless Amazonian Lift: Two jocks help Twilight lift a nearby table, then stop and look on slack-jawed as Applejack passes by easily carrying a similar-sized table under one arm.
Entertainingly Wrong: Human!Pinkie Pie initially suspects that Twilight Sparkle is hiding something... namely, that she has psychic powers.
Even Evil Has Standards: Sunset admits this after she has Snips and Snails kidnap Spike to get Twilight's attention, then immediately releases him when the chase leads to her. She never intended to do anything, it just seemed the most expedient method to get Twilight to meet her.
Evil Cannot Comprehend Good: Sunset threatens to destroy the portal to Equestria if Twilight doesn't give up her crown, but this fails because Twilight would rather be stuck forever in an alternate reality than give Sunset the power of her element.
Expendable Alternate Universe: Subverted. Twilight refuses to leave Sunset loose to do whatever she wants in the human world with the Element of Magic because she knows if she left, there'd be no-one to stop her.
Fatal Flaw: Sunset Shimmer's is Greed and Pride. When she was still Celestia's star pupil, things weren't progressing as quickly as she wanted them to, so she became bitter and angry. She eventually found the magic mirror and disappeared from Equestria. On the other side, she established herself as the Alpha Bitch of the school. Not content with controlling the school, she stole Twilight's crown so she could amass an army of brainwashed teenagers with which to take over Equestria.
Fisher Kingdom: Twilight's arrival in the human world is accompanied by her transformation into a human herself.
Fish out of Water: Unsurprisingly, Twilight Sparkle has some trouble adapting to being human.
Flanderization: Due to the film's run time needing to fit in a lot of its own plot, the personalities of the alternate counterparts of Twilight's friends don't get a lot of depth. We see enough of them to recognize they're mostly the same as the originals and their core personality traits as such shine through, but by the end of the film their characterization is limited to "The Mane Six as humans".
Flash Mob: The Humane Six set up one in the school cafeteria to get everyone to vote for Twilight.
Sunset Shimmer hints at her plan early on when she claims that Twilight wouldn't have been much safer in Equestria than she is in the human world.
When Snips remarks, "Yeah! In Equestria..." it seems like he's just blindly repeating Sunset Shimmer without really knowing what she's talking about. However, this hints that Snips and Snails were fully aware of Sunset Shimmer's plan to take over an alternate world, which was confirmed when they were turned into demons along with Sunset.
The entire movie turns out to be foreshadowing for the Reflections arc of the IDW comic, as it hints at the existence of The Multiverse. Remember how Celestia was worried about Twilight disturbing the balance of the alternate world? There's a reason for that...
Gale-Force Sound: Human!Pinkie does this with a plastic megaphone during the cafeteria song.
Genre Savvy: Twilight knows that the explanation that she is actually a pony princess from another world looking for a crown that just so happens to resemble the Fall Formal prize is a too far-fetched story to believe, comparing it to what would happen if a human tried explaining their own origins if they came to Equestria. Twilight's suspicions are confirmed when even the rest of the girls don't believe it either, even after Pinkie correctly guesses it, but they quickly change their minds when Spike talks.
Getting Crap Past the Radar: Early on, Human!Twilight trips and falls onto all fours as the football jock walks by. In the background, he turns around to look while grinning.
Girliness Upgrade: Compared to the main show, Equestria Girls is much more obviously "girly", most infamously with Applejack and Rainbow Dash, who wear skirts (to the many cries of Ruined Forever by fans). However, the personalities and roles of Twilight and her friends' human counterparts remained pretty much the same as in the main series.
Good Wings, Evil Wings: The Big Bad and her minions with bat wings vs Twilight, Rainbow Dash, and Fluttershy's feathered wings after both groups use Twilight's crown to transform.
High School Dance: The Fall Formal; naturally, the portal closes the night it occurs. A similar dance, the Spring Fling is also mentioned to have happened prior to the movie.
Hostage for MacGuffin: Sunset attempts a twist on this by threatening to destroy the portal to Equestria if Twilight doesn't surrender her crown.
"How Did You Know?" "I Didn't.": Twice, both involving Pinkie: First, when Twilight prepares to confess to her human friends who she really is and what she's doing there, Human!Pinkie says it for her instead, uncannily right down to the very last detail; Pony!Pinkie later does the exact same thing when Twilight appears to have a crush on a guard pony because he reminds her of Flash Sentry. Both times, an exchange like this happens:
Twilight Sparkle: How did you know about that? Pinkie Pie: Just a hunch!
How Do I Shot Web?: In contrast to the ending of the season 3 finale, Twilight can't seem to manage to fly more than a few feet off the ground, and only then for a few seconds. Getting her wings to remain folded during sleep is also an issue. Likewise, adapting to walking as a human takes some time.
Human-Focused Adaptation: The first My Little Pony G4 movie, the first theatrically premierednote A limited 200-or-so screen release provided by cinema advertising fulfiller ScreenvisionMy Little Pony movie since 1986... and the plot is about human versions of ponies. Although it isn't played totally straight since the characters have been transformed or re-imagined as humans, rather than introducing completely new human characters.
Humanity Ensues: The inhabitants of the other world are humans, albeit ones with technicolor skin. Ponies from Equestria get turned into one upon arrival as seen with Twilight Sparkle and Sunset Shimmer. Spike gets a variation on this by being transformed into a dog.
Applejack calls Pinkie Pie, Fluttershy, and Rarity out for bickering with each other for something the other supposedly did instead of moving on. When Rarity points out that Applejack still has a grudge against Rainbow Dash, AJ says it's different because RD made a liar out of her.
Sunset is this, as well. She confronts Fluttershy at one point, telling her she shouldn't take things that don't belong to her. This is, of course, after Sunset herself went through all the trouble of stealing Twilight's crown and stealing the cheap replica that would have been the crown for the Fall Formal.
Twilight, when Sunset threatens to destroy the portal back to Equestria if Twilight doesn't relinquish the crown. Fortunately, Sunset was bluffing.
For some odd reason, Sunset herself after everything is said and done. Though it may come off as a self-imposed punishment for her actions.
I Have No Idea What I'm Doing: When Spike questions how Twilight plans to become Princess of the Fall Formal, Twilight "enthusiastically" says, "I have no idea!"
The Illegible: When Twilight signs up for the fall formal, Pinkie Pie can barely read her writing. Of course, this is because she's not used to having hands and usually writes through unicorn telekinesis.
I Never Told You My Name: Twilight addresses the human versions of Pinkie Pie and Applejack by name despite them not saying anything beforehand. From this, Pinkie Pie erroneously concludes Twilight is psychic.
In Spite of a Nail: The human world, despite being basically magic-free and technological development being drastically greater than the pony world, has the human counterparts of the various characters taking on more or less similar roles. The Mane Five (sans Twilight) were all friends prior to Sunset Shimmer sabotaging their relationship, and have all fallen into roles fitting those of their counterparts. The Cutie Mark Crusaders are still friends, their desire for cutie marks translated here as similar ill-conceived attempts at being popular. Celestia and Luna run the school. Even Twilight's counterpart being absent fits with Twilight herself not coming to Ponyville until made to do so.
Magic variant: the mirror provides an appropriate wardrobe for Twilight in addition to the physical changes. Although when she realizes this, the fact that she's now human understandably freaks her out much more than the sudden apparition of clothes.
Human!Rarity later pulls an ordinary one Twilight, putting her in a new dress and a blonde wig in a flash so that the student body wouldn't recognize and mock her any longer after the embarassing videos of Twilight show up on the Net.
Both Twilight Sparkle and Sunset Shimmer are technically ponies from Equestria transformed into humans, and one of them had dated Flash Sentry (human) in the past while the other was being hit on by him, with implied reciprocation.
There's also Dog!Spike's obvious crush on Human!Rarity, as well as his crush on Pony!Rarity as a dragon.
In the Hood: Sunset Shimmer uses this while stealing the crown.
It's clear that the human versions of Twilight's friends feel this way when they realize Sunset Shimmer is the one who ruined their friendship. It's especially evident with Applejack as she guiltily admits she stopped talking to Rainbow Dash altogether instead of questioning her about that suspicious e-mail falsely saying the softball team would be coming to AJ's bake sale.
It's also apparent that when they pulled a Care Bear Stare against Demon!Sunset Shimmer, this was the result.
Keep Away: The girls toss the crown between each other to keep Snips and Snails from grabbing it for Sunset Shimmer. It backfires when Twilight fails to check her throw.
Kick the Dog: Sunset Shimmer pulls off this trope so many times, ranging from bullying to framing Twilight to attempted murder.
Knight of Cerebus: Believe it or not, Sunset Shimmer. Her bullying is by far the cruelest displayed by any character in the series proper so far. Not only that, once she gains her demoness form, she turns Snips and Snails into demons, mind controls the entire student body as her own personal army, and comes dangerously close to actually killing Twilight.
Made-for-TV Movie: Announcedassuch by John A. Frascotti attheHasbro New York Toy Fair 2013 Investor Event, though its premiere date, theatrical release date, and DVD & Blu-ray release date all turned out to precede its television airdate.
Morphic Resonance: Both Twilight and Spike still look like themselves when turned into a human and a dog, respectively; Spike even mentions it. Twilight keeps the same hair style and skin color as her pony form, and her cutie mark is embroidered on her skirt. The same can be said of all the characters with pony equivalents, but here it isn't through shapechange but alternate-universe counterparts.
Mr. Exposition: Celestia's only role in the entire movie is to just talk about who Sunset Shimmer was and what the mirror can do. Nothing else.
Mundane Utility: Subverted. Twilight learns that she doesn't have magic in the human world when she can't open doors with telekinesis.
Noodle Incident: What did happen at last year's Spring Fling, and what exactly was the heinous thing that Sunset Shimmer did to the girl running against her, strongly implied to be Rarity? For what it's worth, the Novelization goes into more detail on this: Sunset had Snips and Snails covertly record Rarity pretending that a mannequin at the boutique where she worked was a prince and asking "him" for a kiss.
Noodle People: The human forms, where Twilight's stomach is only slightly thicker than her neck. Averted with Celestia and Luna, who have more realistic proportions. Maybe it's just all the gangly teens?
No Ontological Inertia: Any lingering changes wrought in the human world by Twilight's crown are instantly reverted when the portal closes, much to the discomfort of Rainbow's tush.
The One Guy: With Spike being a (talking) dog in the Alternate Universe and no other major male character from the series appearing in this rolenote a human Big Macintosh makes a couple of cameos and Shining Armor is completely absent, Flash Sentry fills this spot in the movie.
Pilot Movie: One of Hasbro's "Equestria Girls" trademarks includes "Entertainment services, namely an on-going animated television series for children" and an article in Kidscreen magazine's February/March 2013 issue claimed that "A new companion series called Equestria Girls is set to air this spring, sending the pony heroes on a mission to a new world where they take on human form." The editor of Kidscreenstated that Equestria Girls is a completely separate show that will have no bearing on My Little Pony: Friendship Is Magic.
Poor Communication Kills: Sunset Shimmer's efforts to break up the human versions of Twilight's friends would have been easily foiled had they actually discussed what happened instead of pointing fingers at each other.
Portal Slam: Human!Pinkie smashes into the statue while chasing Twilight when she returns to Equestria.
The Power of Friendship: As expected of a spinoff to a series subtitled "Friendship Is Magic", friendship is central to the entire movie. The first thing Twilight has to do upon arriving in the human world is rebuild the friendships between the alt!Six, the Big Bad comes from a Friendless Background, and friendship is the key to defeating her, just to name a few of the most obvious ways it figures into the story.
Precious Puppy: Spike gets turned into a baby dog instead of a baby dragon. Human-world Rarity and Fluttershy find him adorable.
Prehensile Hair: Human!Pinkie can pop the cap off a bottle of cider with her forelock.
A Pupil of Mine Until He Turned to Evil: Sunset Shimmer was Celestia's pupil before Twilight Sparkle. Celestia explains that she ran away when she wasn't able to gain power as quickly as she wanted. The tie-in comic clarifies that it was her interest in the mirror, and her belief that it could somehow turn her into a princess, that eventually drove Celestia to suspend her studies since she was clearly too selfish to ever become one.
Putting the Band Back Together: At the start of the film, the human versions of the Mane Six all utterly despise each other for various reasons thanks to Sunset Shimmer, and Twilight has to convince them to let go of their hatred and get along. The only exception is Applejack and Pinkie Pie, who still seem to get along fine.
Race Against the Clock: Twilight only has three days to get her crown and get back to Equestria before the portal closes again for thirty moons (2.5 years).
Rage Breaking Point: The girls finally let out all their frustration after they all (sans Rainbow Dash) offer to help Twilight win the crown right after the latter's humiliation at the hands of Sunset Shimmer.
Rapid Fire No: Twilight delivers one after Flash asks her out to the Fall Formal, making it look like she rejected him. Her response was actually towards the Fall Formal being moved past the Celestial Deadline. She clears it up later.
Flash Sentry: One "no" would've been fine!
Rapunzel Hair: The Humane Six's hair is already long enough, but their hair grows even longer to simulate tails when they undergo their transformations.
Reality Is Unrealistic: A vending machine with peanut butter crackers? Actually — that's not entirely uncommon.
"The Reason You Suck" Speech: Upon realizing that Twilight has followed her into the human world, Sunset smugly tells her that she's had more than enough time to take over the school, and Twilight can't hope to stop her in a world she is utterly unfamiliar with. Twilight then returns the favor, telling Sunset that despite having the Element of Magic on her head, she completely failed to understand and wield its true power, a power which the Humane 6 were able to use even without Twilight wearing the crown.
The Resenter: Towards the end of the movie, it becomes clear that Big Bad Sunset Shimmer is jealous of Twilight Sparkle, because Twilight managed to earn and achieve everything Sunset could not: the power, adoration, and crown that Twilight had never asked for yet Sunset had always desired. Her reaction to the way Twilight's friends praise her as a princess and her insistence that the crown is hers, shows that resentment and inches her closer to her Villainous Breakdown.
Satellite Character: Spike is characterized in this film mainly by his incredible loyalty and devotion to Twilight. In a perfect example of Tropes Are Not Bad, he is also hilarious and plays a huge part in helping Twilight adapt to her new body and surroundings. He's not the biggest example, though; if anything, other characters fit this trope a little more.
Satellite Love Interest: Flash Sentry has received quite a bit of criticism for this reason. He looks nice, helps Twilight twice, plays a guitar, generally seems to be a Nice Guy, was Sunset Shimmer's ex-boyfriend, and his equine counterpart is a guard in Cadance's castle. And that's all we ever know about him.
Save Both Worlds: Twilight has to get her crown back from Sunset, both because it could do harm to that world and without it the other Elements have no power.
Script-Reading Doors: The front doors of Canterlot High are amazingly plot-sensitive. When Twilight walks into one it stays closed, but then the much smaller Spike is able to push it open with one paw. Later on, the same door stays closed when Spike pushes on it with his full weight.
Secret Test of Character: Rainbow Dash insists on a game of one on one soccer before she'll help Twilight. Rainbow Dash utterly curbstomps Twilight. She agrees to help anyway because the point wasn't to see if Twilight could beat her at soccer, but to see if Twilight had the resolve and the courage to face an obviously superior opponent if she needed to in order to do what she felt was important.
Ship Tease: In the human world, Twilight develops a crush on Flash Sentry and they dance together at the High School Dance. The movie ends with Twilight going back to Equestria and bumping into Flash's pony counterpart, a member of the Royal Guard. She blushes and her friends tease her a little. A tease, because Word of God says Flash Sentry won't be in Season 4 of FIM.
At the very beginning, when Pinkie is defining "nervicited", the transformation sound effect from Transformers is used as she uncurls from the ball. Her unfolding moves, while appearing awkward for a pony, resemble the transformation sequences of the Transformers as well.
The way Fluttershy introduces Twilight to the various high school cliques in the cafeteria is similar to the scene in Mean Girls.
Sunset Shimmer dismissively refers to Twilight and Spike as "Princess Celestia's prized student [...] and your little dog, too!"
One of the related videos on the YouTube clone when the CMC are checking their video's comments is from Pound Puppies.
When the Humane Six start cleaning up the gym, Twilight tosses out brooms, which the other five catch — except for Fluttershy who winces and turns her back, letting the broom hit her instead. Jayson Thiessen confirmed this as a shout-out to a similar scene from Arrested Development.
The aforementioned Sadistic Choice is very similar to the one Tachyon gave Ratchet in "Tools Of Destruction", both being offered to go to their true home and refusing because they know said home can protect themselves but if they leave there will be no one to stop the villain.
The beginning of the "Helping Twilight Win the Crown" number, with students banging on the cafeteria tables, may be a reference to thisCold Open from Cheers where the bar patrons start singing "We Will Rock You" the same way.
The Main Six's transformations are very reminiscent of Sailor Moon.
Two people who look similar to Tsubomi and Erika from Heart Catch Pretty Cure appear multiple times in the film. The Tsubomi lookalike can be clearly seen in the scene where Sunset Shimmer is about to tell Luna about how the gym got messed up. Then, the same character appears with the Erika lookalike in the cleaning-the-gym musical number.
Derpy's dance with a muffin in the end credits somewhat resembles how the person in Dumb Ways To Die who sold their kidneys danced.
The Stinger: At the very end of the credits, a certain blond-haired, gray-skinned, wall-eyed girl is seen dancing with a muffin.
Stock Scream: A Wilhelm scream is heard when Demon!Sunset attacks the school.
Stock Sound Effect: The laughter heard when the Humane Six arrive at the Canterlot High Fall Formal is the same clip of laughter heard at the end of Pinkie Pie's "Giggle at the Ghosties" song.
Taking the Bullet: During the final showdown with Sunset Shimmer, when Sunset throws a fireball at Twilight, all of the Humane Five leap around Twilight to shield her. Fortunately the Power of Friendship keeps them from getting hurt.
Talking Animal: Spike in the human world. This is apparently not normal there.
There Are No Adults: Played straight with the human world — aside from Principal Celestia and Vice-Principal Luna, the only other adults are cameos with no effect on the plot (Granny Smith, Cheerilee, and Mr. & Mrs. Cake). The absolute lack of parents is puzzling; the total absence of law enforcement after the giant fire-demon demolishes the front of the school totally boggles the mind.
Third Act Misunderstanding: When Flash Sentry asks Twilight out to the Fall Formal after it gets delayed a day, Twilight panics that she's going to miss her Celestial Deadline to return home and says "no" dozens of times. Flash, of course, mistakes this as a rejection. Downplayed, as it neither detracts from nor adds to their relationship, and Flash just asks again later, whereupon the confusion is cleared up.
This Is Something She's Got to Do Herself: Celestia insists that Twilight alone has to find her crown, since all of her friends going through the portal could upset the balance between the worlds (and the resulting twin scenario at least would have been quite awkward). Spike ends up diving through anyway. No explanation is given for why Twilight can't go back and forth between Equestria and the human world to get advice, though.
Title Drop: In the novelization, the narration occasionally called the human mane six "Equestria Girls".
Two-Teacher School: Canterlot High's only visible staff are Principal Celestia, Vice Principal Luna, Cheerilee who can be seen writing on a blackboard in a class and who helps Twilight in the library, and Granny Smith as a lunch lady. Other than that, there's way more students than these four faculty members.
Unusual Ears: Some promotional art and the dolls give the girls pony ears. They're part of a Magical Girl style transformation during the movie's climax and go away once Twilight returns home.
Unusually Uninteresting Sight: The film ends with the Humane Six and Sunset Shimmer going through magical transformations and having a showdown in full view of everybody. Note that this is a universe where magic is thought not to exist. The students and faculty seem to take this in stride, though they're a little weirded out by Spike.
Urban Fantasy: The contemporary, Earthlike alternate universe lacks magic, but the movie qualifies for this trope in a couple of ways:
The main character and villain get there by magic.
Vague Age: In Equestria, there is a pretty substantial age gap between Twilight and Sunset, about the same as Twilight and Shining Armor. On "Earth", they can pass for the same grade. Similarly, several ponies that are working adults in Equestria are still in High School in the other world.
Vengeful Vending Machine: Twilight's first encounter with a vending machine in the human world goes this way, not because the machine is broken, but she just doesn't know how to use one and nearly ends up bucking it in frustration.
Villains Blend in Better: Sunset Shimmer doesn't stand out like Twilight Sparkle does in the human world. This is justified as Sunset has already spent a lot of time getting to know the human world, probably the entire period of Twilight's apprenticeship to Celestia given the former's unfamiliarity with Sunset.
When Snips and Snails arrive in the library to spy on Twilight, they sneak off to get good vantage points. Snips slides along the wall, opening and closing his legs like scissors, and Snails slides along the ground, like a snail.
When the Humane Six go through their transformation, their hair styles gain pony tails.
Sunset Shimmer frames Twilight for the destruction of the Fall Formal decorations through an incredibly crude forgery using cutouts and a photocopier. Even MS Paint would have produced equally convincing forgeries without leaving evidence for Flash Sentry to find. Gets doubly weird when you remember that she's already used a video editing program comparable to Adobe Premiere.
The Novelization does have them using photo editing technology, but they still leave evidence behind in the form of the original photos so Luna can compare them to the altered ones.
We Used to Be Friends: The human versions of the Mane 6 (minus Twilight) used to be friends with one another until events orchestrated by Sunset Shimmer drove them apart.
Winged Humanoid: Twilight's crown transforms herself, Human!Fluttershy and Human!Rainbow Dash this way.
Youthful Freckles: Human Cheerilee gains these (normally lacking on her pony form). It is most likely to help establish her age as a younger teacher, not too far outside the age that the Humane Six as humans would be, compared to the more mature-looking Principals Celestia and Luna.